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The map label for this gene is pfkA2 [H]

Identifier: 146302318

GI number: 146302318

Start: 5550890

End: 5551876

Strand: Reverse

Name: pfkA2 [H]

Synonym: Fjoh_4591

Alternate gene names: 146302318

Gene position: 5551876-5550890 (Counterclockwise)

Preceding gene: 146302319

Following gene: 146302317

Centisome position: 91.06

GC content: 36.68

Gene sequence:

>987_bases
ATGTCAAAAACAATAAAAAAAGTAGGTGTTCTTACCTCAGGAGGAGACTCACCGGGAATGAATGCTGCAATACGATCAGT
TGTTCGAACATGTGCTTATCATAATATAGAATGCATAGGAATTTATAGAGGGTATCAAGGAATGATCGAAGGTGATTTTA
AAGAAATGGGCCCTCGAAGCGTTAATAATATTGTAAATAAAGGAGGAACGATCCTGAAATCGGCTCGTTCTGTAGACTTT
AGAACCCCGGAAGGCCGTAAAAAAGCTCACGAAAATCTTCTTAAAGCCGGAATTGATGCTTTAGTTGTTATTGGAGGAGA
CGGAAGTTTTACCGGAGGTTTAATTTTTAACTCAGAATATGATTTTCCTGTAATGGGAATTCCAGGTACAATTGATAATG
ATATTTATGGTACAAGCTTTACTTTAGGTTATGACACTGCTTTAAATACTGTTGTTGACTGTATAGATAAAATTAGAGAT
ACTGCAAGTTCACATAACCGTTTATTCTTTGTAGAGGTTATGGGTAGAGATGCAGGTCACATCGCACTTAATGCCGGAAT
TGGAGCAGGTGCAGAAGAAATCCTTATTCCTGAAGAAGATTTAGGTCTTGACAGATTGTTAGACTCTCTTCAAAAAAGTA
AAGCTTCAGGAAAATCATCAAGTATAGTAGTTATTGCCGAAGGTGATAAAATTGGTAAAAACGTATTCGAATTAAAAGAT
TATGTTGAAGCTAATTTACCTGAATACGACGTAAGAGTATCTGTTTTAGGACACATGCAGCGTGGTGGTTCGCCATCTTG
TTTTGACCGTGTTCTGGCAAGTCGTCTTGGTGTAAAAGCAGTTGAGTCTTTAATTGAAGGAAAATCAAACTACATGGTTG
GTCTTAAGGAAGATAAAGTTGTTTTAACTCCGCTGGAGCAGGCAATTAAAGGTAAATCTGAAATTGATAGAGAATTATTG
AGAGTGTCAGACATTATGTCTACATAA

Upstream 100 bases:

>100_bases
AACGAAAACGTTTGAATTTAACAGATTTTATTAAATTATAATAAATATAACCGCAGAAGTGGTGAATGTTTGCTAAATTT
ACAACTTTAATACTTAAATA

Downstream 100 bases:

>100_bases
TTATAAAGATAAAAGTTTAAAAGAGAAGAAGTTTATTGTGAGGAAATTAGACTTTGAATTAATATAATAAAAACAAAAGC
AAAAATTTAGTAAAATGTCA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 2; Phosphohexokinase 2 [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSKTIKKVGVLTSGGDSPGMNAAIRSVVRTCAYHNIECIGIYRGYQGMIEGDFKEMGPRSVNNIVNKGGTILKSARSVDF
RTPEGRKKAHENLLKAGIDALVVIGGDGSFTGGLIFNSEYDFPVMGIPGTIDNDIYGTSFTLGYDTALNTVVDCIDKIRD
TASSHNRLFFVEVMGRDAGHIALNAGIGAGAEEILIPEEDLGLDRLLDSLQKSKASGKSSSIVVIAEGDKIGKNVFELKD
YVEANLPEYDVRVSVLGHMQRGGSPSCFDRVLASRLGVKAVESLIEGKSNYMVGLKEDKVVLTPLEQAIKGKSEIDRELL
RVSDIMST

Sequences:

>Translated_328_residues
MSKTIKKVGVLTSGGDSPGMNAAIRSVVRTCAYHNIECIGIYRGYQGMIEGDFKEMGPRSVNNIVNKGGTILKSARSVDF
RTPEGRKKAHENLLKAGIDALVVIGGDGSFTGGLIFNSEYDFPVMGIPGTIDNDIYGTSFTLGYDTALNTVVDCIDKIRD
TASSHNRLFFVEVMGRDAGHIALNAGIGAGAEEILIPEEDLGLDRLLDSLQKSKASGKSSSIVVIAEGDKIGKNVFELKD
YVEANLPEYDVRVSVLGHMQRGGSPSCFDRVLASRLGVKAVESLIEGKSNYMVGLKEDKVVLTPLEQAIKGKSEIDRELL
RVSDIMST
>Mature_327_residues
SKTIKKVGVLTSGGDSPGMNAAIRSVVRTCAYHNIECIGIYRGYQGMIEGDFKEMGPRSVNNIVNKGGTILKSARSVDFR
TPEGRKKAHENLLKAGIDALVVIGGDGSFTGGLIFNSEYDFPVMGIPGTIDNDIYGTSFTLGYDTALNTVVDCIDKIRDT
ASSHNRLFFVEVMGRDAGHIALNAGIGAGAEEILIPEEDLGLDRLLDSLQKSKASGKSSSIVVIAEGDKIGKNVFELKDY
VEANLPEYDVRVSVLGHMQRGGSPSCFDRVLASRLGVKAVESLIEGKSNYMVGLKEDKVVLTPLEQAIKGKSEIDRELLR
VSDIMST

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=348, Percent_Identity=40.5172413793103, Blast_Score=241, Evalue=5e-64,
Organism=Homo sapiens, GI11321601, Length=348, Percent_Identity=40.5172413793103, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI266453619, Length=348, Percent_Identity=39.6551724137931, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI266453768, Length=348, Percent_Identity=39.6551724137931, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI266453748, Length=348, Percent_Identity=39.6551724137931, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI4505749, Length=348, Percent_Identity=39.6551724137931, Blast_Score=237, Evalue=1e-62,
Organism=Escherichia coli, GI1790350, Length=306, Percent_Identity=47.3856209150327, Blast_Score=266, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI25147584, Length=312, Percent_Identity=42.6282051282051, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17558788, Length=310, Percent_Identity=37.0967741935484, Blast_Score=198, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI72003601, Length=339, Percent_Identity=32.1533923303835, Blast_Score=157, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6321679, Length=345, Percent_Identity=38.5507246376812, Blast_Score=230, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6323861, Length=346, Percent_Identity=34.393063583815, Blast_Score=206, Evalue=6e-54,
Organism=Drosophila melanogaster, GI28573326, Length=352, Percent_Identity=38.3522727272727, Blast_Score=237, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24652337, Length=352, Percent_Identity=38.9204545454545, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI17647809, Length=348, Percent_Identity=39.0804597701149, Blast_Score=235, Evalue=2e-62,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35298; Mature: 35167

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTIKKVGVLTSGGDSPGMNAAIRSVVRTCAYHNIECIGIYRGYQGMIEGDFKEMGPRS
CCCCHHHHCCEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCHHHCCCHH
VNNIVNKGGTILKSARSVDFRTPEGRKKAHENLLKAGIDALVVIGGDGSFTGGLIFNSEY
HHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEECCEEECCCC
DFPVMGIPGTIDNDIYGTSFTLGYDTALNTVVDCIDKIRDTASSHNRLFFVEVMGRDAGH
CCEEECCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCE
IALNAGIGAGAEEILIPEEDLGLDRLLDSLQKSKASGKSSSIVVIAEGDKIGKNVFELKD
EEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHH
YVEANLPEYDVRVSVLGHMQRGGSPSCFDRVLASRLGVKAVESLIEGKSNYMVGLKEDKV
HHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCEEEEECCCCE
VLTPLEQAIKGKSEIDRELLRVSDIMST
EECCHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKTIKKVGVLTSGGDSPGMNAAIRSVVRTCAYHNIECIGIYRGYQGMIEGDFKEMGPRS
CCCHHHHCCEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCHHHCCCHH
VNNIVNKGGTILKSARSVDFRTPEGRKKAHENLLKAGIDALVVIGGDGSFTGGLIFNSEY
HHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEECCEEECCCC
DFPVMGIPGTIDNDIYGTSFTLGYDTALNTVVDCIDKIRDTASSHNRLFFVEVMGRDAGH
CCEEECCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCE
IALNAGIGAGAEEILIPEEDLGLDRLLDSLQKSKASGKSSSIVVIAEGDKIGKNVFELKD
EEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHH
YVEANLPEYDVRVSVLGHMQRGGSPSCFDRVLASRLGVKAVESLIEGKSNYMVGLKEDKV
HHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCEEEEECCCCE
VLTPLEQAIKGKSEIDRELLRVSDIMST
EECCHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]