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The map label for this gene is gapA [H]

Identifier: 146302317

GI number: 146302317

Start: 5549791

End: 5550795

Strand: Reverse

Name: gapA [H]

Synonym: Fjoh_4590

Alternate gene names: 146302317

Gene position: 5550795-5549791 (Counterclockwise)

Preceding gene: 146302318

Following gene: 146302316

Centisome position: 91.04

GC content: 36.22

Gene sequence:

>1005_bases
ATGTCAAAAGTAAAATTAGGAATAAACGGATTTGGACGTATCGGAAGAATCGTTTTTAGAGAGTCTTTCAATAGAGATAA
TGTAGAAGTTGTAGCGATCAACGATTTATTAGACGTAGATCACTTAGCTTACTTATTAAAATATGATTCAGTTCACGGTC
GTTTCAACGGAACTGTTGAAGTTAAAGAAGGAAAATTGTATGTAAACGGAAGAAACATCCGTATCACTGCAGAAAGAAAT
CCTGCTGACTTAAAATGGAACGAAGTTGATGTTGATGTAGTAGCAGAATGTACTGGTATCTTCACAACTATCGAAACTGC
AAGCGAGCACTTAAAAGGAGGAGCTAAAAAAGTTATCATTTCTGCTCCATCTGCTGATGCTCCAATGTTTGTAATGGGAG
TTAACCACGAAACTGCAAAAGCTTCTGATTTAGTTGTTTCTAACGCTTCTTGTACTACAAACTGTTTAGCTCCTTTAGCT
AAAGTTATCCACGATAATTTCGAAATCGTTGAAGGTTTAATGACAACTGTTCACGCAACTACTTCAACTCAAATGACTGC
TGACGGACCTTCTAGAAAAGACTGGAGAGGTGGACGTGCAGCTGCAATCAACATCATTCCTTCTTCAACAGGTGCTGCTA
AAGCGGTTGGAAAAGTTATCCCATCTTTAAATGGAAAATTAACTGGAATGTCTTTCCGTGTACCTACTGCTGACGTTTCT
GTTGTAGATTTAACTGTAAAAGTTGCTAAAGAAACTACATACGAAGAAATCTTAGCTGTATTGAAAAAAGCTTCAGAAAA
CGAAATGAAAGGTATCTTAGGATATACTGAAGATGCAGTTGTTTCTCAAGACTTTATTTCTGATAAAAGAACTTCAATCG
TTGATGCTGGTGCAGGAATCGGATTAAATTCTACTTTCTTCAAATTAGTATCTTGGTATGACAATGAGTACGGATACTCA
AGCAAATTAATTGATTTGTCTGTGCATATCGCAGGTTTAAAATAA

Upstream 100 bases:

>100_bases
ACATAATTATAAAGATAAAAGTTTAAAAGAGAAGAAGTTTATTGTGAGGAAATTAGACTTTGAATTAATATAATAAAAAC
AAAAGCAAAAATTTAGTAAA

Downstream 100 bases:

>100_bases
TATTATATATTTTTGCAAAATCCCGTTATCATCAAAGTAACGGGATTTTCTTATTTTGTTACGTCTTTAATTAATGTAAA
AACTACACTTATTGAGGAAA

Product: glyceraldehyde-3-phosphate dehydrogenase, type I

Products: NA

Alternate protein names: GAPDH-A [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSKVKLGINGFGRIGRIVFRESFNRDNVEVVAINDLLDVDHLAYLLKYDSVHGRFNGTVEVKEGKLYVNGRNIRITAERN
PADLKWNEVDVDVVAECTGIFTTIETASEHLKGGAKKVIISAPSADAPMFVMGVNHETAKASDLVVSNASCTTNCLAPLA
KVIHDNFEIVEGLMTTVHATTSTQMTADGPSRKDWRGGRAAAINIIPSSTGAAKAVGKVIPSLNGKLTGMSFRVPTADVS
VVDLTVKVAKETTYEEILAVLKKASENEMKGILGYTEDAVVSQDFISDKRTSIVDAGAGIGLNSTFFKLVSWYDNEYGYS
SKLIDLSVHIAGLK

Sequences:

>Translated_334_residues
MSKVKLGINGFGRIGRIVFRESFNRDNVEVVAINDLLDVDHLAYLLKYDSVHGRFNGTVEVKEGKLYVNGRNIRITAERN
PADLKWNEVDVDVVAECTGIFTTIETASEHLKGGAKKVIISAPSADAPMFVMGVNHETAKASDLVVSNASCTTNCLAPLA
KVIHDNFEIVEGLMTTVHATTSTQMTADGPSRKDWRGGRAAAINIIPSSTGAAKAVGKVIPSLNGKLTGMSFRVPTADVS
VVDLTVKVAKETTYEEILAVLKKASENEMKGILGYTEDAVVSQDFISDKRTSIVDAGAGIGLNSTFFKLVSWYDNEYGYS
SKLIDLSVHIAGLK
>Mature_333_residues
SKVKLGINGFGRIGRIVFRESFNRDNVEVVAINDLLDVDHLAYLLKYDSVHGRFNGTVEVKEGKLYVNGRNIRITAERNP
ADLKWNEVDVDVVAECTGIFTTIETASEHLKGGAKKVIISAPSADAPMFVMGVNHETAKASDLVVSNASCTTNCLAPLAK
VIHDNFEIVEGLMTTVHATTSTQMTADGPSRKDWRGGRAAAINIIPSSTGAAKAVGKVIPSLNGKLTGMSFRVPTADVSV
VDLTVKVAKETTYEEILAVLKKASENEMKGILGYTEDAVVSQDFISDKRTSIVDAGAGIGLNSTFFKLVSWYDNEYGYSS
KLIDLSVHIAGLK

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=332, Percent_Identity=64.4578313253012, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI7657116, Length=326, Percent_Identity=57.361963190184, Blast_Score=390, Evalue=1e-108,
Organism=Escherichia coli, GI1788079, Length=329, Percent_Identity=68.9969604863222, Blast_Score=469, Evalue=1e-134,
Organism=Escherichia coli, GI1789295, Length=334, Percent_Identity=39.5209580838323, Blast_Score=242, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI32566163, Length=341, Percent_Identity=62.1700879765396, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17534677, Length=341, Percent_Identity=63.049853372434, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17568413, Length=341, Percent_Identity=62.1700879765396, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17534679, Length=341, Percent_Identity=62.4633431085044, Blast_Score=423, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6321631, Length=329, Percent_Identity=62.3100303951368, Blast_Score=427, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6322468, Length=329, Percent_Identity=62.0060790273556, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6322409, Length=329, Percent_Identity=59.8784194528875, Blast_Score=419, Evalue=1e-118,
Organism=Drosophila melanogaster, GI17933600, Length=323, Percent_Identity=64.7058823529412, Blast_Score=429, Evalue=1e-120,
Organism=Drosophila melanogaster, GI18110149, Length=323, Percent_Identity=64.7058823529412, Blast_Score=429, Evalue=1e-120,
Organism=Drosophila melanogaster, GI85725000, Length=323, Percent_Identity=65.015479876161, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI22023983, Length=323, Percent_Identity=65.015479876161, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI19922412, Length=325, Percent_Identity=59.0769230769231, Blast_Score=398, Evalue=1e-111,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 35987; Mature: 35856

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVKLGINGFGRIGRIVFRESFNRDNVEVVAINDLLDVDHLAYLLKYDSVHGRFNGTVE
CCEEEECCCCCCHHHHHEEHHCCCCCCEEEEEECCHHCHHHHHHHHHCCCCCCEECCEEE
VKEGKLYVNGRNIRITAERNPADLKWNEVDVDVVAECTGIFTTIETASEHLKGGAKKVII
EECCEEEECCCEEEEEECCCCCCCEECEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
SAPSADAPMFVMGVNHETAKASDLVVSNASCTTNCLAPLAKVIHDNFEIVEGLMTTVHAT
ECCCCCCCEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHEEC
TSTQMTADGPSRKDWRGGRAAAINIIPSSTGAAKAVGKVIPSLNGKLTGMSFRVPTADVS
CCCEEECCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCEEEEEEEECCCCCEE
VVDLTVKVAKETTYEEILAVLKKASENEMKGILGYTEDAVVSQDFISDKRTSIVDAGAGI
EEEEEEEEHHCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHCCCHHEEEECCCCC
GLNSTFFKLVSWYDNEYGYSSKLIDLSVHIAGLK
CCCHHHHHHHHHHCCCCCCCCEEEEEEEEEEECC
>Mature Secondary Structure 
SKVKLGINGFGRIGRIVFRESFNRDNVEVVAINDLLDVDHLAYLLKYDSVHGRFNGTVE
CEEEECCCCCCHHHHHEEHHCCCCCCEEEEEECCHHCHHHHHHHHHCCCCCCEECCEEE
VKEGKLYVNGRNIRITAERNPADLKWNEVDVDVVAECTGIFTTIETASEHLKGGAKKVII
EECCEEEECCCEEEEEECCCCCCCEECEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
SAPSADAPMFVMGVNHETAKASDLVVSNASCTTNCLAPLAKVIHDNFEIVEGLMTTVHAT
ECCCCCCCEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHEEC
TSTQMTADGPSRKDWRGGRAAAINIIPSSTGAAKAVGKVIPSLNGKLTGMSFRVPTADVS
CCCEEECCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCEEEEEEEECCCCCEE
VVDLTVKVAKETTYEEILAVLKKASENEMKGILGYTEDAVVSQDFISDKRTSIVDAGAGI
EEEEEEEEHHCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHCCCHHEEEECCCCC
GLNSTFFKLVSWYDNEYGYSSKLIDLSVHIAGLK
CCCHHHHHHHHHHCCCCCCCCEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]