BacMap

Definition	Flavobacterium johnsoniae UW101 chromosome, complete genome.
Accession	NC_009441
Length	6,096,872

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The map label for this gene is hdhA [C]

Identifier: 146302174

GI number: 146302174

Start: 5363862

End: 5364614

Strand: Direct

Name: hdhA [C]

Synonym: Fjoh_4442

Alternate gene names: 146302174

Gene position: 5363862-5364614 (Clockwise)

Preceding gene: 146302173

Following gene: 146302175

Centisome position: 87.98

GC content: 39.04

Gene sequence:

>753_bases
ATGAAAAAATTAAACAACAAAACAGCAGTAATTACAGGCTCAGTTAGAGGATTAGGAAAAGCAATTGCAGAAAGATATGC
TGCAATGGGTGCAGACATCGTAATTAATTATTCGAAAGACAAAGCTTCGGCAGATGCGGTTTTAAGCACTATTAAAGCAA
TGGGGGTAAAAGCAATTGCTGTTCAGGCTGATGTGAGCAAAGTGGCCGATTTGGAAAGATTGTTTGCTGAGGCAAAAGCG
GCATTTGGAAAAATTGATATTGTAGTGGCCAATGCAGGAATCGAAATGGTAGAAGTTCCTGTTACTGAATTTACAGAAGA
ACAATTTGATAAAGTATTTTCTATTAATACTAAAGGAACTTATTTTACGCTTCAGCAGGCTGCGAAACACATTGAAGATA
ACGGTAGAATTATTTACATCGCTTCGAGTACTACTTCTTTCCCCGTTCCCGGAATGGCTGTTTACGGAGGAAGCAAAACT
ACGCCAAGATATTTAGTCGATATTTTATCTAAAGAAATAGGACATCGAGGTGTAACAGTGAACTCAATTATCCCATTTGC
TGTAGATCACTCAGGAATATTTGCAGAGGCAGACAGCTATCCGGAACTAAGAAAACAATTGTTAGACAGCTGCCCGATGA
GACGATTGGCAGAAGTGGAAGACGTAGCAAATGCGGCTGAATTTTTCGCAAGTGATTTATCTTCTTTTGTAAGCGGACAA
CACTTATTGGTAAATGGCGGAGCTACAAACTAA

Upstream 100 bases:

>100_bases
GCAATGCTTTATTCCTTGCAATATTCGACGCTTACCACCGCCATACATTTGTCCTGTCAAATTAGAACAACATTTTAAAA
TCATTTAAAAAATTAAAATC

Downstream 100 bases:

>100_bases
AATCAAACCGTCTATGAAAACATTAATAATGGGACTGTTTTTTACTTGTTTTGCCTGCGGGCATCCCACAAATAAATTAG
AGAAAGTGAATTTAGATAAA

Product: short-chain dehydrogenase/reductase SDR

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: ORFC [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MKKLNNKTAVITGSVRGLGKAIAERYAAMGADIVINYSKDKASADAVLSTIKAMGVKAIAVQADVSKVADLERLFAEAKA
AFGKIDIVVANAGIEMVEVPVTEFTEEQFDKVFSINTKGTYFTLQQAAKHIEDNGRIIYIASSTTSFPVPGMAVYGGSKT
TPRYLVDILSKEIGHRGVTVNSIIPFAVDHSGIFAEADSYPELRKQLLDSCPMRRLAEVEDVANAAEFFASDLSSFVSGQ
HLLVNGGATN

Sequences:

>Translated_250_residues
MKKLNNKTAVITGSVRGLGKAIAERYAAMGADIVINYSKDKASADAVLSTIKAMGVKAIAVQADVSKVADLERLFAEAKA
AFGKIDIVVANAGIEMVEVPVTEFTEEQFDKVFSINTKGTYFTLQQAAKHIEDNGRIIYIASSTTSFPVPGMAVYGGSKT
TPRYLVDILSKEIGHRGVTVNSIIPFAVDHSGIFAEADSYPELRKQLLDSCPMRRLAEVEDVANAAEFFASDLSSFVSGQ
HLLVNGGATN
>Mature_250_residues
MKKLNNKTAVITGSVRGLGKAIAERYAAMGADIVINYSKDKASADAVLSTIKAMGVKAIAVQADVSKVADLERLFAEAKA
AFGKIDIVVANAGIEMVEVPVTEFTEEQFDKVFSINTKGTYFTLQQAAKHIEDNGRIIYIASSTTSFPVPGMAVYGGSKT
TPRYLVDILSKEIGHRGVTVNSIIPFAVDHSGIFAEADSYPELRKQLLDSCPMRRLAEVEDVANAAEFFASDLSSFVSGQ
HLLVNGGATN

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI5031737, Length=249, Percent_Identity=30.9236947791165, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI32483357, Length=249, Percent_Identity=31.7269076305221, Blast_Score=115, Evalue=2e-26,
Organism=Homo sapiens, GI126723750, Length=252, Percent_Identity=27.7777777777778, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI15277342, Length=259, Percent_Identity=30.1158301158301, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI33667109, Length=183, Percent_Identity=31.6939890710383, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI7705925, Length=247, Percent_Identity=27.9352226720648, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI126723191, Length=182, Percent_Identity=33.5164835164835, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI19923817, Length=258, Percent_Identity=24.8062015503876, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI4504505, Length=195, Percent_Identity=28.7179487179487, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI40254992, Length=248, Percent_Identity=26.6129032258064, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI10190704, Length=248, Percent_Identity=26.6129032258064, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4503301, Length=231, Percent_Identity=25.974025974026, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI59889578, Length=225, Percent_Identity=27.1111111111111, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI1787905, Length=252, Percent_Identity=32.9365079365079, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787335, Length=244, Percent_Identity=31.9672131147541, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI2367365, Length=251, Percent_Identity=29.8804780876494, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1789208, Length=257, Percent_Identity=31.1284046692607, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082100, Length=260, Percent_Identity=31.1538461538462, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788459, Length=250, Percent_Identity=28, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87082160, Length=249, Percent_Identity=30.1204819277108, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI2367175, Length=245, Percent_Identity=28.5714285714286, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789378, Length=250, Percent_Identity=29.2, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790717, Length=248, Percent_Identity=26.6129032258064, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1786812, Length=256, Percent_Identity=26.953125, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1787820, Length=179, Percent_Identity=28.4916201117318, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789057, Length=257, Percent_Identity=25.2918287937743, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787545, Length=258, Percent_Identity=26.3565891472868, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17560676, Length=250, Percent_Identity=28.4, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17561402, Length=251, Percent_Identity=29.4820717131474, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI115534694, Length=248, Percent_Identity=30.6451612903226, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17555706, Length=247, Percent_Identity=27.5303643724696, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25147288, Length=249, Percent_Identity=29.3172690763052, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193204405, Length=268, Percent_Identity=30.5970149253731, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17560150, Length=265, Percent_Identity=29.811320754717, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17562908, Length=275, Percent_Identity=26.5454545454545, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17564282, Length=202, Percent_Identity=29.2079207920792, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17568967, Length=195, Percent_Identity=29.7435897435897, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17508895, Length=198, Percent_Identity=28.2828282828283, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193203117, Length=210, Percent_Identity=30, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193203115, Length=211, Percent_Identity=29.8578199052133, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17508651, Length=250, Percent_Identity=28.4, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17544670, Length=272, Percent_Identity=26.8382352941176, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560220, Length=266, Percent_Identity=27.8195488721804, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17562990, Length=264, Percent_Identity=27.6515151515151, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI72000259, Length=266, Percent_Identity=26.6917293233083, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17531453, Length=268, Percent_Identity=27.6119402985075, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17559104, Length=271, Percent_Identity=26.5682656826568, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=278, Percent_Identity=25.8992805755396, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71994600, Length=251, Percent_Identity=25.4980079681275, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17538486, Length=266, Percent_Identity=24.812030075188, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71994604, Length=170, Percent_Identity=27.0588235294118, Blast_Score=64, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17560332, Length=271, Percent_Identity=25.830258302583, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6322861, Length=214, Percent_Identity=31.3084112149533, Blast_Score=76, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324126, Length=250, Percent_Identity=24.8, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355319, Length=253, Percent_Identity=30.0395256916996, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI23397609, Length=251, Percent_Identity=31.4741035856574, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI28571526, Length=256, Percent_Identity=30.078125, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24639444, Length=254, Percent_Identity=30.3149606299213, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24644339, Length=247, Percent_Identity=29.1497975708502, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24643142, Length=245, Percent_Identity=29.3877551020408, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21357041, Length=257, Percent_Identity=29.5719844357977, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24640492, Length=211, Percent_Identity=28.436018957346, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18859837, Length=218, Percent_Identity=29.3577981651376, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 26757; Mature: 26757

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLNNKTAVITGSVRGLGKAIAERYAAMGADIVINYSKDKASADAVLSTIKAMGVKAIA
CCCCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEE
VQADVSKVADLERLFAEAKAAFGKIDIVVANAGIEMVEVPVTEFTEEQFDKVFSINTKGT
EEHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCEEEECCHHHHHHHHHHHEEECCCCCC
YFTLQQAAKHIEDNGRIIYIASSTTSFPVPGMAVYGGSKTTPRYLVDILSKEIGHRGVTV
EEEHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEE
NSIIPFAVDHSGIFAEADSYPELRKQLLDSCPMRRLAEVEDVANAAEFFASDLSSFVSGQ
CCCCCEEECCCCCEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HLLVNGGATN
EEEEECCCCC
>Mature Secondary Structure
MKKLNNKTAVITGSVRGLGKAIAERYAAMGADIVINYSKDKASADAVLSTIKAMGVKAIA
CCCCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEE
VQADVSKVADLERLFAEAKAAFGKIDIVVANAGIEMVEVPVTEFTEEQFDKVFSINTKGT
EEHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCEEEECCHHHHHHHHHHHEEECCCCCC
YFTLQQAAKHIEDNGRIIYIASSTTSFPVPGMAVYGGSKTTPRYLVDILSKEIGHRGVTV
EEEHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEE
NSIIPFAVDHSGIFAEADSYPELRKQLLDSCPMRRLAEVEDVANAAEFFASDLSSFVSGQ
CCCCCEEECCCCCEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HLLVNGGATN
EEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8868443 [H]