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The map label for this gene is prrB [H]

Identifier: 146302129

GI number: 146302129

Start: 5298563

End: 5299807

Strand: Direct

Name: prrB [H]

Synonym: Fjoh_4394

Alternate gene names: 146302129

Gene position: 5298563-5299807 (Clockwise)

Preceding gene: 146302128

Following gene: 146302130

Centisome position: 86.91

GC content: 32.05

Gene sequence:

>1245_bases
ATGAGTTATTTAGAAAAATTATTAAACGGTGTGGAAGTTGAATGGAAAACTGTAGATGATATTTTTTATATTAAAAATGG
TTATACGCCTTCTAAATCAAGTCAAGAATATTGGACTAACGGAACAAATCCTTGGTTTCGTATGGAAGATATTCGTAAAA
ATGGTAGAGTACTTTCGGATTCTATACAGCATGTTTCAGATTCAGCAGTTAAAGGACAACTCATTCCTGAAAACTCTTTA
TTAATGTCTACAACCGCAACAATAGGTGAACATGCATTAGTCTTGGTGCCTTACTTAACAAATCAACAAATAACAAACTT
TTCATTAAAAACATCATTCATAGATAAGGTCAGCATCAAGTATCTTTTTTATTGTTTTTTTGATTTTGGAAAATGGTGTA
TTGAGAATGCCAATAAAAATGGAGGCTTAAGTATTATTGGAACAAATAAGTTAAAAGAATATACTATCGCTATTCCATCT
TTAGAAATACAACAAAAAATAGTTGCAATTCTTGATTCATTTACGGAGCTTACAGCGGAGCTTACAGCGGAGCTTACAGC
GGAGCTTACAGCTCGTAAAATGCAATATAGTTATTATAGAGAGAAATTGTACACCTTTGATAAAAACAAAGTACAACATT
TACCAATGGATGATGAAAGTATTGGAGTGTTTCAAAGGGGTAAACGCTTTGTAAAAACTGATTTGATTTCTGAAGGAGTT
CCTGTTATTCATTATGGCGAAATGTATACACATTACGGAACTTGGGCAGATAAAACAAAATCCTTTTTGAGTGAAGAACT
AGTTAAAAATAAAAATCTACGGGTTGCCAATAAAGGTGATGTTGTCATAGTAGCAGCAGGTGAAACTATCGAAGATATCG
GAATGGGAACGGCTTGGTTAGGTGACGAAGGTGTAGTTGTTCATGATGCATGTTTTTCATATAAAACAACATTAAACCCA
AAGTTTGTGGCTTATTTTACACGCACAAAACAATTCCATGATCAAATTAAAAAACACATTTCTTCCGGTAAAATTTCTGC
TATCAATGCAAATGGTTTAGGTAAAGCAATAATTCCAGTTCCTTCAAAAGAAGAACAAGAACGTGTAGTTTCTATTCTAG
ACAAATTTGATGTTTTAACTAATTCTATAAGTGAAGGTCTGCCAAAAGAAATAGAATTAAGAAAAAAACAATACGAGTAT
TATAGAGACTTGTTACTAACATTTCCTAAGAACAATCTAGAATAA

Upstream 100 bases:

>100_bases
AAACTAACATAGTTGAATTGAACAAAGAAGTTACTAAAACAGTAATCAAAATAGATAAACTCCGTGCTGATATTGATACA
ATTATAAACGAAATTGAAGC

Downstream 100 bases:

>100_bases
TAATATGGCACAGTCATTAACTGAAATAGCACAAAAATTAAAAAATGCTGATAAAAAAGTAAAAGTACAATTGATTTATG
CTTTCAATGGCTCTGGAAAA

Product: restriction modification system DNA specificity subunit

Products: NA

Alternate protein names: S.EcoprrI; Type I restriction enzyme EcoprrI specificity protein; S protein [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MSYLEKLLNGVEVEWKTVDDIFYIKNGYTPSKSSQEYWTNGTNPWFRMEDIRKNGRVLSDSIQHVSDSAVKGQLIPENSL
LMSTTATIGEHALVLVPYLTNQQITNFSLKTSFIDKVSIKYLFYCFFDFGKWCIENANKNGGLSIIGTNKLKEYTIAIPS
LEIQQKIVAILDSFTELTAELTAELTAELTARKMQYSYYREKLYTFDKNKVQHLPMDDESIGVFQRGKRFVKTDLISEGV
PVIHYGEMYTHYGTWADKTKSFLSEELVKNKNLRVANKGDVVIVAAGETIEDIGMGTAWLGDEGVVVHDACFSYKTTLNP
KFVAYFTRTKQFHDQIKKHISSGKISAINANGLGKAIIPVPSKEEQERVVSILDKFDVLTNSISEGLPKEIELRKKQYEY
YRDLLLTFPKNNLE

Sequences:

>Translated_414_residues
MSYLEKLLNGVEVEWKTVDDIFYIKNGYTPSKSSQEYWTNGTNPWFRMEDIRKNGRVLSDSIQHVSDSAVKGQLIPENSL
LMSTTATIGEHALVLVPYLTNQQITNFSLKTSFIDKVSIKYLFYCFFDFGKWCIENANKNGGLSIIGTNKLKEYTIAIPS
LEIQQKIVAILDSFTELTAELTAELTAELTARKMQYSYYREKLYTFDKNKVQHLPMDDESIGVFQRGKRFVKTDLISEGV
PVIHYGEMYTHYGTWADKTKSFLSEELVKNKNLRVANKGDVVIVAAGETIEDIGMGTAWLGDEGVVVHDACFSYKTTLNP
KFVAYFTRTKQFHDQIKKHISSGKISAINANGLGKAIIPVPSKEEQERVVSILDKFDVLTNSISEGLPKEIELRKKQYEY
YRDLLLTFPKNNLE
>Mature_413_residues
SYLEKLLNGVEVEWKTVDDIFYIKNGYTPSKSSQEYWTNGTNPWFRMEDIRKNGRVLSDSIQHVSDSAVKGQLIPENSLL
MSTTATIGEHALVLVPYLTNQQITNFSLKTSFIDKVSIKYLFYCFFDFGKWCIENANKNGGLSIIGTNKLKEYTIAIPSL
EIQQKIVAILDSFTELTAELTAELTAELTARKMQYSYYREKLYTFDKNKVQHLPMDDESIGVFQRGKRFVKTDLISEGVP
VIHYGEMYTHYGTWADKTKSFLSEELVKNKNLRVANKGDVVIVAAGETIEDIGMGTAWLGDEGVVVHDACFSYKTTLNPK
FVAYFTRTKQFHDQIKKHISSGKISAINANGLGKAIIPVPSKEEQERVVSILDKFDVLTNSISEGLPKEIELRKKQYEYY
RDLLLTFPKNNLE

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 47144; Mature: 47013

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYLEKLLNGVEVEWKTVDDIFYIKNGYTPSKSSQEYWTNGTNPWFRMEDIRKNGRVLSD
CCHHHHHHCCCEEEEEEECEEEEEECCCCCCCCCCHHCCCCCCCCEEHHHHHHCCCCHHH
SIQHVSDSAVKGQLIPENSLLMSTTATIGEHALVLVPYLTNQQITNFSLKTSFIDKVSIK
HHHHHCCHHHCCEECCCCCEEEEEHHHCCCCEEEEEEEECCCCEECEEEHHHHHHHHHHH
YLFYCFFDFGKWCIENANKNGGLSIIGTNKLKEYTIAIPSLEIQQKIVAILDSFTELTAE
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
LTAELTAELTARKMQYSYYREKLYTFDKNKVQHLPMDDESIGVFQRGKRFVKTDLISEGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHCCHHHHHHHHHCCC
PVIHYGEMYTHYGTWADKTKSFLSEELVKNKNLRVANKGDVVIVAAGETIEDIGMGTAWL
CEEEECCHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCHHHHCCCEEEE
GDEGVVVHDACFSYKTTLNPKFVAYFTRTKQFHDQIKKHISSGKISAINANGLGKAIIPV
CCCCEEEEECHHCEECCCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECC
PSKEEQERVVSILDKFDVLTNSISEGLPKEIELRKKQYEYYRDLLLTFPKNNLE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SYLEKLLNGVEVEWKTVDDIFYIKNGYTPSKSSQEYWTNGTNPWFRMEDIRKNGRVLSD
CHHHHHHCCCEEEEEEECEEEEEECCCCCCCCCCHHCCCCCCCCEEHHHHHHCCCCHHH
SIQHVSDSAVKGQLIPENSLLMSTTATIGEHALVLVPYLTNQQITNFSLKTSFIDKVSIK
HHHHHCCHHHCCEECCCCCEEEEEHHHCCCCEEEEEEEECCCCEECEEEHHHHHHHHHHH
YLFYCFFDFGKWCIENANKNGGLSIIGTNKLKEYTIAIPSLEIQQKIVAILDSFTELTAE
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
LTAELTAELTARKMQYSYYREKLYTFDKNKVQHLPMDDESIGVFQRGKRFVKTDLISEGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHCCHHHHHHHHHCCC
PVIHYGEMYTHYGTWADKTKSFLSEELVKNKNLRVANKGDVVIVAAGETIEDIGMGTAWL
CEEEECCHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCHHHHCCCEEEE
GDEGVVVHDACFSYKTTLNPKFVAYFTRTKQFHDQIKKHISSGKISAINANGLGKAIIPV
CCCCEEEEECHHCEECCCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECC
PSKEEQERVVSILDKFDVLTNSISEGLPKEIELRKKQYEYYRDLLLTFPKNNLE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1691706 [H]