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The map label for this gene is hsdM [H]

Identifier: 146302128

GI number: 146302128

Start: 5297019

End: 5298566

Strand: Direct

Name: hsdM [H]

Synonym: Fjoh_4393

Alternate gene names: 146302128

Gene position: 5297019-5298566 (Clockwise)

Preceding gene: 146302127

Following gene: 146302129

Centisome position: 86.88

GC content: 34.3

Gene sequence:

>1548_bases
ATGACAAGCACCGCACAAAGAGCAGAATTACTAGCCAAAATTTGGAAAATAGCCAACGAGGTTCGTGGGGCTGTAGATGG
ATGGGATTTCAAACAATTTGTATTAGGAACACTTTTCTATCGCTACATTAGTGAGAACTTTACGAATTATATAGAGGCTG
GTGATGATAGCATTGATTACGCTAGCTTATCGGATGATGTAATTACACCAGAAATAAAAGATGATGCAATTAAAACAAAA
GGATATTTTATCTATCCTAGTCAACTTTATGTAAATATTGCCAAAACGGCTAATACCAATCCAAACCTGAACACCGATTT
AAAAAACATTTTTACAGCAATTGAAAGTTCTGCAAATGGGTATCCATCGGAAGAAGCTATAAAAGGTTTATTTGCTGATT
TTGACACTACAAGTTCACGATTAGGGAATACAGTTGAAAATAAAAACAGCCGTTTAGCATCGGTTTTAAAAGGAGTTGAA
GAACTAAATTTTGGTAATTTTGAAGACAATAAAATTGACCTTTTTGGGGATGCCTATGAAATTCTGATTTCAAATTATGC
AGCTAATGCGGGTAAATCGGGTGGTGAGTTTTTTACACCTGTGCACGTGTCAAAGCTAATTGCACAATTAGCTATGCACA
AACAAGAAAAAGTGAATAAAATTTATGATCCTGCAGCAGGTTCTGGATCTTTACTGCTGCAAGCCAAAAAACACTTCGAT
GACCATATCATTGAAGAAGGTTTCTTTGGACAAGAAATAAATCATACTACTTATAACTTGGCTCGTATGAACATGTTTTT
ACACAACGTCAACTACGACAAGTTTAATATAGCTTTAGGTGATACACTTCATCATCCTCACTATATTGATGACAAACCTT
TTGATGCTATTGTATCCAATCCTCCTTATTCGATAAAATGGATTGGAGATGATGACCCAACACTGATAAATGATGACCGT
TTTGCACCTGCTGGCGTACTAGCTCCTAAATCAAAAGCAGATTTTGCATTTGTTCTGCATGCACTAAGTTATCTTTCCAG
TAAAGGAAGGGCAGCTATAGTTTGTTTTCCTGGTATTTTTTACCGTGGTGGTGCGGAACAAAAAATTAGAAAGTATTTAG
TGGATAATAATTTTGTAGAAACTATCATCTCTGTAGCGCCTAATTTGTTTTATGGAACTTCTATAGCGGTTACAATTCTG
GTACTTTCAAAACATAAGACTGATACTACTACACAATTTATTGATGCAAGTGGTGAAGATTTCTTCAAGAAGGTTACCAA
CAACAATATGATGACCGATACACATATTGATAAAATAATGGAGTTATTTGATAGTAAGGTCGATGTAGAACATGTTGCAA
AATCAATCGACAATACTAAAATTGCTGAAAACGATTATAACCTATCAGTAAGTTCTTATGTAGAACCGAAAGACAATCGA
GTAAAAACTAACATAGTTGAATTGAACAAAGAAGTTACTAAAACAGTAATCAAAATAGATAAACTCCGTGCTGATATTGA
TACAATTATAAACGAAATTGAAGCATGA

Upstream 100 bases:

>100_bases
AAATAAAAGATTTACCCGATTTTGAGCAAGTGGAAAGAGAAGTGAGTAGAAAGATTAAAAACTTTATGGTTTAACCAACG
AAATAAACAAAAGAAAAATA

Downstream 100 bases:

>100_bases
GTTATTTAGAAAAATTATTAAACGGTGTGGAAGTTGAATGGAAAACTGTAGATGATATTTTTTATATTAAAAATGGTTAT
ACGCCTTCTAAATCAAGTCA

Product: type I restriction-modification system, M subunit

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MTSTAQRAELLAKIWKIANEVRGAVDGWDFKQFVLGTLFYRYISENFTNYIEAGDDSIDYASLSDDVITPEIKDDAIKTK
GYFIYPSQLYVNIAKTANTNPNLNTDLKNIFTAIESSANGYPSEEAIKGLFADFDTTSSRLGNTVENKNSRLASVLKGVE
ELNFGNFEDNKIDLFGDAYEILISNYAANAGKSGGEFFTPVHVSKLIAQLAMHKQEKVNKIYDPAAGSGSLLLQAKKHFD
DHIIEEGFFGQEINHTTYNLARMNMFLHNVNYDKFNIALGDTLHHPHYIDDKPFDAIVSNPPYSIKWIGDDDPTLINDDR
FAPAGVLAPKSKADFAFVLHALSYLSSKGRAAIVCFPGIFYRGGAEQKIRKYLVDNNFVETIISVAPNLFYGTSIAVTIL
VLSKHKTDTTTQFIDASGEDFFKKVTNNNMMTDTHIDKIMELFDSKVDVEHVAKSIDNTKIAENDYNLSVSSYVEPKDNR
VKTNIVELNKEVTKTVIKIDKLRADIDTIINEIEA

Sequences:

>Translated_515_residues
MTSTAQRAELLAKIWKIANEVRGAVDGWDFKQFVLGTLFYRYISENFTNYIEAGDDSIDYASLSDDVITPEIKDDAIKTK
GYFIYPSQLYVNIAKTANTNPNLNTDLKNIFTAIESSANGYPSEEAIKGLFADFDTTSSRLGNTVENKNSRLASVLKGVE
ELNFGNFEDNKIDLFGDAYEILISNYAANAGKSGGEFFTPVHVSKLIAQLAMHKQEKVNKIYDPAAGSGSLLLQAKKHFD
DHIIEEGFFGQEINHTTYNLARMNMFLHNVNYDKFNIALGDTLHHPHYIDDKPFDAIVSNPPYSIKWIGDDDPTLINDDR
FAPAGVLAPKSKADFAFVLHALSYLSSKGRAAIVCFPGIFYRGGAEQKIRKYLVDNNFVETIISVAPNLFYGTSIAVTIL
VLSKHKTDTTTQFIDASGEDFFKKVTNNNMMTDTHIDKIMELFDSKVDVEHVAKSIDNTKIAENDYNLSVSSYVEPKDNR
VKTNIVELNKEVTKTVIKIDKLRADIDTIINEIEA
>Mature_514_residues
TSTAQRAELLAKIWKIANEVRGAVDGWDFKQFVLGTLFYRYISENFTNYIEAGDDSIDYASLSDDVITPEIKDDAIKTKG
YFIYPSQLYVNIAKTANTNPNLNTDLKNIFTAIESSANGYPSEEAIKGLFADFDTTSSRLGNTVENKNSRLASVLKGVEE
LNFGNFEDNKIDLFGDAYEILISNYAANAGKSGGEFFTPVHVSKLIAQLAMHKQEKVNKIYDPAAGSGSLLLQAKKHFDD
HIIEEGFFGQEINHTTYNLARMNMFLHNVNYDKFNIALGDTLHHPHYIDDKPFDAIVSNPPYSIKWIGDDDPTLINDDRF
APAGVLAPKSKADFAFVLHALSYLSSKGRAAIVCFPGIFYRGGAEQKIRKYLVDNNFVETIISVAPNLFYGTSIAVTILV
LSKHKTDTTTQFIDASGEDFFKKVTNNNMMTDTHIDKIMELFDSKVDVEHVAKSIDNTKIAENDYNLSVSSYVEPKDNRV
KTNIVELNKEVTKTVIKIDKLRADIDTIINEIEA

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=250, Percent_Identity=28.4, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 57589; Mature: 57458

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTAQRAELLAKIWKIANEVRGAVDGWDFKQFVLGTLFYRYISENFTNYIEAGDDSIDY
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ASLSDDVITPEIKDDAIKTKGYFIYPSQLYVNIAKTANTNPNLNTDLKNIFTAIESSANG
CCCCCCCCCCCCCCCCEECCCEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
YPSEEAIKGLFADFDTTSSRLGNTVENKNSRLASVLKGVEELNFGNFEDNKIDLFGDAYE
CCCHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHH
ILISNYAANAGKSGGEFFTPVHVSKLIAQLAMHKQEKVNKIYDPAAGSGSLLLQAKKHFD
HHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHH
DHIIEEGFFGQEINHTTYNLARMNMFLHNVNYDKFNIALGDTLHHPHYIDDKPFDAIVSN
HHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHCC
PPYSIKWIGDDDPTLINDDRFAPAGVLAPKSKADFAFVLHALSYLSSKGRAAIVCFPGIF
CCEEEEEECCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCE
YRGGAEQKIRKYLVDNNFVETIISVAPNLFYGTSIAVTILVLSKHKTDTTTQFIDASGED
ECCCHHHHHHHHHHCCHHHHHHHHHCCCHHCCCHHHEEEEEEECCCCCCHHHHHCCCCHH
FFKKVTNNNMMTDTHIDKIMELFDSKVDVEHVAKSIDNTKIAENDYNLSVSSYVEPKDNR
HHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCEECCCCCCEEHHCCCCCCCCC
VKTNIVELNKEVTKTVIKIDKLRADIDTIINEIEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSTAQRAELLAKIWKIANEVRGAVDGWDFKQFVLGTLFYRYISENFTNYIEAGDDSIDY
CCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ASLSDDVITPEIKDDAIKTKGYFIYPSQLYVNIAKTANTNPNLNTDLKNIFTAIESSANG
CCCCCCCCCCCCCCCCEECCCEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
YPSEEAIKGLFADFDTTSSRLGNTVENKNSRLASVLKGVEELNFGNFEDNKIDLFGDAYE
CCCHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHH
ILISNYAANAGKSGGEFFTPVHVSKLIAQLAMHKQEKVNKIYDPAAGSGSLLLQAKKHFD
HHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHH
DHIIEEGFFGQEINHTTYNLARMNMFLHNVNYDKFNIALGDTLHHPHYIDDKPFDAIVSN
HHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHCC
PPYSIKWIGDDDPTLINDDRFAPAGVLAPKSKADFAFVLHALSYLSSKGRAAIVCFPGIF
CCEEEEEECCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCE
YRGGAEQKIRKYLVDNNFVETIISVAPNLFYGTSIAVTILVLSKHKTDTTTQFIDASGED
ECCCHHHHHHHHHHCCHHHHHHHHHCCCHHCCCHHHEEEEEEECCCCCCHHHHHCCCCHH
FFKKVTNNNMMTDTHIDKIMELFDSKVDVEHVAKSIDNTKIAENDYNLSVSSYVEPKDNR
HHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCEECCCCCCEEHHCCCCCCCCC
VKTNIVELNKEVTKTVIKIDKLRADIDTIINEIEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]