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The map label for this gene is gdhA [H]

Identifier: 146302070

GI number: 146302070

Start: 5238635

End: 5239882

Strand: Reverse

Name: gdhA [H]

Synonym: Fjoh_4334

Alternate gene names: 146302070

Gene position: 5239882-5238635 (Counterclockwise)

Preceding gene: 146302071

Following gene: 146302069

Centisome position: 85.94

GC content: 38.86

Gene sequence:

>1248_bases
ATGATTGACAGGTTTAATATAGCAGCCGATATTCTTAATTTAGACGAATCGATCAGACAAAAGCTGCAACGTCCTGAGAA
ACAAATTGTAGTAAATTTTTCGATTACATTAGATAACGGAACCGAACAAAACTTTGAAGGATACCGCGTAATTCACAATA
CAGCTTTAGGCCCTTCAAAAGGAGGTATACGTTATGATACTGCCGTAAACCTTGATGAAGTAAAAGCATTGGCCGCCTGG
ATGACCTGGAAATCTGCTGTAACCGGAATTCCCTTTGGAGGAGCAAAAGGCGGCATTATCTGCGATCCAAAAACACTTTC
TAAAACAGAATTAGAAAGAATTACAAGAGCTTATACAAAAGCGTTATCCGATATTTTTGGCCCTGAAAAAGATGTTCCTG
CGCCGGACATGGGGACAGGCCCGGACGAAATGGGCTGGTTAATGGATGAATTTTCATTGGTTCACGGAAAAACGATTCAT
GCTGTTGTAACTGGGAAACATCTGCATTCCGGAGGTTCGTTAGGCAGAGTAGAAGCAACAGGAAGAGGAGTAAGCATTAT
TACGCTGCTTGCACTTGAAAAATTAAAATTGAGACCGGCAAGATCTACGGCAGCAATTCAGGGATTTGGAAATGTTGGTC
TGCATTCTGCTTTGTTTTTATACGAAAAAGGAGTGAAGATTGTAGCCGTAAGCGATGTTTCAGAAGCTTTTTACAATCCG
GACGGGATTAATATTCCAGAGTTGATTTTGTATTACAATCTAAATAATAAAACCATAAAAGGTTATCCAAATTCAGTTGC
CATTAAACATGAAGATTTACTGCTTTTAGATGTTGATGTTTTAATTCCGGCTGCAAAAGAAGATGTAATTACACAGAAAA
ATGCCAAAGACATTCGCGCAAAAATAATTATCGAAGCAGCAAACGGTCCTGTTTCATCTGATGCCGATAAAATTCTGCAT
GAAAACAACGTTTTGGTCGTTCCGGATATTTTAGCAAATGCCGGCGGTGTTACGGTTTCTTATTTTGAGTGGCTTCAAAA
CTCATTATTAGAATCATGGAGAATTCACCAGATTAACAAACGTCTGGAAGATATTTTAGAAAAAGGTTTTGAAACCGTAT
TTAGAGTTGCAGTGAAACATGACGTTACGCCCAGAATTGCAGCGTATATAATCGCGCTGAAAAAAGTAGCCGAGACACAA
TCTGTAAAAGAAGTCGCTCTCGAAGCGCCAAAATTTAAACAGAATTAA

Upstream 100 bases:

>100_bases
ATTGCCGATAAACTTTATTAAACCCAAATTCTATAAACCCCAAAAATTTAAAATAAATACTATGAGTACAGAAATTATAA
AACAGAATCCCTTTCAATCG

Downstream 100 bases:

>100_bases
GTTTTTATAACCATAATCTAAGATGGGTTTCATCAGTCAATATCATGTCATAAAACAGCAGATATTGATTGATGAAATTG
TCTTTTTAATTCAATTAGTT

Product: Glu/Leu/Phe/Val dehydrogenase, C terminal

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MIDRFNIAADILNLDESIRQKLQRPEKQIVVNFSITLDNGTEQNFEGYRVIHNTALGPSKGGIRYDTAVNLDEVKALAAW
MTWKSAVTGIPFGGAKGGIICDPKTLSKTELERITRAYTKALSDIFGPEKDVPAPDMGTGPDEMGWLMDEFSLVHGKTIH
AVVTGKHLHSGGSLGRVEATGRGVSIITLLALEKLKLRPARSTAAIQGFGNVGLHSALFLYEKGVKIVAVSDVSEAFYNP
DGINIPELILYYNLNNKTIKGYPNSVAIKHEDLLLLDVDVLIPAAKEDVITQKNAKDIRAKIIIEAANGPVSSDADKILH
ENNVLVVPDILANAGGVTVSYFEWLQNSLLESWRIHQINKRLEDILEKGFETVFRVAVKHDVTPRIAAYIIALKKVAETQ
SVKEVALEAPKFKQN

Sequences:

>Translated_415_residues
MIDRFNIAADILNLDESIRQKLQRPEKQIVVNFSITLDNGTEQNFEGYRVIHNTALGPSKGGIRYDTAVNLDEVKALAAW
MTWKSAVTGIPFGGAKGGIICDPKTLSKTELERITRAYTKALSDIFGPEKDVPAPDMGTGPDEMGWLMDEFSLVHGKTIH
AVVTGKHLHSGGSLGRVEATGRGVSIITLLALEKLKLRPARSTAAIQGFGNVGLHSALFLYEKGVKIVAVSDVSEAFYNP
DGINIPELILYYNLNNKTIKGYPNSVAIKHEDLLLLDVDVLIPAAKEDVITQKNAKDIRAKIIIEAANGPVSSDADKILH
ENNVLVVPDILANAGGVTVSYFEWLQNSLLESWRIHQINKRLEDILEKGFETVFRVAVKHDVTPRIAAYIIALKKVAETQ
SVKEVALEAPKFKQN
>Mature_415_residues
MIDRFNIAADILNLDESIRQKLQRPEKQIVVNFSITLDNGTEQNFEGYRVIHNTALGPSKGGIRYDTAVNLDEVKALAAW
MTWKSAVTGIPFGGAKGGIICDPKTLSKTELERITRAYTKALSDIFGPEKDVPAPDMGTGPDEMGWLMDEFSLVHGKTIH
AVVTGKHLHSGGSLGRVEATGRGVSIITLLALEKLKLRPARSTAAIQGFGNVGLHSALFLYEKGVKIVAVSDVSEAFYNP
DGINIPELILYYNLNNKTIKGYPNSVAIKHEDLLLLDVDVLIPAAKEDVITQKNAKDIRAKIIIEAANGPVSSDADKILH
ENNVLVVPDILANAGGVTVSYFEWLQNSLLESWRIHQINKRLEDILEKGFETVFRVAVKHDVTPRIAAYIIALKKVAETQ
SVKEVALEAPKFKQN

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=359, Percent_Identity=44.5682451253482, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI4885281, Length=332, Percent_Identity=45.7831325301205, Blast_Score=281, Evalue=1e-75,
Organism=Escherichia coli, GI1788059, Length=359, Percent_Identity=30.9192200557103, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17544676, Length=353, Percent_Identity=44.4759206798867, Blast_Score=269, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6324951, Length=388, Percent_Identity=30.9278350515464, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6319256, Length=391, Percent_Identity=30.9462915601023, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24649283, Length=350, Percent_Identity=46.8571428571429, Blast_Score=281, Evalue=6e-76,
Organism=Drosophila melanogaster, GI45549226, Length=340, Percent_Identity=45.8823529411765, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI45553475, Length=384, Percent_Identity=43.4895833333333, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24649460, Length=280, Percent_Identity=46.7857142857143, Blast_Score=234, Evalue=6e-62,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 45523; Mature: 45523

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRFNIAADILNLDESIRQKLQRPEKQIVVNFSITLDNGTEQNFEGYRVIHNTALGPSK
CCCCHHHHHHHHHCCHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCEEEEEECCCCCCC
GGIRYDTAVNLDEVKALAAWMTWKSAVTGIPFGGAKGGIICDPKTLSKTELERITRAYTK
CCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHH
ALSDIFGPEKDVPAPDMGTGPDEMGWLMDEFSLVHGKTIHAVVTGKHLHSGGSLGRVEAT
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCEECCCCCCCEEEEC
GRGVSIITLLALEKLKLRPARSTAAIQGFGNVGLHSALFLYEKGVKIVAVSDVSEAFYNP
CCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHCCCEEEEEECHHHHHCCC
DGINIPELILYYNLNNKTIKGYPNSVAIKHEDLLLLDVDVLIPAAKEDVITQKNAKDIRA
CCCCCCEEEEEEECCCCEEECCCCCEEEEECCEEEEEEHEECCCCHHHHHCCCCCCCEEE
KIIIEAANGPVSSDADKILHENNVLVVPDILANAGGVTVSYFEWLQNSLLESWRIHQINK
EEEEEECCCCCCCCHHHHHHCCCEEEECHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RLEDILEKGFETVFRVAVKHDVTPRIAAYIIALKKVAETQSVKEVALEAPKFKQN
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIDRFNIAADILNLDESIRQKLQRPEKQIVVNFSITLDNGTEQNFEGYRVIHNTALGPSK
CCCCHHHHHHHHHCCHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCEEEEEECCCCCCC
GGIRYDTAVNLDEVKALAAWMTWKSAVTGIPFGGAKGGIICDPKTLSKTELERITRAYTK
CCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHH
ALSDIFGPEKDVPAPDMGTGPDEMGWLMDEFSLVHGKTIHAVVTGKHLHSGGSLGRVEAT
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCEECCCCCCCEEEEC
GRGVSIITLLALEKLKLRPARSTAAIQGFGNVGLHSALFLYEKGVKIVAVSDVSEAFYNP
CCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHCCCEEEEEECHHHHHCCC
DGINIPELILYYNLNNKTIKGYPNSVAIKHEDLLLLDVDVLIPAAKEDVITQKNAKDIRA
CCCCCCEEEEEEECCCCEEECCCCCEEEEECCEEEEEEHEECCCCHHHHHCCCCCCCEEE
KIIIEAANGPVSSDADKILHENNVLVVPDILANAGGVTVSYFEWLQNSLLESWRIHQINK
EEEEEECCCCCCCCHHHHHHCCCEEEECHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RLEDILEKGFETVFRVAVKHDVTPRIAAYIIALKKVAETQSVKEVALEAPKFKQN
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]