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The map label for this gene is acrE [H]

Identifier: 146302035

GI number: 146302035

Start: 5194669

End: 5195886

Strand: Direct

Name: acrE [H]

Synonym: Fjoh_4299

Alternate gene names: 146302035

Gene position: 5194669-5195886 (Clockwise)

Preceding gene: 146302034

Following gene: 146302036

Centisome position: 85.2

GC content: 37.68

Gene sequence:

>1218_bases
ATGAATCCCGAGAATGCCAGAATACCAAAGAATTTTATCCGAGAAAATGTTCAACCAATTAAAACCACTATGAAAATGAA
AAATGTAATTATAACCAGTTTTATTCTGGCCTTAGTATTAAGCAGCTGTGGCGACAAAAATCAAGCGCCTACTGCCCCGC
CGCCACCGGTTTTACCAGTTTTAGCTATCACAAGCGCAAACACAACTACTGATGCTGAATATCCAGCATCTATACAAGGA
ACCGTTGATGTTGAAATTCGCCCGCAGGTAAGCGGAAACCTTGACAGAATTTTTGTTGACGAAGGTGCTTATGTAAGTAA
AGGACAAACTTTATTTAAAATCAATGAACGTCCGTTTCGTGAGCAGTTAAACAATGCTTTAGCAAGTCTACACGCAGCAG
AAGCAGCTTTAATCAACGCTAATTTAGAAGTTGATAAACTGACTCCGCTAGTGCAAAACAAAGTAGTTTCTGATTATCAG
TTAAAAACAGCTAAAGCTTCTCAAAAAATTGCTGCTGCTAATATCGAGCAGGCAAAAGCAATGGTAGGTTCTGCTAAAAT
TAATTTAGGATATACTAACGTAACAGCTCCAGTGAGCGGATACATTGGAAGATTACCTAAAAAACAAGGAAGTTTAGTTT
CTGCATCTGATGTTGAAGCACTTACTACTTTATCTGATGTTCACGAAGTATTTGCTTATTTCTCTTTAGGCGAAACTGAT
TTCATCAACTTTAAAGAGCAATACAAAGGAAGTTCTCTTGGTGATAAAATTAAAAAATTACCTCCAGTTACTTTGATCTT
GGCTGATAACAACGCTTATCCGCAAACAGGAAAAATCGATATGGTTGATGGTCAGTTTGATAAAACTACAGGAGCAATCA
CGATTAGAGCAACATTCCCAAATGCAAACGGAACATTACGTTCTGGAAACACAGGAAGAATCCGTTTAGGATTACAGCAC
GACGATGCGATTTTAGTACCACAGTCGGCTACAGTTGAAATGCAGGACAAAGTATTTGTTTTCACTGTAGGCAAAGACAA
CAAAGTAACCAAAATGCCTATCACAGTTGTGGGTAAAAGCGGTACCAATTATTTAATTAAAGAAGGTGTAAAAACGGGTG
ACCAAATCGTGTTAAGCGGTATTGACAAACTTCAGGATGGTCAAGCGATTCAGCCTGAAAAATCAACTAAAGTTGCCGAA
GTAACTAATAAAAAATAA

Upstream 100 bases:

>100_bases
GATAATTTAATACTGATTCGAAATAGAATTGGAAAGGCTTACTGTATGGAAATTTGTGCACTTTTACTTAACAACGTTAG
ATAATTTAATTCAATTAAAT

Downstream 100 bases:

>100_bases
TTCTAAAACACAATGTTCAAAATATTTATACAAAGACCTGTACTGGCAACCGTAATTTCCATTTTATTGGTAATTCTGGG
GGTATTGGGTTTAACTAAAC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: Protein envC [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MNPENARIPKNFIRENVQPIKTTMKMKNVIITSFILALVLSSCGDKNQAPTAPPPPVLPVLAITSANTTTDAEYPASIQG
TVDVEIRPQVSGNLDRIFVDEGAYVSKGQTLFKINERPFREQLNNALASLHAAEAALINANLEVDKLTPLVQNKVVSDYQ
LKTAKASQKIAAANIEQAKAMVGSAKINLGYTNVTAPVSGYIGRLPKKQGSLVSASDVEALTTLSDVHEVFAYFSLGETD
FINFKEQYKGSSLGDKIKKLPPVTLILADNNAYPQTGKIDMVDGQFDKTTGAITIRATFPNANGTLRSGNTGRIRLGLQH
DDAILVPQSATVEMQDKVFVFTVGKDNKVTKMPITVVGKSGTNYLIKEGVKTGDQIVLSGIDKLQDGQAIQPEKSTKVAE
VTNKK

Sequences:

>Translated_405_residues
MNPENARIPKNFIRENVQPIKTTMKMKNVIITSFILALVLSSCGDKNQAPTAPPPPVLPVLAITSANTTTDAEYPASIQG
TVDVEIRPQVSGNLDRIFVDEGAYVSKGQTLFKINERPFREQLNNALASLHAAEAALINANLEVDKLTPLVQNKVVSDYQ
LKTAKASQKIAAANIEQAKAMVGSAKINLGYTNVTAPVSGYIGRLPKKQGSLVSASDVEALTTLSDVHEVFAYFSLGETD
FINFKEQYKGSSLGDKIKKLPPVTLILADNNAYPQTGKIDMVDGQFDKTTGAITIRATFPNANGTLRSGNTGRIRLGLQH
DDAILVPQSATVEMQDKVFVFTVGKDNKVTKMPITVVGKSGTNYLIKEGVKTGDQIVLSGIDKLQDGQAIQPEKSTKVAE
VTNKK
>Mature_405_residues
MNPENARIPKNFIRENVQPIKTTMKMKNVIITSFILALVLSSCGDKNQAPTAPPPPVLPVLAITSANTTTDAEYPASIQG
TVDVEIRPQVSGNLDRIFVDEGAYVSKGQTLFKINERPFREQLNNALASLHAAEAALINANLEVDKLTPLVQNKVVSDYQ
LKTAKASQKIAAANIEQAKAMVGSAKINLGYTNVTAPVSGYIGRLPKKQGSLVSASDVEALTTLSDVHEVFAYFSLGETD
FINFKEQYKGSSLGDKIKKLPPVTLILADNNAYPQTGKIDMVDGQFDKTTGAITIRATFPNANGTLRSGNTGRIRLGLQH
DDAILVPQSATVEMQDKVFVFTVGKDNKVTKMPITVVGKSGTNYLIKEGVKTGDQIVLSGIDKLQDGQAIQPEKSTKVAE
VTNKK

Specific function: May affect specific membrane functions, such as septum formation during cell division, and cell membrane permeability [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=375, Percent_Identity=32.8, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI1789929, Length=366, Percent_Identity=29.5081967213115, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1786668, Length=363, Percent_Identity=32.2314049586777, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI87082036, Length=311, Percent_Identity=26.0450160771704, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI87081796, Length=377, Percent_Identity=24.4031830238727, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87081951, Length=163, Percent_Identity=31.2883435582822, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43656; Mature: 43656

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPENARIPKNFIRENVQPIKTTMKMKNVIITSFILALVLSSCGDKNQAPTAPPPPVLPV
CCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
LAITSANTTTDAEYPASIQGTVDVEIRPQVSGNLDRIFVDEGAYVSKGQTLFKINERPFR
EEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCEEECCCEEEEECCCHHH
EQLNNALASLHAAEAALINANLEVDKLTPLVQNKVVSDYQLKTAKASQKIAAANIEQAKA
HHHHHHHHHHHHHHEEEEECCCCHHHHCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHH
MVGSAKINLGYTNVTAPVSGYIGRLPKKQGSLVSASDVEALTTLSDVHEVFAYFSLGETD
HHCCEEEEECCCCCCCCHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCC
FINFKEQYKGSSLGDKIKKLPPVTLILADNNAYPQTGKIDMVDGQFDKTTGAITIRATFP
EECCHHHCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCEEEEEEECC
NANGTLRSGNTGRIRLGLQHDDAILVPQSATVEMQDKVFVFTVGKDNKVTKMPITVVGKS
CCCCEEECCCCCEEEEEEECCCEEEECCCCCEEEECEEEEEEECCCCCEEEEEEEEEECC
GTNYLIKEGVKTGDQIVLSGIDKLQDGQAIQPEKSTKVAEVTNKK
CCCHHHHCCCCCCCCEEEHHHHHHCCCCCCCCCCCCEEHHCCCCC
>Mature Secondary Structure
MNPENARIPKNFIRENVQPIKTTMKMKNVIITSFILALVLSSCGDKNQAPTAPPPPVLPV
CCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
LAITSANTTTDAEYPASIQGTVDVEIRPQVSGNLDRIFVDEGAYVSKGQTLFKINERPFR
EEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCEEECCCEEEEECCCHHH
EQLNNALASLHAAEAALINANLEVDKLTPLVQNKVVSDYQLKTAKASQKIAAANIEQAKA
HHHHHHHHHHHHHHEEEEECCCCHHHHCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHH
MVGSAKINLGYTNVTAPVSGYIGRLPKKQGSLVSASDVEALTTLSDVHEVFAYFSLGETD
HHCCEEEEECCCCCCCCHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCC
FINFKEQYKGSSLGDKIKKLPPVTLILADNNAYPQTGKIDMVDGQFDKTTGAITIRATFP
EECCHHHCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCEEEEEEECC
NANGTLRSGNTGRIRLGLQHDDAILVPQSATVEMQDKVFVFTVGKDNKVTKMPITVVGKS
CCCCEEECCCCCEEEEEEECCCEEEECCCCCEEEECEEEEEEECCCCCEEEEEEEEEECC
GTNYLIKEGVKTGDQIVLSGIDKLQDGQAIQPEKSTKVAEVTNKK
CCCHHHHCCCCCCCCEEEHHHHHHCCCCCCCCCCCCEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1720861; 9278503 [H]