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The map label for this gene is nolF [H]

Identifier: 146302022

GI number: 146302022

Start: 5178967

End: 5180052

Strand: Direct

Name: nolF [H]

Synonym: Fjoh_4286

Alternate gene names: 146302022

Gene position: 5178967-5180052 (Clockwise)

Preceding gene: 146302021

Following gene: 146302023

Centisome position: 84.94

GC content: 36.46

Gene sequence:

>1086_bases
ATGAAAAATAACATTATAAAATCTACAGCGATACTATTTACAGCTTTAGTTGTACTAAGCTGTGAAAAGAAAAAAGAAGA
AACTGCGAAAGCAGAAATCGAACCAAAAGTTGAAACTTTCCTTTTATCGAAAGAAAAACTAACAACAGAATTGCGTTTGC
CAGCTGAATTAACCGGTTTTCAACAAGTTGATTTGTATGCAAAAGTGAGCAGTTTCGTAAAAACCTTAAAAGTTGATATT
GGTACGAAAGTAAAAAAAGGACAGCTTTTAATTGTTTTAGAAGCACCAGAAATCAGTTCGCAATTGGCTGCAGCCGAATC
TAGACTAAAATCTATGGAAGCGATTTACGCAACCAGCAAAAGTACTTACAACCGTTTGTATGAAACAAGCAAGGTTGAAG
GAACGATTTCTAAAAACGATTTAGAAATGGCAAGCGGAAAAAAGAATTCTGATTACGCACAATATCAGGCAGCAATTGCA
GCACACAAAGAAATCTCGATTATGAGAGGCTACTTAGAAATTCGTGCTCCTTTTGACGGTGTTGTAGCAGCTAGAAATGT
CAATTTAGGAACATTTGTAGGCCCGGCAGGAAAAGGATCTGATTTGCCTTTATTAACGATTCAGGAGCAAAGCAAATTAC
GTTTGGCGGTTTCTGTTCCAGAATTGTACACAGGATATTTGCACCCAGGTGACGAAATGAGTTTTAATGTAAAATCGCTA
CCAGAAACTTTTACAGCTAAAATTACCAGAATGTCTGGTGCTTTAGATTTAAAATTACGTTCTGAAAGAGTCGAAATGGA
CGTTCACAACACAAAAGGAAATTTATTACCTGGAATGGTTGCCGAAGTTCTTTTACCGCTTAACGCGAAAGACAGCACTT
ATGTAGTGCCAAAAACTGCAGTAGTGAATTCTGCAGAAGGTTTATATGTAGTGAAAGTAGTAAACCACAAAGCGACAAGA
GTTGACATTAAAAAAGGAAGAGAAATCGAAGATAAAATTGAAATTTTCGGCGATTTGACTCCAAACGATAAACTGGTAAA
AATTGCCAGCGAAGAAACTAAGGAAGGCGATGTCATAAACGAATAA

Upstream 100 bases:

>100_bases
AAAGTCGAAAGTCAAAAGTCGAAAGTTACGATCGTCGCGAAATACTTCTATAAACTTTTATACTTTCTGAATACTTATAA
AACATATATAAAACCAAAAA

Downstream 100 bases:

>100_bases
CCTGCGTTTAGTCTTCTTTTTAGTAGATTACCAATTACTGTACGCATTCTTAGGAGTGCAAATTTAATTTGCCTAAAGGG
CAGTTCCTCTTTAATTGGAG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKNNIIKSTAILFTALVVLSCEKKKEETAKAEIEPKVETFLLSKEKLTTELRLPAELTGFQQVDLYAKVSSFVKTLKVDI
GTKVKKGQLLIVLEAPEISSQLAAAESRLKSMEAIYATSKSTYNRLYETSKVEGTISKNDLEMASGKKNSDYAQYQAAIA
AHKEISIMRGYLEIRAPFDGVVAARNVNLGTFVGPAGKGSDLPLLTIQEQSKLRLAVSVPELYTGYLHPGDEMSFNVKSL
PETFTAKITRMSGALDLKLRSERVEMDVHNTKGNLLPGMVAEVLLPLNAKDSTYVVPKTAVVNSAEGLYVVKVVNHKATR
VDIKKGREIEDKIEIFGDLTPNDKLVKIASEETKEGDVINE

Sequences:

>Translated_361_residues
MKNNIIKSTAILFTALVVLSCEKKKEETAKAEIEPKVETFLLSKEKLTTELRLPAELTGFQQVDLYAKVSSFVKTLKVDI
GTKVKKGQLLIVLEAPEISSQLAAAESRLKSMEAIYATSKSTYNRLYETSKVEGTISKNDLEMASGKKNSDYAQYQAAIA
AHKEISIMRGYLEIRAPFDGVVAARNVNLGTFVGPAGKGSDLPLLTIQEQSKLRLAVSVPELYTGYLHPGDEMSFNVKSL
PETFTAKITRMSGALDLKLRSERVEMDVHNTKGNLLPGMVAEVLLPLNAKDSTYVVPKTAVVNSAEGLYVVKVVNHKATR
VDIKKGREIEDKIEIFGDLTPNDKLVKIASEETKEGDVINE
>Mature_361_residues
MKNNIIKSTAILFTALVVLSCEKKKEETAKAEIEPKVETFLLSKEKLTTELRLPAELTGFQQVDLYAKVSSFVKTLKVDI
GTKVKKGQLLIVLEAPEISSQLAAAESRLKSMEAIYATSKSTYNRLYETSKVEGTISKNDLEMASGKKNSDYAQYQAAIA
AHKEISIMRGYLEIRAPFDGVVAARNVNLGTFVGPAGKGSDLPLLTIQEQSKLRLAVSVPELYTGYLHPGDEMSFNVKSL
PETFTAKITRMSGALDLKLRSERVEMDVHNTKGNLLPGMVAEVLLPLNAKDSTYVVPKTAVVNSAEGLYVVKVVNHKATR
VDIKKGREIEDKIEIFGDLTPNDKLVKIASEETKEGDVINE

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=347, Percent_Identity=25.3602305475504, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789929, Length=357, Percent_Identity=21.8487394957983, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39668; Mature: 39668

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNIIKSTAILFTALVVLSCEKKKEETAKAEIEPKVETFLLSKEKLTTELRLPAELTGF
CCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCHHHEEECHHHHHHEEECCHHHCCC
QQVDLYAKVSSFVKTLKVDIGTKVKKGQLLIVLEAPEISSQLAAAESRLKSMEAIYATSK
HHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCH
STYNRLYETSKVEGTISKNDLEMASGKKNSDYAQYQAAIAAHKEISIMRGYLEIRAPFDG
HHHHHHHHHCCCCCEECCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCC
VVAARNVNLGTFVGPAGKGSDLPLLTIQEQSKLRLAVSVPELYTGYLHPGDEMSFNVKSL
EEEEECCCCCEEECCCCCCCCCEEEEECCCCCEEEEEECHHHHCCCCCCCCCCEEEHHHC
PETFTAKITRMSGALDLKLRSERVEMDVHNTKGNLLPGMVAEVLLPLNAKDSTYVVPKTA
CHHHHHHHHHCCCEEEEEEECCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCEEEEECHH
VVNSAEGLYVVKVVNHKATRVDIKKGREIEDKIEIFGDLTPNDKLVKIASEETKEGDVIN
EECCCCCEEEEEEECCCCEEEECCCCCCHHHHHHEEECCCCCCCEEEEECCCCCCCCCCC
E
C
>Mature Secondary Structure
MKNNIIKSTAILFTALVVLSCEKKKEETAKAEIEPKVETFLLSKEKLTTELRLPAELTGF
CCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCHHHEEECHHHHHHEEECCHHHCCC
QQVDLYAKVSSFVKTLKVDIGTKVKKGQLLIVLEAPEISSQLAAAESRLKSMEAIYATSK
HHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCH
STYNRLYETSKVEGTISKNDLEMASGKKNSDYAQYQAAIAAHKEISIMRGYLEIRAPFDG
HHHHHHHHHCCCCCEECCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCC
VVAARNVNLGTFVGPAGKGSDLPLLTIQEQSKLRLAVSVPELYTGYLHPGDEMSFNVKSL
EEEEECCCCCEEECCCCCCCCCEEEEECCCCCEEEEEECHHHHCCCCCCCCCCEEEHHHC
PETFTAKITRMSGALDLKLRSERVEMDVHNTKGNLLPGMVAEVLLPLNAKDSTYVVPKTA
CHHHHHHHHHCCCEEEEEEECCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCEEEEECHH
VVNSAEGLYVVKVVNHKATRVDIKKGREIEDKIEIFGDLTPNDKLVKIASEETKEGDVIN
EECCCCCEEEEEEECCCCEEEECCCCCCHHHHHHEEECCCCCCCEEEEECCCCCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]