Click here to switch to the map view.

The map label for this gene is kdgA [H]

Identifier: 146301996

GI number: 146301996

Start: 5146425

End: 5147093

Strand: Reverse

Name: kdgA [H]

Synonym: Fjoh_4260

Alternate gene names: 146301996

Gene position: 5147093-5146425 (Counterclockwise)

Preceding gene: 146301997

Following gene: 146301995

Centisome position: 84.42

GC content: 37.37

Gene sequence:

>669_bases
ATGGCAAAATATTCAAGAATAGAGGTTGCGCAGACAATGAAAGATAACGGAATGGTGCCGTTGTTTTTTCATTCTGATAT
TGAATTAAGTAAAAAAGTTTTAAAAGCTTGTTATGACGGTGGTTCCCGATTAATGGAATTTACCAGCAGAGGTGATTTTG
CACATGAAGTTTTTGGAGCTTTAAATAAATACGCTTTAGCAGAACTTCCGGGAATGATCTTAGGAGTTGGTTCTGTTACA
GATGCAGCTTCGGCGTCATTATACATGAGTTTAGGAGCTAATTTTATTGTAACTCCAGTATTTAGAGAAGACATTGCAAT
TGCCTGCAACCGCCGAAAAGTATTATGGTCTCCTGGATGTGGTACGCTTAGCGAAATTGCAAGAGCGGAAGAATTAGGCT
GTGAAATTGTAAAATTATTTCCGGCAGATACTTACGGACCAGAATTTATAAAAGCCATAAAAGGACCATGCCCGTGGACA
AACATTATGCCTACAGGCGGTGTTTACCCAACTGTTGAAAGTTTGAGTTCATGGCTTAACGCAGGGGCAACCTGTGTTGG
TTTAGGTTCACAATTAATTTCAAAAGATATATTAGACAAAAAAGACTTTGACGGTTTAACAGCTAAAGTAAGTCAGGTTC
TTGACATCATAAAAAATATAAGAAAATAA

Upstream 100 bases:

>100_bases
TACAATTGCAGGCGACTATAATCTGGTTACTTTAAAAGAAGTTGAAAATATGATTGATGGTGATGGTTCTGCATTAGTAT
CAAGATAATTAATTAAAAAA

Downstream 100 bases:

>100_bases
ATAAGGAGTAATCATGCCAACCCTTATTTATATGCGTTTTTTTTAGATTTTAGAGATTGTAATTGAGCATTACGCTTGCA
AAATAATTCCTGTCACTCCT

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MAKYSRIEVAQTMKDNGMVPLFFHSDIELSKKVLKACYDGGSRLMEFTSRGDFAHEVFGALNKYALAELPGMILGVGSVT
DAASASLYMSLGANFIVTPVFREDIAIACNRRKVLWSPGCGTLSEIARAEELGCEIVKLFPADTYGPEFIKAIKGPCPWT
NIMPTGGVYPTVESLSSWLNAGATCVGLGSQLISKDILDKKDFDGLTAKVSQVLDIIKNIRK

Sequences:

>Translated_222_residues
MAKYSRIEVAQTMKDNGMVPLFFHSDIELSKKVLKACYDGGSRLMEFTSRGDFAHEVFGALNKYALAELPGMILGVGSVT
DAASASLYMSLGANFIVTPVFREDIAIACNRRKVLWSPGCGTLSEIARAEELGCEIVKLFPADTYGPEFIKAIKGPCPWT
NIMPTGGVYPTVESLSSWLNAGATCVGLGSQLISKDILDKKDFDGLTAKVSQVLDIIKNIRK
>Mature_221_residues
AKYSRIEVAQTMKDNGMVPLFFHSDIELSKKVLKACYDGGSRLMEFTSRGDFAHEVFGALNKYALAELPGMILGVGSVTD
AASASLYMSLGANFIVTPVFREDIAIACNRRKVLWSPGCGTLSEIARAEELGCEIVKLFPADTYGPEFIKAIKGPCPWTN
IMPTGGVYPTVESLSSWLNAGATCVGLGSQLISKDILDKKDFDGLTAKVSQVLDIIKNIRK

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=183, Percent_Identity=30.0546448087432, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI48994954, Length=125, Percent_Identity=34.4, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 24053; Mature: 23922

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYSRIEVAQTMKDNGMVPLFFHSDIELSKKVLKACYDGGSRLMEFTSRGDFAHEVFGA
CCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHH
LNKYALAELPGMILGVGSVTDAASASLYMSLGANFIVTPVFREDIAIACNRRKVLWSPGC
HHHHHHHHCCHHEEECCCCCHHHHHHHHHHHCCCEEECHHHHHHHHHEECCCEEEECCCC
GTLSEIARAEELGCEIVKLFPADTYGPEFIKAIKGPCPWTNIMPTGGVYPTVESLSSWLN
CHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
AGATCVGLGSQLISKDILDKKDFDGLTAKVSQVLDIIKNIRK
CCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKYSRIEVAQTMKDNGMVPLFFHSDIELSKKVLKACYDGGSRLMEFTSRGDFAHEVFGA
CCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHH
LNKYALAELPGMILGVGSVTDAASASLYMSLGANFIVTPVFREDIAIACNRRKVLWSPGC
HHHHHHHHCCHHEEECCCCCHHHHHHHHHHHCCCEEECHHHHHHHHHEECCCEEEECCCC
GTLSEIARAEELGCEIVKLFPADTYGPEFIKAIKGPCPWTNIMPTGGVYPTVESLSSWLN
CHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
AGATCVGLGSQLISKDILDKKDFDGLTAKVSQVLDIIKNIRK
CCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]