| Definition | Flavobacterium johnsoniae UW101 chromosome, complete genome. |
|---|---|
| Accession | NC_009441 |
| Length | 6,096,872 |
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The map label for this gene is uxuB [H]
Identifier: 146301926
GI number: 146301926
Start: 5030678
End: 5031490
Strand: Direct
Name: uxuB [H]
Synonym: Fjoh_4190
Alternate gene names: 146301926
Gene position: 5030678-5031490 (Clockwise)
Preceding gene: 146301925
Following gene: 146301927
Centisome position: 82.51
GC content: 37.52
Gene sequence:
>813_bases ATGGATTCAATATTCAATTTAAAAGGAAAAATTGCTCTGATTACGGGAGGTGCCGGAGTTTTAGGAAGCAATTTTGCAAA TGTATTAGCAAAACAAGGTGTTATTGTGGGAATCGTTTCGCAGTCACTTGAAAAAGCCGAAAGCACTGTAAAAACTCTTG AAGCAAACGGCGGACAAGGTTTTGCGGTTCAGGCCAATGTTTTAAATAAAGAAGAAGTAGAAAAAGTAAGAGATTTTATT GTTGAAAAATACGGACGTCTTGATATTTTGATTAACGCAGCCGGCGGAAATATGCCCGGAGCGACTATCCAGCCAGATCA GGCAGTTTATGATATGAAAAGCGATGATTTACAAAAAGTAATCGATTTGAATATCATTGGAACCATGCTTCCTACTCAGG TTTTTGCAGAGTTATTTGCTAAACAAAAACAAGGAAATATCATTAATATTTCATCTGCTTCGGCACAAAGACCTTTAACG AGAGTTGTAGGTTATTCGGCTTCAAAAGCGGCGATTGATAATTTCACGCAATGGATGGCAGTTGAATTAGCCCAAAAATA TGGTGAAGGAATTCGTGTAAACGCTATTTCACCAGGTTTTTTCATTGGAGAACAAAATCGTGCTTTATTATTAACTCCGG AAGGAAAATTAACTCCAAGAGGTGAAAAAATCATCGATCACACGCCAATGGGAAGATTTGGAACTCCAGAAGATATTGAT GGTGCACTTTTGTTTTTATGCAGCGATATGTCAAAATTCGTAACAGGAACTATCTTAAAAGTTGACGGCGGTTTTGCTGC AACAAGTATCTAA
Upstream 100 bases:
>100_bases TTTTAAAGTAAAAATTTAAATAGTAAACTAAACCATAACCATACTTTATTTTCCAAATTGGAATTTGGGATTTTAAAGTA TTGAAAATTTAAACTAAAAA
Downstream 100 bases:
>100_bases AAACTAACTCACAAAACAACATAATACAAAATGGAACAAACATTAAGATGGTTCGGACCAAATGATCCTGTTTCTTTACA AGATATTGCACAAACCGGAG
Product: D-mannonate oxidoreductase
Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]
Alternate protein names: NA
Number of amino acids: Translated: 270; Mature: 270
Protein sequence:
>270_residues MDSIFNLKGKIALITGGAGVLGSNFANVLAKQGVIVGIVSQSLEKAESTVKTLEANGGQGFAVQANVLNKEEVEKVRDFI VEKYGRLDILINAAGGNMPGATIQPDQAVYDMKSDDLQKVIDLNIIGTMLPTQVFAELFAKQKQGNIINISSASAQRPLT RVVGYSASKAAIDNFTQWMAVELAQKYGEGIRVNAISPGFFIGEQNRALLLTPEGKLTPRGEKIIDHTPMGRFGTPEDID GALLFLCSDMSKFVTGTILKVDGGFAATSI
Sequences:
>Translated_270_residues MDSIFNLKGKIALITGGAGVLGSNFANVLAKQGVIVGIVSQSLEKAESTVKTLEANGGQGFAVQANVLNKEEVEKVRDFI VEKYGRLDILINAAGGNMPGATIQPDQAVYDMKSDDLQKVIDLNIIGTMLPTQVFAELFAKQKQGNIINISSASAQRPLT RVVGYSASKAAIDNFTQWMAVELAQKYGEGIRVNAISPGFFIGEQNRALLLTPEGKLTPRGEKIIDHTPMGRFGTPEDID GALLFLCSDMSKFVTGTILKVDGGFAATSI >Mature_270_residues MDSIFNLKGKIALITGGAGVLGSNFANVLAKQGVIVGIVSQSLEKAESTVKTLEANGGQGFAVQANVLNKEEVEKVRDFI VEKYGRLDILINAAGGNMPGATIQPDQAVYDMKSDDLQKVIDLNIIGTMLPTQVFAELFAKQKQGNIINISSASAQRPLT RVVGYSASKAAIDNFTQWMAVELAQKYGEGIRVNAISPGFFIGEQNRALLLTPEGKLTPRGEKIIDHTPMGRFGTPEDID GALLFLCSDMSKFVTGTILKVDGGFAATSI
Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]
COG id: COG1028
COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI10190704, Length=259, Percent_Identity=29.7297297297297, Blast_Score=112, Evalue=3e-25, Organism=Homo sapiens, GI40254992, Length=256, Percent_Identity=27.734375, Blast_Score=107, Evalue=9e-24, Organism=Homo sapiens, GI15277342, Length=271, Percent_Identity=31.3653136531365, Blast_Score=105, Evalue=3e-23, Organism=Homo sapiens, GI19923817, Length=264, Percent_Identity=29.1666666666667, Blast_Score=101, Evalue=8e-22, Organism=Homo sapiens, GI32483357, Length=262, Percent_Identity=29.3893129770992, Blast_Score=99, Evalue=3e-21, Organism=Homo sapiens, GI7705925, Length=263, Percent_Identity=28.5171102661597, Blast_Score=93, Evalue=3e-19, Organism=Homo sapiens, GI59889578, Length=225, Percent_Identity=30.2222222222222, Blast_Score=89, Evalue=4e-18, Organism=Homo sapiens, GI5031737, Length=264, Percent_Identity=26.5151515151515, Blast_Score=84, Evalue=2e-16, Organism=Homo sapiens, GI66933014, Length=229, Percent_Identity=30.5676855895196, Blast_Score=82, Evalue=4e-16, Organism=Homo sapiens, GI31542939, Length=267, Percent_Identity=27.7153558052434, Blast_Score=82, Evalue=7e-16, Organism=Homo sapiens, GI4503301, Length=261, Percent_Identity=27.5862068965517, Blast_Score=80, Evalue=2e-15, Organism=Homo sapiens, GI126723750, Length=262, Percent_Identity=26.7175572519084, Blast_Score=80, Evalue=3e-15, Organism=Homo sapiens, GI4758504, Length=275, Percent_Identity=28.7272727272727, Blast_Score=70, Evalue=1e-12, Organism=Homo sapiens, GI33667109, Length=198, Percent_Identity=26.7676767676768, Blast_Score=69, Evalue=4e-12, Organism=Homo sapiens, GI226371731, Length=223, Percent_Identity=25.1121076233184, Blast_Score=68, Evalue=1e-11, Organism=Homo sapiens, GI224922801, Length=178, Percent_Identity=27.5280898876405, Blast_Score=66, Evalue=3e-11, Organism=Escherichia coli, GI1787905, Length=260, Percent_Identity=33.4615384615385, Blast_Score=130, Evalue=1e-31, Organism=Escherichia coli, GI1790717, Length=265, Percent_Identity=33.5849056603774, Blast_Score=129, Evalue=2e-31, Organism=Escherichia coli, GI87082160, Length=265, Percent_Identity=33.5849056603774, Blast_Score=121, Evalue=5e-29, Organism=Escherichia coli, GI1789208, Length=267, Percent_Identity=32.9588014981273, Blast_Score=117, Evalue=9e-28, Organism=Escherichia coli, GI1788459, Length=258, Percent_Identity=32.1705426356589, Blast_Score=116, Evalue=2e-27, Organism=Escherichia coli, GI1787335, Length=261, Percent_Identity=30.6513409961686, Blast_Score=108, Evalue=4e-25, Organism=Escherichia coli, GI2367175, Length=259, Percent_Identity=29.3436293436293, Blast_Score=101, Evalue=6e-23, Organism=Escherichia coli, GI87082100, Length=269, Percent_Identity=34.9442379182156, Blast_Score=99, Evalue=3e-22, Organism=Escherichia coli, GI1786812, Length=202, Percent_Identity=30.6930693069307, Blast_Score=92, Evalue=5e-20, Organism=Escherichia coli, GI1789378, Length=261, Percent_Identity=28.735632183908, Blast_Score=85, Evalue=5e-18, Organism=Escherichia coli, GI1789057, Length=263, Percent_Identity=26.9961977186312, Blast_Score=82, Evalue=4e-17, Organism=Escherichia coli, GI2367365, Length=191, Percent_Identity=28.7958115183246, Blast_Score=64, Evalue=1e-11, Organism=Caenorhabditis elegans, GI17555706, Length=260, Percent_Identity=31.5384615384615, Blast_Score=107, Evalue=5e-24, Organism=Caenorhabditis elegans, GI25147288, Length=263, Percent_Identity=32.319391634981, Blast_Score=105, Evalue=2e-23, Organism=Caenorhabditis elegans, GI71994600, Length=258, Percent_Identity=31.7829457364341, Blast_Score=104, Evalue=6e-23, Organism=Caenorhabditis elegans, GI17536025, Length=261, Percent_Identity=31.4176245210728, Blast_Score=103, Evalue=7e-23, Organism=Caenorhabditis elegans, GI115534694, Length=267, Percent_Identity=30.3370786516854, Blast_Score=100, Evalue=8e-22, Organism=Caenorhabditis elegans, GI17560150, Length=271, Percent_Identity=31.7343173431734, Blast_Score=99, Evalue=3e-21, Organism=Caenorhabditis elegans, GI17560220, Length=271, Percent_Identity=31.3653136531365, Blast_Score=97, Evalue=1e-20, Organism=Caenorhabditis elegans, GI17560676, Length=262, Percent_Identity=25.9541984732824, Blast_Score=96, Evalue=2e-20, Organism=Caenorhabditis elegans, GI17561272, Length=262, Percent_Identity=32.824427480916, Blast_Score=96, Evalue=2e-20, Organism=Caenorhabditis elegans, GI17562990, Length=269, Percent_Identity=31.5985130111524, Blast_Score=94, Evalue=5e-20, Organism=Caenorhabditis elegans, GI17560332, Length=268, Percent_Identity=30.5970149253731, Blast_Score=94, Evalue=5e-20, Organism=Caenorhabditis elegans, GI71994604, Length=257, Percent_Identity=28.7937743190661, Blast_Score=94, Evalue=6e-20, Organism=Caenorhabditis elegans, GI17551412, Length=270, Percent_Identity=31.1111111111111, Blast_Score=93, Evalue=2e-19, Organism=Caenorhabditis elegans, GI193204405, Length=268, Percent_Identity=29.8507462686567, Blast_Score=93, Evalue=2e-19, Organism=Caenorhabditis elegans, GI17562906, Length=273, Percent_Identity=27.4725274725275, Blast_Score=92, Evalue=2e-19, Organism=Caenorhabditis elegans, GI17562910, Length=272, Percent_Identity=29.7794117647059, Blast_Score=92, Evalue=4e-19, Organism=Caenorhabditis elegans, GI17562908, Length=270, Percent_Identity=28.8888888888889, Blast_Score=91, Evalue=5e-19, Organism=Caenorhabditis elegans, GI17536651, Length=261, Percent_Identity=29.8850574712644, Blast_Score=91, Evalue=7e-19, Organism=Caenorhabditis elegans, GI17561402, Length=263, Percent_Identity=26.2357414448669, Blast_Score=90, Evalue=1e-18, Organism=Caenorhabditis elegans, GI17559104, Length=262, Percent_Identity=31.6793893129771, Blast_Score=89, Evalue=2e-18, Organism=Caenorhabditis elegans, GI17538480, Length=270, Percent_Identity=28.8888888888889, Blast_Score=89, Evalue=3e-18, Organism=Caenorhabditis elegans, GI17563726, Length=275, Percent_Identity=30.1818181818182, Blast_Score=87, Evalue=1e-17, Organism=Caenorhabditis elegans, GI17562904, Length=269, Percent_Identity=28.6245353159851, Blast_Score=85, Evalue=4e-17, Organism=Caenorhabditis elegans, GI17531453, Length=267, Percent_Identity=27.3408239700375, Blast_Score=85, Evalue=4e-17, Organism=Caenorhabditis elegans, GI17538486, Length=265, Percent_Identity=28.3018867924528, Blast_Score=85, Evalue=5e-17, Organism=Caenorhabditis elegans, GI72000259, Length=280, Percent_Identity=28.2142857142857, Blast_Score=84, Evalue=9e-17, Organism=Caenorhabditis elegans, GI17538182, Length=274, Percent_Identity=30.6569343065693, Blast_Score=82, Evalue=3e-16, Organism=Caenorhabditis elegans, GI17544670, Length=277, Percent_Identity=25.2707581227437, Blast_Score=75, Evalue=4e-14, Organism=Caenorhabditis elegans, GI17565030, Length=265, Percent_Identity=28.6792452830189, Blast_Score=74, Evalue=8e-14, Organism=Caenorhabditis elegans, GI17508895, Length=195, Percent_Identity=22.0512820512821, Blast_Score=65, Evalue=3e-11, Organism=Saccharomyces cerevisiae, GI6324126, Length=267, Percent_Identity=30.7116104868914, Blast_Score=92, Evalue=1e-19, Organism=Drosophila melanogaster, GI23397609, Length=260, Percent_Identity=30.3846153846154, Blast_Score=127, Evalue=1e-29, Organism=Drosophila melanogaster, GI21357041, Length=263, Percent_Identity=31.9391634980989, Blast_Score=121, Evalue=5e-28, Organism=Drosophila melanogaster, GI28571526, Length=267, Percent_Identity=31.0861423220974, Blast_Score=116, Evalue=2e-26, Organism=Drosophila melanogaster, GI24644337, Length=265, Percent_Identity=29.811320754717, Blast_Score=108, Evalue=4e-24, Organism=Drosophila melanogaster, GI24639444, Length=268, Percent_Identity=30.2238805970149, Blast_Score=103, Evalue=1e-22, Organism=Drosophila melanogaster, GI24644339, Length=269, Percent_Identity=29.7397769516729, Blast_Score=102, Evalue=2e-22, Organism=Drosophila melanogaster, GI21355319, Length=262, Percent_Identity=28.6259541984733, Blast_Score=101, Evalue=5e-22, Organism=Drosophila melanogaster, GI24643142, Length=264, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=9e-15, Organism=Drosophila melanogaster, GI17737361, Length=266, Percent_Identity=29.3233082706767, Blast_Score=77, Evalue=1e-14, Organism=Drosophila melanogaster, GI24649181, Length=197, Percent_Identity=26.9035532994924, Blast_Score=74, Evalue=9e-14, Organism=Drosophila melanogaster, GI24640492, Length=194, Percent_Identity=23.7113402061856, Blast_Score=72, Evalue=5e-13, Organism=Drosophila melanogaster, GI24641232, Length=201, Percent_Identity=27.8606965174129, Blast_Score=70, Evalue=2e-12, Organism=Drosophila melanogaster, GI281364892, Length=201, Percent_Identity=25.8706467661692, Blast_Score=69, Evalue=4e-12, Organism=Drosophila melanogaster, GI21358495, Length=199, Percent_Identity=28.1407035175879, Blast_Score=66, Evalue=3e-11, Organism=Drosophila melanogaster, GI21357673, Length=228, Percent_Identity=26.7543859649123, Blast_Score=66, Evalue=3e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.159 [C]
Molecular weight: Translated: 28797; Mature: 28797
Theoretical pI: Translated: 5.41; Mature: 5.41
Prosite motif: PS00061 ADH_SHORT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 2.6 %Met (Translated Protein) 3.0 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 2.6 %Met (Mature Protein) 3.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MDSIFNLKGKIALITGGAGVLGSNFANVLAKQGVIVGIVSQSLEKAESTVKTLEANGGQG CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCCCC FAVQANVLNKEEVEKVRDFIVEKYGRLDILINAAGGNMPGATIQPDQAVYDMKSDDLQKV EEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHCCCCHHHHHH IDLNIIGTMLPTQVFAELFAKQKQGNIINISSASAQRPLTRVVGYSASKAAIDNFTQWMA HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHCCHHHHHCCCCCHHHHHHHHHHHH VELAQKYGEGIRVNAISPGFFIGEQNRALLLTPEGKLTPRGEKIIDHTPMGRFGTPEDID HHHHHHHCCCEEEEEECCCEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC GALLFLCSDMSKFVTGTILKVDGGFAATSI HHHHHHHHHHHHHHCEEEEEECCCEEECCC >Mature Secondary Structure MDSIFNLKGKIALITGGAGVLGSNFANVLAKQGVIVGIVSQSLEKAESTVKTLEANGGQG CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCCCC FAVQANVLNKEEVEKVRDFIVEKYGRLDILINAAGGNMPGATIQPDQAVYDMKSDDLQKV EEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHCCCCHHHHHH IDLNIIGTMLPTQVFAELFAKQKQGNIINISSASAQRPLTRVVGYSASKAAIDNFTQWMA HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHCCHHHHHCCCCCHHHHHHHHHHHH VELAQKYGEGIRVNAISPGFFIGEQNRALLLTPEGKLTPRGEKIIDHTPMGRFGTPEDID HHHHHHHCCCEEEEEECCCEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC GALLFLCSDMSKFVTGTILKVDGGFAATSI HHHHHHHHHHHHHHCEEEEEECCCEEECCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]
Metal ions: CaCl2; KCl; MnCl2; NaCl [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]
Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]
Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]
General reaction: Redox reaction [C]
Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 9579062; 9384377 [H]