BacMap

Definition	Flavobacterium johnsoniae UW101 chromosome, complete genome.
Accession	NC_009441
Length	6,096,872

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The map label for this gene is hflB [C]

Identifier: 146301879

GI number: 146301879

Start: 4977416

End: 4978147

Strand: Direct

Name: hflB [C]

Synonym: Fjoh_4143

Alternate gene names: 146301879

Gene position: 4977416-4978147 (Clockwise)

Preceding gene: 146301878

Following gene: 146301895

Centisome position: 81.64

GC content: 34.02

Gene sequence:

>732_bases
ATGAATCTGTACGAACTTGTTGTAAACGATACTGAAAAAGTTGCACTCGATGATCTGTTTTTCAGCCAAGAAAATAAAAC
AGCCCTTCTTCAAACCATAAAGGAGCATAAATATCTTGAGGAATTAAAAAAGTACAATTTAAAAGTTGATAATAAAATAC
TGCTTCACGGGCATTCGGGCTGCGGTAAAACAACAACGGCAAAGGCAATTGCAACGGCTTTAAATAAAAACATTGTTATT
ATCAACCTCAGCACTTTAATTAATGCTAAAATTGGGGAAACTTCTAAAAACGTAAAAACGATTTTCGATAAAGCAATTCG
TGAAAAAGCAGTTTTATTTCTGGATGAATTTGACCAGATTGGAAAAAGCCGTGAAAGCGATGACAAAGATGTTGCCGAAA
TGAGACGTTTGGTAAATACTATTATTCAGCTTATAGATTATTTTCCAACAGACAGTTTATTGATTTGTGCGACGAATTTT
TACAATAGTATTGATACGGCATTACTGCGAAGATTTCAAATTAAGCTGAAATTTGAAATGCCAGACCAGCACCAATTGAA
TGTTTATTATGACAAACTGCTGGCTGATTTTCCAGAACATCTGCAGGACATTCTGCGAAAATATGATATTTCTTATGCAG
AGGCAAAAGATTATATTCACACTACAATGAAAAGGCAGATTATTGCCGAACTGGAACTTAAAGAGCAGAGAATCAACGAA
ACGATTATATAA

Upstream 100 bases:

>100_bases
AGTGGGAACTTCTATTTTTGGAAACCGCTTTTTAGGCAAAGAAATCTGGAACGAAAATATTGCAGAATAAAACCTAATTT
TGCACCAAATAAAATATCAA

Downstream 100 bases:

>100_bases
AAAAACGCCTCATATCCTGAGGCGTTTTAACAAATTAAATTCTGGTAATTAAGCTTTCGCTTTTCTTTTTACTGTACAGC
CAATTTCTTTGGTTTGTGTT

Product: ATPase central domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MNLYELVVNDTEKVALDDLFFSQENKTALLQTIKEHKYLEELKKYNLKVDNKILLHGHSGCGKTTTAKAIATALNKNIVI
INLSTLINAKIGETSKNVKTIFDKAIREKAVLFLDEFDQIGKSRESDDKDVAEMRRLVNTIIQLIDYFPTDSLLICATNF
YNSIDTALLRRFQIKLKFEMPDQHQLNVYYDKLLADFPEHLQDILRKYDISYAEAKDYIHTTMKRQIIAELELKEQRINE
TII

Sequences:

>Translated_243_residues
MNLYELVVNDTEKVALDDLFFSQENKTALLQTIKEHKYLEELKKYNLKVDNKILLHGHSGCGKTTTAKAIATALNKNIVI
INLSTLINAKIGETSKNVKTIFDKAIREKAVLFLDEFDQIGKSRESDDKDVAEMRRLVNTIIQLIDYFPTDSLLICATNF
YNSIDTALLRRFQIKLKFEMPDQHQLNVYYDKLLADFPEHLQDILRKYDISYAEAKDYIHTTMKRQIIAELELKEQRINE
TII
>Mature_243_residues
MNLYELVVNDTEKVALDDLFFSQENKTALLQTIKEHKYLEELKKYNLKVDNKILLHGHSGCGKTTTAKAIATALNKNIVI
INLSTLINAKIGETSKNVKTIFDKAIREKAVLFLDEFDQIGKSRESDDKDVAEMRRLVNTIIQLIDYFPTDSLLICATNF
YNSIDTALLRRFQIKLKFEMPDQHQLNVYYDKLLADFPEHLQDILRKYDISYAEAKDYIHTTMKRQIIAELELKEQRINE
TII

Specific function: Seems To Act As An ATP-Dependent Zinc Metallopeptidase. Involved In The Degradation Of Sigma-32. Degrades Carboxy- Terminal-Tagged Cytoplasmic Proteins. These Proteins Are Tagged With An 11-Amino-Acid Nonpolar Destabilizing Tail VIa A Mechanism Involving

COG id: COG0464

COG function: function code O; ATPases of the AAA+ class

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AAA ATPase family [H]

Homologues:

Organism=Homo sapiens, GI6005942, Length=211, Percent_Identity=32.7014218009479, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI157671927, Length=176, Percent_Identity=30.1136363636364, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4506209, Length=161, Percent_Identity=31.6770186335404, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI24497435, Length=154, Percent_Identity=32.4675324675325, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI24430151, Length=167, Percent_Identity=30.5389221556886, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI31377644, Length=140, Percent_Identity=30, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI195539395, Length=139, Percent_Identity=31.6546762589928, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI17865802, Length=137, Percent_Identity=33.5766423357664, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI45643125, Length=139, Percent_Identity=33.0935251798561, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI7019569, Length=156, Percent_Identity=30.7692307692308, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI89064750, Length=154, Percent_Identity=29.2207792207792, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI5729991, Length=154, Percent_Identity=29.2207792207792, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI24430155, Length=154, Percent_Identity=29.2207792207792, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI45643123, Length=139, Percent_Identity=33.0935251798561, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI5901990, Length=184, Percent_Identity=27.1739130434783, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI194018488, Length=129, Percent_Identity=35.6589147286822, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17505546, Length=183, Percent_Identity=30.0546448087432, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17508053, Length=166, Percent_Identity=29.5180722891566, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17532375, Length=162, Percent_Identity=34.5679012345679, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17541224, Length=153, Percent_Identity=28.1045751633987, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17541226, Length=153, Percent_Identity=28.1045751633987, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17563248, Length=161, Percent_Identity=31.055900621118, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17563250, Length=168, Percent_Identity=30.952380952381, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71987364, Length=173, Percent_Identity=29.4797687861272, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71987372, Length=173, Percent_Identity=29.4797687861272, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI193209502, Length=164, Percent_Identity=32.3170731707317, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25153574, Length=164, Percent_Identity=32.3170731707317, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17531535, Length=146, Percent_Identity=34.2465753424658, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI25144281, Length=154, Percent_Identity=33.1168831168831, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144278, Length=154, Percent_Identity=33.1168831168831, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17554786, Length=138, Percent_Identity=35.5072463768116, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25146157, Length=208, Percent_Identity=28.3653846153846, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508701, Length=136, Percent_Identity=33.0882352941176, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI86561515, Length=187, Percent_Identity=28.8770053475936, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17554264, Length=153, Percent_Identity=30.0653594771242, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17537217, Length=161, Percent_Identity=33.5403726708075, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324000, Length=183, Percent_Identity=34.9726775956284, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323429, Length=173, Percent_Identity=31.7919075144509, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325431, Length=200, Percent_Identity=27.5, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324833, Length=151, Percent_Identity=31.7880794701987, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322704, Length=169, Percent_Identity=31.3609467455621, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6321465, Length=142, Percent_Identity=32.3943661971831, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322651, Length=149, Percent_Identity=33.5570469798658, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320077, Length=191, Percent_Identity=33.5078534031414, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324691, Length=184, Percent_Identity=29.3478260869565, Blast_Score=73, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6325183, Length=216, Percent_Identity=28.7037037037037, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321390, Length=150, Percent_Identity=32, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320602, Length=134, Percent_Identity=32.089552238806, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325281, Length=177, Percent_Identity=29.9435028248588, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322994, Length=167, Percent_Identity=30.5389221556886, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323736, Length=178, Percent_Identity=27.5280898876405, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320197, Length=164, Percent_Identity=31.0975609756098, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320887, Length=168, Percent_Identity=30.3571428571429, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24652279, Length=129, Percent_Identity=37.2093023255814, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17137738, Length=184, Percent_Identity=31.5217391304348, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI161076488, Length=161, Percent_Identity=34.7826086956522, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24640100, Length=134, Percent_Identity=33.5820895522388, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI17137560, Length=161, Percent_Identity=34.7826086956522, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI161076486, Length=161, Percent_Identity=34.7826086956522, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24663015, Length=168, Percent_Identity=29.7619047619048, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24639486, Length=177, Percent_Identity=28.8135593220339, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19920408, Length=138, Percent_Identity=36.231884057971, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI18860063, Length=154, Percent_Identity=29.8701298701299, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45552965, Length=141, Percent_Identity=34.0425531914894, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI281365776, Length=141, Percent_Identity=34.0425531914894, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24660075, Length=141, Percent_Identity=34.0425531914894, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI20129423, Length=138, Percent_Identity=29.7101449275362, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24651451, Length=137, Percent_Identity=35.036496350365, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24649446, Length=167, Percent_Identity=29.940119760479, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI18859657, Length=143, Percent_Identity=30.7692307692308, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24581396, Length=197, Percent_Identity=27.9187817258883, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI281361487, Length=149, Percent_Identity=29.5302013422819, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363136, Length=125, Percent_Identity=35.2, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI78707192, Length=125, Percent_Identity=35.2, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17738151, Length=151, Percent_Identity=32.4503311258278, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 28221; Mature: 28221

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLYELVVNDTEKVALDDLFFSQENKTALLQTIKEHKYLEELKKYNLKVDNKILLHGHSG
CCHHHEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
CGKTTTAKAIATALNKNIVIINLSTLINAKIGETSKNVKTIFDKAIREKAVLFLDEFDQI
CCCHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GKSRESDDKDVAEMRRLVNTIIQLIDYFPTDSLLICATNFYNSIDTALLRRFQIKLKFEM
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHEEEEECC
PDQHQLNVYYDKLLADFPEHLQDILRKYDISYAEAKDYIHTTMKRQIIAELELKEQRINE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TII
CCC
>Mature Secondary Structure
MNLYELVVNDTEKVALDDLFFSQENKTALLQTIKEHKYLEELKKYNLKVDNKILLHGHSG
CCHHHEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
CGKTTTAKAIATALNKNIVIINLSTLINAKIGETSKNVKTIFDKAIREKAVLFLDEFDQI
CCCHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GKSRESDDKDVAEMRRLVNTIIQLIDYFPTDSLLICATNFYNSIDTALLRRFQIKLKFEM
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHEEEEECC
PDQHQLNVYYDKLLADFPEHLQDILRKYDISYAEAKDYIHTTMKRQIIAELELKEQRINE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TII
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Metalloendopeptidases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]