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The map label for this gene is oprM [H]

Identifier: 146301866

GI number: 146301866

Start: 4963148

End: 4964554

Strand: Reverse

Name: oprM [H]

Synonym: Fjoh_4130

Alternate gene names: 146301866

Gene position: 4964554-4963148 (Counterclockwise)

Preceding gene: 146301867

Following gene: 146301865

Centisome position: 81.43

GC content: 36.96

Gene sequence:

>1407_bases
ATGACGAATAGTTCAAATAAATATATTCTTATGGTAATTGCAGCCGCGCTTTTAAGTGCGTGCTCAATTACCAAAAAGTA
CGAACGTCCAGCTGCACTCTCGACAGACAAGCTGTATCGTGATGAGGCTTCTGCCGATACGACTACAATTGCTGATATGC
CGTGGCAGAGTGTTTTTAATGACGAAAAGCTGAATGCTTTAATCCAGAAAGGATTAGATCAAAACCTGAACCTGAAAAAT
GCTATTGAAAATATAGTACAGGCAAGAGCTTCATTGCGCCAGAGTAAACTGGCTTATTACCCAACATTACAGCTGGATGC
AAGTGCAACGCACACGAAACAGTCAGAAGCGGGGCTTAACTTTCCTGCCGGAATTAATATCAATACCCTGACTACAACTT
ATAAATTAGGTGTAAGTACGTCTTGGGAAGCTGATATCTGGGGAAAACTAAGCAGTTCTAAAAGAGCGGCCTTAGCTAGT
TATTTAGCCACAGATGCTGCCAAACAAGCCATTCAGACACAATTAATTTCTGATATTGCCAATAATTATTTTATGCTTTT
GGCTTACGATAAATCGTTAAAAATCACTCAGGAAACATTAGAAAGCCGTATTAAAAATGTTGAAACAATTAAAGCTTTAA
AAGAAGGAGCAATTGTAACAGGAGCGGCAGTTGTACAGAGTGAAGCCAATCAGCATGCAGCAGAAGTTTTAATTCCGGAT
TTAAAACAAAGTATACGCGAAACCGAAAACGCTTTGAATATCTTATTAGGGCAAGCTCCGGGACCAATTGACAGAGGAGA
ATTAGAAACTCAGGTTATTCCGGAAAACATTGCAATTGGAGTTTCTTCTCAGTTATTAGAAAACCGTCCTGATGTTCGTC
AGGCTGAATTTAATTTCAGAGTAGCTTTTGAATCTACAAATCTGGCTAAAACCTATTTTTATCCAAGTTTAACGCTTACG
GCAAGCGGCGGTTTTTCGAATTTAGAATTAAAAGATTTCTTTACTAATTCTATTTTTTATTCTATTATTGGAGGATTAAC
ACAGCCAATTTTTAATCAGGGTAAAAACAAGCTCAGATTAACAACAGCACAGTCACAACAATTACAGGCTTATAATAATT
TCCAGCAAAGTCTTTTAACAGCGGGTCAGGAAGTTTCTAATGCTTTGTATTCATACCAAATGGCAGTCGAAAAAGAAGAT
TCAAGAAAAAAACAAATTGAAGCTTTAGAAAAAGCAGTCGATTTTACACAGCAGCTTCTTGAATACAGTTCAGCAACAAA
TTATACAGACGTATTAACTTCTGAACAAAATTTACTGGCAGCACAGTTAAGCGGTATTAATGATAATTTACAAAAATTAC
AAGCCGTTGTAAACTTATACAGAGCTTTAGGCGGAGGATGGAAATAA

Upstream 100 bases:

>100_bases
TAAGCGGTCCGGCAAAAGATGGTTATGATGATGACGACGATGATGAGGATGAAATTCAGTTAATAGAAGCTCACAAAGAG
TAAAATTTAATTAAAAAGAG

Downstream 100 bases:

>100_bases
ATATTTTCCTATAAATAATTAACGTTGGTCGTTAAAAATATAACCTTGGTCTAAATAATAATTTAATACTAAAAATTGTA
TTTAACTTTGAATTGTTAAG

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTNSSNKYILMVIAAALLSACSITKKYERPAALSTDKLYRDEASADTTTIADMPWQSVFNDEKLNALIQKGLDQNLNLKN
AIENIVQARASLRQSKLAYYPTLQLDASATHTKQSEAGLNFPAGININTLTTTYKLGVSTSWEADIWGKLSSSKRAALAS
YLATDAAKQAIQTQLISDIANNYFMLLAYDKSLKITQETLESRIKNVETIKALKEGAIVTGAAVVQSEANQHAAEVLIPD
LKQSIRETENALNILLGQAPGPIDRGELETQVIPENIAIGVSSQLLENRPDVRQAEFNFRVAFESTNLAKTYFYPSLTLT
ASGGFSNLELKDFFTNSIFYSIIGGLTQPIFNQGKNKLRLTTAQSQQLQAYNNFQQSLLTAGQEVSNALYSYQMAVEKED
SRKKQIEALEKAVDFTQQLLEYSSATNYTDVLTSEQNLLAAQLSGINDNLQKLQAVVNLYRALGGGWK

Sequences:

>Translated_468_residues
MTNSSNKYILMVIAAALLSACSITKKYERPAALSTDKLYRDEASADTTTIADMPWQSVFNDEKLNALIQKGLDQNLNLKN
AIENIVQARASLRQSKLAYYPTLQLDASATHTKQSEAGLNFPAGININTLTTTYKLGVSTSWEADIWGKLSSSKRAALAS
YLATDAAKQAIQTQLISDIANNYFMLLAYDKSLKITQETLESRIKNVETIKALKEGAIVTGAAVVQSEANQHAAEVLIPD
LKQSIRETENALNILLGQAPGPIDRGELETQVIPENIAIGVSSQLLENRPDVRQAEFNFRVAFESTNLAKTYFYPSLTLT
ASGGFSNLELKDFFTNSIFYSIIGGLTQPIFNQGKNKLRLTTAQSQQLQAYNNFQQSLLTAGQEVSNALYSYQMAVEKED
SRKKQIEALEKAVDFTQQLLEYSSATNYTDVLTSEQNLLAAQLSGINDNLQKLQAVVNLYRALGGGWK
>Mature_467_residues
TNSSNKYILMVIAAALLSACSITKKYERPAALSTDKLYRDEASADTTTIADMPWQSVFNDEKLNALIQKGLDQNLNLKNA
IENIVQARASLRQSKLAYYPTLQLDASATHTKQSEAGLNFPAGININTLTTTYKLGVSTSWEADIWGKLSSSKRAALASY
LATDAAKQAIQTQLISDIANNYFMLLAYDKSLKITQETLESRIKNVETIKALKEGAIVTGAAVVQSEANQHAAEVLIPDL
KQSIRETENALNILLGQAPGPIDRGELETQVIPENIAIGVSSQLLENRPDVRQAEFNFRVAFESTNLAKTYFYPSLTLTA
SGGFSNLELKDFFTNSIFYSIIGGLTQPIFNQGKNKLRLTTAQSQQLQAYNNFQQSLLTAGQEVSNALYSYQMAVEKEDS
RKKQIEALEKAVDFTQQLLEYSSATNYTDVLTSEQNLLAAQLSGINDNLQKLQAVVNLYRALGGGWK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=477, Percent_Identity=28.0922431865828, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI2367348, Length=467, Percent_Identity=23.5546038543897, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51518; Mature: 51386

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSSNKYILMVIAAALLSACSITKKYERPAALSTDKLYRDEASADTTTIADMPWQSVFN
CCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCEECCCCHHHHCC
DEKLNALIQKGLDQNLNLKNAIENIVQARASLRQSKLAYYPTLQLDASATHTKQSEAGLN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCEEEECCCCCCCCHHCCCCC
FPAGININTLTTTYKLGVSTSWEADIWGKLSSSKRAALASYLATDAAKQAIQTQLISDIA
CCCCCCEEEEEEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NNYFMLLAYDKSLKITQETLESRIKNVETIKALKEGAIVTGAAVVQSEANQHAAEVLIPD
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHH
LKQSIRETENALNILLGQAPGPIDRGELETQVIPENIAIGVSSQLLENRPDVRQAEFNFR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCHHEECCHHHHHHCCCCCEECCCEEE
VAFESTNLAKTYFYPSLTLTASGGFSNLELKDFFTNSIFYSIIGGLTQPIFNQGKNKLRL
EEEECCCCEEEEEECCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
TTAQSQQLQAYNNFQQSLLTAGQEVSNALYSYQMAVEKEDSRKKQIEALEKAVDFTQQLL
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
EYSSATNYTDVLTSEQNLLAAQLSGINDNLQKLQAVVNLYRALGGGWK
HHHCCCCHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TNSSNKYILMVIAAALLSACSITKKYERPAALSTDKLYRDEASADTTTIADMPWQSVFN
CCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCEECCCCHHHHCC
DEKLNALIQKGLDQNLNLKNAIENIVQARASLRQSKLAYYPTLQLDASATHTKQSEAGLN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCEEEECCCCCCCCHHCCCCC
FPAGININTLTTTYKLGVSTSWEADIWGKLSSSKRAALASYLATDAAKQAIQTQLISDIA
CCCCCCEEEEEEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NNYFMLLAYDKSLKITQETLESRIKNVETIKALKEGAIVTGAAVVQSEANQHAAEVLIPD
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHH
LKQSIRETENALNILLGQAPGPIDRGELETQVIPENIAIGVSSQLLENRPDVRQAEFNFR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCHHEECCHHHHHHCCCCCEECCCEEE
VAFESTNLAKTYFYPSLTLTASGGFSNLELKDFFTNSIFYSIIGGLTQPIFNQGKNKLRL
EEEECCCCEEEEEECCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
TTAQSQQLQAYNNFQQSLLTAGQEVSNALYSYQMAVEKEDSRKKQIEALEKAVDFTQQLL
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
EYSSATNYTDVLTSEQNLLAAQLSGINDNLQKLQAVVNLYRALGGGWK
HHHCCCCHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]