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The map label for this gene is pgl [H]

Identifier: 146301824

GI number: 146301824

Start: 4894247

End: 4895815

Strand: Reverse

Name: pgl [H]

Synonym: Fjoh_4088

Alternate gene names: 146301824

Gene position: 4895815-4894247 (Counterclockwise)

Preceding gene: 146301825

Following gene: 146301823

Centisome position: 80.3

GC content: 36.07

Gene sequence:

>1569_bases
ATGAAAAACATACTAATAATACTCTGCTTCATAACTGGATTGAACACCACTTTCGCAAACGACGGCTGGTTAAACATCTT
AAAAGAAGGAGGAAATAACAAAGGAATAAAATGCACTCAAGCCATTCAAAATGCCATCGAAAAAGCATCTAAAAACGGCG
GAGGAACCATCTTTTTTCCAGCAGGAGAATATTTAACAGGAGCTTTAACACTAAGAAGCAACATTACGATTCATTTAGAT
TCTGGAGCACTTTTAAAGTTTTCAGAGAACTTCGATGATTTCCTTCCTTACGTAGAAATGCGTTACGAAGGAATTGTAAT
GAAGAGTTTCCAACCTTTATTTTATGCTAAAGATGTACAAAATATCACTATTACAGGAAGAGGCGTAATCGACGGACAAG
GAAAAGCGTGGTGGAATGAAGTGTACCGAATCGAAACGGCTAAGGAACCACTTCCTCCAACTAAATATCAAACTATGTGG
GAAGAGCAAAATAAAGGCCTTTACACAGAACCATATTACAAAAGAACGGTTGATAAAAAGTTTTTCAGACCTTCTTTCTT
TCAGGCTTATAATTGCAAAAATATTTTAATAGAAGGTGTAACCTTTCAAAATTCACCTTTTTGGACAATCAACCCGGAAT
TCTGTGATAATGTAACTGTTACGGGAATTTCGATTTTCAATCCACATTCACCAAATACAGATGGAATCAATCCTTCTTCT
TGTACCAATGTTCACATTTCAAACTGCCATATCAGCGTTGGCGATGATTGTATCACGATTAAATCAGGAAGAGACGGTGA
CGGACGTAAATATGGGAAAGCGACAGAAAATGTAACCATTACAAACTGTACGATGTTAAGTGGTCACGGCGGTGTTGTTA
TTGGTAGTGAAATGTCGGGTGGAATCAAAAAAATCACAATATCGAACTGTGTTTTTGATGGAACAGATCGCGGAATCCGT
ATCAAATCGGCTCGCGGAAGAGGAGGAGTTGTAGAAGATATTCGCGTCGATAATATCGTCATGAAAAACATCAAAGAAGA
AGCTATCGTTTTGAGCCTTTTCTACGATAAAGGAACTCAGGTTGAACCTGTAACCGAGAAAACGCCGATTTTCAGAAACA
TTCATATGAGCAACATTACAGCTTCAAACGTAAATAAAGCAGGACAGATTTTGGGAATCACCGAAATGCCAATTCAAAAC
ATCACTTTTTCTAACATCAATATGGATGGAAAAGAAGGTTTTACAGTAAGTACGGCGACAGATGTTGAATTCCATGATGT
AAAAGTGAATGCAACTGTAGGTTCGTCTTTCAAAATATCAGATTCAAAAAATATTATTTTAGACAATGTTGGATCTTCAA
CACCAATAAAAGGAGTTCCCGTTATCAAATTAGATAATGTTTCAAATGCGTTGATCAACAATAATTTTCCATTCAATCCA
ACCGATATTTTTATCGAGGCGGATGGGAAAGACACTAAAAACATTTTCTTGAAAAACAATGTTTTAAACAACGTAACAAC
GAAAATCAAAAAAGGAGCTTCTTTAGATAAAAAAGCAATTGCAGAATAA

Upstream 100 bases:

>100_bases
GAGTTTCTTGTGAAGATTTCTCCTTCGTCGAAATGACAAAAAATGAGAGTAAAAATCTGTGCTAATCATTTTAATCTGTG
GCAAAAGAAAAAAACAAACT

Downstream 100 bases:

>100_bases
TGCGTTCCTGCAAGGTTTTCAAAACCTTGTAGGTTTAGTTTGTGCACATAGAAATACCTACGAGGTTTTGGAAACCTCGC
AGGAAAAGAAAACAAGAAAT

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: PGL; Pectinase [H]

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MKNILIILCFITGLNTTFANDGWLNILKEGGNNKGIKCTQAIQNAIEKASKNGGGTIFFPAGEYLTGALTLRSNITIHLD
SGALLKFSENFDDFLPYVEMRYEGIVMKSFQPLFYAKDVQNITITGRGVIDGQGKAWWNEVYRIETAKEPLPPTKYQTMW
EEQNKGLYTEPYYKRTVDKKFFRPSFFQAYNCKNILIEGVTFQNSPFWTINPEFCDNVTVTGISIFNPHSPNTDGINPSS
CTNVHISNCHISVGDDCITIKSGRDGDGRKYGKATENVTITNCTMLSGHGGVVIGSEMSGGIKKITISNCVFDGTDRGIR
IKSARGRGGVVEDIRVDNIVMKNIKEEAIVLSLFYDKGTQVEPVTEKTPIFRNIHMSNITASNVNKAGQILGITEMPIQN
ITFSNINMDGKEGFTVSTATDVEFHDVKVNATVGSSFKISDSKNIILDNVGSSTPIKGVPVIKLDNVSNALINNNFPFNP
TDIFIEADGKDTKNIFLKNNVLNNVTTKIKKGASLDKKAIAE

Sequences:

>Translated_522_residues
MKNILIILCFITGLNTTFANDGWLNILKEGGNNKGIKCTQAIQNAIEKASKNGGGTIFFPAGEYLTGALTLRSNITIHLD
SGALLKFSENFDDFLPYVEMRYEGIVMKSFQPLFYAKDVQNITITGRGVIDGQGKAWWNEVYRIETAKEPLPPTKYQTMW
EEQNKGLYTEPYYKRTVDKKFFRPSFFQAYNCKNILIEGVTFQNSPFWTINPEFCDNVTVTGISIFNPHSPNTDGINPSS
CTNVHISNCHISVGDDCITIKSGRDGDGRKYGKATENVTITNCTMLSGHGGVVIGSEMSGGIKKITISNCVFDGTDRGIR
IKSARGRGGVVEDIRVDNIVMKNIKEEAIVLSLFYDKGTQVEPVTEKTPIFRNIHMSNITASNVNKAGQILGITEMPIQN
ITFSNINMDGKEGFTVSTATDVEFHDVKVNATVGSSFKISDSKNIILDNVGSSTPIKGVPVIKLDNVSNALINNNFPFNP
TDIFIEADGKDTKNIFLKNNVLNNVTTKIKKGASLDKKAIAE
>Mature_522_residues
MKNILIILCFITGLNTTFANDGWLNILKEGGNNKGIKCTQAIQNAIEKASKNGGGTIFFPAGEYLTGALTLRSNITIHLD
SGALLKFSENFDDFLPYVEMRYEGIVMKSFQPLFYAKDVQNITITGRGVIDGQGKAWWNEVYRIETAKEPLPPTKYQTMW
EEQNKGLYTEPYYKRTVDKKFFRPSFFQAYNCKNILIEGVTFQNSPFWTINPEFCDNVTVTGISIFNPHSPNTDGINPSS
CTNVHISNCHISVGDDCITIKSGRDGDGRKYGKATENVTITNCTMLSGHGGVVIGSEMSGGIKKITISNCVFDGTDRGIR
IKSARGRGGVVEDIRVDNIVMKNIKEEAIVLSLFYDKGTQVEPVTEKTPIFRNIHMSNITASNVNKAGQILGITEMPIQN
ITFSNINMDGKEGFTVSTATDVEFHDVKVNATVGSSFKISDSKNIILDNVGSSTPIKGVPVIKLDNVSNALINNNFPFNP
TDIFIEADGKDTKNIFLKNNVLNNVTTKIKKGASLDKKAIAE

Specific function: Seems to regulates the surface properties of the bacterium in the presence of plant cells or plant cell extracts [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 57492; Mature: 57492

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS00502 POLYGALACTURONASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNILIILCFITGLNTTFANDGWLNILKEGGNNKGIKCTQAIQNAIEKASKNGGGTIFFP
CCCEEEEEEEECCCCCCCCCCCHHHHEECCCCCCCEEHHHHHHHHHHHHCCCCCEEEEEE
AGEYLTGALTLRSNITIHLDSGALLKFSENFDDFLPYVEMRYEGIVMKSFQPLFYAKDVQ
CCCCEEEEEEEECEEEEEECCCEEEEECCCHHHHCCHHEEEECEEEEECCCCEEEECCCC
NITITGRGVIDGQGKAWWNEVYRIETAKEPLPPTKYQTMWEEQNKGLYTEPYYKRTVDKK
EEEEEECEEECCCCCHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHH
FFRPSFFQAYNCKNILIEGVTFQNSPFWTINPEFCDNVTVTGISIFNPHSPNTDGINPSS
HCCCCHHHHCCCCEEEEEEEEECCCCEEEECHHHCCCEEEEEEEEECCCCCCCCCCCCCC
CTNVHISNCHISVGDDCITIKSGRDGDGRKYGKATENVTITNCTMLSGHGGVVIGSEMSG
CCEEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCEEEEEEEEEECCCCEEECCCCCC
GIKKITISNCVFDGTDRGIRIKSARGRGGVVEDIRVDNIVMKNIKEEAIVLSLFYDKGTQ
CEEEEEEEEEEECCCCCCEEEEECCCCCCCEEEEEECHHHHHCCCCCEEEEEEEECCCCE
VEPVTEKTPIFRNIHMSNITASNVNKAGQILGITEMPIQNITFSNINMDGKEGFTVSTAT
ECCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCEEEEEEC
DVEFHDVKVNATVGSSFKISDSKNIILDNVGSSTPIKGVPVIKLDNVSNALINNNFPFNP
CCEEEEEEEEEEECCEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCEECCCCCCCC
TDIFIEADGKDTKNIFLKNNVLNNVTTKIKKGASLDKKAIAE
EEEEEECCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHCC
>Mature Secondary Structure
MKNILIILCFITGLNTTFANDGWLNILKEGGNNKGIKCTQAIQNAIEKASKNGGGTIFFP
CCCEEEEEEEECCCCCCCCCCCHHHHEECCCCCCCEEHHHHHHHHHHHHCCCCCEEEEEE
AGEYLTGALTLRSNITIHLDSGALLKFSENFDDFLPYVEMRYEGIVMKSFQPLFYAKDVQ
CCCCEEEEEEEECEEEEEECCCEEEEECCCHHHHCCHHEEEECEEEEECCCCEEEECCCC
NITITGRGVIDGQGKAWWNEVYRIETAKEPLPPTKYQTMWEEQNKGLYTEPYYKRTVDKK
EEEEEECEEECCCCCHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHH
FFRPSFFQAYNCKNILIEGVTFQNSPFWTINPEFCDNVTVTGISIFNPHSPNTDGINPSS
HCCCCHHHHCCCCEEEEEEEEECCCCEEEECHHHCCCEEEEEEEEECCCCCCCCCCCCCC
CTNVHISNCHISVGDDCITIKSGRDGDGRKYGKATENVTITNCTMLSGHGGVVIGSEMSG
CCEEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCEEEEEEEEEECCCCEEECCCCCC
GIKKITISNCVFDGTDRGIRIKSARGRGGVVEDIRVDNIVMKNIKEEAIVLSLFYDKGTQ
CEEEEEEEEEEECCCCCCEEEEECCCCCCCEEEEEECHHHHHCCCCCEEEEEEEECCCCE
VEPVTEKTPIFRNIHMSNITASNVNKAGQILGITEMPIQNITFSNINMDGKEGFTVSTAT
ECCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCEEEEEEC
DVEFHDVKVNATVGSSFKISDSKNIILDNVGSSTPIKGVPVIKLDNVSNALINNNFPFNP
CCEEEEEEEEEEECCEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCEECCCCCCCC
TDIFIEADGKDTKNIFLKNNVLNNVTTKIKKGASLDKKAIAE
EEEEEECCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1860822 [H]