| Definition | Flavobacterium johnsoniae UW101 chromosome, complete genome. |
|---|---|
| Accession | NC_009441 |
| Length | 6,096,872 |
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The map label for this gene is lacZ [H]
Identifier: 146301810
GI number: 146301810
Start: 4865726
End: 4868146
Strand: Reverse
Name: lacZ [H]
Synonym: Fjoh_4074
Alternate gene names: 146301810
Gene position: 4868146-4865726 (Counterclockwise)
Preceding gene: 146301811
Following gene: 146301809
Centisome position: 79.85
GC content: 34.94
Gene sequence:
>2421_bases ATGATTAAACATAAAAACATATTTCAAAAAATTACAATTGCCTTATTGTTTTCAGTATCCATTTTTGCACAAAAACAAGC CAGAATTGTCGAAGATTTCAACAAAAATTGGAACTTCAAATTAGGAGATTATCCAGAAGCAATAAATGCCAATTTCACTG CATCAGATTGGAGAACGCTTCAATTACCACACGATTGGAGTATTGAAGGCGCTTTTGATAAGGATGCTAAAACCAAGCAA GCACAAGGATTTTTACCTGCTGGAAAAGGCTGGTATCGCAAAGTATTTACAGTTCCGGCGAACTGGAAAAACAAAACTAT TTCAATTGAATTTGACGGTGTTTTTAAGAACAGTGAAGTTTTCATAAATGGAAAATCATTAGGAGTTCGCCCAAATGGTT ATATTTCTTTTGGATATGACTTGACGCAATATTTAAACTTTGGAAAATCAAATACAATTGCCGTAAAAGTCGATAACGAT GCACAACCGAATTCTAGATGGTACACAGGTTCTGGGATTTACAGAAATGTACGTTTAGTTGCCAGTGAAAAACTGCACGT TGGAAAATGGGGAACTTTTGTAACAACGCCAGAAGTTTCAGCAGAAAAATCAAAGATACATCTAGAAGTTACGATTGATA ATGATAATGCTTCTGCGAAAGAATTTAAACTGGTTACTTCGATTATAAATGCAGAAAATAAAGAAGTTGTCAATTTTACA TCAACAGAAAAAATTGGTGCTAAAACCTCAGAAAAAAAGATTCATGATTTGGTTTTAAATCAGCCGAAATTATGGAGTAC AGAAAATCCGTATTTGTATAAAGTCATTACAAAGGTTTACGAGAAATCGAAATTGGTTGATAATTATGAAACTCCACTTG GGTTTAGATATTTTAATTTTGATTCAGAAAAAGGATTTTCTTTAAATGGTGTTCCAACCAAAATTTACGGCGTTTGTTTG CATCACGATAATGGTGCTTTGGGTGCGGTAGAAAATATTCATGCCGTAAGAAGAAAGCTGGCTTTAATGAAAGAAATGGG AGCAAACGCTATCAGAATGTCTCATAATCCACATTCGTTGGAAATGATGCAATTGTGCGACGAAATGGGGTTTATCGTTC AAGATGAAGCTTTTGATGTTTGGAAAAAGAAAAAAGTAACCAACGATTATCATAAAGATTGGGATGCTTGGCACAAACAA GATTTAGAAGATTTTATTAAAAGAGATCGTAATCATCCATCGGTTATGATGTGGAGTATTGGTAACGAAATTAGAGAACA GTTTGATTCGACAGGAATTGCGATTACCAGAGAATTGGCTAAAATTGTAAAATCTTTAGATAAAACTCGTCCTGTAACTT CGGCTTTGACAGAAAATGTAATTGAGAAAAACTTCATTTATCAGTCAGGAGCTTTGGATCTCTTAGGATTCAATTACAAA CATGAAGATTATAAAGATTTTCCAACGAAATTTAAAGGACAAAAAATATTGGCTTCAGAAAGTGTTTCGGCTTTAGAAAC ACGTGGGCATTATGATTTTCCTGACGGAATTAAAGCCTGGCCAACAAAACACGGAGCTCCGTTTGACGGAAATGCCGATT GGACGGTTTCGGCTTATGATCAGGTAAAATCGTATTGGGGCGCAACGCACGAAGAAAACTGGAAAACGATTAAAAGTCAG GATTTCATGGCGGGAACTTTTATCTGGACAGGTTTTGATTATATCGGAGAACCAGATCCGTATCCGTTTCCTGCAAGAAG TTCGTATTTCGGAATCGTTGATTTAGCCGGACTTCCAAAAGATGTGTATTATATGTATCAAAGTGAATGGTCGAACAAAA CGGTTCTGCATATTTTACCGCATTGGAACTGGAAAAAAGATCAAGAAGTCGAAGTTTGGGCGTATTACAATAATGCTGAT GAAGTAGAATTATTCTTAAACGGAAAGTCGTTAGGAAAGAAAGCAAAACAAAATGATGATTTGCATATTTCTTGGAGAGT AAAATTTGAGCCAGGAACATTAAAAGCGGTTTCGAGAAAGTACGGAAAGGTGGTTTTAGAAAAAGAAATAAAGACGGCTG GAGAAGCTTCTAAAATCGATTTAAAAGCAGATAAAACTTCAATCAAAAACGATACTTACGATTTGGTTTATGTAACTGTT TCTATGACTGATAAAGATGGAAATTTAGTCCCAAACGCAATGGATTTAGTCAATTTTGAAGTTTCTGGCGGAGGAAAATT AGTTGGAGTTGATAATGGTTATCAGGCTAATTTGGATTCTTTTAAAGCCAATTCCTGTAAATTGTTTAATGGAAAATGTG TTGCGATTATTCAGTCGAATGGGAAGAAAGAAAATATTCAGCTGAAAGCGATGGCAGGAAATGGAATTCCGTTTTCAGCA ATTCAGATAAACGTTAATTAA
Upstream 100 bases:
>100_bases TTGCTCGACAAAGATTGATGACTTAGTTTGCGTGGGCTTCGACTCCGCTCAGCCTGACAAAACCGAAGTAGAATTTAAGA GTAGAATAAAAAACATATTT
Downstream 100 bases:
>100_bases TTTTATATGTCGCCCCGCTGGGGCTTTGATTCTCTAATCTATTATTTTCTTCTATAAATATTTAAGCTCCGCTGGAGCTT TTAAATTACAATTTAGAAAA
Product: glycoside hydrolase family protein
Products: NA
Alternate protein names: Beta-gal; Lactase [H]
Number of amino acids: Translated: 806; Mature: 806
Protein sequence:
>806_residues MIKHKNIFQKITIALLFSVSIFAQKQARIVEDFNKNWNFKLGDYPEAINANFTASDWRTLQLPHDWSIEGAFDKDAKTKQ AQGFLPAGKGWYRKVFTVPANWKNKTISIEFDGVFKNSEVFINGKSLGVRPNGYISFGYDLTQYLNFGKSNTIAVKVDND AQPNSRWYTGSGIYRNVRLVASEKLHVGKWGTFVTTPEVSAEKSKIHLEVTIDNDNASAKEFKLVTSIINAENKEVVNFT STEKIGAKTSEKKIHDLVLNQPKLWSTENPYLYKVITKVYEKSKLVDNYETPLGFRYFNFDSEKGFSLNGVPTKIYGVCL HHDNGALGAVENIHAVRRKLALMKEMGANAIRMSHNPHSLEMMQLCDEMGFIVQDEAFDVWKKKKVTNDYHKDWDAWHKQ DLEDFIKRDRNHPSVMMWSIGNEIREQFDSTGIAITRELAKIVKSLDKTRPVTSALTENVIEKNFIYQSGALDLLGFNYK HEDYKDFPTKFKGQKILASESVSALETRGHYDFPDGIKAWPTKHGAPFDGNADWTVSAYDQVKSYWGATHEENWKTIKSQ DFMAGTFIWTGFDYIGEPDPYPFPARSSYFGIVDLAGLPKDVYYMYQSEWSNKTVLHILPHWNWKKDQEVEVWAYYNNAD EVELFLNGKSLGKKAKQNDDLHISWRVKFEPGTLKAVSRKYGKVVLEKEIKTAGEASKIDLKADKTSIKNDTYDLVYVTV SMTDKDGNLVPNAMDLVNFEVSGGGKLVGVDNGYQANLDSFKANSCKLFNGKCVAIIQSNGKKENIQLKAMAGNGIPFSA IQINVN
Sequences:
>Translated_806_residues MIKHKNIFQKITIALLFSVSIFAQKQARIVEDFNKNWNFKLGDYPEAINANFTASDWRTLQLPHDWSIEGAFDKDAKTKQ AQGFLPAGKGWYRKVFTVPANWKNKTISIEFDGVFKNSEVFINGKSLGVRPNGYISFGYDLTQYLNFGKSNTIAVKVDND AQPNSRWYTGSGIYRNVRLVASEKLHVGKWGTFVTTPEVSAEKSKIHLEVTIDNDNASAKEFKLVTSIINAENKEVVNFT STEKIGAKTSEKKIHDLVLNQPKLWSTENPYLYKVITKVYEKSKLVDNYETPLGFRYFNFDSEKGFSLNGVPTKIYGVCL HHDNGALGAVENIHAVRRKLALMKEMGANAIRMSHNPHSLEMMQLCDEMGFIVQDEAFDVWKKKKVTNDYHKDWDAWHKQ DLEDFIKRDRNHPSVMMWSIGNEIREQFDSTGIAITRELAKIVKSLDKTRPVTSALTENVIEKNFIYQSGALDLLGFNYK HEDYKDFPTKFKGQKILASESVSALETRGHYDFPDGIKAWPTKHGAPFDGNADWTVSAYDQVKSYWGATHEENWKTIKSQ DFMAGTFIWTGFDYIGEPDPYPFPARSSYFGIVDLAGLPKDVYYMYQSEWSNKTVLHILPHWNWKKDQEVEVWAYYNNAD EVELFLNGKSLGKKAKQNDDLHISWRVKFEPGTLKAVSRKYGKVVLEKEIKTAGEASKIDLKADKTSIKNDTYDLVYVTV SMTDKDGNLVPNAMDLVNFEVSGGGKLVGVDNGYQANLDSFKANSCKLFNGKCVAIIQSNGKKENIQLKAMAGNGIPFSA IQINVN >Mature_806_residues MIKHKNIFQKITIALLFSVSIFAQKQARIVEDFNKNWNFKLGDYPEAINANFTASDWRTLQLPHDWSIEGAFDKDAKTKQ AQGFLPAGKGWYRKVFTVPANWKNKTISIEFDGVFKNSEVFINGKSLGVRPNGYISFGYDLTQYLNFGKSNTIAVKVDND AQPNSRWYTGSGIYRNVRLVASEKLHVGKWGTFVTTPEVSAEKSKIHLEVTIDNDNASAKEFKLVTSIINAENKEVVNFT STEKIGAKTSEKKIHDLVLNQPKLWSTENPYLYKVITKVYEKSKLVDNYETPLGFRYFNFDSEKGFSLNGVPTKIYGVCL HHDNGALGAVENIHAVRRKLALMKEMGANAIRMSHNPHSLEMMQLCDEMGFIVQDEAFDVWKKKKVTNDYHKDWDAWHKQ DLEDFIKRDRNHPSVMMWSIGNEIREQFDSTGIAITRELAKIVKSLDKTRPVTSALTENVIEKNFIYQSGALDLLGFNYK HEDYKDFPTKFKGQKILASESVSALETRGHYDFPDGIKAWPTKHGAPFDGNADWTVSAYDQVKSYWGATHEENWKTIKSQ DFMAGTFIWTGFDYIGEPDPYPFPARSSYFGIVDLAGLPKDVYYMYQSEWSNKTVLHILPHWNWKKDQEVEVWAYYNNAD EVELFLNGKSLGKKAKQNDDLHISWRVKFEPGTLKAVSRKYGKVVLEKEIKTAGEASKIDLKADKTSIKNDTYDLVYVTV SMTDKDGNLVPNAMDLVNFEVSGGGKLVGVDNGYQANLDSFKANSCKLFNGKCVAIIQSNGKKENIQLKAMAGNGIPFSA IQINVN
Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]
COG id: COG3250
COG function: function code G; Beta-galactosidase/beta-glucuronidase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 2 family [H]
Homologues:
Organism=Homo sapiens, GI268834192, Length=460, Percent_Identity=24.1304347826087, Blast_Score=131, Evalue=3e-30, Organism=Escherichia coli, GI48994920, Length=453, Percent_Identity=25.1655629139073, Blast_Score=145, Evalue=7e-36, Organism=Escherichia coli, GI1787903, Length=465, Percent_Identity=26.0215053763441, Blast_Score=120, Evalue=5e-28, Organism=Escherichia coli, GI1786539, Length=456, Percent_Identity=24.780701754386, Blast_Score=117, Evalue=4e-27, Organism=Caenorhabditis elegans, GI17510825, Length=385, Percent_Identity=26.2337662337662, Blast_Score=104, Evalue=2e-22, Organism=Drosophila melanogaster, GI24655438, Length=426, Percent_Identity=29.81220657277, Blast_Score=159, Evalue=9e-39, Organism=Drosophila melanogaster, GI19922582, Length=426, Percent_Identity=29.81220657277, Blast_Score=159, Evalue=9e-39, Organism=Drosophila melanogaster, GI62471735, Length=426, Percent_Identity=29.81220657277, Blast_Score=159, Evalue=9e-39, Organism=Drosophila melanogaster, GI24651745, Length=582, Percent_Identity=24.0549828178694, Blast_Score=140, Evalue=4e-33,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003344 - InterPro: IPR008979 - InterPro: IPR006101 - InterPro: IPR013812 - InterPro: IPR006104 - InterPro: IPR006102 - InterPro: IPR006103 - InterPro: IPR017853 - InterPro: IPR013781 [H]
Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]
EC number: =3.2.1.23 [H]
Molecular weight: Translated: 91525; Mature: 91525
Theoretical pI: Translated: 8.73; Mature: 8.73
Prosite motif: PS00608 GLYCOSYL_HYDROL_F2_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 2.2 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 2.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIKHKNIFQKITIALLFSVSIFAQKQARIVEDFNKNWNFKLGDYPEAINANFTASDWRTL CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHCCCCEECCCCEEE QLPHDWSIEGAFDKDAKTKQAQGFLPAGKGWYRKVFTVPANWKNKTISIEFDGVFKNSEV ECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCEECCCEE FINGKSLGVRPNGYISFGYDLTQYLNFGKSNTIAVKVDNDAQPNSRWYTGSGIYRNVRLV EEECCEECCCCCCEEEECCCHHHHHCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEEE ASEKLHVGKWGTFVTTPEVSAEKSKIHLEVTIDNDNASAKEFKLVTSIINAENKEVVNFT EECCEEECCCCCEEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEC STEKIGAKTSEKKIHDLVLNQPKLWSTENPYLYKVITKVYEKSKLVDNYETPLGFRYFNF CCHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEC DSEKGFSLNGVPTKIYGVCLHHDNGALGAVENIHAVRRKLALMKEMGANAIRMSHNPHSL CCCCCCEECCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCH EMMQLCDEMGFIVQDEAFDVWKKKKVTNDYHKDWDAWHKQDLEDFIKRDRNHPSVMMWSI HHHHHHHHCCCEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC GNEIREQFDSTGIAITRELAKIVKSLDKTRPVTSALTENVIEKNFIYQSGALDLLGFNYK CHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEECCCEEEEECCCC HEDYKDFPTKFKGQKILASESVSALETRGHYDFPDGIKAWPTKHGAPFDGNADWTVSAYD CCCCCCCCCCCCCCEEEHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHH QVKSYWGATHEENWKTIKSQDFMAGTFIWTGFDYIGEPDPYPFPARSSYFGIVDLAGLPK HHHHHHCCCCCCCCCEECCCCCEEEEEEEECCHHCCCCCCCCCCCCCCCEEEEEECCCCH DVYYMYQSEWSNKTVLHILPHWNWKKDQEVEVWAYYNNADEVELFLNGKSLGKKAKQNDD HHEEEEECCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEEEEECCHHHCCCCCCCCC LHISWRVKFEPGTLKAVSRKYGKVVLEKEIKTAGEASKIDLKADKTSIKNDTYDLVYVTV EEEEEEEEECCCCHHHHHHHHCCEEEEHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEEE SMTDKDGNLVPNAMDLVNFEVSGGGKLVGVDNGYQANLDSFKANSCKLFNGKCVAIIQSN EEECCCCCCCCCCEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCEEEECCEEEEEEECC GKKENIQLKAMAGNGIPFSAIQINVN CCCCCEEEEEEECCCCCEEEEEEEEC >Mature Secondary Structure MIKHKNIFQKITIALLFSVSIFAQKQARIVEDFNKNWNFKLGDYPEAINANFTASDWRTL CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHCCCCEECCCCEEE QLPHDWSIEGAFDKDAKTKQAQGFLPAGKGWYRKVFTVPANWKNKTISIEFDGVFKNSEV ECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCEECCCEE FINGKSLGVRPNGYISFGYDLTQYLNFGKSNTIAVKVDNDAQPNSRWYTGSGIYRNVRLV EEECCEECCCCCCEEEECCCHHHHHCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEEE ASEKLHVGKWGTFVTTPEVSAEKSKIHLEVTIDNDNASAKEFKLVTSIINAENKEVVNFT EECCEEECCCCCEEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEC STEKIGAKTSEKKIHDLVLNQPKLWSTENPYLYKVITKVYEKSKLVDNYETPLGFRYFNF CCHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEC DSEKGFSLNGVPTKIYGVCLHHDNGALGAVENIHAVRRKLALMKEMGANAIRMSHNPHSL CCCCCCEECCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCH EMMQLCDEMGFIVQDEAFDVWKKKKVTNDYHKDWDAWHKQDLEDFIKRDRNHPSVMMWSI HHHHHHHHCCCEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC GNEIREQFDSTGIAITRELAKIVKSLDKTRPVTSALTENVIEKNFIYQSGALDLLGFNYK CHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEECCCEEEEECCCC HEDYKDFPTKFKGQKILASESVSALETRGHYDFPDGIKAWPTKHGAPFDGNADWTVSAYD CCCCCCCCCCCCCCEEEHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHH QVKSYWGATHEENWKTIKSQDFMAGTFIWTGFDYIGEPDPYPFPARSSYFGIVDLAGLPK HHHHHHCCCCCCCCCEECCCCCEEEEEEEECCHHCCCCCCCCCCCCCCCEEEEEECCCCH DVYYMYQSEWSNKTVLHILPHWNWKKDQEVEVWAYYNNADEVELFLNGKSLGKKAKQNDD HHEEEEECCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEEEEECCHHHCCCCCCCCC LHISWRVKFEPGTLKAVSRKYGKVVLEKEIKTAGEASKIDLKADKTSIKNDTYDLVYVTV EEEEEEEEECCCCHHHHHHHHCCEEEEHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEEE SMTDKDGNLVPNAMDLVNFEVSGGGKLVGVDNGYQANLDSFKANSCKLFNGKCVAIIQSN EEECCCCCCCCCCEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCEEEECCEEEEEEECC GKKENIQLKAMAGNGIPFSAIQINVN CCCCCEEEEEEECCCCCEEEEEEEEC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA