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The map label for this gene is yisY [H]

Identifier: 146301477

GI number: 146301477

Start: 4433761

End: 4434678

Strand: Reverse

Name: yisY [H]

Synonym: Fjoh_3736

Alternate gene names: 146301477

Gene position: 4434678-4433761 (Counterclockwise)

Preceding gene: 146301478

Following gene: 146301476

Centisome position: 72.74

GC content: 38.34

Gene sequence:

>918_bases
ATGAAAACCAATTTAAAAACAATCTTAACAACAAGCTTCGCCATTTTTTTAGCAGCAGTAACAACTGCATTCTCTCAAAG
CCAAAGAAAACCGGCAAGCCTTGGAAGAGAAGAATATATTGAAGTAGAAAAAAATGTAAAACTGCATGTAACGGATTTAG
GAGAAGGAAAACCGGTTGTACTAATTCACGGATGGCCATTGAGCGATGCGATGTACGAATACCAATATGCAGAACTTATC
CAGAAAGGATATCGCGTTATCGGAATCACCTTAAGAGGTTTTGGATTATCAGACAAACCGGGCGGGGCGTATAACTATGA
TGTTTATGCAGATGATATCAAAGTGATTCTGGACAAACTTAAAATTGAAAATGCAACTATCGGTGGATTCTCTATGGGAG
GTGCGACAGTTATTCACTATGCAGCAAAATACAATGCGGCCCATGTTTCAAAACTGGCATTGTTTGGTGCGGCAGCGCCT
TTATGGACAAAAAGAGCTGACTTTAACTATGGTTTCTGGACAAAAGAAGATGTAAATGGTCTTATTGCCCTTAATAATAC
CAACAGACCACAATTGTTTGAGAATTTTGGAAAAATTTTCCCGGCCAATGAAACAAGCGTATCTGCAGGACAAGGTGCAT
GGCTGGGAACTATTCAGGCGCAGGCTTCTCCCTATGCAATGGCTGAATCTATGAAAGCACTTCGTGATACAGATTTAAGA
GAAGATTTGAAAAAAATCAAAATTCCAACTCTTATTCTTCACGGAACACAGGACAAAATTTGTTCTTACGAATTAGCTGA
GCAAATGCACAAATCAATTAGCAATTCTACTTTGGTTCCATTCGAGAAAAGCGGTCATGCCTTGTTTATTGAAGAGCTTC
CAAAATTTAATGCCGAATTGATCAAATTCATAAAATAA

Upstream 100 bases:

>100_bases
ATTATTAATCTGATTTATAATTAAAGAAACCGCAATTTTGTCCGGTCAATAAGAAATAAAAAAAATAAAAACTTTAAATA
ATAATTAAAACACAAAAGAA

Downstream 100 bases:

>100_bases
GTAAAAACAAAATCAGGAAAGCTGTCTTTTAAAGACGGCTTTCTTTTTTAGATAATTATAAGAGGGAAATAATGAAAAAT
ATAGTAATGCTTGCATTAGG

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKTNLKTILTTSFAIFLAAVTTAFSQSQRKPASLGREEYIEVEKNVKLHVTDLGEGKPVVLIHGWPLSDAMYEYQYAELI
QKGYRVIGITLRGFGLSDKPGGAYNYDVYADDIKVILDKLKIENATIGGFSMGGATVIHYAAKYNAAHVSKLALFGAAAP
LWTKRADFNYGFWTKEDVNGLIALNNTNRPQLFENFGKIFPANETSVSAGQGAWLGTIQAQASPYAMAESMKALRDTDLR
EDLKKIKIPTLILHGTQDKICSYELAEQMHKSISNSTLVPFEKSGHALFIEELPKFNAELIKFIK

Sequences:

>Translated_305_residues
MKTNLKTILTTSFAIFLAAVTTAFSQSQRKPASLGREEYIEVEKNVKLHVTDLGEGKPVVLIHGWPLSDAMYEYQYAELI
QKGYRVIGITLRGFGLSDKPGGAYNYDVYADDIKVILDKLKIENATIGGFSMGGATVIHYAAKYNAAHVSKLALFGAAAP
LWTKRADFNYGFWTKEDVNGLIALNNTNRPQLFENFGKIFPANETSVSAGQGAWLGTIQAQASPYAMAESMKALRDTDLR
EDLKKIKIPTLILHGTQDKICSYELAEQMHKSISNSTLVPFEKSGHALFIEELPKFNAELIKFIK
>Mature_305_residues
MKTNLKTILTTSFAIFLAAVTTAFSQSQRKPASLGREEYIEVEKNVKLHVTDLGEGKPVVLIHGWPLSDAMYEYQYAELI
QKGYRVIGITLRGFGLSDKPGGAYNYDVYADDIKVILDKLKIENATIGGFSMGGATVIHYAAKYNAAHVSKLALFGAAAP
LWTKRADFNYGFWTKEDVNGLIALNNTNRPQLFENFGKIFPANETSVSAGQGAWLGTIQAQASPYAMAESMKALRDTDLR
EDLKKIKIPTLILHGTQDKICSYELAEQMHKSISNSTLVPFEKSGHALFIEELPKFNAELIKFIK

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily [H]

Homologues:

Organism=Homo sapiens, GI27597073, Length=307, Percent_Identity=23.4527687296417, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI87081721, Length=280, Percent_Identity=24.6428571428571, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 33794; Mature: 33794

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNLKTILTTSFAIFLAAVTTAFSQSQRKPASLGREEYIEVEKNVKLHVTDLGEGKPVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHEEECCCCEEEEEECCCCCCEE
LIHGWPLSDAMYEYQYAELIQKGYRVIGITLRGFGLSDKPGGAYNYDVYADDIKVILDKL
EEECCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCEECCEEHHHHHHHHHHH
KIENATIGGFSMGGATVIHYAAKYNAAHVSKLALFGAAAPLWTKRADFNYGFWTKEDVNG
HHCCCEECCCCCCCHHEEEEHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCE
LIALNNTNRPQLFENFGKIFPANETSVSAGQGAWLGTIQAQASPYAMAESMKALRDTDLR
EEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHH
EDLKKIKIPTLILHGTQDKICSYELAEQMHKSISNSTLVPFEKSGHALFIEELPKFNAEL
HHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEECEEECCCCEEEHHHCCCCCHHH
IKFIK
HHHCC
>Mature Secondary Structure
MKTNLKTILTTSFAIFLAAVTTAFSQSQRKPASLGREEYIEVEKNVKLHVTDLGEGKPVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHEEECCCCEEEEEECCCCCCEE
LIHGWPLSDAMYEYQYAELIQKGYRVIGITLRGFGLSDKPGGAYNYDVYADDIKVILDKL
EEECCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCEECCEEHHHHHHHHHHH
KIENATIGGFSMGGATVIHYAAKYNAAHVSKLALFGAAAPLWTKRADFNYGFWTKEDVNG
HHCCCEECCCCCCCHHEEEEHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCE
LIALNNTNRPQLFENFGKIFPANETSVSAGQGAWLGTIQAQASPYAMAESMKALRDTDLR
EEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHH
EDLKKIKIPTLILHGTQDKICSYELAEQMHKSISNSTLVPFEKSGHALFIEELPKFNAEL
HHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEECEEECCCCEEEHHHCCCCCHHH
IKFIK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377 [H]