Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 146301217

GI number: 146301217

Start: 4140568

End: 4142574

Strand: Reverse

Name: ligA

Synonym: Fjoh_3475

Alternate gene names: 146301217

Gene position: 4142574-4140568 (Counterclockwise)

Preceding gene: 146301218

Following gene: 146301216

Centisome position: 67.95

GC content: 35.28

Gene sequence:

>2007_bases
ATGAATATTCAGGAAACCATTCAAACATTAAGAGAAGAACTTAATCAGCACAATTACAATTACTATGTGCTGGATAACGC
CACAATTTCTGATTACGATTTTGATATCAAATTAAAAGAACTGCAGGATTTAGAAAACAAACATCCTGAATTTTTTGACG
AAGATTCACCAACACAAAGAGTCGGCGGAACAGTAACAAAAAACTTCAAAACCATAGCGCACCAATACAGAATGTATTCG
CTTGATAATTCCTATTCTAAAGAAGATCTAATAGATTGGGAAAACAGAATTCAGCGTGTATTAGGTAACGTTAATTTACA
ATATACCTGCGAATTAAAATACGACGGAGCCTCAATCAGCATTTCATACCAAAACGGAAAATTAGTTCAGGCACTTACAC
GCGGCGATGGTTTTCAAGGAGATGAGGTTACCAATAATATCAAAACCATCAAATCTATTCCGTTAAAATTAAAAGGAGAT
TATCCAGAAAGATTTGATATACGAGGAGAAATAATCCTTCCTTTTGCCGGTTTCGAAAAAATGAATCAGGAACTAATCGA
AATTGGAGAAACCCCATATTCAAACCCAAGAAATACAGCTTCCGGAAGTTTAAAATTACAAGACAGTGCAGAAGTGGCAA
AACGTCCTCTTGAATGTCTTTTATATACTGTTGCAGGAAATAATCTTCCTTTTAAAACTCAATTTGAAGGATTAGAATCG
GCCAGAAAATGGGGATTCAAAGTGCCAAATGAAGCCAAACTGGTAAACAGTATGCAGGAAGTTTTTGATTTTATTGACTA
TTGGGATGTTCATCGCCATAAATTACCTTATGAGACTGACGGTGTTGTTATAAAAGTAAACAGCATTCATTCTCAGGAAG
AACTTGGATACACGGCAAAATCACCACGCTGGGCCATTGCCTACAAATTCAAATCAGAACAGGCTTCAACAAAATTAAAA
TCAATATCGTATCAGGTTGGGCGTACAGGAGCCATTACTCCAGTTGCCAATCTAGAACCTGTGCAGCTGGCAGGAACTAT
TGTAAAAAGAGCTTCACTTCACAATGCAGATCAAATTGAAAAATTAGACATTAGAATAAATGATACCGTTTTTGTTGAAA
AAGGCGGAGAAATTATTCCTAAAATTATTGCCGTAGATCTGGAAAAACGTCCTGAAAATTCAGAAGTTACACAATACATA
ACACATTGTCCTGAATGCGAGACTGAATTGGTTCGAAATGCTGGAGAAGCCAACCATTACTGCCCGAATTTTTATGGATG
CCCACCGCAGATTATCGGAAGAATCCAGCATTATATTTCAAGAAAAGCAATGGATATTGAAGGACTTGGAGGAGAAACCG
TTGCTTTATTATTCAAAAACGGCTTAGTTCACAACTATGCAGATTTATATGAATTAAAAGTAGAAGATATTCTTCATTTA
GAAAGAATGGCACAAAAATCTGCCGAAAATTTGGTAAACGGTGTAGAAAAATCAAAAGAAATTCCATTTGAAAGCGTTCT
TTTTGCGCTTGGAATTCGTTTTGTTGGAGAAACCGTAGCCAAAAAGCTAGCCAAACATTATAAAAACATAGACGCTTTAA
GCCAGGCTTCATTAATGGATTTAATTCTGGTTGATGAAATTGGAGAAAGAATTGCCAGAAGCGTTATCGAATTTTTCGAA
AACGAAGAAAATAAAATCATAATCGAACGATTAAAAAAATACGGTATTCAGTTTGAAACCGTAGAAAGAGTCAACCCAAA
TGCTACCGAGAAATTTATTGGAAAAACTTTTGTAGTTTCAGGAGTTTTCAGCCAATTTTCAAGAGATGAATTAAAGAAAG
CCATCGAAGACAACGGAGGAAAAGTAGGAAGTTCAATTTCTGCAAAAACCGACTTTGTGGTAGCAGGAGATAATATGGGA
CCAGCTAAATTAGAAAAAGCAACAAAATTAAATATTCCTATACTATCTGAAGACGAATTTATAACCAAACTTAATGAAAG
CGAATAA

Upstream 100 bases:

>100_bases
TTTTATAAAACTTTCAAATTTTAATGTTTTTGAATTAAAATAATCCTAATTTTGATATTCAAATTACTTTCAAATCAAAT
CAGCATTATTTTAATATTAT

Downstream 100 bases:

>100_bases
GCCATCGTTGATTTTATTTTTCTTGTCATTACTGTTTACAATCATATTTGATTGTACTAAACAGGACTTCATGGCTATCT
ATGCCAAAGCAATAGTATTA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MNIQETIQTLREELNQHNYNYYVLDNATISDYDFDIKLKELQDLENKHPEFFDEDSPTQRVGGTVTKNFKTIAHQYRMYS
LDNSYSKEDLIDWENRIQRVLGNVNLQYTCELKYDGASISISYQNGKLVQALTRGDGFQGDEVTNNIKTIKSIPLKLKGD
YPERFDIRGEIILPFAGFEKMNQELIEIGETPYSNPRNTASGSLKLQDSAEVAKRPLECLLYTVAGNNLPFKTQFEGLES
ARKWGFKVPNEAKLVNSMQEVFDFIDYWDVHRHKLPYETDGVVIKVNSIHSQEELGYTAKSPRWAIAYKFKSEQASTKLK
SISYQVGRTGAITPVANLEPVQLAGTIVKRASLHNADQIEKLDIRINDTVFVEKGGEIIPKIIAVDLEKRPENSEVTQYI
THCPECETELVRNAGEANHYCPNFYGCPPQIIGRIQHYISRKAMDIEGLGGETVALLFKNGLVHNYADLYELKVEDILHL
ERMAQKSAENLVNGVEKSKEIPFESVLFALGIRFVGETVAKKLAKHYKNIDALSQASLMDLILVDEIGERIARSVIEFFE
NEENKIIIERLKKYGIQFETVERVNPNATEKFIGKTFVVSGVFSQFSRDELKKAIEDNGGKVGSSISAKTDFVVAGDNMG
PAKLEKATKLNIPILSEDEFITKLNESE

Sequences:

>Translated_668_residues
MNIQETIQTLREELNQHNYNYYVLDNATISDYDFDIKLKELQDLENKHPEFFDEDSPTQRVGGTVTKNFKTIAHQYRMYS
LDNSYSKEDLIDWENRIQRVLGNVNLQYTCELKYDGASISISYQNGKLVQALTRGDGFQGDEVTNNIKTIKSIPLKLKGD
YPERFDIRGEIILPFAGFEKMNQELIEIGETPYSNPRNTASGSLKLQDSAEVAKRPLECLLYTVAGNNLPFKTQFEGLES
ARKWGFKVPNEAKLVNSMQEVFDFIDYWDVHRHKLPYETDGVVIKVNSIHSQEELGYTAKSPRWAIAYKFKSEQASTKLK
SISYQVGRTGAITPVANLEPVQLAGTIVKRASLHNADQIEKLDIRINDTVFVEKGGEIIPKIIAVDLEKRPENSEVTQYI
THCPECETELVRNAGEANHYCPNFYGCPPQIIGRIQHYISRKAMDIEGLGGETVALLFKNGLVHNYADLYELKVEDILHL
ERMAQKSAENLVNGVEKSKEIPFESVLFALGIRFVGETVAKKLAKHYKNIDALSQASLMDLILVDEIGERIARSVIEFFE
NEENKIIIERLKKYGIQFETVERVNPNATEKFIGKTFVVSGVFSQFSRDELKKAIEDNGGKVGSSISAKTDFVVAGDNMG
PAKLEKATKLNIPILSEDEFITKLNESE
>Mature_668_residues
MNIQETIQTLREELNQHNYNYYVLDNATISDYDFDIKLKELQDLENKHPEFFDEDSPTQRVGGTVTKNFKTIAHQYRMYS
LDNSYSKEDLIDWENRIQRVLGNVNLQYTCELKYDGASISISYQNGKLVQALTRGDGFQGDEVTNNIKTIKSIPLKLKGD
YPERFDIRGEIILPFAGFEKMNQELIEIGETPYSNPRNTASGSLKLQDSAEVAKRPLECLLYTVAGNNLPFKTQFEGLES
ARKWGFKVPNEAKLVNSMQEVFDFIDYWDVHRHKLPYETDGVVIKVNSIHSQEELGYTAKSPRWAIAYKFKSEQASTKLK
SISYQVGRTGAITPVANLEPVQLAGTIVKRASLHNADQIEKLDIRINDTVFVEKGGEIIPKIIAVDLEKRPENSEVTQYI
THCPECETELVRNAGEANHYCPNFYGCPPQIIGRIQHYISRKAMDIEGLGGETVALLFKNGLVHNYADLYELKVEDILHL
ERMAQKSAENLVNGVEKSKEIPFESVLFALGIRFVGETVAKKLAKHYKNIDALSQASLMDLILVDEIGERIARSVIEFFE
NEENKIIIERLKKYGIQFETVERVNPNATEKFIGKTFVVSGVFSQFSRDELKKAIEDNGGKVGSSISAKTDFVVAGDNMG
PAKLEKATKLNIPILSEDEFITKLNESE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=670, Percent_Identity=42.2388059701493, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI87082305, Length=454, Percent_Identity=22.4669603524229, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_FLAJ1 (A5FE87)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001195808.1
- ProteinModelPortal:   A5FE87
- STRING:   A5FE87
- GeneID:   5089676
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_3475
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK2533533
- BioCyc:   FJOH376686:FJOH_3475-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75663; Mature: 75663

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 113-113 BINDING 111-111 BINDING 134-134 BINDING 170-170 BINDING 285-285 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQETIQTLREELNQHNYNYYVLDNATISDYDFDIKLKELQDLENKHPEFFDEDSPTQR
CCHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCEEHHHHHHHHHCCCCCCCCCCCHHH
VGGTVTKNFKTIAHQYRMYSLDNSYSKEDLIDWENRIQRVLGNVNLQYTCELKYDGASIS
CCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEE
ISYQNGKLVQALTRGDGFQGDEVTNNIKTIKSIPLKLKGDYPERFDIRGEIILPFAGFEK
EEECCCCEEHHHHCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEECCHHH
MNQELIEIGETPYSNPRNTASGSLKLQDSAEVAKRPLECLLYTVAGNNLPFKTQFEGLES
HHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHCCHHHEEEEEECCCCCCCHHHHHHHH
ARKWGFKVPNEAKLVNSMQEVFDFIDYWDVHRHKLPYETDGVVIKVNSIHSQEELGYTAK
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHCCCCCC
SPRWAIAYKFKSEQASTKLKSISYQVGRTGAITPVANLEPVQLAGTIVKRASLHNADQIE
CCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCE
KLDIRINDTVFVEKGGEIIPKIIAVDLEKRPENSEVTQYITHCPECETELVRNAGEANHY
EEEEEECCEEEEECCCCCCCHHHEEECCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCC
CPNFYGCPPQIIGRIQHYISRKAMDIEGLGGETVALLFKNGLVHNYADLYELKVEDILHL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHCCCHHHHHHHHHHHHHHHHHH
ERMAQKSAENLVNGVEKSKEIPFESVLFALGIRFVGETVAKKLAKHYKNIDALSQASLMD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
LILVDEIGERIARSVIEFFENEENKIIIERLKKYGIQFETVERVNPNATEKFIGKTFVVS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEHHCCCCCHHHHHHHHHHHHH
GVFSQFSRDELKKAIEDNGGKVGSSISAKTDFVVAGDNMGPAKLEKATKLNIPILSEDEF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCEEECCCHH
ITKLNESE
HHHCCCCC
>Mature Secondary Structure
MNIQETIQTLREELNQHNYNYYVLDNATISDYDFDIKLKELQDLENKHPEFFDEDSPTQR
CCHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCEEHHHHHHHHHCCCCCCCCCCCHHH
VGGTVTKNFKTIAHQYRMYSLDNSYSKEDLIDWENRIQRVLGNVNLQYTCELKYDGASIS
CCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEE
ISYQNGKLVQALTRGDGFQGDEVTNNIKTIKSIPLKLKGDYPERFDIRGEIILPFAGFEK
EEECCCCEEHHHHCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEECCHHH
MNQELIEIGETPYSNPRNTASGSLKLQDSAEVAKRPLECLLYTVAGNNLPFKTQFEGLES
HHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHCCHHHEEEEEECCCCCCCHHHHHHHH
ARKWGFKVPNEAKLVNSMQEVFDFIDYWDVHRHKLPYETDGVVIKVNSIHSQEELGYTAK
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHCCCCCC
SPRWAIAYKFKSEQASTKLKSISYQVGRTGAITPVANLEPVQLAGTIVKRASLHNADQIE
CCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCE
KLDIRINDTVFVEKGGEIIPKIIAVDLEKRPENSEVTQYITHCPECETELVRNAGEANHY
EEEEEECCEEEEECCCCCCCHHHEEECCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCC
CPNFYGCPPQIIGRIQHYISRKAMDIEGLGGETVALLFKNGLVHNYADLYELKVEDILHL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHCCCHHHHHHHHHHHHHHHHHH
ERMAQKSAENLVNGVEKSKEIPFESVLFALGIRFVGETVAKKLAKHYKNIDALSQASLMD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
LILVDEIGERIARSVIEFFENEENKIIIERLKKYGIQFETVERVNPNATEKFIGKTFVVS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEHHCCCCCHHHHHHHHHHHHH
GVFSQFSRDELKKAIEDNGGKVGSSISAKTDFVVAGDNMGPAKLEKATKLNIPILSEDEF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCEEECCCHH
ITKLNESE
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA