Click here to switch to the map view.

The map label for this gene is prfH [H]

Identifier: 146301198

GI number: 146301198

Start: 4122084

End: 4122773

Strand: Reverse

Name: prfH [H]

Synonym: Fjoh_3456

Alternate gene names: 146301198

Gene position: 4122773-4122084 (Counterclockwise)

Preceding gene: 146301199

Following gene: 146301197

Centisome position: 67.62

GC content: 38.12

Gene sequence:

>690_bases
ATGGAAAGAATAATTCAGATAACAGCGGGTCGCGGACCTGCAGAATGCACTTGGGTTGTCGCTCAAGTGCTTAAAAAAGT
TTTGGAAGAAGCACAAGATCAGAATTTAGATGTGGTTTTACTGCAAAGAGAGAAAGGAGAAGAGAACGGAACAATTGAAA
CCGCATCGATTTCTGTAAAGGGAAAAAATGCAGATCAATTTGTGAAATCTTGGATAGGAACGATTCAATGGATTGGGCAG
AGTCAGTTTAGGAAAATGCATAAACGCAAAAACTGGTTTATTGGGATTTTTGAAATCGAACCGCAAAAAAATGCATCAGT
TTCAGAAAACGACATTCAATATCAGGCGATGCGAAGTTCCGGCGCCGGCGGTCAGCATGTAAACAAAGTGAGTTCAGCAA
TTCGTGCAACACATATTCCAACAGGAATTGCAGTTGTGGCAATGGACAGCCGTTCGCAGCACCAAAACAAAAAACTGGCT
ACAGAAAGATTATTAAAAAAATTAGAAGACGAGAAACTAGTTCAGCTTAAAAATCACGTAGGAAAGCAATGGGAAAATCA
GCTTAATGTAGAGCGTGGAAATCCAATTAGAGTTTTTACAGGAAGTGATTTTAAAAAGATTAAAGTTGAGAAAAGCTACA
AAGGAACTCGTCAGAAATTAAAAAACGATTTACGAAATGAAAACAATTGA

Upstream 100 bases:

>100_bases
GAAACAATGATGGGAGTGGTAATGGAACTATGGTTTCGAGACTCCCACAATTTGTAAAAGCAGCCAAAAACAGAGAAAAG
CTGCTCAAAATTTTAAAGAA

Downstream 100 bases:

>100_bases
TAAATACCTTTTTCAAGCCTTGGATAATTATCCGTTTTGGCTTGAAGGAACAATAGAATCTTTAGATTATGCTTTTTCTT
ATGACGATAAAAACACGATG

Product: peptide chain release factor-like protein

Products: NA

Alternate protein names: RF-H [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MERIIQITAGRGPAECTWVVAQVLKKVLEEAQDQNLDVVLLQREKGEENGTIETASISVKGKNADQFVKSWIGTIQWIGQ
SQFRKMHKRKNWFIGIFEIEPQKNASVSENDIQYQAMRSSGAGGQHVNKVSSAIRATHIPTGIAVVAMDSRSQHQNKKLA
TERLLKKLEDEKLVQLKNHVGKQWENQLNVERGNPIRVFTGSDFKKIKVEKSYKGTRQKLKNDLRNENN

Sequences:

>Translated_229_residues
MERIIQITAGRGPAECTWVVAQVLKKVLEEAQDQNLDVVLLQREKGEENGTIETASISVKGKNADQFVKSWIGTIQWIGQ
SQFRKMHKRKNWFIGIFEIEPQKNASVSENDIQYQAMRSSGAGGQHVNKVSSAIRATHIPTGIAVVAMDSRSQHQNKKLA
TERLLKKLEDEKLVQLKNHVGKQWENQLNVERGNPIRVFTGSDFKKIKVEKSYKGTRQKLKNDLRNENN
>Mature_229_residues
MERIIQITAGRGPAECTWVVAQVLKKVLEEAQDQNLDVVLLQREKGEENGTIETASISVKGKNADQFVKSWIGTIQWIGQ
SQFRKMHKRKNWFIGIFEIEPQKNASVSENDIQYQAMRSSGAGGQHVNKVSSAIRATHIPTGIAVVAMDSRSQHQNKKLA
TERLLKKLEDEKLVQLKNHVGKQWENQLNVERGNPIRVFTGSDFKKIKVEKSYKGTRQKLKNDLRNENN

Specific function: Could be involved in translational termination [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=167, Percent_Identity=28.7425149700599, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI2367172, Length=203, Percent_Identity=30.0492610837438, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1787462, Length=159, Percent_Identity=30.8176100628931, Blast_Score=73, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321295, Length=58, Percent_Identity=53.448275862069, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921226, Length=131, Percent_Identity=32.0610687022901, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000352
- InterPro:   IPR017509 [H]

Pfam domain/function: PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 26031; Mature: 26031

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIIQITAGRGPAECTWVVAQVLKKVLEEAQDQNLDVVLLQREKGEENGTIETASISVK
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEEE
GKNADQFVKSWIGTIQWIGQSQFRKMHKRKNWFIGIFEIEPQKNASVSENDIQYQAMRSS
CCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCC
GAGGQHVNKVSSAIRATHIPTGIAVVAMDSRSQHQNKKLATERLLKKLEDEKLVQLKNHV
CCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
GKQWENQLNVERGNPIRVFTGSDFKKIKVEKSYKGTRQKLKNDLRNENN
HHHHHHHCCCCCCCEEEEEECCCCEEEEEECHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MERIIQITAGRGPAECTWVVAQVLKKVLEEAQDQNLDVVLLQREKGEENGTIETASISVK
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEEE
GKNADQFVKSWIGTIQWIGQSQFRKMHKRKNWFIGIFEIEPQKNASVSENDIQYQAMRSS
CCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCC
GAGGQHVNKVSSAIRATHIPTGIAVVAMDSRSQHQNKKLATERLLKKLEDEKLVQLKNHV
CCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
GKQWENQLNVERGNPIRVFTGSDFKKIKVEKSYKGTRQKLKNDLRNENN
HHHHHHHCCCCCCCEEEEEECCCCEEEEEECHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1695895; 9278503; 1408743 [H]