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The map label for this gene is emrB [H]

Identifier: 146301119

GI number: 146301119

Start: 4034534

End: 4036084

Strand: Direct

Name: emrB [H]

Synonym: Fjoh_3377

Alternate gene names: 146301119

Gene position: 4034534-4036084 (Clockwise)

Preceding gene: 146301118

Following gene: 146301121

Centisome position: 66.17

GC content: 38.36

Gene sequence:

>1551_bases
ATGACACCTTTAAACCGTAACCTGCTCATTATTACAGTCATTCTTGCTGCCATTATGGAACTAATTGATATTTCTATCGT
AAATGTGGCGCTTTCGCACATGAGCGGTAATCTGGGTGCAACTCTCGAAGATACTTCGTGGGTAATTACTGCTTATGCCA
TTGCAAATGTTATTATTATTCCCATAACGAGCTTTTTAAGCGCCAATCTGGGCAGACGCAATTATTATATTGGTTCTGTT
GTTTTATTTACATTCTGCTCTTTTATGTGCGGCCACTCGTCTAACATCTGGATGCTGGTCTTTTTTAGATTTTTTCAGGG
AATTGGCGGCGGTGCTTTATTATCTATCTCACAGGTTGTTGTATTTGAACTTTTTCCAAAAGACAAACAATCAACAGCCG
GAGCTTTGTTTGGAGCCGGAATTTTTATCGGCCCTACAATTGGGCCGACACTTGGCGGCTATATTACTGAAAATTATGAC
TGGCCGTGGATTTTTTTAATTAATATTCCTATTGGAATTTTGGTTACGATTTCTTGTTATTTCCTTTTACGCGAACCCAC
TGTAAAGCCAGAAATTACCAAAGTCGACTGGACCGGAATTGCGCTTCTCGCTATAGGAGTGGGCGCTTTACAAACGGTAT
TAGAAAGAGGCGAAGTCGAAGACTGGTTCGAAACCAAATATATTGTTGTACTAACGATTATTGCAGTAACAACTTTACTC
GCCTTTATTTATTGGGAATTAACAATAGAAAAACCAGTCGTAAACCTTCGGGTGATTAAAAGCAAATCGTTAAGCATTGC
AGCAATTTTGACTTTTGTAACCGGTTTTGGAATGTTTATTTCCATTTATATAAGTCCGGTTGTGGCACAGCGTTTATTGA
GTTTTTCACCGTATCAAACAGGATTACTTTTGCTTCCGGGTGCATTATTTGCTTTACTGGCTTTAAAAATCTGCGGTACA
TTATTACAAAAAGGCGTTTCTCCGGTTATTATTATTGCTGCTGGATTTCTGTTATTTATCTATTTCAATTGGCGAATGTC
TGGTATGACTTTAAACACAAGCGCTGCCGAAGTCGCTACATCTCTTGTTTTTCGTGCTTTAGGAATGGCGCTGCTTACCG
TACCGCTTACTATGCTGGCTGTTTCTTCATTAGATGATAAAGATGTTGGGCAAGGTGCTGCGCTTAATAATATGATGAGA
CAATTGGGCGGTTCATTTGGTGTATCGATCATCAATACTTATGTGGCTCATCGATTTGCCGCACATCGAAATGAATTGAT
TTCAGAGGTGACACCAAGTAATACTCTCGCTATGGATCGCATAAATGCTTACATAAAGTACTTTCAATATAGAGGTTTTA
CTTATAATGAAGCCAGAGCAAAAGCCTTAAAGCTGATGGAAAATATCGTAATGAAACAATCTTCGCTGTTAAGTTACAGA
GACGCTTTTTTTCTTGTTGGTTTGTTTTTTGTTGTAACGCTGCCCCTTCTTTTATTTGTAATGAACAAATCGAAAAAGCC
AAAAACCAACATGGTAATTTCCGATCATTAA

Upstream 100 bases:

>100_bases
TTAAAACAAATGTGGTAAAAAAGGTGCTGTCTTAAAAAGCACCTGATTTCTCATCCTCTTTTTTTGAACAAAAATATACC
AATTGGTATAAAATATTTCT

Downstream 100 bases:

>100_bases
TTTTTTTCCTGCAGATTTTAAAAATCTGCAGGGCTTTATCTATTTCTTCAAAATTCCTGTTTTTAATCCTTTGGCGAAAG
CCGATAAAACAACTTCATCT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTPLNRNLLIITVILAAIMELIDISIVNVALSHMSGNLGATLEDTSWVITAYAIANVIIIPITSFLSANLGRRNYYIGSV
VLFTFCSFMCGHSSNIWMLVFFRFFQGIGGGALLSISQVVVFELFPKDKQSTAGALFGAGIFIGPTIGPTLGGYITENYD
WPWIFLINIPIGILVTISCYFLLREPTVKPEITKVDWTGIALLAIGVGALQTVLERGEVEDWFETKYIVVLTIIAVTTLL
AFIYWELTIEKPVVNLRVIKSKSLSIAAILTFVTGFGMFISIYISPVVAQRLLSFSPYQTGLLLLPGALFALLALKICGT
LLQKGVSPVIIIAAGFLLFIYFNWRMSGMTLNTSAAEVATSLVFRALGMALLTVPLTMLAVSSLDDKDVGQGAALNNMMR
QLGGSFGVSIINTYVAHRFAAHRNELISEVTPSNTLAMDRINAYIKYFQYRGFTYNEARAKALKLMENIVMKQSSLLSYR
DAFFLVGLFFVVTLPLLLFVMNKSKKPKTNMVISDH

Sequences:

>Translated_516_residues
MTPLNRNLLIITVILAAIMELIDISIVNVALSHMSGNLGATLEDTSWVITAYAIANVIIIPITSFLSANLGRRNYYIGSV
VLFTFCSFMCGHSSNIWMLVFFRFFQGIGGGALLSISQVVVFELFPKDKQSTAGALFGAGIFIGPTIGPTLGGYITENYD
WPWIFLINIPIGILVTISCYFLLREPTVKPEITKVDWTGIALLAIGVGALQTVLERGEVEDWFETKYIVVLTIIAVTTLL
AFIYWELTIEKPVVNLRVIKSKSLSIAAILTFVTGFGMFISIYISPVVAQRLLSFSPYQTGLLLLPGALFALLALKICGT
LLQKGVSPVIIIAAGFLLFIYFNWRMSGMTLNTSAAEVATSLVFRALGMALLTVPLTMLAVSSLDDKDVGQGAALNNMMR
QLGGSFGVSIINTYVAHRFAAHRNELISEVTPSNTLAMDRINAYIKYFQYRGFTYNEARAKALKLMENIVMKQSSLLSYR
DAFFLVGLFFVVTLPLLLFVMNKSKKPKTNMVISDH
>Mature_515_residues
TPLNRNLLIITVILAAIMELIDISIVNVALSHMSGNLGATLEDTSWVITAYAIANVIIIPITSFLSANLGRRNYYIGSVV
LFTFCSFMCGHSSNIWMLVFFRFFQGIGGGALLSISQVVVFELFPKDKQSTAGALFGAGIFIGPTIGPTLGGYITENYDW
PWIFLINIPIGILVTISCYFLLREPTVKPEITKVDWTGIALLAIGVGALQTVLERGEVEDWFETKYIVVLTIIAVTTLLA
FIYWELTIEKPVVNLRVIKSKSLSIAAILTFVTGFGMFISIYISPVVAQRLLSFSPYQTGLLLLPGALFALLALKICGTL
LQKGVSPVIIIAAGFLLFIYFNWRMSGMTLNTSAAEVATSLVFRALGMALLTVPLTMLAVSSLDDKDVGQGAALNNMMRQ
LGGSFGVSIINTYVAHRFAAHRNELISEVTPSNTLAMDRINAYIKYFQYRGFTYNEARAKALKLMENIVMKQSSLLSYRD
AFFLVGLFFVVTLPLLLFVMNKSKKPKTNMVISDH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=500, Percent_Identity=32, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1788710, Length=502, Percent_Identity=30.4780876494024, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1790195, Length=403, Percent_Identity=25.0620347394541, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87081983, Length=408, Percent_Identity=25.4901960784314, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1788392, Length=288, Percent_Identity=28.8194444444444, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319770, Length=296, Percent_Identity=26.3513513513513, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322958, Length=496, Percent_Identity=22.1774193548387, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=26, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56790; Mature: 56659

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLNRNLLIITVILAAIMELIDISIVNVALSHMSGNLGATLEDTSWVITAYAIANVIII
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHH
PITSFLSANLGRRNYYIGSVVLFTFCSFMCGHSSNIWMLVFFRFFQGIGGGALLSISQVV
HHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
VFELFPKDKQSTAGALFGAGIFIGPTIGPTLGGYITENYDWPWIFLINIPIGILVTISCY
HHHCCCCCCCCCCHHHHCCCEEECCCCCCCCCCCEECCCCCCEEEEEECCHHHHHHHHHH
FLLREPTVKPEITKVDWTGIALLAIGVGALQTVLERGEVEDWFETKYIVVLTIIAVTTLL
HHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AFIYWELTIEKPVVNLRVIKSKSLSIAAILTFVTGFGMFISIYISPVVAQRLLSFSPYQT
HHHHHHHEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GLLLLPGALFALLALKICGTLLQKGVSPVIIIAAGFLLFIYFNWRMSGMTLNTSAAEVAT
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEECEEECCHHHHHHH
SLVFRALGMALLTVPLTMLAVSSLDDKDVGQGAALNNMMRQLGGSFGVSIINTYVAHRFA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AHRNELISEVTPSNTLAMDRINAYIKYFQYRGFTYNEARAKALKLMENIVMKQSSLLSYR
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DAFFLVGLFFVVTLPLLLFVMNKSKKPKTNMVISDH
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECC
>Mature Secondary Structure 
TPLNRNLLIITVILAAIMELIDISIVNVALSHMSGNLGATLEDTSWVITAYAIANVIII
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHH
PITSFLSANLGRRNYYIGSVVLFTFCSFMCGHSSNIWMLVFFRFFQGIGGGALLSISQVV
HHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
VFELFPKDKQSTAGALFGAGIFIGPTIGPTLGGYITENYDWPWIFLINIPIGILVTISCY
HHHCCCCCCCCCCHHHHCCCEEECCCCCCCCCCCEECCCCCCEEEEEECCHHHHHHHHHH
FLLREPTVKPEITKVDWTGIALLAIGVGALQTVLERGEVEDWFETKYIVVLTIIAVTTLL
HHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AFIYWELTIEKPVVNLRVIKSKSLSIAAILTFVTGFGMFISIYISPVVAQRLLSFSPYQT
HHHHHHHEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GLLLLPGALFALLALKICGTLLQKGVSPVIIIAAGFLLFIYFNWRMSGMTLNTSAAEVAT
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEECEEECCHHHHHHH
SLVFRALGMALLTVPLTMLAVSSLDDKDVGQGAALNNMMRQLGGSFGVSIINTYVAHRFA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AHRNELISEVTPSNTLAMDRINAYIKYFQYRGFTYNEARAKALKLMENIVMKQSSLLSYR
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DAFFLVGLFFVVTLPLLLFVMNKSKKPKTNMVISDH
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]