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The map label for this gene is azoR [H]

Identifier: 146301066

GI number: 146301066

Start: 3969343

End: 3969954

Strand: Reverse

Name: azoR [H]

Synonym: Fjoh_3322

Alternate gene names: 146301066

Gene position: 3969954-3969343 (Counterclockwise)

Preceding gene: 146301071

Following gene: 146301064

Centisome position: 65.11

GC content: 34.8

Gene sequence:

>612_bases
ATGAGCCAAAAAATTCTACGCATAATTACCAGTACCAATGGTGATACTTCATTTAGTAATCAGCTGTCGAATGCTGTTAT
TGAAAAATTAACTGCAGGAAATCCTGAAACTGAAGTAAAAACTCTTGACCTTACTAAAACACCTTTACCATATTTGACTA
ATTCGCATATAAGTGCTGTATATGCTCCTGAGGAAACTCATACTCCGGAACAGAAAGAAGCATTAAAATATTCAAATGAA
GCAATAAAAACTTTATTAGAATCGGATGTAATTGTAATTGGTGTGCCTTTGTACAATTTTGGTATTCCGGCTGTTTTAAA
AGGCTGGATTGATCAGATTGCGAGAGCAGGAAAAACTTTTAGTTACAGTGCCGAAGGACCAAAAGGATTGGTAACTGATA
AAAAAGTGTATTTATCAATTGCATCTGGTGCTATTTTTTCTGAAGGACCATACAAAAGCTACGATTTTTCAGAATCGTAT
CTTCGAGCGGTATTTGGTTTTTTAGGAATGACGGATGTTACTACTTTTCGTGTAGAAGGAACTGCAATTCCTGATTTTGC
CGAAAATGCTTTACCAAAAGCCTTATCTTCTGTAGAAGAATTTGCTTTTTAA

Upstream 100 bases:

>100_bases
ATGTTACTATCCTCAATGAAAGCGCTATCCTTGAGGATAGTACTATAAAAGAGATAGCAAATGTAGATAAGTTTGTATCG
TAAAACAAATTATATAAATT

Downstream 100 bases:

>100_bases
GATAAACTATAATTACGACGGTTGAACATTCTCGTATGTAGTAAACCCGGCAGGTTTTTAAAATCTGCCGGGTTTTTAAT
ATCAAAAATAATTACTTTTC

Product: NAD(P)H dehydrogenase (quinone)

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase [H]

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MSQKILRIITSTNGDTSFSNQLSNAVIEKLTAGNPETEVKTLDLTKTPLPYLTNSHISAVYAPEETHTPEQKEALKYSNE
AIKTLLESDVIVIGVPLYNFGIPAVLKGWIDQIARAGKTFSYSAEGPKGLVTDKKVYLSIASGAIFSEGPYKSYDFSESY
LRAVFGFLGMTDVTTFRVEGTAIPDFAENALPKALSSVEEFAF

Sequences:

>Translated_203_residues
MSQKILRIITSTNGDTSFSNQLSNAVIEKLTAGNPETEVKTLDLTKTPLPYLTNSHISAVYAPEETHTPEQKEALKYSNE
AIKTLLESDVIVIGVPLYNFGIPAVLKGWIDQIARAGKTFSYSAEGPKGLVTDKKVYLSIASGAIFSEGPYKSYDFSESY
LRAVFGFLGMTDVTTFRVEGTAIPDFAENALPKALSSVEEFAF
>Mature_202_residues
SQKILRIITSTNGDTSFSNQLSNAVIEKLTAGNPETEVKTLDLTKTPLPYLTNSHISAVYAPEETHTPEQKEALKYSNEA
IKTLLESDVIVIGVPLYNFGIPAVLKGWIDQIARAGKTFSYSAEGPKGLVTDKKVYLSIASGAIFSEGPYKSYDFSESYL
RAVFGFLGMTDVTTFRVEGTAIPDFAENALPKALSSVEEFAF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=197, Percent_Identity=38.0710659898477, Blast_Score=125, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 22133; Mature: 22002

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKILRIITSTNGDTSFSNQLSNAVIEKLTAGNPETEVKTLDLTKTPLPYLTNSHISAV
CCCHHEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEE
YAPEETHTPEQKEALKYSNEAIKTLLESDVIVIGVPLYNFGIPAVLKGWIDQIARAGKTF
ECCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECHHHCCHHHHHHHHHHHHHHCCCCE
SYSAEGPKGLVTDKKVYLSIASGAIFSEGPYKSYDFSESYLRAVFGFLGMTDVTTFRVEG
EECCCCCCCCCCCCEEEEEEECCCEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEC
TAIPDFAENALPKALSSVEEFAF
CCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQKILRIITSTNGDTSFSNQLSNAVIEKLTAGNPETEVKTLDLTKTPLPYLTNSHISAV
CCHHEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEE
YAPEETHTPEQKEALKYSNEAIKTLLESDVIVIGVPLYNFGIPAVLKGWIDQIARAGKTF
ECCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECHHHCCHHHHHHHHHHHHHHCCCCE
SYSAEGPKGLVTDKKVYLSIASGAIFSEGPYKSYDFSESYLRAVFGFLGMTDVTTFRVEG
EECCCCCCCCCCCCEEEEEEECCCEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEC
TAIPDFAENALPKALSSVEEFAF
CCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA