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The map label for this gene is ilvR [H]

Identifier: 146301043

GI number: 146301043

Start: 3942518

End: 3943381

Strand: Reverse

Name: ilvR [H]

Synonym: Fjoh_3299

Alternate gene names: 146301043

Gene position: 3943381-3942518 (Counterclockwise)

Preceding gene: 146301048

Following gene: 146301037

Centisome position: 64.68

GC content: 36.57

Gene sequence:

>864_bases
ATGGATTTACAGCAGATTAAATATTTTCTGGCTCTGGCCCGCGAACTTCATTTCTGGAATACTGCCGGAAAAATGAATAT
TACACAATCGGCTTTAAGCCGTCAGATTCAGTCTCTTGAAAACCAGCTTGGTGTTCAGCTTTTTGAACGTAATAAACGAA
ATGTCAAGCTTACGTCAGCCGGCAGATTTCTTCAGGAAAAATGGGAAGTAGAACTCAGTAAACTGGAGTATATACATCAA
TCGGCGCGTCAGATTCATTTAGGCGAAAGCGGTACACTTACCATTTCTCATCCGGATTCTATTTCGGCTTCAATTATGCC
GGATATTTTATCGCGTATTTCTGATGCATTTCCCAAATTAAAAATCAAACTGGTTCAGGTTTTGTATGAAAACCAGCAGG
AATTTCTCCGCAATTATAAAATCGATCTGGCGATTACAAGAGATATTAATCACGCCAGAGATATAAAATCAGAAAAAATC
TACACTGATAATCTAGCAATTGTAGTTCCAGAAAATCATCCTTATCAAAAGCCCGAAGATCTTACGAAAGAATCATTGGC
AAATCAAAAATTTATACTGCCTACTACAGATGAAGGAAGCAGTTACAGCGATTTAATACAACGATTGTTTAACTCTTTTG
AAAATGCACCCGAAGTTTATCTTCATTCTGAATTTGGTTCGGCTATAATTGCTTTGGTTCGAAAAGGACTTGGTATAGCG
ATATTACCGGATTCGTATGTTTTTCATGAAATTTCAGGAATCAGATTTATCAAACTTCCTTTAGAAACTGATCTTTATAT
CAATTGGAGAGCCGAAGATCATAATCCGGTTTTGGCCAATGTTTTAAAACTGGTTGTTCTTTAA

Upstream 100 bases:

>100_bases
TCACTAATCAGCGCTTTGCGGACTTTTATTGTGTTCATTTTTTTTATTATTAATGCATTAAAATCTGTTTTTTGTTTTTA
TAAATGCGTAAATTGCATCA

Downstream 100 bases:

>100_bases
ACTTGTATTTAAGCTCTCAGCATTTCAATATGCGGAATTCCGTCTTCATCATATTGTTCTCCCTGCTCAACAAATCCTAA
TGACTGGTAAAATTTTGATA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Isoleucine biosynthesis transcriptional activator [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MDLQQIKYFLALARELHFWNTAGKMNITQSALSRQIQSLENQLGVQLFERNKRNVKLTSAGRFLQEKWEVELSKLEYIHQ
SARQIHLGESGTLTISHPDSISASIMPDILSRISDAFPKLKIKLVQVLYENQQEFLRNYKIDLAITRDINHARDIKSEKI
YTDNLAIVVPENHPYQKPEDLTKESLANQKFILPTTDEGSSYSDLIQRLFNSFENAPEVYLHSEFGSAIIALVRKGLGIA
ILPDSYVFHEISGIRFIKLPLETDLYINWRAEDHNPVLANVLKLVVL

Sequences:

>Translated_287_residues
MDLQQIKYFLALARELHFWNTAGKMNITQSALSRQIQSLENQLGVQLFERNKRNVKLTSAGRFLQEKWEVELSKLEYIHQ
SARQIHLGESGTLTISHPDSISASIMPDILSRISDAFPKLKIKLVQVLYENQQEFLRNYKIDLAITRDINHARDIKSEKI
YTDNLAIVVPENHPYQKPEDLTKESLANQKFILPTTDEGSSYSDLIQRLFNSFENAPEVYLHSEFGSAIIALVRKGLGIA
ILPDSYVFHEISGIRFIKLPLETDLYINWRAEDHNPVLANVLKLVVL
>Mature_287_residues
MDLQQIKYFLALARELHFWNTAGKMNITQSALSRQIQSLENQLGVQLFERNKRNVKLTSAGRFLQEKWEVELSKLEYIHQ
SARQIHLGESGTLTISHPDSISASIMPDILSRISDAFPKLKIKLVQVLYENQQEFLRNYKIDLAITRDINHARDIKSEKI
YTDNLAIVVPENHPYQKPEDLTKESLANQKFILPTTDEGSSYSDLIQRLFNSFENAPEVYLHSEFGSAIIALVRKGLGIA
ILPDSYVFHEISGIRFIKLPLETDLYINWRAEDHNPVLANVLKLVVL

Specific function: Positively regulates the expression of the ilvD gene while negatively autoregulating its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=276, Percent_Identity=29.3478260869565, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI2367136, Length=284, Percent_Identity=27.8169014084507, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788887, Length=294, Percent_Identity=27.5510204081633, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1790399, Length=245, Percent_Identity=26.530612244898, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787601, Length=290, Percent_Identity=26.8965517241379, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI157672245, Length=263, Percent_Identity=25.8555133079848, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788481, Length=287, Percent_Identity=24.7386759581882, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=30.6772908366534, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI145693105, Length=249, Percent_Identity=26.1044176706827, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788297, Length=258, Percent_Identity=24.8062015503876, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787530, Length=245, Percent_Identity=24.8979591836735, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788748, Length=247, Percent_Identity=25.1012145748988, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789204, Length=271, Percent_Identity=23.6162361623616, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788296, Length=245, Percent_Identity=25.3061224489796, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789173, Length=192, Percent_Identity=29.6875, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787806, Length=249, Percent_Identity=26.5060240963855, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994962, Length=149, Percent_Identity=33.5570469798658, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789506, Length=215, Percent_Identity=24.1860465116279, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789492, Length=279, Percent_Identity=23.6559139784946, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32958; Mature: 32958

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLQQIKYFLALARELHFWNTAGKMNITQSALSRQIQSLENQLGVQLFERNKRNVKLTSA
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHH
GRFLQEKWEVELSKLEYIHQSARQIHLGESGTLTISHPDSISASIMPDILSRISDAFPKL
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
KIKLVQVLYENQQEFLRNYKIDLAITRDINHARDIKSEKIYTDNLAIVVPENHPYQKPED
HHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHCCCCCCEEECCEEEEECCCCCCCCCHH
LTKESLANQKFILPTTDEGSSYSDLIQRLFNSFENAPEVYLHSEFGSAIIALVRKGLGIA
HHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHCCCCEE
ILPDSYVFHEISGIRFIKLPLETDLYINWRAEDHNPVLANVLKLVVL
ECCCCHHHHHHCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MDLQQIKYFLALARELHFWNTAGKMNITQSALSRQIQSLENQLGVQLFERNKRNVKLTSA
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHH
GRFLQEKWEVELSKLEYIHQSARQIHLGESGTLTISHPDSISASIMPDILSRISDAFPKL
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
KIKLVQVLYENQQEFLRNYKIDLAITRDINHARDIKSEKIYTDNLAIVVPENHPYQKPED
HHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHCCCCCCEEECCEEEEECCCCCCCCCHH
LTKESLANQKFILPTTDEGSSYSDLIQRLFNSFENAPEVYLHSEFGSAIIALVRKGLGIA
HHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHCCCCEE
ILPDSYVFHEISGIRFIKLPLETDLYINWRAEDHNPVLANVLKLVVL
ECCCCHHHHHHCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8113165; 11259647 [H]