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The map label for this gene is bcr [H]

Identifier: 146300602

GI number: 146300602

Start: 3383856

End: 3385085

Strand: Reverse

Name: bcr [H]

Synonym: Fjoh_2852

Alternate gene names: 146300602

Gene position: 3385085-3383856 (Counterclockwise)

Preceding gene: 146300604

Following gene: 146300597

Centisome position: 55.52

GC content: 34.31

Gene sequence:

>1230_bases
ATGACAACAAAAAAATACCTCAAACTCATTCTCATTTTAGGTTCTTTAACCGCTCTTGGTCCTTTTTCAATCGATATGTA
CTTACCTGGATTCTCTGGTATTGCAAAAGATTTGAATACTTCGGTAGCCAAAGTTTCGATGAGTTTATCTAGTTATTTCA
TTGGAATTTCAGCTGGACAGCTGCTTTACGGCCCATTATTAGACCGTTTTGGACGCAAAAAACCATTGTTTCTTGGTTTA
ATGGTTTATATTTTAGCTTCTTTGGGCTGTTCGTATGTAACTGATATCGATACATTTATTTTCTTGCGTTTTATTCAGGC
TATCGGAAGCTGTGCCGCAACAGTTGCTTCTGTAGCAATGGTTCGTGATTTATTTCCTGTAAAAGATATTCCAAAAGTAT
TTTCATTGTTAATGCTTGTTTTAGGGCTTTCGCCGATGCTGGCGCCTACAATTGGCGGTTATGTTACCGAAGATTATGGC
TGGCATACTGTATTTTCTATACTTATGTTTATGGGAATTGCTATTTTGATTGCTGCTCAAATTGGTTTGCCAAATAGTTA
CAAACCAGACACTTCAATATCATTAAAACCAAAACCAATTATTTCGAACTTTTTAAAAGTTCTTAAAGAACCACAATTTT
ATACATATGCATTTACCGGAGCAATCGCATTTTCGGGATTATTTACGTATGTAGCGGCCTCTCCTATCATTTTTATGGAT
ATTTATCATGTTGATGCTAAAACTTATGGATGGATTTTTGCCTTTATGTCTGTAAGTTTTATTGGTTCAAGTCAGTTAAA
TTCTGTTTTATTAAAGAGATTTTCAAGTGAACAGATGATTTTTGGAGCATTAATCCTGCAGTCTGTCGTTAGTATTATTT
TCCTAATACTTTCTTTAAACAATTTATTGGGCTTATATGAAACTATAGCAATCCTGTTTATTTTTCTGGGTTGTTTAGGA
ATTTCAAACCCTAATACTGCTGGGCTTACAATGGCTCCTTTTGCAAAAAATGCAGGAAGTGCTTCGGCTTTAATGGGCGC
AATTCAGTTAGGATTAGGATCTTTGGCCTCATTTGCTGTTGGAATTTTTGTAAAGGATTCTGTAGCGCCTATGGTTCTTA
TTATGACTGTAACTACAATTATTGCCTTTATAGTTTTAAATATCGGAAAAAGATTTATCAAAAATAAAGTAGAAATAGCT
GCTGATGATGACACTATACTTGCTCATTAA

Upstream 100 bases:

>100_bases
AGCTTGTATAATACCCAGTTTTGATAAAGGATAATTAATATACCTAACAAGATACTAAATATTTATCTTTGCAGGAAATT
ATAAGCCTCTTTTTAATAAA

Downstream 100 bases:

>100_bases
TGAGTAAAAATTTCAATAAAAAAGCCAGAATCAAAAATTCTGGCTTTTTTTATAGTATCTCAATCTATTGGAATTTCTTA
TTTCTCCTGAGATCTTTTAT

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTTKKYLKLILILGSLTALGPFSIDMYLPGFSGIAKDLNTSVAKVSMSLSSYFIGISAGQLLYGPLLDRFGRKKPLFLGL
MVYILASLGCSYVTDIDTFIFLRFIQAIGSCAATVASVAMVRDLFPVKDIPKVFSLLMLVLGLSPMLAPTIGGYVTEDYG
WHTVFSILMFMGIAILIAAQIGLPNSYKPDTSISLKPKPIISNFLKVLKEPQFYTYAFTGAIAFSGLFTYVAASPIIFMD
IYHVDAKTYGWIFAFMSVSFIGSSQLNSVLLKRFSSEQMIFGALILQSVVSIIFLILSLNNLLGLYETIAILFIFLGCLG
ISNPNTAGLTMAPFAKNAGSASALMGAIQLGLGSLASFAVGIFVKDSVAPMVLIMTVTTIIAFIVLNIGKRFIKNKVEIA
ADDDTILAH

Sequences:

>Translated_409_residues
MTTKKYLKLILILGSLTALGPFSIDMYLPGFSGIAKDLNTSVAKVSMSLSSYFIGISAGQLLYGPLLDRFGRKKPLFLGL
MVYILASLGCSYVTDIDTFIFLRFIQAIGSCAATVASVAMVRDLFPVKDIPKVFSLLMLVLGLSPMLAPTIGGYVTEDYG
WHTVFSILMFMGIAILIAAQIGLPNSYKPDTSISLKPKPIISNFLKVLKEPQFYTYAFTGAIAFSGLFTYVAASPIIFMD
IYHVDAKTYGWIFAFMSVSFIGSSQLNSVLLKRFSSEQMIFGALILQSVVSIIFLILSLNNLLGLYETIAILFIFLGCLG
ISNPNTAGLTMAPFAKNAGSASALMGAIQLGLGSLASFAVGIFVKDSVAPMVLIMTVTTIIAFIVLNIGKRFIKNKVEIA
ADDDTILAH
>Mature_408_residues
TTKKYLKLILILGSLTALGPFSIDMYLPGFSGIAKDLNTSVAKVSMSLSSYFIGISAGQLLYGPLLDRFGRKKPLFLGLM
VYILASLGCSYVTDIDTFIFLRFIQAIGSCAATVASVAMVRDLFPVKDIPKVFSLLMLVLGLSPMLAPTIGGYVTEDYGW
HTVFSILMFMGIAILIAAQIGLPNSYKPDTSISLKPKPIISNFLKVLKEPQFYTYAFTGAIAFSGLFTYVAASPIIFMDI
YHVDAKTYGWIFAFMSVSFIGSSQLNSVLLKRFSSEQMIFGALILQSVVSIIFLILSLNNLLGLYETIAILFIFLGCLGI
SNPNTAGLTMAPFAKNAGSASALMGAIQLGLGSLASFAVGIFVKDSVAPMVLIMTVTTIIAFIVLNIGKRFIKNKVEIAA
DDDTILAH

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=374, Percent_Identity=28.8770053475936, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI48994889, Length=392, Percent_Identity=28.5714285714286, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1790146, Length=383, Percent_Identity=28.1984334203655, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790794, Length=271, Percent_Identity=23.6162361623616, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI87082312, Length=353, Percent_Identity=24.0793201133144, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787065, Length=331, Percent_Identity=24.1691842900302, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790195, Length=153, Percent_Identity=29.4117647058824, Blast_Score=66, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319480, Length=265, Percent_Identity=21.8867924528302, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44148; Mature: 44017

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEECC
>Mature Secondary Structure 
TTKKYLKLILILGSLTALGPFSIDMYLPGFSGIAKDLNTSVAKVSMSLSSYFIGISAGQ
CHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCH
LLYGPLLDRFGRKKPLFLGLMVYILASLGCSYVTDIDTFIFLRFIQAIGSCAATVASVAM
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRDLFPVKDIPKVFSLLMLVLGLSPMLAPTIGGYVTEDYGWHTVFSILMFMGIAILIAAQ
HHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IGLPNSYKPDTSISLKPKPIISNFLKVLKEPQFYTYAFTGAIAFSGLFTYVAASPIIFMD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCEEEEE
IYHVDAKTYGWIFAFMSVSFIGSSQLNSVLLKRFSSEQMIFGALILQSVVSIIFLILSLN
EEEECCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
NLLGLYETIAILFIFLGCLGISNPNTAGLTMAPFAKNAGSASALMGAIQLGLGSLASFAV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GIFVKDSVAPMVLIMTVTTIIAFIVLNIGKRFIKNKVEIAADDDTILAH
HHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]