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The map label for this gene is tetX [H]

Identifier: 146300573

GI number: 146300573

Start: 3358458

End: 3359573

Strand: Reverse

Name: tetX [H]

Synonym: Fjoh_2823

Alternate gene names: 146300573

Gene position: 3359573-3358458 (Counterclockwise)

Preceding gene: 146300574

Following gene: 146300572

Centisome position: 55.1

GC content: 35.84

Gene sequence:

>1116_bases
ATGCTACTTACAAATAAAAAAACAGCCATTATAGGCGCCGGGCCGGTTGGTTTAACAATGGCCAGATTATTACAGCAAAA
CGGAATTAATGTTACCGTTTACGAAAGAGACAAAGACCCAAAAGCAAGAATCTGGGGCGGTACGCTGGATTTACACAAAG
GTTCTGGGCAAGATGCGATGCAAAAGGCGGGACTGCTTGAGGAATACTACAAAAAGGCAATTCCGATGGGGAGAATCATA
ACCGATTATCTTGGAAATGTATTGTTTAGCAAAGAAACATCGGTCGAAGAACAATATGACGGCCCGGAAATCAACCGAAA
CGACTTAAGAACATTACTAATCAACAGCTTAGAAAAAGATACTGTGATTTGGGATAGAAAACTGATTAAGGCCGAAGAAC
AAAACAATAAATGGGTTTTACATTTTGAAAATGGCGAAATTGAAACCGCAGATTTTGTTATTGGAGCAAATGGCGGCATG
ACTGCTATTAGAAAATATCTTACTGATGCAGCAGTAGAATTTACAGGAACTTATATGATACAAGGCGAAGTATCTGAACC
AGAGATAAAATGCCCTGATTTTTACAAACTATGTGCTGATAAAATTTTAATGACTTCTAATAATGGGAATTTATTAGTTG
CAAACCCTAAGAACAGTAATGTTTTGAGTTATAATGTGATTTTTAAAATGCCAGAAATACTTGAATTGGACTTTAAAAAT
TCTGACTCTGTAATTGAGTTTATTTCAGAAAGATTTAAGGATTGGGACGCTGTTTACAAACAATTATTTCAAGCTGCTTC
ATTTTTTACTGGTTTGCCCACAAAAAAAATATCAGTCGAGGAATGGAAAAATAATCGTCCCTTACCTATTACGCTTATTG
GCGATGCAGCGCATTTAATGCCTCCTTTTGCGGGACAAGGAGCTAATATCGGGCTTCTGGATGCTTTAATTCTATCAGAA
AACTTAACAAACAGAGATTTTAAAACCATAGAAGAAGCAGTTTTTGATTATGAACAGCAAATGCAGCTTTATGCACGCAA
AGCACAAGAAGAAACCCGAATAAATGAAATTAAAATGCTGAAAAGGGATTTTACATTTTTAAAATTTGCCAATTAA

Upstream 100 bases:

>100_bases
ACAGCGATTAAAAAGTTCAACAAATAAAAACGTCTGATTTTTACTATTTCATCCACTCCTTATTTTTCAATTTTGTAGCA
TAATTCAAAGAATAAAACTT

Downstream 100 bases:

>100_bases
ATATTTGGTATTTGTGATGAAAATAATTCTATCTTAGTAACAGAAAACGAACCTGATAATTTGATCTAAAATATGGAAAA
CTCACAAAATCAATCTGATG

Product: FAD-binding monooxygenase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MLLTNKKTAIIGAGPVGLTMARLLQQNGINVTVYERDKDPKARIWGGTLDLHKGSGQDAMQKAGLLEEYYKKAIPMGRII
TDYLGNVLFSKETSVEEQYDGPEINRNDLRTLLINSLEKDTVIWDRKLIKAEEQNNKWVLHFENGEIETADFVIGANGGM
TAIRKYLTDAAVEFTGTYMIQGEVSEPEIKCPDFYKLCADKILMTSNNGNLLVANPKNSNVLSYNVIFKMPEILELDFKN
SDSVIEFISERFKDWDAVYKQLFQAASFFTGLPTKKISVEEWKNNRPLPITLIGDAAHLMPPFAGQGANIGLLDALILSE
NLTNRDFKTIEEAVFDYEQQMQLYARKAQEETRINEIKMLKRDFTFLKFAN

Sequences:

>Translated_371_residues
MLLTNKKTAIIGAGPVGLTMARLLQQNGINVTVYERDKDPKARIWGGTLDLHKGSGQDAMQKAGLLEEYYKKAIPMGRII
TDYLGNVLFSKETSVEEQYDGPEINRNDLRTLLINSLEKDTVIWDRKLIKAEEQNNKWVLHFENGEIETADFVIGANGGM
TAIRKYLTDAAVEFTGTYMIQGEVSEPEIKCPDFYKLCADKILMTSNNGNLLVANPKNSNVLSYNVIFKMPEILELDFKN
SDSVIEFISERFKDWDAVYKQLFQAASFFTGLPTKKISVEEWKNNRPLPITLIGDAAHLMPPFAGQGANIGLLDALILSE
NLTNRDFKTIEEAVFDYEQQMQLYARKAQEETRINEIKMLKRDFTFLKFAN
>Mature_371_residues
MLLTNKKTAIIGAGPVGLTMARLLQQNGINVTVYERDKDPKARIWGGTLDLHKGSGQDAMQKAGLLEEYYKKAIPMGRII
TDYLGNVLFSKETSVEEQYDGPEINRNDLRTLLINSLEKDTVIWDRKLIKAEEQNNKWVLHFENGEIETADFVIGANGGM
TAIRKYLTDAAVEFTGTYMIQGEVSEPEIKCPDFYKLCADKILMTSNNGNLLVANPKNSNVLSYNVIFKMPEILELDFKN
SDSVIEFISERFKDWDAVYKQLFQAASFFTGLPTKKISVEEWKNNRPLPITLIGDAAHLMPPFAGQGANIGLLDALILSE
NLTNRDFKTIEEAVFDYEQQMQLYARKAQEETRINEIKMLKRDFTFLKFAN

Specific function: Resistance to tetracycline via an oxidoreductase activity (Potential) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI187960045, Length=330, Percent_Identity=24.2424242424242, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17562934, Length=385, Percent_Identity=25.7142857142857, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28573964, Length=334, Percent_Identity=26.0479041916168, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR016040
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 42077; Mature: 42077

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLTNKKTAIIGAGPVGLTMARLLQQNGINVTVYERDKDPKARIWGGTLDLHKGSGQDAM
CCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCEEEEECCCCHHHH
QKAGLLEEYYKKAIPMGRIITDYLGNVLFSKETSVEEQYDGPEINRNDLRTLLINSLEKD
HHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCCCHHHCCCCCCCHHHHHHHHHHHCCCC
TVIWDRKLIKAEEQNNKWVLHFENGEIETADFVIGANGGMTAIRKYLTDAAVEFTGTYMI
HHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEEECCCCHHHHHHHHHHHHHEEEEEEEE
QGEVSEPEIKCPDFYKLCADKILMTSNNGNLLVANPKNSNVLSYNVIFKMPEILELDFKN
EECCCCCCCCCCHHHHHHHCCEEEECCCCCEEEECCCCCCEEEEEEEECCCHHEEECCCC
SDSVIEFISERFKDWDAVYKQLFQAASFFTGLPTKKISVEEWKNNRPLPITLIGDAAHLM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCCCEEEEEECCHHHHC
PPFAGQGANIGLLDALILSENLTNRDFKTIEEAVFDYEQQMQLYARKAQEETRINEIKML
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRDFTFLKFAN
HHCCCEEEECC
>Mature Secondary Structure
MLLTNKKTAIIGAGPVGLTMARLLQQNGINVTVYERDKDPKARIWGGTLDLHKGSGQDAM
CCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCEEEEECCCCHHHH
QKAGLLEEYYKKAIPMGRIITDYLGNVLFSKETSVEEQYDGPEINRNDLRTLLINSLEKD
HHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCCCHHHCCCCCCCHHHHHHHHHHHCCCC
TVIWDRKLIKAEEQNNKWVLHFENGEIETADFVIGANGGMTAIRKYLTDAAVEFTGTYMI
HHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEEECCCCHHHHHHHHHHHHHEEEEEEEE
QGEVSEPEIKCPDFYKLCADKILMTSNNGNLLVANPKNSNVLSYNVIFKMPEILELDFKN
EECCCCCCCCCCHHHHHHHCCEEEECCCCCEEEECCCCCCEEEEEEEECCCHHEEECCCC
SDSVIEFISERFKDWDAVYKQLFQAASFFTGLPTKKISVEEWKNNRPLPITLIGDAAHLM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCCCEEEEEECCHHHHC
PPFAGQGANIGLLDALILSENLTNRDFKTIEEAVFDYEQQMQLYARKAQEETRINEIKML
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRDFTFLKFAN
HHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1846135 [H]