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The map label for this gene is speE [C]

Identifier: 146300551

GI number: 146300551

Start: 3333965

End: 3334630

Strand: Reverse

Name: speE [C]

Synonym: Fjoh_2801

Alternate gene names: 146300551

Gene position: 3334630-3333965 (Counterclockwise)

Preceding gene: 146300553

Following gene: 146300550

Centisome position: 54.69

GC content: 31.53

Gene sequence:

>666_bases
ATGATTCAAAAACTATTCAGTTATCTTTATCCAATTAAAATCTTCAAAAAAAAATCGGCCAGAAGCAAAGTTATCGAAGT
TACCTGGGCAAATGGAGAGCTGGTTTTAGATTCTGAAAACACTAATTACTCTTACGGAAGTCTGCAGCGTATATTAAGGT
ACGGACTTCGAAATATTGGTTACAATAAAGTTCTCGAAATGGATCATATATTGGTTCTGGGAGTTGCCGGTGGCAGCGTA
ATCAAAACGCTGGTTGATGAAATCGAATATAAAGGAAAAATTACGGGTGTTGAAATTGATGCCGAGATGATTCAGATTGC
CAATCAGTATTTTAACTTAAATGAAATACAACAGCTCGAAATTGTAATTGATGATGCTTTTGAATTTGTTTTAAAAACAA
AAGATAAATATGATTTAATAATTATTGATATTTTTGAAGATACCAATATGCCGAACTTTTTGTTTGAAAAGTTTTTCAGC
GAAAGAATTTGTTTTCTTTTAAAAGATCGGGGCTTTGTTTTATTTAATACTATGATTTTAGACGAAGCTCATAATGTTCG
AAACAGAAAGTATATATCTGAAATAAATCCAGAATTGTTTACTTCAAAAATGCTGCCCCGCATAGAAGTGCATAATGAAC
TAATAATAATCGAAAAGCTGCCTTAA

Upstream 100 bases:

>100_bases
TTATAAAAGATGAATTTGTATAGTCTTTTCTTCATTAAAATTGATATTTCTTGCAAAATAAAAGATTTAAAATGGTATTT
TTGAGTAAAAGCATTACAAA

Downstream 100 bases:

>100_bases
ATATTATCTTTATGAAACCTCTTGAATCTATATTAAAAGCCTTACCACCTACTAAAATTATTGATGCCTCTATTGATTTT
TCAAAATATCTTCCTTTAGA

Product: spermine synthase

Products: 5-Methylthioadenosine; H+; Spermidine [C]

Alternate protein names: Spermidine Synthase

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MIQKLFSYLYPIKIFKKKSARSKVIEVTWANGELVLDSENTNYSYGSLQRILRYGLRNIGYNKVLEMDHILVLGVAGGSV
IKTLVDEIEYKGKITGVEIDAEMIQIANQYFNLNEIQQLEIVIDDAFEFVLKTKDKYDLIIIDIFEDTNMPNFLFEKFFS
ERICFLLKDRGFVLFNTMILDEAHNVRNRKYISEINPELFTSKMLPRIEVHNELIIIEKLP

Sequences:

>Translated_221_residues
MIQKLFSYLYPIKIFKKKSARSKVIEVTWANGELVLDSENTNYSYGSLQRILRYGLRNIGYNKVLEMDHILVLGVAGGSV
IKTLVDEIEYKGKITGVEIDAEMIQIANQYFNLNEIQQLEIVIDDAFEFVLKTKDKYDLIIIDIFEDTNMPNFLFEKFFS
ERICFLLKDRGFVLFNTMILDEAHNVRNRKYISEINPELFTSKMLPRIEVHNELIIIEKLP
>Mature_221_residues
MIQKLFSYLYPIKIFKKKSARSKVIEVTWANGELVLDSENTNYSYGSLQRILRYGLRNIGYNKVLEMDHILVLGVAGGSV
IKTLVDEIEYKGKITGVEIDAEMIQIANQYFNLNEIQQLEIVIDDAFEFVLKTKDKYDLIIIDIFEDTNMPNFLFEKFFS
ERICFLLKDRGFVLFNTMILDEAHNVRNRKYISEINPELFTSKMLPRIEVHNELIIIEKLP

Specific function: Spermidine biosynthesis from arginine; third (last) step. [C]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.5.1.16 [C]

Molecular weight: Translated: 25861; Mature: 25861

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQKLFSYLYPIKIFKKKSARSKVIEVTWANGELVLDSENTNYSYGSLQRILRYGLRNIG
CHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHCC
YNKVLEMDHILVLGVAGGSVIKTLVDEIEYKGKITGVEIDAEMIQIANQYFNLNEIQQLE
CHHHEEECCEEEEEECCCHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHCCCCHHHEEE
IVIDDAFEFVLKTKDKYDLIIIDIFEDTNMPNFLFEKFFSERICFLLKDRGFVLFNTMIL
EEHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHEEEEECCCEEEEEEEHH
DEAHNVRNRKYISEINPELFTSKMLPRIEVHNELIIIEKLP
HHHHHHHHHHHHHHCCHHHHHHHCCCCEEECCCEEEEEECC
>Mature Secondary Structure
MIQKLFSYLYPIKIFKKKSARSKVIEVTWANGELVLDSENTNYSYGSLQRILRYGLRNIG
CHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHCC
YNKVLEMDHILVLGVAGGSVIKTLVDEIEYKGKITGVEIDAEMIQIANQYFNLNEIQQLE
CHHHEEECCEEEEEECCCHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHCCCCHHHEEE
IVIDDAFEFVLKTKDKYDLIIIDIFEDTNMPNFLFEKFFSERICFLLKDRGFVLFNTMIL
EEHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHEEEEECCCEEEEEEEHH
DEAHNVRNRKYISEINPELFTSKMLPRIEVHNELIIIEKLP
HHHHHHHHHHHHHHCCHHHHHHHCCCCEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0022 {S-adenosyl-3-methylthio-1-propylamine}} 0.09 {1,4-diaminobutane}} 0.012 {1,4-diaminobutane}} [C]

Substrates: S-Adenosylmethioninamine; Putrescine [C]

Specific reaction: S-Adenosylmethioninamine + Putrescine --> 5-Methylthioadenosine + H+ + Spermidine [C]

General reaction: Aminopropyl group transfer [C]

Inhibitor: 5'-Ethyl thioadenosine; 5'-Methyl thioadenosine; Dicyclohexyl amine; carbonyl binding reagents, [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA