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The map label for this gene is nth [H]

Identifier: 146300490

GI number: 146300490

Start: 3270366

End: 3271016

Strand: Reverse

Name: nth [H]

Synonym: Fjoh_2740

Alternate gene names: 146300490

Gene position: 3271016-3270366 (Counterclockwise)

Preceding gene: 146300491

Following gene: 146300486

Centisome position: 53.65

GC content: 31.95

Gene sequence:

>651_bases
ATGGATTTGTTTGGCGAAACTGAAAATTGGGAAACTAAATTAAAACCCATTTTAAAAAAATACAAGCACAAAAAGCATCC
TTTAGATTATCAAAACACATATCAGCTTTTGGTAATGGTAGTTTTATCTGCACAGGATTCTGATGCTAACATCAACAAGA
TTGCACCTGCATTATTTGAAAAATATCCAACTTTAAAAAGTCTGTCAAAAGCTGATATTGATACTTTTATTTCTTACATA
AGTAAAGTACGTAATTATCCAACTAAGGCTCAATGGCTTTTAGAAATTGCACACACCATTCAAAATGATAATGATATTCC
TTTAACAATGTCTGGTTTGACTGCCTTGAAAGGAATTGGAAGAAAATCTGCCAATGTAATTTTAAGAGAAACTGAACAGC
CGGCTGAAGGAATTATTGCCGATTTACATGTAATTCGCGTAGCACCTAGAATTGGAATAATAAAGGAATCTAAAGATGGT
AATAAAGTCGAAAAAGACTTAATGCAGGCTTTACCAAAAAGTATTTGGTCAGAAATTGGAATGGCAATTTCATTTTTAGG
TCGTGAAATCTGCAGACCAAAACCAAAGTGTGAAGAATGTCTTTTAACAGAAATCTGTTTGTATTATAATACTGTCGTAC
TAAAAAATTAG

Upstream 100 bases:

>100_bases
ACTTGGAAATCAAATTGTTATAAAAAAGATATTAGAATTCATTAAAATCAATTAACTTTATAGATAACTATTTCAATATC
AACATTAAAGAAAGTATAAG

Downstream 100 bases:

>100_bases
ATTATTTTCTACGTGTATCAATCAGGTAAATAATTTTCCAGGAATCTTTTTCTTTAAACAATGTAAAAGTGTTTACTCCT
GCATGACTTAATTTATCATT

Product: DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MDLFGETENWETKLKPILKKYKHKKHPLDYQNTYQLLVMVVLSAQDSDANINKIAPALFEKYPTLKSLSKADIDTFISYI
SKVRNYPTKAQWLLEIAHTIQNDNDIPLTMSGLTALKGIGRKSANVILRETEQPAEGIIADLHVIRVAPRIGIIKESKDG
NKVEKDLMQALPKSIWSEIGMAISFLGREICRPKPKCEECLLTEICLYYNTVVLKN

Sequences:

>Translated_216_residues
MDLFGETENWETKLKPILKKYKHKKHPLDYQNTYQLLVMVVLSAQDSDANINKIAPALFEKYPTLKSLSKADIDTFISYI
SKVRNYPTKAQWLLEIAHTIQNDNDIPLTMSGLTALKGIGRKSANVILRETEQPAEGIIADLHVIRVAPRIGIIKESKDG
NKVEKDLMQALPKSIWSEIGMAISFLGREICRPKPKCEECLLTEICLYYNTVVLKN
>Mature_216_residues
MDLFGETENWETKLKPILKKYKHKKHPLDYQNTYQLLVMVVLSAQDSDANINKIAPALFEKYPTLKSLSKADIDTFISYI
SKVRNYPTKAQWLLEIAHTIQNDNDIPLTMSGLTALKGIGRKSANVILRETEQPAEGIIADLHVIRVAPRIGIIKESKDG
NKVEKDLMQALPKSIWSEIGMAISFLGREICRPKPKCEECLLTEICLYYNTVVLKN

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=219, Percent_Identity=26.4840182648402, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1787920, Length=206, Percent_Identity=27.1844660194175, Blast_Score=87, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=32.2033898305085, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324530, Length=186, Percent_Identity=28.494623655914, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=29.9435028248588, Blast_Score=77, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24516; Mature: 24516

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLFGETENWETKLKPILKKYKHKKHPLDYQNTYQLLVMVVLSAQDSDANINKIAPALFE
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
KYPTLKSLSKADIDTFISYISKVRNYPTKAQWLLEIAHTIQNDNDIPLTMSGLTALKGIG
HCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCC
RKSANVILRETEQPAEGIIADLHVIRVAPRIGIIKESKDGNKVEKDLMQALPKSIWSEIG
CCHHHEEEECCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHH
MAISFLGREICRPKPKCEECLLTEICLYYNTVVLKN
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLFGETENWETKLKPILKKYKHKKHPLDYQNTYQLLVMVVLSAQDSDANINKIAPALFE
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
KYPTLKSLSKADIDTFISYISKVRNYPTKAQWLLEIAHTIQNDNDIPLTMSGLTALKGIG
HCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCC
RKSANVILRETEQPAEGIIADLHVIRVAPRIGIIKESKDGNKVEKDLMQALPKSIWSEIG
CCHHHEEEECCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHH
MAISFLGREICRPKPKCEECLLTEICLYYNTVVLKN
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]