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The map label for this gene is pyrG

Identifier: 146300138

GI number: 146300138

Start: 2855557

End: 2857170

Strand: Direct

Name: pyrG

Synonym: Fjoh_2383

Alternate gene names: 146300138

Gene position: 2855557-2857170 (Clockwise)

Preceding gene: 146300137

Following gene: 146300139

Centisome position: 46.84

GC content: 35.19

Gene sequence:

>1614_bases
ATGAATCAAACAAAATATATTTTTGTTACAGGCGGTGTGACTTCTTCATTAGGAAAAGGGATCATTGCAGCATCTTTAGC
AAAATTGTTACAAGGAAGAGGATACCGTACAACTATTCAAAAATTTGATCCGTATATTAATGTGGATCCGGGAACACTAA
ACCCGTATGAGCACGGAGAATGTTATGTAACAGATGATGGTGCAGAAACAGATTTAGATTTAGGTCATTACGAGCGTTTT
CTTAACGTTCCTACTTCTCAGGCCAATAACGTTACTACAGGAAGAGTGTATCTTTCGGTTATTGAAAAAGAAAGAAGAGG
AGAGTTTTTAGGAAAAACAGTTCAGGTTGTTCCTCACATCACAAACGAAATCAAAGACAGAATGCAGTTGCTGGGTAAAT
CTGGCGATTATGATATTGTAATTACTGAAATTGGTGGAACTGTAGGTGATATTGAATCATTACCTTATATAGAATCTGTT
CGTCAGTTAGTTTGGGAATTAGGAGAAAATAATGGAATTGTTATTCATTTAACTTTAGTTCCTTATTTGGCTGCAGCTGG
TGAGTTGAAAACAAAGCCAACACAGCACTCTGTAAAAACTTTAATGGAAAGCGGAATTAAAGCTGATATTTTGGTTTGTA
GAACTGAGCACGAATTATCTCAGGAATTACGTCAAAAACTAGCTTTGTTTTGTAATGTTAAAAAAGAAGCAGTTATCCAG
TCTATTGATGCTTCTACTATATATGAAGTTCCAAATTTAATGCTTGAAGAAGGATTAGATGTTGTAGCTTTAAAGAAATT
AGATCTGCCTAAAAAAGCATCTCCGGATCTTAAAAACTGGAATACTTTCTTAAAAAGATTAAAAAGTCCAAAACAAACTG
TAAATATTGGTTTGGTTGGAAAATATGTAGAAATGCAGGATTGTTACAAATCTATTTTAGAGGCGTTCATTCATGCAGGT
GCTGCAAATGAAACTAAAGTAAATGTAATTTCAATTCACTCAGAGCATATTAATGCTGATAACGTAGAAGAGAAATTAGG
AACTCTTGACGGAGTTTTAGTTGCTCCAGGATTTGGAGAAAGAGGTATTGAAGGAAAAATCGAAGCAGTTCGTTATGTTC
GTGAAAACAATATTCCGTTTTTCGGAATTTGTTTAGGAATGCAGATGTCTGTTATCGAGTATTCTAGAAATATTTTAGGT
TATGCCGATGCCAATTCAACAGAGATGAACGAGAAAACGCCTCATCCGGTTGTGAATTTAATGGAAGAGCAAAAAAATAT
TACAGATAAAGGCGGAACAATGCGTTTAGGAGCTTGGAAATGTGATATTAAACCAAATACACTGGCACACAGAATTTACG
GTGAAAAAACAATTTCAGAACGTCACCGCCACCGTTATGAGTACAATAATAAATATGCTGATGAATTACAGAAAGCAGGT
TTAAAAGCATCTGGAGTAAACCCGGATACCGGATTAGTTGAAATCGTAGAGCTTGAAAACCACCCGTTTTTTATTGGTGT
ACAATACCACCCAGAATACAAAAGTACAGTTGCAAATCCGCACCCAATATTTGTAAACTTTGTGGCAGCTGCTGTAAATG
CGCATAAAAAATAA

Upstream 100 bases:

>100_bases
AAAAATTTGCAAATTATCTCATTATCGAATTGATAAATTGTCTAATTAAATTTGTACTTTTGCCAAGTTTTTTACACAAC
TACTTACAAACAACAACAGA

Downstream 100 bases:

>100_bases
TAATTAATATTCTGAGAAGAATGTAACTTTTGAGATCAACTCATTACTAATAAGCTTCAACGAATAACTTTTTTAAATTA
ATAATGGAAGAAAAAAAATT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MNQTKYIFVTGGVTSSLGKGIIAASLAKLLQGRGYRTTIQKFDPYINVDPGTLNPYEHGECYVTDDGAETDLDLGHYERF
LNVPTSQANNVTTGRVYLSVIEKERRGEFLGKTVQVVPHITNEIKDRMQLLGKSGDYDIVITEIGGTVGDIESLPYIESV
RQLVWELGENNGIVIHLTLVPYLAAAGELKTKPTQHSVKTLMESGIKADILVCRTEHELSQELRQKLALFCNVKKEAVIQ
SIDASTIYEVPNLMLEEGLDVVALKKLDLPKKASPDLKNWNTFLKRLKSPKQTVNIGLVGKYVEMQDCYKSILEAFIHAG
AANETKVNVISIHSEHINADNVEEKLGTLDGVLVAPGFGERGIEGKIEAVRYVRENNIPFFGICLGMQMSVIEYSRNILG
YADANSTEMNEKTPHPVVNLMEEQKNITDKGGTMRLGAWKCDIKPNTLAHRIYGEKTISERHRHRYEYNNKYADELQKAG
LKASGVNPDTGLVEIVELENHPFFIGVQYHPEYKSTVANPHPIFVNFVAAAVNAHKK

Sequences:

>Translated_537_residues
MNQTKYIFVTGGVTSSLGKGIIAASLAKLLQGRGYRTTIQKFDPYINVDPGTLNPYEHGECYVTDDGAETDLDLGHYERF
LNVPTSQANNVTTGRVYLSVIEKERRGEFLGKTVQVVPHITNEIKDRMQLLGKSGDYDIVITEIGGTVGDIESLPYIESV
RQLVWELGENNGIVIHLTLVPYLAAAGELKTKPTQHSVKTLMESGIKADILVCRTEHELSQELRQKLALFCNVKKEAVIQ
SIDASTIYEVPNLMLEEGLDVVALKKLDLPKKASPDLKNWNTFLKRLKSPKQTVNIGLVGKYVEMQDCYKSILEAFIHAG
AANETKVNVISIHSEHINADNVEEKLGTLDGVLVAPGFGERGIEGKIEAVRYVRENNIPFFGICLGMQMSVIEYSRNILG
YADANSTEMNEKTPHPVVNLMEEQKNITDKGGTMRLGAWKCDIKPNTLAHRIYGEKTISERHRHRYEYNNKYADELQKAG
LKASGVNPDTGLVEIVELENHPFFIGVQYHPEYKSTVANPHPIFVNFVAAAVNAHKK
>Mature_537_residues
MNQTKYIFVTGGVTSSLGKGIIAASLAKLLQGRGYRTTIQKFDPYINVDPGTLNPYEHGECYVTDDGAETDLDLGHYERF
LNVPTSQANNVTTGRVYLSVIEKERRGEFLGKTVQVVPHITNEIKDRMQLLGKSGDYDIVITEIGGTVGDIESLPYIESV
RQLVWELGENNGIVIHLTLVPYLAAAGELKTKPTQHSVKTLMESGIKADILVCRTEHELSQELRQKLALFCNVKKEAVIQ
SIDASTIYEVPNLMLEEGLDVVALKKLDLPKKASPDLKNWNTFLKRLKSPKQTVNIGLVGKYVEMQDCYKSILEAFIHAG
AANETKVNVISIHSEHINADNVEEKLGTLDGVLVAPGFGERGIEGKIEAVRYVRENNIPFFGICLGMQMSVIEYSRNILG
YADANSTEMNEKTPHPVVNLMEEQKNITDKGGTMRLGAWKCDIKPNTLAHRIYGEKTISERHRHRYEYNNKYADELQKAG
LKASGVNPDTGLVEIVELENHPFFIGVQYHPEYKSTVANPHPIFVNFVAAAVNAHKK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=548, Percent_Identity=45.0729927007299, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI28559085, Length=547, Percent_Identity=42.7787934186472, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI28559083, Length=547, Percent_Identity=42.7787934186472, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI221316689, Length=547, Percent_Identity=42.7787934186472, Blast_Score=456, Evalue=1e-128,
Organism=Escherichia coli, GI1789142, Length=544, Percent_Identity=50.9191176470588, Blast_Score=549, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI25148299, Length=602, Percent_Identity=36.8770764119601, Blast_Score=403, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6322563, Length=559, Percent_Identity=40.7871198568873, Blast_Score=435, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6319432, Length=561, Percent_Identity=40.6417112299465, Blast_Score=423, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24664469, Length=549, Percent_Identity=43.5336976320583, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI21357815, Length=494, Percent_Identity=41.7004048582996, Blast_Score=384, Evalue=1e-107,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_FLAJ1 (A5FHA4)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001194729.1
- ProteinModelPortal:   A5FHA4
- SMR:   A5FHA4
- STRING:   A5FHA4
- GeneID:   5092115
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_2383
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   TEWLRGN
- ProtClustDB:   PRK05380
- BioCyc:   FJOH376686:FJOH_2383-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 59809; Mature: 59809

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS51273 GATASE_TYPE_1

Important sites: ACT_SITE 384-384 ACT_SITE 510-510 ACT_SITE 512-512

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQTKYIFVTGGVTSSLGKGIIAASLAKLLQGRGYRTTIQKFDPYINVDPGTLNPYEHGE
CCCCEEEEEECCCHHHHCCHHHHHHHHHHHHCCCCEEHHHHCCCEEECCCCCCCCCCCCE
CYVTDDGAETDLDLGHYERFLNVPTSQANNVTTGRVYLSVIEKERRGEFLGKTVQVVPHI
EEEECCCCCCCCCHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHH
TNEIKDRMQLLGKSGDYDIVITEIGGTVGDIESLPYIESVRQLVWELGENNGIVIHLTLV
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHCCHHHHHHHHHHHHHCCCCCEEEEEEHH
PYLAAAGELKTKPTQHSVKTLMESGIKADILVCRTEHELSQELRQKLALFCNVKKEAVIQ
HHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHH
SIDASTIYEVPNLMLEEGLDVVALKKLDLPKKASPDLKNWNTFLKRLKSPKQTVNIGLVG
HCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEE
KYVEMQDCYKSILEAFIHAGAANETKVNVISIHSEHINADNVEEKLGTLDGVLVAPGFGE
CHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHCCCCCEEECCCCCC
RGIEGKIEAVRYVRENNIPFFGICLGMQMSVIEYSRNILGYADANSTEMNEKTPHPVVNL
CCCCCHHHHHHHHHHCCCCEEEEEHHCHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHH
MEEQKNITDKGGTMRLGAWKCDIKPNTLAHRIYGEKTISERHRHRYEYNNKYADELQKAG
HHHHCCCCCCCCEEEEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHCC
LKASGVNPDTGLVEIVELENHPFFIGVQYHPEYKSTVANPHPIFVNFVAAAVNAHKK
CCCCCCCCCCCCEEEEEECCCCEEEEEEECCCHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNQTKYIFVTGGVTSSLGKGIIAASLAKLLQGRGYRTTIQKFDPYINVDPGTLNPYEHGE
CCCCEEEEEECCCHHHHCCHHHHHHHHHHHHCCCCEEHHHHCCCEEECCCCCCCCCCCCE
CYVTDDGAETDLDLGHYERFLNVPTSQANNVTTGRVYLSVIEKERRGEFLGKTVQVVPHI
EEEECCCCCCCCCHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHH
TNEIKDRMQLLGKSGDYDIVITEIGGTVGDIESLPYIESVRQLVWELGENNGIVIHLTLV
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHCCHHHHHHHHHHHHHCCCCCEEEEEEHH
PYLAAAGELKTKPTQHSVKTLMESGIKADILVCRTEHELSQELRQKLALFCNVKKEAVIQ
HHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHH
SIDASTIYEVPNLMLEEGLDVVALKKLDLPKKASPDLKNWNTFLKRLKSPKQTVNIGLVG
HCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEE
KYVEMQDCYKSILEAFIHAGAANETKVNVISIHSEHINADNVEEKLGTLDGVLVAPGFGE
CHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHCCCCCEEECCCCCC
RGIEGKIEAVRYVRENNIPFFGICLGMQMSVIEYSRNILGYADANSTEMNEKTPHPVVNL
CCCCCHHHHHHHHHHCCCCEEEEEHHCHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHH
MEEQKNITDKGGTMRLGAWKCDIKPNTLAHRIYGEKTISERHRHRYEYNNKYADELQKAG
HHHHCCCCCCCCEEEEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHCC
LKASGVNPDTGLVEIVELENHPFFIGVQYHPEYKSTVANPHPIFVNFVAAAVNAHKK
CCCCCCCCCCCCEEEEEECCCCEEEEEEECCCHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA