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The map label for this gene is topB [H]

Identifier: 146300044

GI number: 146300044

Start: 2763305

End: 2765611

Strand: Reverse

Name: topB [H]

Synonym: Fjoh_2289

Alternate gene names: 146300044

Gene position: 2765611-2763305 (Counterclockwise)

Preceding gene: 146300045

Following gene: 146300043

Centisome position: 45.36

GC content: 37.88

Gene sequence:

>2307_bases
ATGAAGGTATGTATTGCTGAGAAACCAAGTGTAGCACGTGAAATCGCATCTGTTTTGGGCGCCAATACCAAGCACGACGG
ATATTACGAAGGGAACGGATATGCCGTAACCTACACTTTTGGGCATTTATGCACCTTAAAAGAACCTAACGATTACAAAC
CGCACTGGAAAAGCTGGGATTTAAACAATCTGCCCATGCTTCCTGAAAAATTCGAAACCAAAGTTGTTCAGAATTCGGGA
ATCCAGAAACAGTTTAAAATCATCAAAACACTTTTTGATAAAGCCGAATTAGTCATAAACTGCGGGGATGCCGGGCAAGA
AGGAGAATTAATCCAGCGCTGGGTAATGAACGAAGCGCATTACAAAGGCGAAATAAAACGTTTATGGATTTCTTCCTTAA
CCACCGAAGCCATAAAAGAAGGTTTTGACAACCTGAAACCTTCTGAAAACTATGATAATTTATTCTACGCCGGATTTTCG
AGAGCCATTGGCGACTGGCTTCTAGGGATGAATGCAACTCGTTTGTATACCGTAAAACATGGCGGTTACAAACAAGTTTT
GTCTATTGGACGCGTACAAACACCAACACTGGCAATGGTTGTAGACCGATTTAAAGAAATCGAAAACTTTAAACCGCAGC
CGTATTGGGAATTGCAGACTTTATACAGAGAAACGCTTTTTAGTTATGAAGAAGGGCGTTTTCTAAAAAAAGAAGACGGA
GAAATTCTGGCTGAAAAAGTAAAGGAAAGTGATTTTGAAATTGTTTCTGTTGATAAAAAAAACGGAAACGAATATGCTCC
AAAACTATTTGACTTGACAGGTTTACAGGTGTATTGCAACCAAAAATTTGGATTTTCGGCAGAAGAAACGCTTAAAATTG
CGCAGACTTTATACGAACAAAAAGCCATTACTTATCCCAGAGTTGATACCACTTTTCTGCCTGGAGATATTTATCCAAAA
GTACCAGGGATTCTGCAAAAACTGACTAATTATAGTCATTTAACAGAACCGCTTTTAGGAAAAAAAATTAAAAAATCGCC
TAAAGTTTTCAACGACAAAAAAGTAACAGATCACCATGCAATTATTCCAACCGGAATAGAAATTAATCTGCCTTATAATC
AGCAGCAAGTTTACGATATAATTGTAAAACGTTTTCTTGCTGTATTTTACGACGATTGTCTTGTTGCCAACACAACTGTA
ATTGGAAAAGCTGCCGACGTAACTTTTAAAGCGACAGGAAAAGAAATCTTAAAGAAAGGTTTCCGTGTGGTTTTTGAAGA
TCCGAATGCGAAAGAAAAAGAACCGGATTTACTGCCGAGTTTTGTTGTGGGGGAAAAAGGACCGCATGAACCTTCATTTC
TTCAAAAAGAAACCAAACCGCCTAATCATTTTACCGAAGCAACTTTGCTGCGTGCCATGGAAACCGCAGGAAAACAAGTA
GACGACGAAGATTTACGCGAATTGATGAAAGAAAACGGAATTGGTCGTCCGTCTACACGAGCGAACATTATCGAAACGCT
TTTTAAACGTCAGTATATTGTTCGAAATAAAAAACAGGTTTTACCAACTACAACAGGAATTCAGTTGATTGATACGATTC
AGAATGAATTGATAAAATCGGCTGAGCTTACAGGTTCGTGGGAAAAGCAATTGAAAGATATCGAAAAAGGTACTTTTACA
GCAGCTGCTTTTATCAGAAACATGAAACGCATGGTCGAAGCTTTGGTTACCGAAGTACGAAGTGAAACCAGACACGCCAA
TATTTCGCATGCTGCAACGATCCAGAAACCAGCTGTAAAAGCGGAGAAAAAGAAAGCCGCAGGAATTTTGGCAGAAACTT
GTCCGAAATGCCAGAAAGGAAATTTTATAAAAGGGAAATCGGCTTTTGGGTGTTCTGAATATAAATCGGGATGCGATTTT
GTACTTCCTTATGGTTTTCATGATAAAAAAATTACCGAAAGCCAATATTTACGATTAGTTCAAAAAGGCTCAACCGTAAA
CATAAAAGGTTTTAAAACCGATGCCGGAACAGTTGAAGGTTTAATTCGTTTTGAAGAGAATTACAAACTTAAACTTGAGC
CTAAAAAAACGTATATAAAAAAGACAACTAAGGAAACAGCATCAGATTCTTTATCCTGCCCAAAATGCAAAAAAGGAAAC
ATCTTAAAAGGAAAAACCGCTTACGGATGTTCTGAATATAAATCGGGTTGTGATTTTAAAGTTACTTTTGATGAAGTCCG
CGCAAAACTTCAAGATCAAAAACCAACAAAGGAATTGGTCTACTCTATTTTAGAGGGAAGCGTTTAG

Upstream 100 bases:

>100_bases
TGTTTGTGGTTTTTATGTTTTATTGCCCCTTTGTAACTAAGAACCTTTGTCCCTTTCTCATAAAAATCCTAACTTCGCTC
TTCAATAAGAAAAGGAGAAA

Downstream 100 bases:

>100_bases
TTTTTCTTGTCACAGATTAAAGGGATTAAAATGATTTTTGACTGTGTTTTTTTGCCACTAATTATACGAATTCTCACTAA
TTCATTTTTACACGCAGATT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 768; Mature: 768

Protein sequence:

>768_residues
MKVCIAEKPSVAREIASVLGANTKHDGYYEGNGYAVTYTFGHLCTLKEPNDYKPHWKSWDLNNLPMLPEKFETKVVQNSG
IQKQFKIIKTLFDKAELVINCGDAGQEGELIQRWVMNEAHYKGEIKRLWISSLTTEAIKEGFDNLKPSENYDNLFYAGFS
RAIGDWLLGMNATRLYTVKHGGYKQVLSIGRVQTPTLAMVVDRFKEIENFKPQPYWELQTLYRETLFSYEEGRFLKKEDG
EILAEKVKESDFEIVSVDKKNGNEYAPKLFDLTGLQVYCNQKFGFSAEETLKIAQTLYEQKAITYPRVDTTFLPGDIYPK
VPGILQKLTNYSHLTEPLLGKKIKKSPKVFNDKKVTDHHAIIPTGIEINLPYNQQQVYDIIVKRFLAVFYDDCLVANTTV
IGKAADVTFKATGKEILKKGFRVVFEDPNAKEKEPDLLPSFVVGEKGPHEPSFLQKETKPPNHFTEATLLRAMETAGKQV
DDEDLRELMKENGIGRPSTRANIIETLFKRQYIVRNKKQVLPTTTGIQLIDTIQNELIKSAELTGSWEKQLKDIEKGTFT
AAAFIRNMKRMVEALVTEVRSETRHANISHAATIQKPAVKAEKKKAAGILAETCPKCQKGNFIKGKSAFGCSEYKSGCDF
VLPYGFHDKKITESQYLRLVQKGSTVNIKGFKTDAGTVEGLIRFEENYKLKLEPKKTYIKKTTKETASDSLSCPKCKKGN
ILKGKTAYGCSEYKSGCDFKVTFDEVRAKLQDQKPTKELVYSILEGSV

Sequences:

>Translated_768_residues
MKVCIAEKPSVAREIASVLGANTKHDGYYEGNGYAVTYTFGHLCTLKEPNDYKPHWKSWDLNNLPMLPEKFETKVVQNSG
IQKQFKIIKTLFDKAELVINCGDAGQEGELIQRWVMNEAHYKGEIKRLWISSLTTEAIKEGFDNLKPSENYDNLFYAGFS
RAIGDWLLGMNATRLYTVKHGGYKQVLSIGRVQTPTLAMVVDRFKEIENFKPQPYWELQTLYRETLFSYEEGRFLKKEDG
EILAEKVKESDFEIVSVDKKNGNEYAPKLFDLTGLQVYCNQKFGFSAEETLKIAQTLYEQKAITYPRVDTTFLPGDIYPK
VPGILQKLTNYSHLTEPLLGKKIKKSPKVFNDKKVTDHHAIIPTGIEINLPYNQQQVYDIIVKRFLAVFYDDCLVANTTV
IGKAADVTFKATGKEILKKGFRVVFEDPNAKEKEPDLLPSFVVGEKGPHEPSFLQKETKPPNHFTEATLLRAMETAGKQV
DDEDLRELMKENGIGRPSTRANIIETLFKRQYIVRNKKQVLPTTTGIQLIDTIQNELIKSAELTGSWEKQLKDIEKGTFT
AAAFIRNMKRMVEALVTEVRSETRHANISHAATIQKPAVKAEKKKAAGILAETCPKCQKGNFIKGKSAFGCSEYKSGCDF
VLPYGFHDKKITESQYLRLVQKGSTVNIKGFKTDAGTVEGLIRFEENYKLKLEPKKTYIKKTTKETASDSLSCPKCKKGN
ILKGKTAYGCSEYKSGCDFKVTFDEVRAKLQDQKPTKELVYSILEGSV
>Mature_768_residues
MKVCIAEKPSVAREIASVLGANTKHDGYYEGNGYAVTYTFGHLCTLKEPNDYKPHWKSWDLNNLPMLPEKFETKVVQNSG
IQKQFKIIKTLFDKAELVINCGDAGQEGELIQRWVMNEAHYKGEIKRLWISSLTTEAIKEGFDNLKPSENYDNLFYAGFS
RAIGDWLLGMNATRLYTVKHGGYKQVLSIGRVQTPTLAMVVDRFKEIENFKPQPYWELQTLYRETLFSYEEGRFLKKEDG
EILAEKVKESDFEIVSVDKKNGNEYAPKLFDLTGLQVYCNQKFGFSAEETLKIAQTLYEQKAITYPRVDTTFLPGDIYPK
VPGILQKLTNYSHLTEPLLGKKIKKSPKVFNDKKVTDHHAIIPTGIEINLPYNQQQVYDIIVKRFLAVFYDDCLVANTTV
IGKAADVTFKATGKEILKKGFRVVFEDPNAKEKEPDLLPSFVVGEKGPHEPSFLQKETKPPNHFTEATLLRAMETAGKQV
DDEDLRELMKENGIGRPSTRANIIETLFKRQYIVRNKKQVLPTTTGIQLIDTIQNELIKSAELTGSWEKQLKDIEKGTFT
AAAFIRNMKRMVEALVTEVRSETRHANISHAATIQKPAVKAEKKKAAGILAETCPKCQKGNFIKGKSAFGCSEYKSGCDF
VLPYGFHDKKITESQYLRLVQKGSTVNIKGFKTDAGTVEGLIRFEENYKLKLEPKKTYIKKTTKETASDSLSCPKCKKGN
ILKGKTAYGCSEYKSGCDFKVTFDEVRAKLQDQKPTKELVYSILEGSV

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=599, Percent_Identity=25.8764607679466, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI10835218, Length=677, Percent_Identity=24.9630723781388, Blast_Score=137, Evalue=5e-32,
Organism=Escherichia coli, GI1788061, Length=593, Percent_Identity=32.5463743676223, Blast_Score=312, Evalue=5e-86,
Organism=Escherichia coli, GI1787529, Length=561, Percent_Identity=25.3119429590018, Blast_Score=123, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI32563869, Length=569, Percent_Identity=26.5377855887522, Blast_Score=148, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17555378, Length=605, Percent_Identity=24.9586776859504, Blast_Score=129, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6323263, Length=610, Percent_Identity=24.9180327868852, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24640096, Length=574, Percent_Identity=26.3066202090592, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24585251, Length=599, Percent_Identity=25.8764607679466, Blast_Score=121, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 86950; Mature: 86950

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVCIAEKPSVAREIASVLGANTKHDGYYEGNGYAVTYTFGHLCTLKEPNDYKPHWKSWD
CCEECCCCCHHHHHHHHHHCCCCCCCCEEECCCEEEEEEECCEEEECCCCCCCCCCCCCC
LNNLPMLPEKFETKVVQNSGIQKQFKIIKTLFDKAELVINCGDAGQEGELIQRWVMNEAH
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHH
YKGEIKRLWISSLTTEAIKEGFDNLKPSENYDNLFYAGFSRAIGDWLLGMNATRLYTVKH
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEEC
GGYKQVLSIGRVQTPTLAMVVDRFKEIENFKPQPYWELQTLYRETLFSYEEGRFLKKEDG
CCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCH
EILAEKVKESDFEIVSVDKKNGNEYAPKLFDLTGLQVYCNQKFGFSAEETLKIAQTLYEQ
HHHHHHHHCCCCEEEEEECCCCCCCCCHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
KAITYPRVDTTFLPGDIYPKVPGILQKLTNYSHLTEPLLGKKIKKSPKVFNDKKVTDHHA
HCCCCCCCCCEECCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
IIPTGIEINLPYNQQQVYDIIVKRFLAVFYDDCLVANTTVIGKAADVTFKATGKEILKKG
EEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEECCCHHHHHCC
FRVVFEDPNAKEKEPDLLPSFVVGEKGPHEPSFLQKETKPPNHFTEATLLRAMETAGKQV
CEEEECCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
DDEDLRELMKENGIGRPSTRANIIETLFKRQYIVRNKKQVLPTTTGIQLIDTIQNELIKS
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
AELTGSWEKQLKDIEKGTFTAAAFIRNMKRMVEALVTEVRSETRHANISHAATIQKPAVK
HHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHH
AEKKKAAGILAETCPKCQKGNFIKGKSAFGCSEYKSGCDFVLPYGFHDKKITESQYLRLV
HHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHCCCCCEEEECCCCCCCCCHHHHHHHH
QKGSTVNIKGFKTDAGTVEGLIRFEENYKLKLEPKKTYIKKTTKETASDSLSCPKCKKGN
HCCCEEEEEEECCCCHHHHHHEEECCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCC
ILKGKTAYGCSEYKSGCDFKVTFDEVRAKLQDQKPTKELVYSILEGSV
EECCCCCCCHHHHCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKVCIAEKPSVAREIASVLGANTKHDGYYEGNGYAVTYTFGHLCTLKEPNDYKPHWKSWD
CCEECCCCCHHHHHHHHHHCCCCCCCCEEECCCEEEEEEECCEEEECCCCCCCCCCCCCC
LNNLPMLPEKFETKVVQNSGIQKQFKIIKTLFDKAELVINCGDAGQEGELIQRWVMNEAH
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHH
YKGEIKRLWISSLTTEAIKEGFDNLKPSENYDNLFYAGFSRAIGDWLLGMNATRLYTVKH
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEEC
GGYKQVLSIGRVQTPTLAMVVDRFKEIENFKPQPYWELQTLYRETLFSYEEGRFLKKEDG
CCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCH
EILAEKVKESDFEIVSVDKKNGNEYAPKLFDLTGLQVYCNQKFGFSAEETLKIAQTLYEQ
HHHHHHHHCCCCEEEEEECCCCCCCCCHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
KAITYPRVDTTFLPGDIYPKVPGILQKLTNYSHLTEPLLGKKIKKSPKVFNDKKVTDHHA
HCCCCCCCCCEECCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
IIPTGIEINLPYNQQQVYDIIVKRFLAVFYDDCLVANTTVIGKAADVTFKATGKEILKKG
EEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEECCCHHHHHCC
FRVVFEDPNAKEKEPDLLPSFVVGEKGPHEPSFLQKETKPPNHFTEATLLRAMETAGKQV
CEEEECCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
DDEDLRELMKENGIGRPSTRANIIETLFKRQYIVRNKKQVLPTTTGIQLIDTIQNELIKS
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
AELTGSWEKQLKDIEKGTFTAAAFIRNMKRMVEALVTEVRSETRHANISHAATIQKPAVK
HHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHH
AEKKKAAGILAETCPKCQKGNFIKGKSAFGCSEYKSGCDFVLPYGFHDKKITESQYLRLV
HHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHCCCCCEEEECCCCCCCCCHHHHHHHH
QKGSTVNIKGFKTDAGTVEGLIRFEENYKLKLEPKKTYIKKTTKETASDSLSCPKCKKGN
HCCCEEEEEEECCCCHHHHHHEEECCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCC
ILKGKTAYGCSEYKSGCDFKVTFDEVRAKLQDQKPTKELVYSILEGSV
EECCCCCCCHHHHCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA