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The map label for this gene is pyrD

Identifier: 146299966

GI number: 146299966

Start: 2664399

End: 2665433

Strand: Direct

Name: pyrD

Synonym: Fjoh_2210

Alternate gene names: 146299966

Gene position: 2664399-2665433 (Clockwise)

Preceding gene: 146299959

Following gene: 146299969

Centisome position: 43.7

GC content: 34.3

Gene sequence:

>1035_bases
ATGTATAAATTGATAATTCGTCCGATACTTTTTTGGTTTGATCCTGAAGAAGTGCATTACTTTACTTTTTCATTTGTAAA
ATTCATTTCAAAAATCCCTGGAGTTTCGTCGATTATAAGATCAATTTATGAAGTAAAAGACAGCCGTTTAGAAAGAGAAG
TTTTTGGTATTAAATTTAAAAATCCGGTTGGGCTTGCAGCAGGATTTGATAAAGATGCTAAACTGTATAAAGAGCTTTCA
GATTTTGGTTTCGGTTTTATAGAAATTGGAACCGTAACTCCGGTTGGTCAGGAAGGAAATCCTAAAAAACGTTTGTTTCG
CTTAAAAGAAGATCAGGCAATTATTAACCGAATGGGGTTTAATAACGGCGGAGTTCTCGAAGCAGTAGAACGTTTGAAGA
AAAATTCGGGAGTTTTAATTGGAGGAAATATCGGAAAGAACAAAGTAACCGACAACGAAGATGCTGTTAAAGATTACATC
ATTTGTTTTGACGCTTTATACAATCATGTTGATTATTTTGTTGTGAATGTAAGTTCGCCAAATACTCCAAATTTGAGAGC
ATTACAGGATAAAGAACCTCTAACTGCTTTATTGCAGACTTTGCAGAACAGAAATGTTGAAAAACAAAAAACAAGTACTC
AAAAAGTAAAACCAATTCTTCTAAAAATTGCCCCAGACCTTACAGATGAGCAATTGTTAGATATCATTGATATTGTAAAA
ACAACGCAGATTGCAGGTGTAATTGCAACCAATACTACAATTTCGAGAGAAGGTTTACAATCTTCAAATCAATCTGAAAC
GGGAGGTTTATCTGGAAAACCATTAACAAAACGCTCTACAGAAGTAATTCGTTTTCTTTCAGAAAAAAGCAACAAAGCAT
TCCCAATTATTGGGGTAGGAGGAATTCATTCTGCCGACGATGCGATTGAAAAACTAAATGCAGGAGCAAGTTTAGTACAA
TTGTACACAGGATTTATTTACGAAGGACCAGCGTTGATAAAAGCAATCAACAAAAAAGTTTTAGGGCAGTTGTAA

Upstream 100 bases:

>100_bases
TATAACTTGCAACCTCTTATTTATTTGTTTTTTCTAAAAAAGCACCTTTTTAAACTATAATATTTAAATTTGCAGCCAAA
ATAACAACAACACAACTCTT

Downstream 100 bases:

>100_bases
AAGAAGTGCCTGAACGATTAATCCGGCGAGAATTGCGGCTCCAAAACTGATTAAAGTACCAATTAAAACATATTCGGTTA
GTTTTCGGTCTTTGGCTTCT

Product: dihydroorotate dehydrogenase 2

Products: NA

Alternate protein names: DHOdehase; DHOD; DHODase; Dihydroorotate oxidase

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MYKLIIRPILFWFDPEEVHYFTFSFVKFISKIPGVSSIIRSIYEVKDSRLEREVFGIKFKNPVGLAAGFDKDAKLYKELS
DFGFGFIEIGTVTPVGQEGNPKKRLFRLKEDQAIINRMGFNNGGVLEAVERLKKNSGVLIGGNIGKNKVTDNEDAVKDYI
ICFDALYNHVDYFVVNVSSPNTPNLRALQDKEPLTALLQTLQNRNVEKQKTSTQKVKPILLKIAPDLTDEQLLDIIDIVK
TTQIAGVIATNTTISREGLQSSNQSETGGLSGKPLTKRSTEVIRFLSEKSNKAFPIIGVGGIHSADDAIEKLNAGASLVQ
LYTGFIYEGPALIKAINKKVLGQL

Sequences:

>Translated_344_residues
MYKLIIRPILFWFDPEEVHYFTFSFVKFISKIPGVSSIIRSIYEVKDSRLEREVFGIKFKNPVGLAAGFDKDAKLYKELS
DFGFGFIEIGTVTPVGQEGNPKKRLFRLKEDQAIINRMGFNNGGVLEAVERLKKNSGVLIGGNIGKNKVTDNEDAVKDYI
ICFDALYNHVDYFVVNVSSPNTPNLRALQDKEPLTALLQTLQNRNVEKQKTSTQKVKPILLKIAPDLTDEQLLDIIDIVK
TTQIAGVIATNTTISREGLQSSNQSETGGLSGKPLTKRSTEVIRFLSEKSNKAFPIIGVGGIHSADDAIEKLNAGASLVQ
LYTGFIYEGPALIKAINKKVLGQL
>Mature_344_residues
MYKLIIRPILFWFDPEEVHYFTFSFVKFISKIPGVSSIIRSIYEVKDSRLEREVFGIKFKNPVGLAAGFDKDAKLYKELS
DFGFGFIEIGTVTPVGQEGNPKKRLFRLKEDQAIINRMGFNNGGVLEAVERLKKNSGVLIGGNIGKNKVTDNEDAVKDYI
ICFDALYNHVDYFVVNVSSPNTPNLRALQDKEPLTALLQTLQNRNVEKQKTSTQKVKPILLKIAPDLTDEQLLDIIDIVK
TTQIAGVIATNTTISREGLQSSNQSETGGLSGKPLTKRSTEVIRFLSEKSNKAFPIIGVGGIHSADDAIEKLNAGASLVQ
LYTGFIYEGPALIKAINKKVLGQL

Specific function: Pyrimidine biosynthesis; fourth step. [C]

COG id: COG0167

COG function: function code F; Dihydroorotate dehydrogenase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily

Homologues:

Organism=Homo sapiens, GI45006951, Length=339, Percent_Identity=45.7227138643068, Blast_Score=259, Evalue=3e-69,
Organism=Escherichia coli, GI1787177, Length=339, Percent_Identity=49.5575221238938, Blast_Score=292, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17509475, Length=307, Percent_Identity=47.2312703583062, Blast_Score=254, Evalue=4e-68,
Organism=Drosophila melanogaster, GI281361352, Length=344, Percent_Identity=43.0232558139535, Blast_Score=257, Evalue=8e-69,
Organism=Drosophila melanogaster, GI17137316, Length=344, Percent_Identity=43.0232558139535, Blast_Score=257, Evalue=8e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRD_FLAJ1 (A5FHT2)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001194557.1
- ProteinModelPortal:   A5FHT2
- SMR:   A5FHT2
- STRING:   A5FHT2
- GeneID:   5092216
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_2210
- eggNOG:   COG0167
- HOGENOM:   HBG351027
- OMA:   SYVTVNI
- ProtClustDB:   PRK05286
- BioCyc:   FJOH376686:FJOH_2210-MONOMER
- HAMAP:   MF_00225
- InterPro:   IPR013785
- InterPro:   IPR012135
- InterPro:   IPR005719
- InterPro:   IPR001295
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000164
- TIGRFAMs:   TIGR01036

Pfam domain/function: PF01180 DHO_dh

EC number: =1.3.5.2

Molecular weight: Translated: 38113; Mature: 38113

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS00911 DHODEHASE_1; PS00912 DHODEHASE_2

Important sites: ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKLIIRPILFWFDPEEVHYFTFSFVKFISKIPGVSSIIRSIYEVKDSRLEREVFGIKFK
CHHHHEEHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCEEEC
NPVGLAAGFDKDAKLYKELSDFGFGFIEIGTVTPVGQEGNPKKRLFRLKEDQAIINRMGF
CCCCEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
NNGGVLEAVERLKKNSGVLIGGNIGKNKVTDNEDAVKDYIICFDALYNHVDYFVVNVSSP
CCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCC
NTPNLRALQDKEPLTALLQTLQNRNVEKQKTSTQKVKPILLKIAPDLTDEQLLDIIDIVK
CCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHH
TTQIAGVIATNTTISREGLQSSNQSETGGLSGKPLTKRSTEVIRFLSEKSNKAFPIIGVG
HHHHHEEEEECCEECHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEC
GIHSADDAIEKLNAGASLVQLYTGFIYEGPALIKAINKKVLGQL
CCCCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYKLIIRPILFWFDPEEVHYFTFSFVKFISKIPGVSSIIRSIYEVKDSRLEREVFGIKFK
CHHHHEEHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCEEEC
NPVGLAAGFDKDAKLYKELSDFGFGFIEIGTVTPVGQEGNPKKRLFRLKEDQAIINRMGF
CCCCEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
NNGGVLEAVERLKKNSGVLIGGNIGKNKVTDNEDAVKDYIICFDALYNHVDYFVVNVSSP
CCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCC
NTPNLRALQDKEPLTALLQTLQNRNVEKQKTSTQKVKPILLKIAPDLTDEQLLDIIDIVK
CCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHH
TTQIAGVIATNTTISREGLQSSNQSETGGLSGKPLTKRSTEVIRFLSEKSNKAFPIIGVG
HHHHHEEEEECCEECHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEC
GIHSADDAIEKLNAGASLVQLYTGFIYEGPALIKAINKKVLGQL
CCCCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA