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The map label for this gene is murD [H]

Identifier: 146299568

GI number: 146299568

Start: 2089613

End: 2090944

Strand: Direct

Name: murD [H]

Synonym: Fjoh_1808

Alternate gene names: 146299568

Gene position: 2089613-2090944 (Clockwise)

Preceding gene: 146299567

Following gene: 146299569

Centisome position: 34.27

GC content: 35.89

Gene sequence:

>1332_bases
ATGAGATTAGTGGTATTAGGTGGAGGAGAAAGCGGGGTTGGAACCGCTATTCTCGGGAAGAAAAAGGGATACGACGTTTT
TGTATCGGATTTTGGAAAGATAAAAGAGAGTTATAAAGAAGTTCTTATCATTAATAAGATTGACTGGGAAGAAGAACAGC
ATACTGAAGATTTGATTTTGAATGCTGATGTGGTGATGAAAAGCCCCGGAATTCCGGATAAATCTCCGATAATAAAAAAA
CTGGTTGCGGCGGGAGTAAAAGTGATTTCGGAAATAGAATTTGCAAAACCTTTTACAGAAGCATTAACGATAGGAATTAC
CGGAAGTAATGGTAAAACGACAACCACAATGCTTACGCATCATTTACTAAAATCGGCCGGACTCAATGTAGGTTTGGGAG
GAAATATAGGAAAGAGTTTTGCCTGGCAGGTAGCCGAAAATAAATACGACGCATACGTTCTTGAATTAAGCAGTTTTCAG
TTAGACGGAATAATAGATTACCGGCCGGATATCGCCATAATAACCAATATCAGCCCGGATCATTTAGATCGATACGAATA
TAAATATGAAAATTATATCAATTCGAAATTTCGAATAACGATGAACCAAACCGAAAGTGATTATCTCATTTACGATGCAG
ACGATGAGGCAAGCACAGAATGGTTAAAAAACAACAAAACAAAAGCAAAATTAATTCCTTTTTCATTGACCAAAACATTC
GATGAAGGGGCTTCTATAAATAACAACAAAATGGAAATAAAGATCAACCAAGAAGAGTTTACAATGGAAACAGAACACAT
TGCGTTAGAAGGAAAACATAATATGAAAAACGCAATGGCAGCAAGCTCTGTAGCAAAATTGATGCAAATTAGAAATGCAA
CGATTCGTGAAAGTTTATCTAATTTTCAAGGTGTTGAACACCGTTTAGAAAAAGTATTAAAAATTCAGAATGTACAATAT
ATCAACGATTCAAAAGCAACAAATGTAAACGCTACTTTCTTTGCTTTAGATAGTATGAATGTTCCAACGGTTTGGATTGT
AGGAGGGGTTGATAAAGGAAACGATTACAATGAATTAATGTCATTAGTTCGCGAAAAAGTAAAAGCAATTATCTGCCTAG
GAATCGACAACCGTAAAATTATCGACGCTTTTGGAGCTGTAGTAGATATTATGGTTGAAGTAAATAACATGAATGATGCA
GTAAAAACTGCTCAAAGATTAACAGAAAAAGGTGATGCTGTTTTATTGTCTCCAGCCTGCGCGAGTTTCGATTTATTCGA
AAACTACGAAGACAGAGGGAAGCAATTCAAGCAAGCAGTTCATAACTTGTAG

Upstream 100 bases:

>100_bases
CCAGAAAAAAGGATACCACGAAAGTAAAATCGTAACCCGTTTCTGGATTGTTGCTGTAATGTTAGCCATATTATCAATCG
TTACTTTAAAACTAAGATAG

Downstream 100 bases:

>100_bases
AAAATAGTTTAAAGTTTCACGTTTCAGGTTTCAAGTTCGACACCTGAAACGTGAGGCCTGAAACAAAAAAACTTGAAACA
AAACAACAAATGAAGGAGCT

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MRLVVLGGGESGVGTAILGKKKGYDVFVSDFGKIKESYKEVLIINKIDWEEEQHTEDLILNADVVMKSPGIPDKSPIIKK
LVAAGVKVISEIEFAKPFTEALTIGITGSNGKTTTTMLTHHLLKSAGLNVGLGGNIGKSFAWQVAENKYDAYVLELSSFQ
LDGIIDYRPDIAIITNISPDHLDRYEYKYENYINSKFRITMNQTESDYLIYDADDEASTEWLKNNKTKAKLIPFSLTKTF
DEGASINNNKMEIKINQEEFTMETEHIALEGKHNMKNAMAASSVAKLMQIRNATIRESLSNFQGVEHRLEKVLKIQNVQY
INDSKATNVNATFFALDSMNVPTVWIVGGVDKGNDYNELMSLVREKVKAIICLGIDNRKIIDAFGAVVDIMVEVNNMNDA
VKTAQRLTEKGDAVLLSPACASFDLFENYEDRGKQFKQAVHNL

Sequences:

>Translated_443_residues
MRLVVLGGGESGVGTAILGKKKGYDVFVSDFGKIKESYKEVLIINKIDWEEEQHTEDLILNADVVMKSPGIPDKSPIIKK
LVAAGVKVISEIEFAKPFTEALTIGITGSNGKTTTTMLTHHLLKSAGLNVGLGGNIGKSFAWQVAENKYDAYVLELSSFQ
LDGIIDYRPDIAIITNISPDHLDRYEYKYENYINSKFRITMNQTESDYLIYDADDEASTEWLKNNKTKAKLIPFSLTKTF
DEGASINNNKMEIKINQEEFTMETEHIALEGKHNMKNAMAASSVAKLMQIRNATIRESLSNFQGVEHRLEKVLKIQNVQY
INDSKATNVNATFFALDSMNVPTVWIVGGVDKGNDYNELMSLVREKVKAIICLGIDNRKIIDAFGAVVDIMVEVNNMNDA
VKTAQRLTEKGDAVLLSPACASFDLFENYEDRGKQFKQAVHNL
>Mature_443_residues
MRLVVLGGGESGVGTAILGKKKGYDVFVSDFGKIKESYKEVLIINKIDWEEEQHTEDLILNADVVMKSPGIPDKSPIIKK
LVAAGVKVISEIEFAKPFTEALTIGITGSNGKTTTTMLTHHLLKSAGLNVGLGGNIGKSFAWQVAENKYDAYVLELSSFQ
LDGIIDYRPDIAIITNISPDHLDRYEYKYENYINSKFRITMNQTESDYLIYDADDEASTEWLKNNKTKAKLIPFSLTKTF
DEGASINNNKMEIKINQEEFTMETEHIALEGKHNMKNAMAASSVAKLMQIRNATIRESLSNFQGVEHRLEKVLKIQNVQY
INDSKATNVNATFFALDSMNVPTVWIVGGVDKGNDYNELMSLVREKVKAIICLGIDNRKIIDAFGAVVDIMVEVNNMNDA
VKTAQRLTEKGDAVLLSPACASFDLFENYEDRGKQFKQAVHNL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=406, Percent_Identity=32.0197044334975, Blast_Score=197, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 49402; Mature: 49402

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVVLGGGESGVGTAILGKKKGYDVFVSDFGKIKESYKEVLIINKIDWEEEQHTEDLIL
CEEEEEECCCCCCCEEEECCCCCCEEEHHHHHHHHHHHHHEEEEEECCCCCHHCCHHEEE
NADVVMKSPGIPDKSPIIKKLVAAGVKVISEIEFAKPFTEALTIGITGSNGKTTTTMLTH
ECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHH
HLLKSAGLNVGLGGNIGKSFAWQVAENKYDAYVLELSSFQLDGIIDYRPDIAIITNISPD
HHHHHCCCCCCCCCCCCCHHHHEECCCCCCEEEEEEECEEECCEEECCCCEEEEECCCHH
HLDRYEYKYENYINSKFRITMNQTESDYLIYDADDEASTEWLKNNKTKAKLIPFSLTKTF
HHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCHHHHHHHCCCCEEEEEEEEHHHHH
DEGASINNNKMEIKINQEEFTMETEHIALEGKHNMKNAMAASSVAKLMQIRNATIRESLS
CCCCCCCCCEEEEEECHHHHEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NFQGVEHRLEKVLKIQNVQYINDSKATNVNATFFALDSMNVPTVWIVGGVDKGNDYNELM
HHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHH
SLVREKVKAIICLGIDNRKIIDAFGAVVDIMVEVNNMNDAVKTAQRLTEKGDAVLLSPAC
HHHHHHHHEEEEEECCCCHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCEEEEECCC
ASFDLFENYEDRGKQFKQAVHNL
CCHHHHHCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLVVLGGGESGVGTAILGKKKGYDVFVSDFGKIKESYKEVLIINKIDWEEEQHTEDLIL
CEEEEEECCCCCCCEEEECCCCCCEEEHHHHHHHHHHHHHEEEEEECCCCCHHCCHHEEE
NADVVMKSPGIPDKSPIIKKLVAAGVKVISEIEFAKPFTEALTIGITGSNGKTTTTMLTH
ECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHH
HLLKSAGLNVGLGGNIGKSFAWQVAENKYDAYVLELSSFQLDGIIDYRPDIAIITNISPD
HHHHHCCCCCCCCCCCCCHHHHEECCCCCCEEEEEEECEEECCEEECCCCEEEEECCCHH
HLDRYEYKYENYINSKFRITMNQTESDYLIYDADDEASTEWLKNNKTKAKLIPFSLTKTF
HHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCHHHHHHHCCCCEEEEEEEEHHHHH
DEGASINNNKMEIKINQEEFTMETEHIALEGKHNMKNAMAASSVAKLMQIRNATIRESLS
CCCCCCCCCEEEEEECHHHHEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NFQGVEHRLEKVLKIQNVQYINDSKATNVNATFFALDSMNVPTVWIVGGVDKGNDYNELM
HHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHH
SLVREKVKAIICLGIDNRKIIDAFGAVVDIMVEVNNMNDAVKTAQRLTEKGDAVLLSPAC
HHHHHHHHEEEEEECCCCHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCEEEEECCC
ASFDLFENYEDRGKQFKQAVHNL
CCHHHHHCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA