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The map label for this gene is greA

Identifier: 146299350

GI number: 146299350

Start: 1841763

End: 1842239

Strand: Direct

Name: greA

Synonym: Fjoh_1590

Alternate gene names: 146299350

Gene position: 1841763-1842239 (Clockwise)

Preceding gene: 146299348

Following gene: 146299351

Centisome position: 30.21

GC content: 33.12

Gene sequence:

>477_bases
ATGAGTAAAGTATCTTATTATACCGCTGATGGGTTAAAAAAGTTAAAAGATGAATTGGAGCATTTAAAGAGTGTAATGCG
TCCTAAAGCATCTCAAGATATAGCAGACGCAAGAGACAAAGGCGATTTATCTGAAAATGCCGAGTACGATGCTGCAAAAG
AAGCGCAGGGTTTATTAGAAATGAGAATTGCTAAACTTGAAGAAGTATATGCAAATGCAAGATTAATTGATGAATCTCAA
TTAGATGTTTCTAAAGTTTTGGTTCTTTCTAATGTGAAAATTAAAAACCAAAGCAACGGAATGGAAATGAAATATACACT
TGTTGCAGAAAGTGAAGCTGATTTAAAAACAGGAAAAATATCTGTAACTTCTCCTATTGGAAAAGGACTGTTGGGAAAAT
CTGTTGGTGAAATTGCCGAAATTACAGTACCAAACGGAACTTTGAAATTCGAAATTCTTGAAATTACAAGAGAATAA

Upstream 100 bases:

>100_bases
TGATTAACATATAATTATGTACTATTAAAAATAATAGTATATTTGTAATACAAAATCCCGTCCCGATGGGATTTTTTCTA
TTTATATAAACGATGAAGTT

Downstream 100 bases:

>100_bases
ATTTTTTATAGTTTAACCGTTAAATCGGTTAAGCGGTTAACCAGTTAAACGAATAAACTATGAGTTCAATATTCACTAAA
ATAGTAAACGGAGAAATTCC

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSKVSYYTADGLKKLKDELEHLKSVMRPKASQDIADARDKGDLSENAEYDAAKEAQGLLEMRIAKLEEVYANARLIDESQ
LDVSKVLVLSNVKIKNQSNGMEMKYTLVAESEADLKTGKISVTSPIGKGLLGKSVGEIAEITVPNGTLKFEILEITRE

Sequences:

>Translated_158_residues
MSKVSYYTADGLKKLKDELEHLKSVMRPKASQDIADARDKGDLSENAEYDAAKEAQGLLEMRIAKLEEVYANARLIDESQ
LDVSKVLVLSNVKIKNQSNGMEMKYTLVAESEADLKTGKISVTSPIGKGLLGKSVGEIAEITVPNGTLKFEILEITRE
>Mature_157_residues
SKVSYYTADGLKKLKDELEHLKSVMRPKASQDIADARDKGDLSENAEYDAAKEAQGLLEMRIAKLEEVYANARLIDESQL
DVSKVLVLSNVKIKNQSNGMEMKYTLVAESEADLKTGKISVTSPIGKGLLGKSVGEIAEITVPNGTLKFEILEITRE

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=149, Percent_Identity=41.6107382550336, Blast_Score=116, Evalue=5e-28,
Organism=Escherichia coli, GI87082261, Length=145, Percent_Identity=30.3448275862069, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_FLAJ1 (A5FJJ8)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001193941.1
- ProteinModelPortal:   A5FJJ8
- SMR:   A5FJJ8
- STRING:   A5FJJ8
- GeneID:   5090941
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_1590
- eggNOG:   COG0782
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   FJOH376686:FJOH_1590-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17361; Mature: 17230

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVSYYTADGLKKLKDELEHLKSVMRPKASQDIADARDKGDLSENAEYDAAKEAQGLLE
CCCCCEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHH
MRIAKLEEVYANARLIDESQLDVSKVLVLSNVKIKNQSNGMEMKYTLVAESEADLKTGKI
HHHHHHHHHHHCCEECCCCCCCHHHHHHHHCEEEECCCCCCEEEEEEEECCCCCCCCCEE
SVTSPIGKGLLGKSVGEIAEITVPNGTLKFEILEITRE
EEECCCCCCHHHCCCCCEEEEEECCCCEEEEEEEEECC
>Mature Secondary Structure 
SKVSYYTADGLKKLKDELEHLKSVMRPKASQDIADARDKGDLSENAEYDAAKEAQGLLE
CCCCEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHH
MRIAKLEEVYANARLIDESQLDVSKVLVLSNVKIKNQSNGMEMKYTLVAESEADLKTGKI
HHHHHHHHHHHCCEECCCCCCCHHHHHHHHCEEEECCCCCCEEEEEEEECCCCCCCCCEE
SVTSPIGKGLLGKSVGEIAEITVPNGTLKFEILEITRE
EEECCCCCCHHHCCCCCEEEEEECCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA