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The map label for this gene is murF [H]

Identifier: 146299318

GI number: 146299318

Start: 1803065

End: 1804351

Strand: Direct

Name: murF [H]

Synonym: Fjoh_1558

Alternate gene names: 146299318

Gene position: 1803065-1804351 (Clockwise)

Preceding gene: 146299317

Following gene: 146299320

Centisome position: 29.57

GC content: 30.54

Gene sequence:

>1287_bases
ATGAATATTCAGGAAATTCATAACTTGTTTTTGAAATGCCAATCTCTTTCAATTGATACAAGAAAAATTGAAAAAGGTTC
TATGTTTTTTGCCATAAAAGGTGAAAATTTTGATGCAAATACTTTTGCTAAAGAAGCACTGGATTTAGGTGCATTATACG
TTGTTATAGATAACGAATCTTATTTTATTGACGACAGAACTATCTTAGTTAATAACAGCTTAGAAACACTGCAGGAACTG
TCGAAATTTCATCGTAATTATTTAAAACTTCCAATTGTTGCACTTACAGGAAGCAATGGTAAAACAACAACGAAAGAGCT
TATTAATGTTGTTTTGTCTAAGAAGTTTAAAACAAAAGCCACTATTGGAAATCTGAATAATCACATAGGTGTTCCGTTAA
CGCTGCTTTCTTTTACAAAAGAAACTGAAATTGGAATCGTAGAGATGGGAGCTAATCATAAAAAAGAAATAGAATTTTTA
TGTGAAATTGCCCAGCCGGATTATGGCTATATAACTAATTTCGGGAAAGCCCATTTAGAAGGTTTTGGCGGCGTGCAGGG
CGTTATTGAAGGGAAAAGTGAAATGTATCAATATTTGTTAAAAAACAATAAAACTGTTTTTGTAAATCTTGAAGATCCGA
TTCAGATAGAAAAATCAACCGGAATTAAAAACTTTACTTTTGGTGTAAACAAAGAATCTGCCGATTTAAAGATTAAAAAC
ATTCAGGCAAATCCTTTTGTGGTTATAGAATATGAAGATTTTAAAGTAGAATCGCATTTAATCGGGCTTTATAATGCGAA
TAACATCAATGCAGCAGTTTCGATAGGGAAATATTTCAATGTAGAAGATGCTTTAATAAAAGAAGCTATCGAGAATTATA
TTCCGGCAAATAATAGGTCTCAATTGCTAAAAAAAGGTTCAAATGAGATTATTTTAGATGCATATAATGCAAATCCAAGC
AGTATGGCTGTGGCGATTACTAATTTTCTGCAATTAGAAAATCAAAATAAAATAATGATTCTGGGTGATATGTTTGAACT
TGGAAATGAAAGCCATGAAGAACATAAAGCTATTGTAGATTCTCTGTCTAACCAGAATAAATCAAAATGTTATTTGATTG
GAAAATCATTTTATGAAAATAAGATTTCGAATGAAAATATTCAATTTTATGAAACATTTGATGCTTTCGCAGCTTATCTA
AAAACAATTGAATTTAAAGATAATACGATTTTGATAAAGGGCTCCAGAGGAATGGCTTTGGAACGCACTTTAGATTATAT
CTCATAA

Upstream 100 bases:

>100_bases
TCATAAAAAATTCCAAATTCCAAAGCTGCATTTTCAGTTCGGAATTTGGAATTTTTTTATTGCTTTTTTCTACTTTTATA
GTCTATAAAAAAGATATTAA

Downstream 100 bases:

>100_bases
AAAAAAGATCCGACATAAGTGTCGGATCTTTTTTTTAAATACTCATCAAGAAACCAATTAAGTTTTCTTTCTTATTCAGC
CCTAGCTTTTTTCTAAGTCG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNIQEIHNLFLKCQSLSIDTRKIEKGSMFFAIKGENFDANTFAKEALDLGALYVVIDNESYFIDDRTILVNNSLETLQEL
SKFHRNYLKLPIVALTGSNGKTTTKELINVVLSKKFKTKATIGNLNNHIGVPLTLLSFTKETEIGIVEMGANHKKEIEFL
CEIAQPDYGYITNFGKAHLEGFGGVQGVIEGKSEMYQYLLKNNKTVFVNLEDPIQIEKSTGIKNFTFGVNKESADLKIKN
IQANPFVVIEYEDFKVESHLIGLYNANNINAAVSIGKYFNVEDALIKEAIENYIPANNRSQLLKKGSNEIILDAYNANPS
SMAVAITNFLQLENQNKIMILGDMFELGNESHEEHKAIVDSLSNQNKSKCYLIGKSFYENKISNENIQFYETFDAFAAYL
KTIEFKDNTILIKGSRGMALERTLDYIS

Sequences:

>Translated_428_residues
MNIQEIHNLFLKCQSLSIDTRKIEKGSMFFAIKGENFDANTFAKEALDLGALYVVIDNESYFIDDRTILVNNSLETLQEL
SKFHRNYLKLPIVALTGSNGKTTTKELINVVLSKKFKTKATIGNLNNHIGVPLTLLSFTKETEIGIVEMGANHKKEIEFL
CEIAQPDYGYITNFGKAHLEGFGGVQGVIEGKSEMYQYLLKNNKTVFVNLEDPIQIEKSTGIKNFTFGVNKESADLKIKN
IQANPFVVIEYEDFKVESHLIGLYNANNINAAVSIGKYFNVEDALIKEAIENYIPANNRSQLLKKGSNEIILDAYNANPS
SMAVAITNFLQLENQNKIMILGDMFELGNESHEEHKAIVDSLSNQNKSKCYLIGKSFYENKISNENIQFYETFDAFAAYL
KTIEFKDNTILIKGSRGMALERTLDYIS
>Mature_428_residues
MNIQEIHNLFLKCQSLSIDTRKIEKGSMFFAIKGENFDANTFAKEALDLGALYVVIDNESYFIDDRTILVNNSLETLQEL
SKFHRNYLKLPIVALTGSNGKTTTKELINVVLSKKFKTKATIGNLNNHIGVPLTLLSFTKETEIGIVEMGANHKKEIEFL
CEIAQPDYGYITNFGKAHLEGFGGVQGVIEGKSEMYQYLLKNNKTVFVNLEDPIQIEKSTGIKNFTFGVNKESADLKIKN
IQANPFVVIEYEDFKVESHLIGLYNANNINAAVSIGKYFNVEDALIKEAIENYIPANNRSQLLKKGSNEIILDAYNANPS
SMAVAITNFLQLENQNKIMILGDMFELGNESHEEHKAIVDSLSNQNKSKCYLIGKSFYENKISNENIQFYETFDAFAAYL
KTIEFKDNTILIKGSRGMALERTLDYIS

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=435, Percent_Identity=29.6551724137931, Blast_Score=181, Evalue=8e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48229; Mature: 48229

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQEIHNLFLKCQSLSIDTRKIEKGSMFFAIKGENFDANTFAKEALDLGALYVVIDNES
CCHHHHHHHHHHHHCCCCCHHEECCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCE
YFIDDRTILVNNSLETLQELSKFHRNYLKLPIVALTGSNGKTTTKELINVVLSKKFKTKA
EEEECEEEEECCCHHHHHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCE
TIGNLNNHIGVPLTLLSFTKETEIGIVEMGANHKKEIEFLCEIAQPDYGYITNFGKAHLE
EECCCCCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHCCCCHHHHC
GFGGVQGVIEGKSEMYQYLLKNNKTVFVNLEDPIQIEKSTGIKNFTFGVNKESADLKIKN
CCCCCCEEECCHHHHHHHHHHCCCEEEEEECCCEEEECCCCCEEEEEECCCCCCCEEEEE
IQANPFVVIEYEDFKVESHLIGLYNANNINAAVSIGKYFNVEDALIKEAIENYIPANNRS
ECCCCEEEEEEECEEEEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHH
QLLKKGSNEIILDAYNANPSSMAVAITNFLQLENQNKIMILGDMFELGNESHEEHKAIVD
HHHHCCCCEEEEEEECCCCCHHHHEEEHHHEECCCCEEEEEECHHHCCCCCCHHHHHHHH
SLSNQNKSKCYLIGKSFYENKISNENIQFYETFDAFAAYLKTIEFKDNTILIKGSRGMAL
HHCCCCCCEEEEEECHHHHCCCCCCCEEEEEHHHHHHHHHHEEEECCCEEEEECCCCCCH
ERTLDYIS
HHHHHHCC
>Mature Secondary Structure
MNIQEIHNLFLKCQSLSIDTRKIEKGSMFFAIKGENFDANTFAKEALDLGALYVVIDNES
CCHHHHHHHHHHHHCCCCCHHEECCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCE
YFIDDRTILVNNSLETLQELSKFHRNYLKLPIVALTGSNGKTTTKELINVVLSKKFKTKA
EEEECEEEEECCCHHHHHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCE
TIGNLNNHIGVPLTLLSFTKETEIGIVEMGANHKKEIEFLCEIAQPDYGYITNFGKAHLE
EECCCCCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHCCCCHHHHC
GFGGVQGVIEGKSEMYQYLLKNNKTVFVNLEDPIQIEKSTGIKNFTFGVNKESADLKIKN
CCCCCCEEECCHHHHHHHHHHCCCEEEEEECCCEEEECCCCCEEEEEECCCCCCCEEEEE
IQANPFVVIEYEDFKVESHLIGLYNANNINAAVSIGKYFNVEDALIKEAIENYIPANNRS
ECCCCEEEEEEECEEEEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHH
QLLKKGSNEIILDAYNANPSSMAVAITNFLQLENQNKIMILGDMFELGNESHEEHKAIVD
HHHHCCCCEEEEEEECCCCCHHHHEEEHHHEECCCCEEEEEECHHHCCCCCCHHHHHHHH
SLSNQNKSKCYLIGKSFYENKISNENIQFYETFDAFAAYLKTIEFKDNTILIKGSRGMAL
HHCCCCCCEEEEEECHHHHCCCCCCCEEEEEHHHHHHHHHHEEEECCCEEEEECCCCCCH
ERTLDYIS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]