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The map label for this gene is ansA [H]

Identifier: 146299040

GI number: 146299040

Start: 1463048

End: 1464076

Strand: Direct

Name: ansA [H]

Synonym: Fjoh_1280

Alternate gene names: 146299040

Gene position: 1463048-1464076 (Clockwise)

Preceding gene: 146299039

Following gene: 146299042

Centisome position: 24.0

GC content: 35.57

Gene sequence:

>1029_bases
ATGTCATCTAAAGCAAAAATATTATTGATTTATACCGGAGGAACCATCGGTATGAGCAAAGACTTTGAAACTGGAGCGCT
TAAAGCCTTCAATTTTGGTAAATTAATTCAGAAAATTCCAGAAATTAAACAATTGGACTGCGAAATCGAATCGATTTCAT
TTGAACATCCAATTGATTCTTCGAATATGAATCCTCAAATGTGGACTAAAATTGCCACAATTATTGAGAAAAATTACGCT
TCTTACGACGGATTTGTTGTTCTTCACGGATCAGATACGATGTCGTATTCGGCTTCGGCATTGAGTTTTATGCTCGAGAA
TTTAGCAAAACCAGTTGTATTTACAGGTTCGCAATTGCCAATTGGAGACTTGCGTACCGATGCAAAAGAAAACCTGATTA
CAGCCATTCAGATTGCATCACTTTTAGAAGACGGAAAACCGGTTATTACAGAAGTCTGTTTGTATTTTGAATACAAATTG
TACCGTGGAAACCGAACTTCAAAAGTAAATGCGGAGCATTTTAGAGCTTTTACAGCGCCAAATTATCCGGAATTGGTAGA
ATCTGGAGTTCATCTAAAATTGAACAAACATTTATTTCTTCCTGTAAATAAGGATGCAAAATTAATCGTTCATAAAAACT
TAGACAACCACGTAGCCATTATTAAAATGTTTCCGGGAATGAGCGAAATTGTTTTGGCTTCAATTCTGGCAACTAAAGGT
TTAAGAGGAATTATTCTGGAAACTTACGGTTCAGGAAATGCTCCAACCGAAGATTGGTTTTTAAATTTAATTGAAAAAGC
CATTAAATCCGGAATGCATATCGTAAACGTAACACAATGTTCTGGCGGAAGCGTGAATATGGGACAGTATGAAACCAGTA
CTGCTTTAAAATCTTTAGGTGTTATTTCCGGAAAAGACATTACTACAGAAGCCGCAATTACAAAATTGATGTATTTACTG
GGTCATAATATTCCTCAAAACGAGTTTAAAGATATTTTTGAAACGGCACTTCGCGGGGAAATATCTTAA

Upstream 100 bases:

>100_bases
CGTCGTTTAGAAATGAGAATTGGAAGCGATAATCCTGTTACCAGACAAGGATTTTTGGCTATGTATGCCAATCCTGTTGT
CAATAAATTAAAATACCAAG

Downstream 100 bases:

>100_bases
ATTTAAATTACAATCTATTTTGAAATAAAGAACTTTGACAAAGTTGAAGATTCATCACGACGAGAATATTCCATAGGAAT
ATCTCATTGGTAGAAAAAAA

Product: L-asparaginase, type I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSSKAKILLIYTGGTIGMSKDFETGALKAFNFGKLIQKIPEIKQLDCEIESISFEHPIDSSNMNPQMWTKIATIIEKNYA
SYDGFVVLHGSDTMSYSASALSFMLENLAKPVVFTGSQLPIGDLRTDAKENLITAIQIASLLEDGKPVITEVCLYFEYKL
YRGNRTSKVNAEHFRAFTAPNYPELVESGVHLKLNKHLFLPVNKDAKLIVHKNLDNHVAIIKMFPGMSEIVLASILATKG
LRGIILETYGSGNAPTEDWFLNLIEKAIKSGMHIVNVTQCSGGSVNMGQYETSTALKSLGVISGKDITTEAAITKLMYLL
GHNIPQNEFKDIFETALRGEIS

Sequences:

>Translated_342_residues
MSSKAKILLIYTGGTIGMSKDFETGALKAFNFGKLIQKIPEIKQLDCEIESISFEHPIDSSNMNPQMWTKIATIIEKNYA
SYDGFVVLHGSDTMSYSASALSFMLENLAKPVVFTGSQLPIGDLRTDAKENLITAIQIASLLEDGKPVITEVCLYFEYKL
YRGNRTSKVNAEHFRAFTAPNYPELVESGVHLKLNKHLFLPVNKDAKLIVHKNLDNHVAIIKMFPGMSEIVLASILATKG
LRGIILETYGSGNAPTEDWFLNLIEKAIKSGMHIVNVTQCSGGSVNMGQYETSTALKSLGVISGKDITTEAAITKLMYLL
GHNIPQNEFKDIFETALRGEIS
>Mature_341_residues
SSKAKILLIYTGGTIGMSKDFETGALKAFNFGKLIQKIPEIKQLDCEIESISFEHPIDSSNMNPQMWTKIATIIEKNYAS
YDGFVVLHGSDTMSYSASALSFMLENLAKPVVFTGSQLPIGDLRTDAKENLITAIQIASLLEDGKPVITEVCLYFEYKLY
RGNRTSKVNAEHFRAFTAPNYPELVESGVHLKLNKHLFLPVNKDAKLIVHKNLDNHVAIIKMFPGMSEIVLASILATKGL
RGIILETYGSGNAPTEDWFLNLIEKAIKSGMHIVNVTQCSGGSVNMGQYETSTALKSLGVISGKDITTEAAITKLMYLLG
HNIPQNEFKDIFETALRGEIS

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=361, Percent_Identity=36.8421052631579, Blast_Score=230, Evalue=2e-60,
Organism=Escherichia coli, GI1788065, Length=341, Percent_Identity=41.0557184750733, Blast_Score=266, Evalue=1e-72,
Organism=Escherichia coli, GI1789327, Length=256, Percent_Identity=25.390625, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193207372, Length=369, Percent_Identity=37.6693766937669, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17558280, Length=369, Percent_Identity=37.940379403794, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17558278, Length=369, Percent_Identity=37.940379403794, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI193207374, Length=369, Percent_Identity=37.6693766937669, Blast_Score=232, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6320527, Length=359, Percent_Identity=25.3481894150418, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24639609, Length=372, Percent_Identity=38.1720430107527, Blast_Score=237, Evalue=9e-63,
Organism=Drosophila melanogaster, GI24645530, Length=371, Percent_Identity=38.2749326145553, Blast_Score=232, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37690; Mature: 37559

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKAKILLIYTGGTIGMSKDFETGALKAFNFGKLIQKIPEIKQLDCEIESISFEHPIDS
CCCCCEEEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCC
SNMNPQMWTKIATIIEKNYASYDGFVVLHGSDTMSYSASALSFMLENLAKPVVFTGSQLP
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCC
IGDLRTDAKENLITAIQIASLLEDGKPVITEVCLYFEYKLYRGNRTSKVNAEHFRAFTAP
CCHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCCCCHHHHEEECCC
NYPELVESGVHLKLNKHLFLPVNKDAKLIVHKNLDNHVAIIKMFPGMSEIVLASILATKG
CCHHHHHCCCEEEECCEEEEEECCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHCC
LRGIILETYGSGNAPTEDWFLNLIEKAIKSGMHIVNVTQCSGGSVNMGQYETSTALKSLG
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHCC
VISGKDITTEAAITKLMYLLGHNIPQNEFKDIFETALRGEIS
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSKAKILLIYTGGTIGMSKDFETGALKAFNFGKLIQKIPEIKQLDCEIESISFEHPIDS
CCCCEEEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCC
SNMNPQMWTKIATIIEKNYASYDGFVVLHGSDTMSYSASALSFMLENLAKPVVFTGSQLP
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCC
IGDLRTDAKENLITAIQIASLLEDGKPVITEVCLYFEYKLYRGNRTSKVNAEHFRAFTAP
CCHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCCCCHHHHEEECCC
NYPELVESGVHLKLNKHLFLPVNKDAKLIVHKNLDNHVAIIKMFPGMSEIVLASILATKG
CCHHHHHCCCEEEECCEEEEEECCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHCC
LRGIILETYGSGNAPTEDWFLNLIEKAIKSGMHIVNVTQCSGGSVNMGQYETSTALKSLG
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHCC
VISGKDITTEAAITKLMYLLGHNIPQNEFKDIFETALRGEIS
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]