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The map label for this gene is dut

Identifier: 146298824

GI number: 146298824

Start: 1206718

End: 1207152

Strand: Direct

Name: dut

Synonym: Fjoh_1063

Alternate gene names: 146298824

Gene position: 1206718-1207152 (Clockwise)

Preceding gene: 146298823

Following gene: 146298825

Centisome position: 19.79

GC content: 36.55

Gene sequence:

>435_bases
ATGAAAATACAAATTATCAATAAATCACGACACGATTTACCAAATTACGAAACAATAGCTTCTGCAGGAATGGATCTGCG
TGCCAATATTATAGAACCAATTACGTTAAAACCTTTAGAAAGAACAATTGTAAAAACGGGTCTTTTTATTGAATTGCCAA
TTGGTTACGAAGCTCAGGTAAGACCAAGAAGCGGACTGGCAGCAAAAAAAGGAGTTACCGTTTTAAATTCTCCAGGAACT
GTAGATGCTGATTACAGAGGTGAGATAGGTGTGATTTTGGTGAATTTATCAAATGAAGAATTTGTAATCGAAAACGGAGA
AAGAATTGCACAGTTAATTATTGCCAAACACGAAAGAGCAGAGTGGATTGAGGTTGAAGAACTTTCTGAAACTTCAAGAG
GAGAAGGCGGATTTGGAAGTACCGGCGTAAAATAA

Upstream 100 bases:

>100_bases
TTATTAGCCTTTATGTACTTTGTGTACCACAACGAAAAAGACACGATCAAAGGAATTATGAACAGAAAGTAAAGATGCTT
TTTTAATCTTTTATAATAAA

Downstream 100 bases:

>100_bases
TTTTCAGTCGCAGTTTTCAGTTTTTTACAGGAATTACTAAATGTTGTAGAATTCTCTGCGAATTTTAAAATTAAAACTTG
AACTAACCAAACAGGAGATT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 144; Mature: 144

Protein sequence:

>144_residues
MKIQIINKSRHDLPNYETIASAGMDLRANIIEPITLKPLERTIVKTGLFIELPIGYEAQVRPRSGLAAKKGVTVLNSPGT
VDADYRGEIGVILVNLSNEEFVIENGERIAQLIIAKHERAEWIEVEELSETSRGEGGFGSTGVK

Sequences:

>Translated_144_residues
MKIQIINKSRHDLPNYETIASAGMDLRANIIEPITLKPLERTIVKTGLFIELPIGYEAQVRPRSGLAAKKGVTVLNSPGT
VDADYRGEIGVILVNLSNEEFVIENGERIAQLIIAKHERAEWIEVEELSETSRGEGGFGSTGVK
>Mature_144_residues
MKIQIINKSRHDLPNYETIASAGMDLRANIIEPITLKPLERTIVKTGLFIELPIGYEAQVRPRSGLAAKKGVTVLNSPGT
VDADYRGEIGVILVNLSNEEFVIENGERIAQLIIAKHERAEWIEVEELSETSRGEGGFGSTGVK

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=143, Percent_Identity=42.6573426573427, Blast_Score=107, Evalue=4e-24,
Organism=Homo sapiens, GI4503423, Length=143, Percent_Identity=42.6573426573427, Blast_Score=106, Evalue=6e-24,
Organism=Homo sapiens, GI70906441, Length=143, Percent_Identity=42.6573426573427, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1790071, Length=131, Percent_Identity=42.7480916030534, Blast_Score=105, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71988561, Length=129, Percent_Identity=46.5116279069767, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319729, Length=143, Percent_Identity=39.1608391608392, Blast_Score=100, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19921126, Length=126, Percent_Identity=44.4444444444444, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24583610, Length=126, Percent_Identity=44.4444444444444, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_FLAJ1 (A5FL22)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001193415.1
- ProteinModelPortal:   A5FL22
- SMR:   A5FL22
- STRING:   A5FL22
- GeneID:   5091793
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_1063
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   HGIALVN
- ProtClustDB:   PRK00601
- BioCyc:   FJOH376686:FJOH_1063-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15812; Mature: 15812

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: BINDING 76-76

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIQIINKSRHDLPNYETIASAGMDLRANIIEPITLKPLERTIVKTGLFIELPIGYEAQV
CEEEEECCCCCCCCCHHHHHHCCCCEECCCCCCEEECHHHHHHHHCCCEEEECCCCCEEE
RPRSGLAAKKGVTVLNSPGTVDADYRGEIGVILVNLSNEEFVIENGERIAQLIIAKHERA
CCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEEECCCEEEEECCHHHHHHHHHCCCCC
EWIEVEELSETSRGEGGFGSTGVK
CEEEHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIQIINKSRHDLPNYETIASAGMDLRANIIEPITLKPLERTIVKTGLFIELPIGYEAQV
CEEEEECCCCCCCCCHHHHHHCCCCEECCCCCCEEECHHHHHHHHCCCEEEECCCCCEEE
RPRSGLAAKKGVTVLNSPGTVDADYRGEIGVILVNLSNEEFVIENGERIAQLIIAKHERA
CCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEEECCCEEEEECCHHHHHHHHHCCCCC
EWIEVEELSETSRGEGGFGSTGVK
CEEEHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA