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The map label for this gene is fumB [H]

Identifier: 146298643

GI number: 146298643

Start: 994028

End: 995632

Strand: Direct

Name: fumB [H]

Synonym: Fjoh_0881

Alternate gene names: 146298643

Gene position: 994028-995632 (Clockwise)

Preceding gene: 146298642

Following gene: 146298646

Centisome position: 16.3

GC content: 38.88

Gene sequence:

>1605_bases
ATGATTGATTTTATATACCAAGATCCTTATCCAATTTTAAAGGATGATACGCAGTACCGCAAAATCACCTCTGATTTTGT
AAAAGTTGAAAAATTTGGAGAACGTGAAGTTTTAACCGTTGATCCAAAAGGTTTAGAATTATTAGCTGAAGAAGCTTTAA
CCGATGTTTCGTTCATGCTTAGAACGTCGCATTTACAAAAACTAAGAAACATTCTTGACGACCCAGAGGCTACAGATAAC
GATCGTTTTGTTGCCTACAATTTACTTCAAAATGCTTCAGTTGCTGCCGAAGGTCAATTACCAAGCTGTCAGGATACCGG
AACAGCAATTGTAATGGCTAAAAAAGGCGAAAGCATCTTTACAGGTGTTGATGATGCTGAATGGTTAAGCAAAGGAATTT
TCAATACATACCAAAAACGTAATTTACGTTATTCGCAGATTGTTCCGATTTCGATGTTTGAAGAGAAAAACTCAGGATCG
AATCTTCCGGCACAAATTGATATTTATGCAAAAAAAGGAACTTCTTACGAATTCCTTTTTATGGCAAAAGGAGGCGGATC
TGCAAATAAAACATACTTATACCAACAGACAAAATCGTTATTGAACGATAAATCTATGGATGCATTTATCCGCGCCAAAA
TCAAAGACTTAGGAACTTCGGCTTGTCCGCCTTATCATTTGGCTTTAGTAATTGGAGGAACTTCTGCGGAAGCGAACCTA
AGTGCTGTTAAAAAAGCTTCTGCCGGATATTATGACCATCTGCCAACTTCAGGAAACATGGCTGGTCAGGCATTTCGTGA
TTTGGAATGGGAAGAACGCGTTCAGAAAATCTGTCAGGAAAGTGAAATTGGAGCTCAGTTTGGAGGAAAATATTTCACGC
ACGACGTTCGTGTCATTCGTTTACCTCGTCACGCTGCTTCTTGTCCGGTTGGATTAGGGGTTTCTTGCTCTGCAGATAGA
AACATCAAAGGAAAAATTACAAAAGACGGAATCTTCGTTGAACAGTTAGAAGTAAATCCAAAACAGTTTTTACCAGAAAC
GGCGCCACATTTAGAAGCTCCGGTTGAAGTTGACTTAGATCAGCCAATGGCGGATATTTTGGCTAAACTATCTCAATATC
CTATCAAAACCCGTTTAAAACTAAACGGAACTGTAATTGTAGCCAGAGATATTGCTCACGCAAAAATTAAAGAATTATTA
GACGCCGGAAAACCAATGCCGGAATACTTTAAAAACCATCCGGTATATTATGCTGGTCCTGCAAAAACTCCGGAAGGAAT
GGCTTCAGGTAGTTTCGGCCCTACAACAGCCGGACGTATGGACGTTTATGTAGATGAATTCCAGAAAAATGGCGGAAGCA
TGGTTATGCTTGCTAAAGGAAATCGTACGAAACAAGTTACTGATGCCTGCAACAAATATGGCGGATTCTATCTAGGTTCT
ATTGGAGGTCCTGCTGCTATTTTGGCACAAGATAATATCTTAAAAGTAGAAGTGGTTGATTTTGAAGAATTAGGAATGGA
AGCAGTCCGTAAAATCACTGTTAAAGACTTCCCTGCCTTCATTATCACGGATGATAAAGGGAATGATTTCTTTGAAAATT
TATAA

Upstream 100 bases:

>100_bases
CTTTTAACGAGTTTCACAACCAGCAATAAACTGTTTTAAGAGGTCTTATTCAAATTCTTTCTTAGGTAAATAATTTCTAA
CTTTGTAAAAAAACAAAGAA

Downstream 100 bases:

>100_bases
TTTTTTTTAGTTGCTAAGGTACTGAGGTTCTAAGCTGCTAAGCTTTAAATACAAAACCGCCTGTTTGGGCGGTTTTTTGT
TTCTATTAACTTAGTGCCTG

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase subunit beta

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MIDFIYQDPYPILKDDTQYRKITSDFVKVEKFGEREVLTVDPKGLELLAEEALTDVSFMLRTSHLQKLRNILDDPEATDN
DRFVAYNLLQNASVAAEGQLPSCQDTGTAIVMAKKGESIFTGVDDAEWLSKGIFNTYQKRNLRYSQIVPISMFEEKNSGS
NLPAQIDIYAKKGTSYEFLFMAKGGGSANKTYLYQQTKSLLNDKSMDAFIRAKIKDLGTSACPPYHLALVIGGTSAEANL
SAVKKASAGYYDHLPTSGNMAGQAFRDLEWEERVQKICQESEIGAQFGGKYFTHDVRVIRLPRHAASCPVGLGVSCSADR
NIKGKITKDGIFVEQLEVNPKQFLPETAPHLEAPVEVDLDQPMADILAKLSQYPIKTRLKLNGTVIVARDIAHAKIKELL
DAGKPMPEYFKNHPVYYAGPAKTPEGMASGSFGPTTAGRMDVYVDEFQKNGGSMVMLAKGNRTKQVTDACNKYGGFYLGS
IGGPAAILAQDNILKVEVVDFEELGMEAVRKITVKDFPAFIITDDKGNDFFENL

Sequences:

>Translated_534_residues
MIDFIYQDPYPILKDDTQYRKITSDFVKVEKFGEREVLTVDPKGLELLAEEALTDVSFMLRTSHLQKLRNILDDPEATDN
DRFVAYNLLQNASVAAEGQLPSCQDTGTAIVMAKKGESIFTGVDDAEWLSKGIFNTYQKRNLRYSQIVPISMFEEKNSGS
NLPAQIDIYAKKGTSYEFLFMAKGGGSANKTYLYQQTKSLLNDKSMDAFIRAKIKDLGTSACPPYHLALVIGGTSAEANL
SAVKKASAGYYDHLPTSGNMAGQAFRDLEWEERVQKICQESEIGAQFGGKYFTHDVRVIRLPRHAASCPVGLGVSCSADR
NIKGKITKDGIFVEQLEVNPKQFLPETAPHLEAPVEVDLDQPMADILAKLSQYPIKTRLKLNGTVIVARDIAHAKIKELL
DAGKPMPEYFKNHPVYYAGPAKTPEGMASGSFGPTTAGRMDVYVDEFQKNGGSMVMLAKGNRTKQVTDACNKYGGFYLGS
IGGPAAILAQDNILKVEVVDFEELGMEAVRKITVKDFPAFIITDDKGNDFFENL
>Mature_534_residues
MIDFIYQDPYPILKDDTQYRKITSDFVKVEKFGEREVLTVDPKGLELLAEEALTDVSFMLRTSHLQKLRNILDDPEATDN
DRFVAYNLLQNASVAAEGQLPSCQDTGTAIVMAKKGESIFTGVDDAEWLSKGIFNTYQKRNLRYSQIVPISMFEEKNSGS
NLPAQIDIYAKKGTSYEFLFMAKGGGSANKTYLYQQTKSLLNDKSMDAFIRAKIKDLGTSACPPYHLALVIGGTSAEANL
SAVKKASAGYYDHLPTSGNMAGQAFRDLEWEERVQKICQESEIGAQFGGKYFTHDVRVIRLPRHAASCPVGLGVSCSADR
NIKGKITKDGIFVEQLEVNPKQFLPETAPHLEAPVEVDLDQPMADILAKLSQYPIKTRLKLNGTVIVARDIAHAKIKELL
DAGKPMPEYFKNHPVYYAGPAKTPEGMASGSFGPTTAGRMDVYVDEFQKNGGSMVMLAKGNRTKQVTDACNKYGGFYLGS
IGGPAAILAQDNILKVEVVDFEELGMEAVRKITVKDFPAFIITDDKGNDFFENL

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=534, Percent_Identity=61.9850187265918, Blast_Score=707, Evalue=0.0,
Organism=Escherichia coli, GI1787897, Length=534, Percent_Identity=61.7977528089888, Blast_Score=703, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=155, Percent_Identity=29.6774193548387, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 58938; Mature: 58938

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDFIYQDPYPILKDDTQYRKITSDFVKVEKFGEREVLTVDPKGLELLAEEALTDVSFML
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHH
RTSHLQKLRNILDDPEATDNDRFVAYNLLQNASVAAEGQLPSCQDTGTAIVMAKKGESIF
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCCEEE
TGVDDAEWLSKGIFNTYQKRNLRYSQIVPISMFEEKNSGSNLPAQIDIYAKKGTSYEFLF
ECCCCHHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCCCCCEEEEEEEECCCCEEEEE
MAKGGGSANKTYLYQQTKSLLNDKSMDAFIRAKIKDLGTSACPPYHLALVIGGTSAEANL
EECCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCHH
SAVKKASAGYYDHLPTSGNMAGQAFRDLEWEERVQKICQESEIGAQFGGKYFTHDVRVIR
HHHHHHCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCHHHCCEEEECCEEEEE
LPRHAASCPVGLGVSCSADRNIKGKITKDGIFVEQLEVNPKQFLPETAPHLEAPVEVDLD
ECCCCCCCCCCCCEECCCCCCCCCEEECCCEEEEEEECCHHHHCCCCCCCCCCCEEECCC
QPMADILAKLSQYPIKTRLKLNGTVIVARDIAHAKIKELLDAGKPMPEYFKNHPVYYAGP
CCHHHHHHHHHCCCCEEEEEECCEEEEEECHHHHHHHHHHHCCCCCHHHHHCCCEEEECC
AKTPEGMASGSFGPTTAGRMDVYVDEFQKNGGSMVMLAKGNRTKQVTDACNKYGGFYLGS
CCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEC
IGGPAAILAQDNILKVEVVDFEELGMEAVRKITVKDFPAFIITDDKGNDFFENL
CCCCEEEEECCCEEEEEEECHHHHCHHHHHHHHHCCCCEEEEECCCCCHHHHCC
>Mature Secondary Structure
MIDFIYQDPYPILKDDTQYRKITSDFVKVEKFGEREVLTVDPKGLELLAEEALTDVSFML
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHH
RTSHLQKLRNILDDPEATDNDRFVAYNLLQNASVAAEGQLPSCQDTGTAIVMAKKGESIF
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCCEEE
TGVDDAEWLSKGIFNTYQKRNLRYSQIVPISMFEEKNSGSNLPAQIDIYAKKGTSYEFLF
ECCCCHHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCCCCCEEEEEEEECCCCEEEEE
MAKGGGSANKTYLYQQTKSLLNDKSMDAFIRAKIKDLGTSACPPYHLALVIGGTSAEANL
EECCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCHH
SAVKKASAGYYDHLPTSGNMAGQAFRDLEWEERVQKICQESEIGAQFGGKYFTHDVRVIR
HHHHHHCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCHHHCCEEEECCEEEEE
LPRHAASCPVGLGVSCSADRNIKGKITKDGIFVEQLEVNPKQFLPETAPHLEAPVEVDLD
ECCCCCCCCCCCCEECCCCCCCCCEEECCCEEEEEEECCHHHHCCCCCCCCCCCEEECCC
QPMADILAKLSQYPIKTRLKLNGTVIVARDIAHAKIKELLDAGKPMPEYFKNHPVYYAGP
CCHHHHHHHHHCCCCEEEEEECCEEEEEECHHHHHHHHHHHCCCCCHHHHHCCCEEEECC
AKTPEGMASGSFGPTTAGRMDVYVDEFQKNGGSMVMLAKGNRTKQVTDACNKYGGFYLGS
CCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEC
IGGPAAILAQDNILKVEVVDFEELGMEAVRKITVKDFPAFIITDDKGNDFFENL
CCCCEEEEECCCEEEEEEECHHHHCHHHHHHHHHCCCCEEEEECCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]