Click here to switch to the map view.

The map label for this gene is ogt [H]

Identifier: 146298638

GI number: 146298638

Start: 988732

End: 989208

Strand: Direct

Name: ogt [H]

Synonym: Fjoh_0876

Alternate gene names: 146298638

Gene position: 988732-989208 (Clockwise)

Preceding gene: 146298637

Following gene: 146298639

Centisome position: 16.22

GC content: 36.9

Gene sequence:

>477_bases
ATGGAAACAGTATATATCAATTCGCCTTTAGGAATTACCAAAATAGTTGGTGATGAAGCAGGTATTGCAGCAATCTCTGT
TTCTGATGTAGGAACAAACGAAGTTTCAAAAAAAATTCCAAAAGTTTTAAAAGATGCAGTTTCGCAGCTTGAAGAATACT
TTGAAGGCAAAAGAACCAATTTTGACTTAAAACTAAATCCTCAGGGTACCGAATTTCAACAAAAAGTCTGGAAATCATTA
CTAGAAATTCCATACGGAAAAACGGTAAGTTATATGGATCAAACCAAAAAGCTGGGTGATATAAAAGCAATTCGGGCCGT
GGCTTCAGCAAACGGAAAAAATCCGCTTTGGATTGTAGTTCCCTGTCATCGTGTTATTGGCACAAATGGTTCTTTAACAG
GTTACGCCGGCGGATTATCACGCAAAAAATGGCTTTTGGAACACGAAAGCCCAAGTAAACAACAAAGTTTGTTTTAA

Upstream 100 bases:

>100_bases
CATAGCAAAACAATTACTGATCTAACTTATATGGTTTAAAAAACTTTGCGAACTTTGCGAAACCATCGTGTCTATGCGGT
AAAAACTTTACGGTTAAATT

Downstream 100 bases:

>100_bases
GAATATCAGCCGCGAATTATTTCTATTCTCTTCAATTTTGATTTTTCTGGAATAACTGATTTTCTTACAATAAGAAAATT
CATTCTTTTTTATAAAACTC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
METVYINSPLGITKIVGDEAGIAAISVSDVGTNEVSKKIPKVLKDAVSQLEEYFEGKRTNFDLKLNPQGTEFQQKVWKSL
LEIPYGKTVSYMDQTKKLGDIKAIRAVASANGKNPLWIVVPCHRVIGTNGSLTGYAGGLSRKKWLLEHESPSKQQSLF

Sequences:

>Translated_158_residues
METVYINSPLGITKIVGDEAGIAAISVSDVGTNEVSKKIPKVLKDAVSQLEEYFEGKRTNFDLKLNPQGTEFQQKVWKSL
LEIPYGKTVSYMDQTKKLGDIKAIRAVASANGKNPLWIVVPCHRVIGTNGSLTGYAGGLSRKKWLLEHESPSKQQSLF
>Mature_158_residues
METVYINSPLGITKIVGDEAGIAAISVSDVGTNEVSKKIPKVLKDAVSQLEEYFEGKRTNFDLKLNPQGTEFQQKVWKSL
LEIPYGKTVSYMDQTKKLGDIKAIRAVASANGKNPLWIVVPCHRVIGTNGSLTGYAGGLSRKKWLLEHESPSKQQSLF

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=166, Percent_Identity=31.9277108433735, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1787596, Length=120, Percent_Identity=50, Blast_Score=115, Evalue=1e-27,
Organism=Escherichia coli, GI1788542, Length=160, Percent_Identity=35.625, Blast_Score=101, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=46.25, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=46.25, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=89, Percent_Identity=44.9438202247191, Blast_Score=81, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17380; Mature: 17380

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METVYINSPLGITKIVGDEAGIAAISVSDVGTNEVSKKIPKVLKDAVSQLEEYFEGKRTN
CCEEEECCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FDLKLNPQGTEFQQKVWKSLLEIPYGKTVSYMDQTKKLGDIKAIRAVASANGKNPLWIVV
CEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
PCHRVIGTNGSLTGYAGGLSRKKWLLEHESPSKQQSLF
ECHHHHCCCCCCCCCCCCCCCHHEEECCCCCCHHCCCC
>Mature Secondary Structure
METVYINSPLGITKIVGDEAGIAAISVSDVGTNEVSKKIPKVLKDAVSQLEEYFEGKRTN
CCEEEECCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FDLKLNPQGTEFQQKVWKSLLEIPYGKTVSYMDQTKKLGDIKAIRAVASANGKNPLWIVV
CEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
PCHRVIGTNGSLTGYAGGLSRKKWLLEHESPSKQQSLF
ECHHHHCCCCCCCCCCCCCCCHHEEECCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA