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The map label for this gene is kdgA [H]

Identifier: 146298433

GI number: 146298433

Start: 733576

End: 734223

Strand: Direct

Name: kdgA [H]

Synonym: Fjoh_0670

Alternate gene names: 146298433

Gene position: 733576-734223 (Clockwise)

Preceding gene: 146298432

Following gene: 146298434

Centisome position: 12.03

GC content: 36.88

Gene sequence:

>648_bases
ATGTACAGCATTTTAAAAACGCAAGGTGTACTTCCGTTAGTAACCCAAATCAATATCCAAACAGCCCAAATCGTATTGCA
ATCAGCGGCTGATGCTGGCATAAAAATTATCGAGTTCGCAGCTCGTGCAGATGATGCAAAAGAAGTTTTTGCTCAAATGA
CAGCTTTTAAAAAAGAAAACAATTTAGATGTAAAAATAGCAGTGGGATCGATTTTAAGTGTGGCCGATGCCGAAACTTAT
CATCAATTGGGAGCAGATTGTATTATCTGTCCGCACACTGATCTGGAAATTGGCAATTACTGTTTTAAAAACAATATTTA
CTGGATTCCGGGCGCTGCAACCTTAAATGAAATTCTTCATGCCAACAAACTGGGTGCCGAAGTTGTAAAACTTTTTCCGG
CTGATAAAATTGGCGGACCGGGATATGTAAAAGCAATAAGAGCGCCTTTCCCAAATTTAAAAATAATGCCCACAGGAGGC
GTAACCCTTGAAGAAAGCAATTTAAAATCGTGGTTTAAATCTGGAGTGGTATGCGTTGGAATTGGTTCAAATTTATTTTC
AAAAGAAATGCTTTTGAATTTAAATTATGAAGAAGCGCTCCAGGCTTTTCAAAATTTAATTGAAGTAGTAGAAAAAACAA
GAAACTAA

Upstream 100 bases:

>100_bases
ACAAAGTATACACGGAGATTTTGCCATAAGCACGCTCGACGAAATACGTCATTTTATTAAAAATGGTTCAAGCAATAGAA
TTAACAGATAAAAAAAGAAT

Downstream 100 bases:

>100_bases
GTTATGGAAGAAAATTGGTGGAAAATTAAATCTGATATCTTAATTGATACGCCGTTTTTAGCTGTTTACGAAGATCGTAT
CCGGTTTAATCTGGAGCGTC

Product: KDPG and KHG aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MYSILKTQGVLPLVTQINIQTAQIVLQSAADAGIKIIEFAARADDAKEVFAQMTAFKKENNLDVKIAVGSILSVADAETY
HQLGADCIICPHTDLEIGNYCFKNNIYWIPGAATLNEILHANKLGAEVVKLFPADKIGGPGYVKAIRAPFPNLKIMPTGG
VTLEESNLKSWFKSGVVCVGIGSNLFSKEMLLNLNYEEALQAFQNLIEVVEKTRN

Sequences:

>Translated_215_residues
MYSILKTQGVLPLVTQINIQTAQIVLQSAADAGIKIIEFAARADDAKEVFAQMTAFKKENNLDVKIAVGSILSVADAETY
HQLGADCIICPHTDLEIGNYCFKNNIYWIPGAATLNEILHANKLGAEVVKLFPADKIGGPGYVKAIRAPFPNLKIMPTGG
VTLEESNLKSWFKSGVVCVGIGSNLFSKEMLLNLNYEEALQAFQNLIEVVEKTRN
>Mature_215_residues
MYSILKTQGVLPLVTQINIQTAQIVLQSAADAGIKIIEFAARADDAKEVFAQMTAFKKENNLDVKIAVGSILSVADAETY
HQLGADCIICPHTDLEIGNYCFKNNIYWIPGAATLNEILHANKLGAEVVKLFPADKIGGPGYVKAIRAPFPNLKIMPTGG
VTLEESNLKSWFKSGVVCVGIGSNLFSKEMLLNLNYEEALQAFQNLIEVVEKTRN

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI48994954, Length=147, Percent_Identity=27.891156462585, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 23443; Mature: 23443

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSILKTQGVLPLVTQINIQTAQIVLQSAADAGIKIIEFAARADDAKEVFAQMTAFKKEN
CCCCHHHCCCCEEHEEECHHHHHHHHHHHHCCCEEEEHHHHCCCHHHHHHHHHHHHHCCC
NLDVKIAVGSILSVADAETYHQLGADCIICPHTDLEIGNYCFKNNIYWIPGAATLNEILH
CCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEECCCEEECCHHHHHHHHH
ANKLGAEVVKLFPADKIGGPGYVKAIRAPFPNLKIMPTGGVTLEESNLKSWFKSGVVCVG
HHHHHHHHHHHCCCCCCCCCHHHHHEECCCCCCEEEECCCCEEEHHHHHHHHHCCEEEEE
IGSNLFSKEMLLNLNYEEALQAFQNLIEVVEKTRN
ECCCHHHHHHHEECCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYSILKTQGVLPLVTQINIQTAQIVLQSAADAGIKIIEFAARADDAKEVFAQMTAFKKEN
CCCCHHHCCCCEEHEEECHHHHHHHHHHHHCCCEEEEHHHHCCCHHHHHHHHHHHHHCCC
NLDVKIAVGSILSVADAETYHQLGADCIICPHTDLEIGNYCFKNNIYWIPGAATLNEILH
CCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEECCCEEECCHHHHHHHHH
ANKLGAEVVKLFPADKIGGPGYVKAIRAPFPNLKIMPTGGVTLEESNLKSWFKSGVVCVG
HHHHHHHHHHHCCCCCCCCCHHHHHEECCCCCCEEEECCCCEEEHHHHHHHHHCCEEEEE
IGSNLFSKEMLLNLNYEEALQAFQNLIEVVEKTRN
ECCCHHHHHHHEECCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]