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The map label for this gene is artJ [H]

Identifier: 146284415

GI number: 146284415

Start: 4458457

End: 4459212

Strand: Direct

Name: artJ [H]

Synonym: PST_4104

Alternate gene names: 146284415

Gene position: 4458457-4459212 (Clockwise)

Preceding gene: 146284414

Following gene: 146284416

Centisome position: 97.61

GC content: 58.86

Gene sequence:

>756_bases
ATGAAGAGCTATAAGAAGATCCTGCTGACCGCTGCTGCCTCGCTGATCATCGGCACCCAGGCATTCGCGGCGGAAAAACT
ACGGATTGGTACCGAAGGCGCCTACCCACCCTTCAACCTGATCGACGCCAGCGGCAAGGTGGTCGGCTTCGATCTGGATA
TCTCCCACGCGCTGTGCGCCAAGATGAAGGTCGAGTGCGAGGTGGTTACCTCTGATTGGGACGGCATCATTCCGGCGCTG
AACGCCGGCAAGTTCGACTTCCTCGCCGCCTCCATGTCGGTCACCGAAGAGCGCAAGAACGCCGTCGACTTCACCGACCA
CTACTACACCAACAAGCTGCAGTTCGTGGCGCCGAAGAACGTCGACTTCAAGACCGACGAGAGCTACCTGAAAGGCAAAG
TGATCGGTGCGCAGCGCGCGACCATCGCCGGCACCTGGCTGGAAGACAACCTGGATCGCGTCGTCGACATCAAGCTCTAC
GACACCCAGGAAAACGCCTATCTGGACCTGGCCTCCGGTCGCGTCGACGGCATCCTGGCTGACACCTTCGTGCAGTGGGA
ATGGCTCAAGAGCGATGCCGGCAAGAACTTCGAGTTCAAGGGCGAGCCGGTATTCGATGACGACAAGATCGCCATCGCCG
TGCGCAAGGGCAACGATGAACTGCGCGAGCGGCTGAACAAGGCATTGGCGGAGATCATCGCTGACGGCACCTACAAGCAG
ATCAACGACAAGTACTTCCCGTTCAGCATTTACTGA

Upstream 100 bases:

>100_bases
TGGTCGAGGAGCAGGGAACGCCCGAGCAGGTATTTGACAATCCTCAATCGGCGCGCTGTAAACAATTCATGTCCAGCCAT
CGCTAATGGAGCAACACCGC

Downstream 100 bases:

>100_bases
TCCAACCGAGCCGAAGCCCGGTCGCCAGCCTTCTGGCCGACCGGGCTTCGGTGTTTGAGCTTCTATGATTCCTGACCTTC
ACGGATTCGGTCCGGCCCTG

Product: periplasmic binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKSYKKILLTAAASLIIGTQAFAAEKLRIGTEGAYPPFNLIDASGKVVGFDLDISHALCAKMKVECEVVTSDWDGIIPAL
NAGKFDFLAASMSVTEERKNAVDFTDHYYTNKLQFVAPKNVDFKTDESYLKGKVIGAQRATIAGTWLEDNLDRVVDIKLY
DTQENAYLDLASGRVDGILADTFVQWEWLKSDAGKNFEFKGEPVFDDDKIAIAVRKGNDELRERLNKALAEIIADGTYKQ
INDKYFPFSIY

Sequences:

>Translated_251_residues
MKSYKKILLTAAASLIIGTQAFAAEKLRIGTEGAYPPFNLIDASGKVVGFDLDISHALCAKMKVECEVVTSDWDGIIPAL
NAGKFDFLAASMSVTEERKNAVDFTDHYYTNKLQFVAPKNVDFKTDESYLKGKVIGAQRATIAGTWLEDNLDRVVDIKLY
DTQENAYLDLASGRVDGILADTFVQWEWLKSDAGKNFEFKGEPVFDDDKIAIAVRKGNDELRERLNKALAEIIADGTYKQ
INDKYFPFSIY
>Mature_251_residues
MKSYKKILLTAAASLIIGTQAFAAEKLRIGTEGAYPPFNLIDASGKVVGFDLDISHALCAKMKVECEVVTSDWDGIIPAL
NAGKFDFLAASMSVTEERKNAVDFTDHYYTNKLQFVAPKNVDFKTDESYLKGKVIGAQRATIAGTWLEDNLDRVVDIKLY
DTQENAYLDLASGRVDGILADTFVQWEWLKSDAGKNFEFKGEPVFDDDKIAIAVRKGNDELRERLNKALAEIIADGTYKQ
INDKYFPFSIY

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=246, Percent_Identity=41.0569105691057, Blast_Score=175, Evalue=2e-45,
Organism=Escherichia coli, GI1787088, Length=248, Percent_Identity=39.1129032258064, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=37.1794871794872, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1788649, Length=263, Percent_Identity=37.6425855513308, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1787031, Length=248, Percent_Identity=34.2741935483871, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1788228, Length=222, Percent_Identity=31.981981981982, Blast_Score=124, Evalue=7e-30,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28007; Mature: 28007

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSYKKILLTAAASLIIGTQAFAAEKLRIGTEGAYPPFNLIDASGKVVGFDLDISHALCA
CCHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCCCCCEEEECCCCEEEEECCCHHHHHH
KMKVECEVVTSDWDGIIPALNAGKFDFLAASMSVTEERKNAVDFTDHYYTNKLQFVAPKN
CEEEEEEEEECCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCCEECCEEEEEECCC
VDFKTDESYLKGKVIGAQRATIAGTWLEDNLDRVVDIKLYDTQENAYLDLASGRVDGILA
CCCCCCCHHHCEEEECCCCEEEEEEHHHCCCCEEEEEEEEECCCCEEEEECCCCCCCEEE
DTFVQWEWLKSDAGKNFEFKGEPVFDDDKIAIAVRKGNDELRERLNKALAEIIADGTYKQ
HHHEEEEEECCCCCCCCEECCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHH
INDKYFPFSIY
CCCCCCCEEEC
>Mature Secondary Structure
MKSYKKILLTAAASLIIGTQAFAAEKLRIGTEGAYPPFNLIDASGKVVGFDLDISHALCA
CCHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCCCCCEEEECCCCEEEEECCCHHHHHH
KMKVECEVVTSDWDGIIPALNAGKFDFLAASMSVTEERKNAVDFTDHYYTNKLQFVAPKN
CEEEEEEEEECCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCCEECCEEEEEECCC
VDFKTDESYLKGKVIGAQRATIAGTWLEDNLDRVVDIKLYDTQENAYLDLASGRVDGILA
CCCCCCCHHHCEEEECCCCEEEEEEHHHCCCCEEEEEEEEECCCCEEEEECCCCCCCEEE
DTFVQWEWLKSDAGKNFEFKGEPVFDDDKIAIAVRKGNDELRERLNKALAEIIADGTYKQ
HHHEEEEEECCCCCCCCEECCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHH
INDKYFPFSIY
CCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]