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The map label for this gene is rsmD [H]

Identifier: 146284307

GI number: 146284307

Start: 4333734

End: 4334351

Strand: Direct

Name: rsmD [H]

Synonym: PST_3995

Alternate gene names: 146284307

Gene position: 4333734-4334351 (Clockwise)

Preceding gene: 146284306

Following gene: 146284309

Centisome position: 94.88

GC content: 68.77

Gene sequence:

>618_bases
ATGGCCAATCCACCCATTCGCCCCAGCGCCCATGGCGGCCAGGGCCAGTTGCGCATCATCGGCGGCGAATGGCGCTCGCG
CCGCTTCAGCTTCCCAGATGCCGAAGGCCTGCGGCCGACGCCGGACCGGGTCCGCGAAACCCTGTTCAACTGGCTGGCGC
CCTACCTTTCGGGCGCGCGGGTGCTCGACCCGTTCACCGGCAGCGGCGCGCTCTATCTGGAGGCGCTGTCGCGTGGCGCC
AGCATGGCCCTGGCGCTGGACGTGAACGCCAACGCCATCGCCAGCCTGCGCAAGCACCTGGACACTCTCAACTGCGGCCA
CGGCCAGCTGCTGCAGAGCGACGCCCTGCGCTATCTGGACAGCCAGACGCCGGCGCCCTTCGATCTGGTCTTTCTCGACC
CGCCGTTCAACAGGAACCTGCTGCAGCCGGCCTGCGAGCTGCTCGAAGGCAAGGGTTGGCTGGCCGCGCACGCCTGGGTC
TACACCGAAAGCGAAGCGCCGCCTTCCAGCCTCGGCCTGCCCGGCAACTGGCGACTGCATCGGGAGAAGAAGGCCGGCAA
GGTGTTCTATGCCCTGTGGGAACGCCAGGGCCCCAGCGAGCCAACCGCCACTCAGTAG

Upstream 100 bases:

>100_bases
CCTTCGTCATCGTCACAGCCGGGCCCAGCGTCGAGCAGCAACCGCTGCCGCCGCCCAGCGAAAAACCAATCGAACAGCCC
AGCGGCGTTCCGGAGCATTG

Downstream 100 bases:

>100_bases
AAGTCGACGGTCTGCCCGTGGCGATCGAAGGCGAGAAAGCGCCCGATATTGCCGGTCTCGATCGAGCCGACCATCATCTT
CAACGGCTGCTCGGCCTCGT

Product: methyltransferase, putative

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MANPPIRPSAHGGQGQLRIIGGEWRSRRFSFPDAEGLRPTPDRVRETLFNWLAPYLSGARVLDPFTGSGALYLEALSRGA
SMALALDVNANAIASLRKHLDTLNCGHGQLLQSDALRYLDSQTPAPFDLVFLDPPFNRNLLQPACELLEGKGWLAAHAWV
YTESEAPPSSLGLPGNWRLHREKKAGKVFYALWERQGPSEPTATQ

Sequences:

>Translated_205_residues
MANPPIRPSAHGGQGQLRIIGGEWRSRRFSFPDAEGLRPTPDRVRETLFNWLAPYLSGARVLDPFTGSGALYLEALSRGA
SMALALDVNANAIASLRKHLDTLNCGHGQLLQSDALRYLDSQTPAPFDLVFLDPPFNRNLLQPACELLEGKGWLAAHAWV
YTESEAPPSSLGLPGNWRLHREKKAGKVFYALWERQGPSEPTATQ
>Mature_204_residues
ANPPIRPSAHGGQGQLRIIGGEWRSRRFSFPDAEGLRPTPDRVRETLFNWLAPYLSGARVLDPFTGSGALYLEALSRGAS
MALALDVNANAIASLRKHLDTLNCGHGQLLQSDALRYLDSQTPAPFDLVFLDPPFNRNLLQPACELLEGKGWLAAHAWVY
TESEAPPSSLGLPGNWRLHREKKAGKVFYALWERQGPSEPTATQ

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=193, Percent_Identity=50.259067357513, Blast_Score=192, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22584; Mature: 22453

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANPPIRPSAHGGQGQLRIIGGEWRSRRFSFPDAEGLRPTPDRVRETLFNWLAPYLSGAR
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCE
VLDPFTGSGALYLEALSRGASMALALDVNANAIASLRKHLDTLNCGHGQLLQSDALRYLD
EECCCCCCCHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
SQTPAPFDLVFLDPPFNRNLLQPACELLEGKGWLAAHAWVYTESEAPPSSLGLPGNWRLH
CCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEE
REKKAGKVFYALWERQGPSEPTATQ
CCCCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
ANPPIRPSAHGGQGQLRIIGGEWRSRRFSFPDAEGLRPTPDRVRETLFNWLAPYLSGAR
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCE
VLDPFTGSGALYLEALSRGASMALALDVNANAIASLRKHLDTLNCGHGQLLQSDALRYLD
EECCCCCCCHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
SQTPAPFDLVFLDPPFNRNLLQPACELLEGKGWLAAHAWVYTESEAPPSSLGLPGNWRLH
CCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEE
REKKAGKVFYALWERQGPSEPTATQ
CCCCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]