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The map label for this gene is metF [H]

Identifier: 146284248

GI number: 146284248

Start: 4266110

End: 4266961

Strand: Direct

Name: metF [H]

Synonym: PST_3936

Alternate gene names: 146284248

Gene position: 4266110-4266961 (Clockwise)

Preceding gene: 146284247

Following gene: 146284249

Centisome position: 93.4

GC content: 64.08

Gene sequence:

>852_bases
ATGACCTCTTCAAATCGCCCTTCCATCAGCTTCGAGTTCTTCCCCACCAAGACCGAAGCCGGGCATGAAAAACTGCTGAA
CGTGGCGCGCCAGCTGGCGGGCTACCAGCCCGATTTCTTCTCCTGCACCTACGGTGCCGGTGGCTCGACGCGCGACCGCA
CGCTCAACACCGTGCTGCAACTCGACGGTGAGGTGAAGATCCCCACCGCCCCGCACCTGTCCTGCGTTGGCGACACCAAG
GCCCAGCTGCGCGAGCTGCTGAACCTCTACAAGAGTGCCGGCATCCAGCGCATCGTCGCCCTGCGCGGCGACCTGCCGTC
GGGTATGGGCATGGCCAGCGGCGAGCTGCGCTACGCCAACGAACTGGTGGAGTTCATCCGCGCCGAGACCGGTGACCACT
TCCATATCGAGATCGCCGCCTACCCGGAAATGCATCCCCAGGCGCGCAACTTCGAGGACGACCTGGCGAACTTCGTGCGC
AAGGCCAAGGCCGGCGCCGACAGTGCGATTACCCAGTACTTCTTCAATGCCGACTGCTACTTCTACTTCGTCGAGCGCGT
GCGCAAGCTCGGGGTTCAGACGCCGGTGGTGCCGGGCATCATGCCGATCACCAATTACAGCAAGCTGGCCCGCTTCTCCG
ATGCCTGTGGCGCGGAGATCCCACGCTGGGTGCGCAAGCAGCTCGAAGCCTATGGCGACGACATGCAGAGCATCCAGGCC
TTCGGCGAGCAGGTGATCACCGAGATGTGCCAGAAGCTGCTGGCCGGCGGTGCGCCGGGCCTGCACTTCTACACGCTGAA
CCAGGCCGAACCGAGCCTGGCGATCTGGAACAACCTCGGGCTGTCGCGCTGA

Upstream 100 bases:

>100_bases
TTCAAGCCGGACAGCTACCGCTACTAAGCAGGTCACGTCACAGGCTGCGAGGCGCCAGGTGCGCTCGCGGCCTGCGGCTT
GCAGCTCAAGGAATTTCTGT

Downstream 100 bases:

>100_bases
GTTCGGCCAGCTCCGGGGAAATACGGAGCTGGCTAATGAACCCGTGACCTGCGACACTGTCAGAGCAATGCGGCGTTAAC
AGCCTCTGTTATACTCCGCG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTSSNRPSISFEFFPTKTEAGHEKLLNVARQLAGYQPDFFSCTYGAGGSTRDRTLNTVLQLDGEVKIPTAPHLSCVGDTK
AQLRELLNLYKSAGIQRIVALRGDLPSGMGMASGELRYANELVEFIRAETGDHFHIEIAAYPEMHPQARNFEDDLANFVR
KAKAGADSAITQYFFNADCYFYFVERVRKLGVQTPVVPGIMPITNYSKLARFSDACGAEIPRWVRKQLEAYGDDMQSIQA
FGEQVITEMCQKLLAGGAPGLHFYTLNQAEPSLAIWNNLGLSR

Sequences:

>Translated_283_residues
MTSSNRPSISFEFFPTKTEAGHEKLLNVARQLAGYQPDFFSCTYGAGGSTRDRTLNTVLQLDGEVKIPTAPHLSCVGDTK
AQLRELLNLYKSAGIQRIVALRGDLPSGMGMASGELRYANELVEFIRAETGDHFHIEIAAYPEMHPQARNFEDDLANFVR
KAKAGADSAITQYFFNADCYFYFVERVRKLGVQTPVVPGIMPITNYSKLARFSDACGAEIPRWVRKQLEAYGDDMQSIQA
FGEQVITEMCQKLLAGGAPGLHFYTLNQAEPSLAIWNNLGLSR
>Mature_282_residues
TSSNRPSISFEFFPTKTEAGHEKLLNVARQLAGYQPDFFSCTYGAGGSTRDRTLNTVLQLDGEVKIPTAPHLSCVGDTKA
QLRELLNLYKSAGIQRIVALRGDLPSGMGMASGELRYANELVEFIRAETGDHFHIEIAAYPEMHPQARNFEDDLANFVRK
AKAGADSAITQYFFNADCYFYFVERVRKLGVQTPVVPGIMPITNYSKLARFSDACGAEIPRWVRKQLEAYGDDMQSIQAF
GEQVITEMCQKLLAGGAPGLHFYTLNQAEPSLAIWNNLGLSR

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=279, Percent_Identity=35.4838709677419, Blast_Score=170, Evalue=1e-42,
Organism=Escherichia coli, GI1790377, Length=274, Percent_Identity=38.6861313868613, Blast_Score=214, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI25147441, Length=280, Percent_Identity=33.2142857142857, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25147436, Length=280, Percent_Identity=33.2142857142857, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321313, Length=287, Percent_Identity=33.4494773519164, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325234, Length=278, Percent_Identity=34.5323741007194, Blast_Score=153, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24662658, Length=233, Percent_Identity=25.7510729613734, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 31403; Mature: 31272

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSNRPSISFEFFPTKTEAGHEKLLNVARQLAGYQPDFFSCTYGAGGSTRDRTLNTVLQ
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHH
LDGEVKIPTAPHLSCVGDTKAQLRELLNLYKSAGIQRIVALRGDLPSGMGMASGELRYAN
CCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
ELVEFIRAETGDHFHIEIAAYPEMHPQARNFEDDLANFVRKAKAGADSAITQYFFNADCY
HHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHH
FYFVERVRKLGVQTPVVPGIMPITNYSKLARFSDACGAEIPRWVRKQLEAYGDDMQSIQA
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHH
FGEQVITEMCQKLLAGGAPGLHFYTLNQAEPSLAIWNNLGLSR
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEHHCCCCCCC
>Mature Secondary Structure 
TSSNRPSISFEFFPTKTEAGHEKLLNVARQLAGYQPDFFSCTYGAGGSTRDRTLNTVLQ
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHH
LDGEVKIPTAPHLSCVGDTKAQLRELLNLYKSAGIQRIVALRGDLPSGMGMASGELRYAN
CCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
ELVEFIRAETGDHFHIEIAAYPEMHPQARNFEDDLANFVRKAKAGADSAITQYFFNADCY
HHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHH
FYFVERVRKLGVQTPVVPGIMPITNYSKLARFSDACGAEIPRWVRKQLEAYGDDMQSIQA
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHH
FGEQVITEMCQKLLAGGAPGLHFYTLNQAEPSLAIWNNLGLSR
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]