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The map label for this gene is ada [H]

Identifier: 146283938

GI number: 146283938

Start: 3910122

End: 3911162

Strand: Direct

Name: ada [H]

Synonym: PST_3621

Alternate gene names: 146283938

Gene position: 3910122-3911162 (Clockwise)

Preceding gene: 146283936

Following gene: 146283939

Centisome position: 85.61

GC content: 72.43

Gene sequence:

>1041_bases
ATGCTCGATGAAGACCGTTGCTGGCAGGCGCTGTGCGAGCGCGATGCCGCCTTCGATGGGCGTTTCGTCTTCGCCGTGCG
CTCGACCGGCATCTTCTGCCGGCCCAGCTGCCCGGCCCGCCGCCCCGCCCGCGAGCGGGTGGCCTTTTTTGCCGATGGCG
AAGCGGCCCGCGCCGCTGGCTTTCGTCCCTGCCGACGCTGCTCGCCAATGGGGCCAAGCCCGAGCGAGCAGCTGGACGCG
CTGGTCGTCGCCGCCTGTCGCCTGCTCGACGAAGCCACCGGGCCCATGACACTGCCCCAGCTGGCTGCACGTATCGGCCT
GTCGCCGACGCATCTGGCCCGCGCCTTCAAGGCCCGCACCGGTCTGACGCCCCATGCCTGGGCAACCGCGCGCCGGCGTG
AACGCCTCGATGCCCAACTGCCCGAGGCCGGTTCGGTACTGGATGCCGCCCTTGCCGTCGGTTACTCCGGCACCCGTGCG
CCCTATGCCGAGGCACGGGCGCTGACCCCGGCACAACGGCGTCGCAAGGGCGCCGGCGAAACCCTGCGCTATGCCCTGGC
GCCCTGTCCGCTCGGCCTGCTGCTGCTTGCCGCCAGCGACAAGGGCATCTGTGCCCTGCTGTTCGGCGATGACGAAGGCG
CGCTGGAGCAGGAGCTGCGCTCGCGCTTCGCCGCCGCCGACCTGCGCCGCGATCAGGCCGGGCTGGGCGACTGGCTGCAG
GCGATCGTCAGCCAGCTGGAAGAACCGCAGCGCGCCACCCAGCTGCCTCTCGACCTGCGCGGCACAGCCTTTCAGCAGCG
CGTCTGGCAGGCGCTGCAGCAGATTCCCGCCGGCGAGACACGCCGCTACGGCGAGCTGGCGGCGACCCTCGGCAGCCACG
CGCGGGCGGTGGCGCGTGCCTGCGCCAGCAACCCGATCGGCCTGCTGGTACCCTGCCACCGCGTGGTCGGCAGCCAGAAC
GCGCTGACCGGCTACCGCTGGGGCCTGGCGCGCAAGGCCAGGCTGCTCGATCGGGAAGGGCAAGGCGAGTCGGGAGCCTG
A

Upstream 100 bases:

>100_bases
CCGGCTTCCATGCCTGCCATCGAGTTTGAAAGCGCGCTTTGGATTGGCGCACAGCACGTTCAGCGCCGACAATCGAGCCA
TCATTCCCTGGAGCACCGTC

Downstream 100 bases:

>100_bases
GCGGATTTCAGCGGTCGGATATTGCAGTGCCGCCCACCGCAGGAAGCCGTCATGACCGAACTGAAGCGTTACCGCATCCA
CTACCGCATCAATGGCGAGC

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MLDEDRCWQALCERDAAFDGRFVFAVRSTGIFCRPSCPARRPARERVAFFADGEAARAAGFRPCRRCSPMGPSPSEQLDA
LVVAACRLLDEATGPMTLPQLAARIGLSPTHLARAFKARTGLTPHAWATARRRERLDAQLPEAGSVLDAALAVGYSGTRA
PYAEARALTPAQRRRKGAGETLRYALAPCPLGLLLLAASDKGICALLFGDDEGALEQELRSRFAAADLRRDQAGLGDWLQ
AIVSQLEEPQRATQLPLDLRGTAFQQRVWQALQQIPAGETRRYGELAATLGSHARAVARACASNPIGLLVPCHRVVGSQN
ALTGYRWGLARKARLLDREGQGESGA

Sequences:

>Translated_346_residues
MLDEDRCWQALCERDAAFDGRFVFAVRSTGIFCRPSCPARRPARERVAFFADGEAARAAGFRPCRRCSPMGPSPSEQLDA
LVVAACRLLDEATGPMTLPQLAARIGLSPTHLARAFKARTGLTPHAWATARRRERLDAQLPEAGSVLDAALAVGYSGTRA
PYAEARALTPAQRRRKGAGETLRYALAPCPLGLLLLAASDKGICALLFGDDEGALEQELRSRFAAADLRRDQAGLGDWLQ
AIVSQLEEPQRATQLPLDLRGTAFQQRVWQALQQIPAGETRRYGELAATLGSHARAVARACASNPIGLLVPCHRVVGSQN
ALTGYRWGLARKARLLDREGQGESGA
>Mature_346_residues
MLDEDRCWQALCERDAAFDGRFVFAVRSTGIFCRPSCPARRPARERVAFFADGEAARAAGFRPCRRCSPMGPSPSEQLDA
LVVAACRLLDEATGPMTLPQLAARIGLSPTHLARAFKARTGLTPHAWATARRRERLDAQLPEAGSVLDAALAVGYSGTRA
PYAEARALTPAQRRRKGAGETLRYALAPCPLGLLLLAASDKGICALLFGDDEGALEQELRSRFAAADLRRDQAGLGDWLQ
AIVSQLEEPQRATQLPLDLRGTAFQQRVWQALQQIPAGETRRYGELAATLGSHARAVARACASNPIGLLVPCHRVVGSQN
ALTGYRWGLARKARLLDREGQGESGA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=110, Percent_Identity=40, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1788542, Length=347, Percent_Identity=46.3976945244957, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI1787596, Length=88, Percent_Identity=50, Blast_Score=85, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI115533068, Length=81, Percent_Identity=55.5555555555556, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI115533070, Length=81, Percent_Identity=55.5555555555556, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320001, Length=91, Percent_Identity=45.0549450549451, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI17137554, Length=175, Percent_Identity=30.8571428571429, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 37377; Mature: 37377

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDEDRCWQALCERDAAFDGRFVFAVRSTGIFCRPSCPARRPARERVAFFADGEAARAAG
CCCHHHHHHHHHHHCCCCCCEEEEEEECCCEEECCCCCCCCCHHHHEEEEECCCHHHHCC
FRPCRRCSPMGPSPSEQLDALVVAACRLLDEATGPMTLPQLAARIGLSPTHLARAFKART
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHC
GLTPHAWATARRRERLDAQLPEAGSVLDAALAVGYSGTRAPYAEARALTPAQRRRKGAGE
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHCCCCH
TLRYALAPCPLGLLLLAASDKGICALLFGDDEGALEQELRSRFAAADLRRDQAGLGDWLQ
HHHHHHCCCCHHEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
AIVSQLEEPQRATQLPLDLRGTAFQQRVWQALQQIPAGETRRYGELAATLGSHARAVARA
HHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
CASNPIGLLVPCHRVVGSQNALTGYRWGLARKARLLDREGQGESGA
HHCCCCEEEEHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLDEDRCWQALCERDAAFDGRFVFAVRSTGIFCRPSCPARRPARERVAFFADGEAARAAG
CCCHHHHHHHHHHHCCCCCCEEEEEEECCCEEECCCCCCCCCHHHHEEEEECCCHHHHCC
FRPCRRCSPMGPSPSEQLDALVVAACRLLDEATGPMTLPQLAARIGLSPTHLARAFKART
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHC
GLTPHAWATARRRERLDAQLPEAGSVLDAALAVGYSGTRAPYAEARALTPAQRRRKGAGE
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHCCCCH
TLRYALAPCPLGLLLLAASDKGICALLFGDDEGALEQELRSRFAAADLRRDQAGLGDWLQ
HHHHHHCCCCHHEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
AIVSQLEEPQRATQLPLDLRGTAFQQRVWQALQQIPAGETRRYGELAATLGSHARAVARA
HHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
CASNPIGLLVPCHRVVGSQNALTGYRWGLARKARLLDREGQGESGA
HHCCCCEEEEHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]