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The map label for this gene is oprM [H]

Identifier: 146283936

GI number: 146283936

Start: 3907531

End: 3908997

Strand: Direct

Name: oprM [H]

Synonym: PST_3619

Alternate gene names: 146283936

Gene position: 3907531-3908997 (Clockwise)

Preceding gene: 146283935

Following gene: 146283938

Centisome position: 85.55

GC content: 72.53

Gene sequence:

>1467_bases
ATGACTGCCAAGGCCCTGGCCGCTCCGCTGCTGCTCGCCCTGGCCCTGAGTGCCTGCTCCAGCGCACCGCAGCACCCGGC
GCCGCAACTGCCGGATACCTGGTTCAGCGCCGCCGGCGCCCACCCGGCCGATGCCGAGTCGCTGGCCAGCTGGTGGCGTC
AGTTCGACGACCCGCAGCTCACCGCGCTGGTCCACCGCGCCATGCAGCAGAACCACGATGTTCGCCTGGCCATGGCGCGG
GTCGACAGCGCCCGCGCGCAGCTGCGCCAGTCGCGCGCCGGCCTGCTGCCGAGCTTCGACCTGCCGGGCTCGGCCAGCCG
CCAGTGGATCGAGAACGACCAGGACGCCGAACCCGGCAGCCCGCTGGCCGAGTTCATCCCGGATGACGACCCGATCGCCT
TCGACAGCTGGGAACTGGCGCTGCAGGCGACCTGGGAACTGGACCTGTTCGGCGCCACCCGCGCCCGACGCGACAGCGCC
GCCCAGCAACTGCGTTCGGCCGAGGCGCAGACGGTGGCCGCGCGCCTGGCCGTGGCCGCCAACACCGCGCAGGGCTATCT
GCAACTGCGCGCGCTGCAGGGGCAGCGCGCGCTCTTGGTCGAAGGCATCGAGGTGGCGCGCGAACTGGAGCGCATCGCCG
GACTGCTGTTTCACGCCGGCGAGGTGACCCGGCTGGACGTGGAGGCCACATCCGCCGAACGCGCATCGCTGGAAGCCGAT
CTGGACGAGCTGGACATCCACCTGGCCGAAGCGCAGCTGGCGCTGGACACCCTGCTCGCCGCGCCGCCGGGCAGTAGCGC
CGTCCAGCTCGCCGCCAGTACGGCGGTGCCGTTGGCCGAATCGCGCATCGCCGCCGGTCAGCCCATCGACCTGCTGCGCC
GCCGTCCGGACCTGATTGCCGAAGCGGCACGGCTGGACGCCGCCGAGCTGCAATCGCTGGCCGCTCGCCGCGACCTGTTT
CCCAAGCTGGCGGTGCAGGCCGCGGTGGGCCGCTCCGGCTTCGCGCTGGGCGACGCCGTTTCCAGCGCTTCGAACTTCGC
CCGGGTCGGCGCCACCTTCGGCCTGCCGCTGCTGGACTATGCAAACCGTGGCGCCGCCATCGAACTGGCCGATGCCGAAG
GCGAGACCGCCTACATCGCCTTCGAGCAGGCCCTGGCCCGCGCGCTGGAGGAGGTCGAACGCGGCCTGACACGCATTGCC
GGCCAGCAACGTCGGCACGCCGCCCTCAGCCGCACCCGCGATCACCGCGAGCGCGCACACCGGCTGGCACAGCGCAGCTA
CGAGCTGGGCGAGGCGAACCTGAGCGAGGTGCTGGACGCCCAGCGCGGCGCACTGCAGGCGCGCCAGCGGGCACTGGAAG
GCCGCACCGCACTGGCGACCGCGCAGGTGGCGTTGTACGTGGCACTGGGTGGTGGCTGGAACGCCGCGGATGGTGCGGCG
GTCTCTGAGGATCAGTCAGCGCCCTGA

Upstream 100 bases:

>100_bases
TGACCATCATGGGCGGCCTGGGCTTCGCCACGCTGCTGACGCTGCTGGCCGTGCCCTGCCTGTACCTGCTGCTGATGAAA
GTGCGCCCGGAGGAAACCGC

Downstream 100 bases:

>100_bases
GGGGTCGGTTGGTGACAATGCAAGCCGGCTGGCGCATGGGCTACTTGGCAGCGGGCGGGCCGAGTAGCCAAAACCATCAC
CCCTTCATCCAGGGCGGCTC

Product: outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTAKALAAPLLLALALSACSSAPQHPAPQLPDTWFSAAGAHPADAESLASWWRQFDDPQLTALVHRAMQQNHDVRLAMAR
VDSARAQLRQSRAGLLPSFDLPGSASRQWIENDQDAEPGSPLAEFIPDDDPIAFDSWELALQATWELDLFGATRARRDSA
AQQLRSAEAQTVAARLAVAANTAQGYLQLRALQGQRALLVEGIEVARELERIAGLLFHAGEVTRLDVEATSAERASLEAD
LDELDIHLAEAQLALDTLLAAPPGSSAVQLAASTAVPLAESRIAAGQPIDLLRRRPDLIAEAARLDAAELQSLAARRDLF
PKLAVQAAVGRSGFALGDAVSSASNFARVGATFGLPLLDYANRGAAIELADAEGETAYIAFEQALARALEEVERGLTRIA
GQQRRHAALSRTRDHRERAHRLAQRSYELGEANLSEVLDAQRGALQARQRALEGRTALATAQVALYVALGGGWNAADGAA
VSEDQSAP

Sequences:

>Translated_488_residues
MTAKALAAPLLLALALSACSSAPQHPAPQLPDTWFSAAGAHPADAESLASWWRQFDDPQLTALVHRAMQQNHDVRLAMAR
VDSARAQLRQSRAGLLPSFDLPGSASRQWIENDQDAEPGSPLAEFIPDDDPIAFDSWELALQATWELDLFGATRARRDSA
AQQLRSAEAQTVAARLAVAANTAQGYLQLRALQGQRALLVEGIEVARELERIAGLLFHAGEVTRLDVEATSAERASLEAD
LDELDIHLAEAQLALDTLLAAPPGSSAVQLAASTAVPLAESRIAAGQPIDLLRRRPDLIAEAARLDAAELQSLAARRDLF
PKLAVQAAVGRSGFALGDAVSSASNFARVGATFGLPLLDYANRGAAIELADAEGETAYIAFEQALARALEEVERGLTRIA
GQQRRHAALSRTRDHRERAHRLAQRSYELGEANLSEVLDAQRGALQARQRALEGRTALATAQVALYVALGGGWNAADGAA
VSEDQSAP
>Mature_487_residues
TAKALAAPLLLALALSACSSAPQHPAPQLPDTWFSAAGAHPADAESLASWWRQFDDPQLTALVHRAMQQNHDVRLAMARV
DSARAQLRQSRAGLLPSFDLPGSASRQWIENDQDAEPGSPLAEFIPDDDPIAFDSWELALQATWELDLFGATRARRDSAA
QQLRSAEAQTVAARLAVAANTAQGYLQLRALQGQRALLVEGIEVARELERIAGLLFHAGEVTRLDVEATSAERASLEADL
DELDIHLAEAQLALDTLLAAPPGSSAVQLAASTAVPLAESRIAAGQPIDLLRRRPDLIAEAARLDAAELQSLAARRDLFP
KLAVQAAVGRSGFALGDAVSSASNFARVGATFGLPLLDYANRGAAIELADAEGETAYIAFEQALARALEEVERGLTRIAG
QQRRHAALSRTRDHRERAHRLAQRSYELGEANLSEVLDAQRGALQARQRALEGRTALATAQVALYVALGGGWNAADGAAV
SEDQSAP

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=475, Percent_Identity=26.3157894736842, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI2367348, Length=435, Percent_Identity=24.367816091954, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52003; Mature: 51871

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKALAAPLLLALALSACSSAPQHPAPQLPDTWFSAAGAHPADAESLASWWRQFDDPQL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCHH
TALVHRAMQQNHDVRLAMARVDSARAQLRQSRAGLLPSFDLPGSASRQWIENDQDAEPGS
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCC
PLAEFIPDDDPIAFDSWELALQATWELDLFGATRARRDSAAQQLRSAEAQTVAARLAVAA
CHHHHCCCCCCCEECCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NTAQGYLQLRALQGQRALLVEGIEVARELERIAGLLFHAGEVTRLDVEATSAERASLEAD
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHCC
LDELDIHLAEAQLALDTLLAAPPGSSAVQLAASTAVPLAESRIAAGQPIDLLRRRPDLIA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHCCCCCHHHHHCCHHHHH
EAARLDAAELQSLAARRDLFPKLAVQAAVGRSGFALGDAVSSASNFARVGATFGLPLLDY
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
ANRGAAIELADAEGETAYIAFEQALARALEEVERGLTRIAGQQRRHAALSRTRDHRERAH
CCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLAQRSYELGEANLSEVLDAQRGALQARQRALEGRTALATAQVALYVALGGGWNAADGAA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEEEEEEECCCCCCCCCCC
VSEDQSAP
CCCCCCCC
>Mature Secondary Structure 
TAKALAAPLLLALALSACSSAPQHPAPQLPDTWFSAAGAHPADAESLASWWRQFDDPQL
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCHH
TALVHRAMQQNHDVRLAMARVDSARAQLRQSRAGLLPSFDLPGSASRQWIENDQDAEPGS
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCC
PLAEFIPDDDPIAFDSWELALQATWELDLFGATRARRDSAAQQLRSAEAQTVAARLAVAA
CHHHHCCCCCCCEECCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NTAQGYLQLRALQGQRALLVEGIEVARELERIAGLLFHAGEVTRLDVEATSAERASLEAD
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHCC
LDELDIHLAEAQLALDTLLAAPPGSSAVQLAASTAVPLAESRIAAGQPIDLLRRRPDLIA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHCCCCCHHHHHCCHHHHH
EAARLDAAELQSLAARRDLFPKLAVQAAVGRSGFALGDAVSSASNFARVGATFGLPLLDY
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
ANRGAAIELADAEGETAYIAFEQALARALEEVERGLTRIAGQQRRHAALSRTRDHRERAH
CCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLAQRSYELGEANLSEVLDAQRGALQARQRALEGRTALATAQVALYVALGGGWNAADGAA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEEEEEEECCCCCCCCCCC
VSEDQSAP
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]