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The map label for this gene is rutF

Identifier: 146283919

GI number: 146283919

Start: 3889369

End: 3889893

Strand: Direct

Name: rutF

Synonym: PST_3602

Alternate gene names: 146283919

Gene position: 3889369-3889893 (Clockwise)

Preceding gene: 146283918

Following gene: 146283920

Centisome position: 85.15

GC content: 66.29

Gene sequence:

>525_bases
ATGCAACTGGCTCAATGCGAAACCACCAGTCAATTCAGCGCTCCAGTGATTCGTGAGGATTATCGTGATGCCATGGCGCG
CCTGGCCGCGGCGGTCAACATCATTACCACCGACGGCCCCAGCGGCCGTGCCGGCTTCACCGCCACCGCGGTGTGCAGCG
TCACCGACGAACCGCCGACGCTGCTGGTGTGCCTGAACCGCAGCGCATCGGCACATCCCATCGTCACCGCCAACGGGCAG
CTGTGCGTGAACACCCTGGCCGGTGCGCAGCGGGACCTGTCCAACCTGTTCGGCGGCAAGACGCCCATGGCCGAGCGCTT
CGCCGCAGCGCAATGGAGCACCGGCGTGACCGGTTCGCCGCTGCTCGACGGCGCCACAGTGTCGTTCGACTGCCGCATCA
GCCACAGCGCCAGCGTCGGCACCCACGACATCCTCTACTGCGAGGTGCTCGCCGTGTACCGCCGCGACAGCAGCGATGCG
CTGGTGTACTTCGGCCGCAACTACCACGGGCTTTCAAGCAGCTGA

Upstream 100 bases:

>100_bases
CTGATCAACCTCGGCTACGGCGATGCCAGCAAGCTGCGCGACCGCCTGCCACGCCTGCCCTTCGACCGCGCCTGCGTCCT
CGCCTGATGGAGAACCGACC

Downstream 100 bases:

>100_bases
GCAAACGAATGCTCTCCCATCGGGCGGGCTTGGGCGCATCCACGGTGCAGAATGCGGTGGGCTGAAGCCCACCCTACAAA
ACCTCGAAGCCTTCTGTGCC

Product: NAD(P)H-flavin oxidoreductase

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MQLAQCETTSQFSAPVIREDYRDAMARLAAAVNIITTDGPSGRAGFTATAVCSVTDEPPTLLVCLNRSASAHPIVTANGQ
LCVNTLAGAQRDLSNLFGGKTPMAERFAAAQWSTGVTGSPLLDGATVSFDCRISHSASVGTHDILYCEVLAVYRRDSSDA
LVYFGRNYHGLSSS

Sequences:

>Translated_174_residues
MQLAQCETTSQFSAPVIREDYRDAMARLAAAVNIITTDGPSGRAGFTATAVCSVTDEPPTLLVCLNRSASAHPIVTANGQ
LCVNTLAGAQRDLSNLFGGKTPMAERFAAAQWSTGVTGSPLLDGATVSFDCRISHSASVGTHDILYCEVLAVYRRDSSDA
LVYFGRNYHGLSSS
>Mature_174_residues
MQLAQCETTSQFSAPVIREDYRDAMARLAAAVNIITTDGPSGRAGFTATAVCSVTDEPPTLLVCLNRSASAHPIVTANGQ
LCVNTLAGAQRDLSNLFGGKTPMAERFAAAQWSTGVTGSPLLDGATVSFDCRISHSASVGTHDILYCEVLAVYRRDSSDA
LVYFGRNYHGLSSS

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily

Homologues:

Organism=Escherichia coli, GI87081819, Length=156, Percent_Identity=65.3846153846154, Blast_Score=212, Evalue=9e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTF_PSEU5 (A4VQH9)

Other databases:

- EMBL:   CP000304
- RefSeq:   YP_001174072.1
- ProteinModelPortal:   A4VQH9
- STRING:   A4VQH9
- GeneID:   5096563
- GenomeReviews:   CP000304_GR
- KEGG:   psa:PST_3602
- eggNOG:   COG1853
- HOGENOM:   HBG730363
- OMA:   NRSARAR
- PhylomeDB:   A4VQH9
- BioCyc:   PSTU379731:PST_3602-MONOMER
- HAMAP:   MF_00833
- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- Gene3D:   G3DSA:2.30.110.10
- SMART:   SM00903
- TIGRFAMs:   TIGR03615

Pfam domain/function: PF01613 Flavin_Reduct; SSF50475 FMN_binding

EC number: =1.5.1.29

Molecular weight: Translated: 18366; Mature: 18366

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLAQCETTSQFSAPVIREDYRDAMARLAAAVNIITTDGPSGRAGFTATAVCSVTDEPPT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEEEECCCCCE
LLVCLNRSASAHPIVTANGQLCVNTLAGAQRDLSNLFGGKTPMAERFAAAQWSTGVTGSP
EEEEECCCCCCCCEEEECCCEEEEHHHCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCC
LLDGATVSFDCRISHSASVGTHDILYCEVLAVYRRDSSDALVYFGRNYHGLSSS
CCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MQLAQCETTSQFSAPVIREDYRDAMARLAAAVNIITTDGPSGRAGFTATAVCSVTDEPPT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEEEECCCCCE
LLVCLNRSASAHPIVTANGQLCVNTLAGAQRDLSNLFGGKTPMAERFAAAQWSTGVTGSP
EEEEECCCCCCCCEEEECCCEEEEHHHCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCC
LLDGATVSFDCRISHSASVGTHDILYCEVLAVYRRDSSDALVYFGRNYHGLSSS
CCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA