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The map label for this gene is trxB [H]

Identifier: 146283875

GI number: 146283875

Start: 3846544

End: 3847827

Strand: Direct

Name: trxB [H]

Synonym: PST_3557

Alternate gene names: 146283875

Gene position: 3846544-3847827 (Clockwise)

Preceding gene: 146283872

Following gene: 146283876

Centisome position: 84.22

GC content: 67.68

Gene sequence:

>1284_bases
ATGGTTCAGCCGGCGCCGGGTGTGGCGAGGCTGGGATACAGCGAGGGGACCAGTGCACCGCGAGCGGAGCCGCAGGCTGG
TCTTTATTGGAATGGCGGTGACCGATCGGTACGGGTTGGACCCTACGGTCACCGAGCCGCGCAGTTTTGGCCATCGACGG
CTCTGGCACAAGGGTTGAAGCTCCAACCAGCTCTCAAAACCGCACATTGCATCCATCGGCCGGACCGAACTTTCTCGTCA
CGCCCCCCTTCTACTGGCAACCACCCGCCACCGGCCAACGCCATGCCCCCCGAGCAAGCGAACCCGCAAACTGCCGTGAC
CGACTGCCTGATCGTCGGCGGCGGTCCCGCCGGCCTCACCGCCGCGCTGTACCTGGCGCGCTTCCGACGCAGCTGTCTGG
TGGTCGATGCTGGCAGCAGCCGCGCCGCGTGGATACCGCGGTCGCGCAACTACCCGGGCTTTCCACCGGGCATCAGCGGC
AATGACCTGCTGGCCAGGCTGCGCGAACAGGCCGCTGGCTACGGCGCACGCCTGGAGCACGGCCAGGTCGAGCACATCGA
GCCGCATGTCGAGGGCTTCAGCGTGCGTTACGGCGAGCGCAGCTGCATCACCCGGCGGATCATCCTCGCCACCGGTATCG
AGGACCGGCTGCCGGACATGCCCGACATCGAGCCGGCCATTCACGCCGGCAAGGTGCGGCTGTGCGCGATCTGCGATGGC
TATGAGGTCGACGGCGACAACGTCGCCGTGTATGGCGAAGCGGAAAGCGCCATCACCCACGCAGTGTTCCTTCGCACCTT
CACCGACCGGGTCACCGTGGTGGTGCACGGCGACCAGGCGGCATGCGAACAGGCCATCGCCCTGGCCGAGCACTACGCCA
TCCGCGTGATCGGCGACCGGGTCGAATCCATGCAGCCCTGCGAGAACGGCATCGACCTGCTGACCTGCCGCGGCGACCGG
CACAATTTCGACATCATCTACCCCAGCCTGGGCGCACGCTTTCGCTCGGAGCTGGCGCTGCAGCTGGGTCTGGACTGCGA
CGAATGCGGCGGTGTGAAGGTGGACCAACACCTGCAGACCTCGCTGCGCGGCCTCTATGCCATCGGTGACGTGACCCTGG
GCCTGAAGCAGATCAGCGTCGCCATCGGCCAGGCCGCGCAGGCCGCGACCGCGGTGCACAACAGCCTGGAGGCCAACCCC
TGGGGCGGCTCGCGCCAGGCCGCGGACCAGCCCGGCGCCGCGACCGCCCAGAACGACAACGAAAGCCTTCCCGATCAACC
TTGA

Upstream 100 bases:

>100_bases
GGGCGCAACAACCAGAGACTTGAACACGATCTACCCCCACGGGAAATAAGCCCGACACGGCGCCTCGTGAGCACCGTTCG
AGTGGTGTTTGCAATTCGAT

Downstream 100 bases:

>100_bases
CGGGCGGCCGAGGGTCTAGGCTTATACAAGCCTCGCCTGGAGCCTCCCAATGTCCCTCAGTGTCTTCGACCTGTTCAAGA
TCGGTATCGGCCCGTCCAGT

Product: thioredoxin reductase

Products: NA

Alternate protein names: TRXR; General stress protein 35; GSP35 [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MVQPAPGVARLGYSEGTSAPRAEPQAGLYWNGGDRSVRVGPYGHRAAQFWPSTALAQGLKLQPALKTAHCIHRPDRTFSS
RPPSTGNHPPPANAMPPEQANPQTAVTDCLIVGGGPAGLTAALYLARFRRSCLVVDAGSSRAAWIPRSRNYPGFPPGISG
NDLLARLREQAAGYGARLEHGQVEHIEPHVEGFSVRYGERSCITRRIILATGIEDRLPDMPDIEPAIHAGKVRLCAICDG
YEVDGDNVAVYGEAESAITHAVFLRTFTDRVTVVVHGDQAACEQAIALAEHYAIRVIGDRVESMQPCENGIDLLTCRGDR
HNFDIIYPSLGARFRSELALQLGLDCDECGGVKVDQHLQTSLRGLYAIGDVTLGLKQISVAIGQAAQAATAVHNSLEANP
WGGSRQAADQPGAATAQNDNESLPDQP

Sequences:

>Translated_427_residues
MVQPAPGVARLGYSEGTSAPRAEPQAGLYWNGGDRSVRVGPYGHRAAQFWPSTALAQGLKLQPALKTAHCIHRPDRTFSS
RPPSTGNHPPPANAMPPEQANPQTAVTDCLIVGGGPAGLTAALYLARFRRSCLVVDAGSSRAAWIPRSRNYPGFPPGISG
NDLLARLREQAAGYGARLEHGQVEHIEPHVEGFSVRYGERSCITRRIILATGIEDRLPDMPDIEPAIHAGKVRLCAICDG
YEVDGDNVAVYGEAESAITHAVFLRTFTDRVTVVVHGDQAACEQAIALAEHYAIRVIGDRVESMQPCENGIDLLTCRGDR
HNFDIIYPSLGARFRSELALQLGLDCDECGGVKVDQHLQTSLRGLYAIGDVTLGLKQISVAIGQAAQAATAVHNSLEANP
WGGSRQAADQPGAATAQNDNESLPDQP
>Mature_427_residues
MVQPAPGVARLGYSEGTSAPRAEPQAGLYWNGGDRSVRVGPYGHRAAQFWPSTALAQGLKLQPALKTAHCIHRPDRTFSS
RPPSTGNHPPPANAMPPEQANPQTAVTDCLIVGGGPAGLTAALYLARFRRSCLVVDAGSSRAAWIPRSRNYPGFPPGISG
NDLLARLREQAAGYGARLEHGQVEHIEPHVEGFSVRYGERSCITRRIILATGIEDRLPDMPDIEPAIHAGKVRLCAICDG
YEVDGDNVAVYGEAESAITHAVFLRTFTDRVTVVVHGDQAACEQAIALAEHYAIRVIGDRVESMQPCENGIDLLTCRGDR
HNFDIIYPSLGARFRSELALQLGLDCDECGGVKVDQHLQTSLRGLYAIGDVTLGLKQISVAIGQAAQAATAVHNSLEANP
WGGSRQAADQPGAATAQNDNESLPDQP

Specific function: Unknown

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787114, Length=205, Percent_Identity=27.3170731707317, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320560, Length=284, Percent_Identity=27.112676056338, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321898, Length=285, Percent_Identity=26.6666666666667, Blast_Score=76, Evalue=9e-15,

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR005982 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.8.1.9 [H]

Molecular weight: Translated: 45636; Mature: 45636

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQPAPGVARLGYSEGTSAPRAEPQAGLYWNGGDRSVRVGPYGHRAAQFWPSTALAQGLK
CCCCCCCHHHCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCHHHHHCCCHHHHCCCC
LQPALKTAHCIHRPDRTFSSRPPSTGNHPPPANAMPPEQANPQTAVTDCLIVGGGPAGLT
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHH
AALYLARFRRSCLVVDAGSSRAAWIPRSRNYPGFPPGISGNDLLARLREQAAGYGARLEH
HHHHHHHHCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
GQVEHIEPHVEGFSVRYGERSCITRRIILATGIEDRLPDMPDIEPAIHAGKVRLCAICDG
CCCCCCCCCCCCEEEECCCHHHHHEEEEEEECCHHHCCCCCCCCHHHCCCCEEEEEEECC
YEVDGDNVAVYGEAESAITHAVFLRTFTDRVTVVVHGDQAACEQAIALAEHYAIRVIGDR
EEECCCCEEEEECHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHEEEEEHHH
VESMQPCENGIDLLTCRGDRHNFDIIYPSLGARFRSELALQLGLDCDECGGVKVDQHLQT
HHHCCCHHCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHCCCCHHHCCCCCHHHHHHH
SLRGLYAIGDVTLGLKQISVAIGQAAQAATAVHNSLEANPWGGSRQAADQPGAATAQNDN
HHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
ESLPDQP
CCCCCCC
>Mature Secondary Structure
MVQPAPGVARLGYSEGTSAPRAEPQAGLYWNGGDRSVRVGPYGHRAAQFWPSTALAQGLK
CCCCCCCHHHCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCHHHHHCCCHHHHCCCC
LQPALKTAHCIHRPDRTFSSRPPSTGNHPPPANAMPPEQANPQTAVTDCLIVGGGPAGLT
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHH
AALYLARFRRSCLVVDAGSSRAAWIPRSRNYPGFPPGISGNDLLARLREQAAGYGARLEH
HHHHHHHHCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
GQVEHIEPHVEGFSVRYGERSCITRRIILATGIEDRLPDMPDIEPAIHAGKVRLCAICDG
CCCCCCCCCCCCEEEECCCHHHHHEEEEEEECCHHHCCCCCCCCHHHCCCCEEEEEEECC
YEVDGDNVAVYGEAESAITHAVFLRTFTDRVTVVVHGDQAACEQAIALAEHYAIRVIGDR
EEECCCCEEEEECHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHEEEEEHHH
VESMQPCENGIDLLTCRGDRHNFDIIYPSLGARFRSELALQLGLDCDECGGVKVDQHLQT
HHHCCCHHCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHCCCCHHHCCCCCHHHHHHH
SLRGLYAIGDVTLGLKQISVAIGQAAQAATAVHNSLEANPWGGSRQAADQPGAATAQNDN
HHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
ESLPDQP
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 9298659 [H]