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The map label for this gene is dnrS [H]

Identifier: 146283827

GI number: 146283827

Start: 3800887

End: 3801564

Strand: Direct

Name: dnrS [H]

Synonym: PST_3510

Alternate gene names: 146283827

Gene position: 3800887-3801564 (Clockwise)

Preceding gene: 146283823

Following gene: 146283828

Centisome position: 83.22

GC content: 64.31

Gene sequence:

>678_bases
ATGCTCACCGAAAAGACCCTGGTAGCGGAGCTACGCCGCCATCATCTGTTCAGCCGGCTGCCGGACGCCGCGCTGCAGGA
GGTATGCGCTTCGGCCAACCTCAAGCGCCTGCCGGCCGGAGCCTCGCTGTTTCACCAGGGCGACAAGGCCGACCGCTTCT
ATTTCCTCTTCAGTGGCCAGATCAAGCTGCATCGGGTGGTCTGCGACGGTCAGGAGAAGCTGGTGGAAGTGATGCGCGCC
GGCGAGTCGTTCGCCGAGGCACTGCTGTTCAAGGGCACGCCCTGCTATCCGGTCAGCGCCACCGCCCTCAAGGCCAGCCT
GGTGGCAAGCCTGAACGGGCCCCACTACCGACGCATCCTCGAGCAGCAGCCGGACATCTGCCTGGACATCCTCGCCACCC
TCAGCATCCGTCTGCACCAGCGCATGACCGAGATCGACACCCTGACCCTGGCCAACGCCAGCCATCGGGTGGTGCGCTTT
CTCGCGCAGTCGCAGCAGGACGACAGCGGCGTGGTGGTGCTCGATGTGCCGAAACGGCTGATCGCTTCGAAGCTGGGCAT
CCAGCCGGAAACCTTCTCGCGCATCCTGCATCGACTGATCGACGCCGGAACCATTTCCGTACAGCGTCGGCGCATCGAGA
TCCTCGACAACCGCAAGCTGGCCGCCTATGACGAATGA

Upstream 100 bases:

>100_bases
CCTGCGTGACGCGCAGACTTGTCCCTGATCAAGGAACCTCCCGCGGCGGCGCTCCTAGGATGCAGCCAGATAAGACCCGG
CCCAGCCCGGGGGAGGAACC

Downstream 100 bases:

>100_bases
CCCGCGGCATGTCCGCCCGCCAACCCACACCACTGCCGGGAGCGCGCGATGACCGTCACCCCCATCCCCCTGGTCTATTC
CTGTTCCGGCTGCTCGAACG

Product: transcriptional regulator DnrS

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MLTEKTLVAELRRHHLFSRLPDAALQEVCASANLKRLPAGASLFHQGDKADRFYFLFSGQIKLHRVVCDGQEKLVEVMRA
GESFAEALLFKGTPCYPVSATALKASLVASLNGPHYRRILEQQPDICLDILATLSIRLHQRMTEIDTLTLANASHRVVRF
LAQSQQDDSGVVVLDVPKRLIASKLGIQPETFSRILHRLIDAGTISVQRRRIEILDNRKLAAYDE

Sequences:

>Translated_225_residues
MLTEKTLVAELRRHHLFSRLPDAALQEVCASANLKRLPAGASLFHQGDKADRFYFLFSGQIKLHRVVCDGQEKLVEVMRA
GESFAEALLFKGTPCYPVSATALKASLVASLNGPHYRRILEQQPDICLDILATLSIRLHQRMTEIDTLTLANASHRVVRF
LAQSQQDDSGVVVLDVPKRLIASKLGIQPETFSRILHRLIDAGTISVQRRRIEILDNRKLAAYDE
>Mature_225_residues
MLTEKTLVAELRRHHLFSRLPDAALQEVCASANLKRLPAGASLFHQGDKADRFYFLFSGQIKLHRVVCDGQEKLVEVMRA
GESFAEALLFKGTPCYPVSATALKASLVASLNGPHYRRILEQQPDICLDILATLSIRLHQRMTEIDTLTLANASHRVVRF
LAQSQQDDSGVVVLDVPKRLIASKLGIQPETFSRILHRLIDAGTISVQRRRIEILDNRKLAAYDE

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=202, Percent_Identity=22.7722772277228, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25223; Mature: 25223

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTEKTLVAELRRHHLFSRLPDAALQEVCASANLKRLPAGASLFHQGDKADRFYFLFSGQ
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEEEEECC
IKLHRVVCDGQEKLVEVMRAGESFAEALLFKGTPCYPVSATALKASLVASLNGPHYRRIL
EEEEEEEECCHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHH
EQQPDICLDILATLSIRLHQRMTEIDTLTLANASHRVVRFLAQSQQDDSGVVVLDVPKRL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEECCCHHH
IASKLGIQPETFSRILHRLIDAGTISVQRRRIEILDNRKLAAYDE
HHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLTEKTLVAELRRHHLFSRLPDAALQEVCASANLKRLPAGASLFHQGDKADRFYFLFSGQ
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEEEEECC
IKLHRVVCDGQEKLVEVMRAGESFAEALLFKGTPCYPVSATALKASLVASLNGPHYRRIL
EEEEEEEECCHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHH
EQQPDICLDILATLSIRLHQRMTEIDTLTLANASHRVVRFLAQSQQDDSGVVVLDVPKRL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEECCCHHH
IASKLGIQPETFSRILHRLIDAGTISVQRRRIEILDNRKLAAYDE
HHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]