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The map label for this gene is smpB

Identifier: 146283652

GI number: 146283652

Start: 3608193

End: 3608675

Strand: Direct

Name: smpB

Synonym: PST_3334

Alternate gene names: 146283652

Gene position: 3608193-3608675 (Clockwise)

Preceding gene: 146283649

Following gene: 146283654

Centisome position: 79.0

GC content: 61.7

Gene sequence:

>483_bases
ATGGCCAAACAGAAGAAGCAGTCCCCCGGGACCATCGCGCTGAACAAGAAAGCGCTACACGATTACTTCATCGAGCAGAA
GTTCGAGGCCGGCCTCGTCCTTGCCGGCTGGGAAGTGAAAAGCCTGCGCGCCGGCAAGGCGCAGCTGGTGGACAGCTACG
TGCTGCTCAAGGATGGCGAAGCCTGGCTGATGGGCGCGCACATCACCCCGCTGACGAGCGCCAGCACCCATGTCATCGCC
GACCCGACCCGTACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTGGGCAAGCTGTTCGGCGCCGTGCAGCAGAAGGGCTA
TGCCTGCGTAGCGCTGTCGCTGTACTGGAAGAAGCACATGATCAAGTGCGAGATCGCCCTGGCCAAGGGCAAGAAGGAAT
TCGACAAGCGCCACACCGAGAAAGAACGCGATTCCGATCGGGAAATCCAGCGCGCCATGCGCACCAAGGGCAAGGACGAC
TGA

Upstream 100 bases:

>100_bases
GCACATTGTCGGGGATTAACGGCTGCCCCTCAAGTTTCTGCCGCCAACGCTCCTGCAGGTTGTCGCCAGCCGCAACGGGA
CTCCCTATAATGCCGCCCCT

Downstream 100 bases:

>100_bases
TCAGCTCCGGGCGGAGCGCCCACCGCCTCGCCCGCCAACCCCGCAGCATCCATCACCCCGCCTTTTGCGACGCCCCCACT
CTCTCCTGCCGGCGCTGCGC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MAKQKKQSPGTIALNKKALHDYFIEQKFEAGLVLAGWEVKSLRAGKAQLVDSYVLLKDGEAWLMGAHITPLTSASTHVIA
DPTRTRKLLLNKRELGKLFGAVQQKGYACVALSLYWKKHMIKCEIALAKGKKEFDKRHTEKERDSDREIQRAMRTKGKDD

Sequences:

>Translated_160_residues
MAKQKKQSPGTIALNKKALHDYFIEQKFEAGLVLAGWEVKSLRAGKAQLVDSYVLLKDGEAWLMGAHITPLTSASTHVIA
DPTRTRKLLLNKRELGKLFGAVQQKGYACVALSLYWKKHMIKCEIALAKGKKEFDKRHTEKERDSDREIQRAMRTKGKDD
>Mature_159_residues
AKQKKQSPGTIALNKKALHDYFIEQKFEAGLVLAGWEVKSLRAGKAQLVDSYVLLKDGEAWLMGAHITPLTSASTHVIAD
PTRTRKLLLNKRELGKLFGAVQQKGYACVALSLYWKKHMIKCEIALAKGKKEFDKRHTEKERDSDREIQRAMRTKGKDD

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=157, Percent_Identity=62.4203821656051, Blast_Score=196, Evalue=6e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_PSEU5 (A4VPR2)

Other databases:

- EMBL:   CP000304
- RefSeq:   YP_001173805.1
- ProteinModelPortal:   A4VPR2
- SMR:   A4VPR2
- STRING:   A4VPR2
- GeneID:   5096295
- GenomeReviews:   CP000304_GR
- KEGG:   psa:PST_3334
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- PhylomeDB:   A4VPR2
- ProtClustDB:   PRK05422
- BioCyc:   PSTU379731:PST_3334-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18109; Mature: 17978

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKKQSPGTIALNKKALHDYFIEQKFEAGLVLAGWEVKSLRAGKAQLVDSYVLLKDGE
CCCCCCCCCCEEEECHHHHHHHHHHHHCCCCEEEECCCHHHHHCCHHHHHHEEEEEECCC
AWLMGAHITPLTSASTHVIADPTRTRKLLLNKRELGKLFGAVQQKGYACVALSLYWKKHM
EEEEECCCCCCCCCCCEEEECCHHHHHHHHCHHHHHHHHHHHHHCCCEEEEHHHHHHHHH
IKCEIALAKGKKEFDKRHTEKERDSDREIQRAMRTKGKDD
HEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKQKKQSPGTIALNKKALHDYFIEQKFEAGLVLAGWEVKSLRAGKAQLVDSYVLLKDGE
CCCCCCCCCEEEECHHHHHHHHHHHHCCCCEEEECCCHHHHHCCHHHHHHEEEEEECCC
AWLMGAHITPLTSASTHVIADPTRTRKLLLNKRELGKLFGAVQQKGYACVALSLYWKKHM
EEEEECCCCCCCCCCCEEEECCHHHHHHHHCHHHHHHHHHHHHHCCCEEEEHHHHHHHHH
IKCEIALAKGKKEFDKRHTEKERDSDREIQRAMRTKGKDD
HEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA