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The map label for this gene is dusB [H]

Identifier: 146283597

GI number: 146283597

Start: 3543975

End: 3544988

Strand: Direct

Name: dusB [H]

Synonym: PST_3277

Alternate gene names: 146283597

Gene position: 3543975-3544988 (Clockwise)

Preceding gene: 146283596

Following gene: 146283598

Centisome position: 77.59

GC content: 64.99

Gene sequence:

>1014_bases
ATGTCAGCGGTACGCATCGGCCCCTACACCTTGACGAATCGGTTGATTCTCGCGCCCATGGCGGGCGTGACCGATCAGCC
GTTCCGCCAGTTGTGCCGCCGCTTCGGCGCGGCCCTGGAGGTCTCGGAAATGCTCACCAGCGATGTGCGCCTGTGGAACA
GCCGCAAGTCGCGGTTACGCATGCCCCATGCCGACGAACCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGACCCGCAG
ATGCTCGCCGAAGCGGCGCAGCGCAACGTCGAGCTGGGCGCACAGATCATCGATATCAACATGGGTTGCCCGGCAAAGAA
GGTCTGCAACAAGGCCGCCGGTTCGGCGCTGATGCGCGATGAGCGGCTGGTCGCGGACATTCTCGAGGCCGTGGTCGCCG
CGGTGGATGTGCCGGTGACGCTGAAGATCCGCACCGGCTGGGACCGCCAGAACAAGAATGGCGCGACCATCGCCCGCATC
GCCGAACAGTCCGGCATCGCGGCGCTGGCAGTGCACGGGCGCACCCGCGCCGACCTGTATACCGGCGAGGCCGAATACGA
CACCATCGCCGCCATCAAGCAGGCGGTGTCGATTCCGGTGTTTGCCAACGGCGACATCGATTCGCCGCATAAGGCGGCCA
GGGTGCTCCAAGTCACTGGGGCCGATGGCCTGCTGATTGGACGTGCGGCGCAGGGTCGCCCATGGATCTTTCGCGAAGTG
GAGCATTATCTGCGCACCGGCGAGTTGCTCGCGGCGCCGACGCTGGGCGAGGTCGAAAGCACCCTGCTGGAACATGTTCA
GGCGCTGCACGAATTCTATGGCGAGGTGATGGGAGTTCGTATCGCCCGCAAGCACGTGGGTTGGTATCTCGCAACACTGC
CGGGCGCGCGGGACTTCCGCGCCCGTTTCAATCATCTGGAAGACAGGGATGCGCAAGGCTCCAGCATCCGGCAATTCTTT
GCCGAACAACGCTCTGGGCCTGGCTCGGTGGATGGAACAGAGGTGGCCGCATGA

Upstream 100 bases:

>100_bases
TCAAAACAGCCTTTAACCCGTCATGCGTAGCGGGTATTATGCCCACCCTTTTTTGCAGTCCCGATCGACCTAACAAGCAG
GCCCCAAGCAGGGAATCTTC

Downstream 100 bases:

>100_bases
CGCTGTTGAATGAAACATTGGTAAGTGGAACAACATCCGTGAGTGACAACGTGAACCTCAAGCAGCACCTGAACACGCCG
AGCGAAGCAGGGCAGACGTT

Product: NifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSAVRIGPYTLTNRLILAPMAGVTDQPFRQLCRRFGAALEVSEMLTSDVRLWNSRKSRLRMPHADEPEPRSVQIAGGDPQ
MLAEAAQRNVELGAQIIDINMGCPAKKVCNKAAGSALMRDERLVADILEAVVAAVDVPVTLKIRTGWDRQNKNGATIARI
AEQSGIAALAVHGRTRADLYTGEAEYDTIAAIKQAVSIPVFANGDIDSPHKAARVLQVTGADGLLIGRAAQGRPWIFREV
EHYLRTGELLAAPTLGEVESTLLEHVQALHEFYGEVMGVRIARKHVGWYLATLPGARDFRARFNHLEDRDAQGSSIRQFF
AEQRSGPGSVDGTEVAA

Sequences:

>Translated_337_residues
MSAVRIGPYTLTNRLILAPMAGVTDQPFRQLCRRFGAALEVSEMLTSDVRLWNSRKSRLRMPHADEPEPRSVQIAGGDPQ
MLAEAAQRNVELGAQIIDINMGCPAKKVCNKAAGSALMRDERLVADILEAVVAAVDVPVTLKIRTGWDRQNKNGATIARI
AEQSGIAALAVHGRTRADLYTGEAEYDTIAAIKQAVSIPVFANGDIDSPHKAARVLQVTGADGLLIGRAAQGRPWIFREV
EHYLRTGELLAAPTLGEVESTLLEHVQALHEFYGEVMGVRIARKHVGWYLATLPGARDFRARFNHLEDRDAQGSSIRQFF
AEQRSGPGSVDGTEVAA
>Mature_336_residues
SAVRIGPYTLTNRLILAPMAGVTDQPFRQLCRRFGAALEVSEMLTSDVRLWNSRKSRLRMPHADEPEPRSVQIAGGDPQM
LAEAAQRNVELGAQIIDINMGCPAKKVCNKAAGSALMRDERLVADILEAVVAAVDVPVTLKIRTGWDRQNKNGATIARIA
EQSGIAALAVHGRTRADLYTGEAEYDTIAAIKQAVSIPVFANGDIDSPHKAARVLQVTGADGLLIGRAAQGRPWIFREVE
HYLRTGELLAAPTLGEVESTLLEHVQALHEFYGEVMGVRIARKHVGWYLATLPGARDFRARFNHLEDRDAQGSSIRQFFA
EQRSGPGSVDGTEVAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=233, Percent_Identity=33.4763948497854, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI239788462, Length=233, Percent_Identity=33.4763948497854, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=33.920704845815, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI40807366, Length=232, Percent_Identity=32.7586206896552, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI8923374, Length=244, Percent_Identity=31.9672131147541, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1789660, Length=317, Percent_Identity=61.198738170347, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1788462, Length=243, Percent_Identity=34.5679012345679, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI145693211, Length=308, Percent_Identity=26.2987012987013, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17543114, Length=240, Percent_Identity=30.8333333333333, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=223, Percent_Identity=32.7354260089686, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=258, Percent_Identity=30.6201550387597, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=246, Percent_Identity=30.8943089430894, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323433, Length=322, Percent_Identity=27.0186335403727, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6323560, Length=230, Percent_Identity=30, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=26.3157894736842, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24585320, Length=279, Percent_Identity=29.0322580645161, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24580595, Length=289, Percent_Identity=29.757785467128, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19920448, Length=289, Percent_Identity=29.757785467128, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45549423, Length=261, Percent_Identity=32.183908045977, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19921524, Length=327, Percent_Identity=24.4648318042813, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36707; Mature: 36576

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVRIGPYTLTNRLILAPMAGVTDQPFRQLCRRFGAALEVSEMLTSDVRLWNSRKSRLR
CCCEEECCHHHHCCEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHCC
MPHADEPEPRSVQIAGGDPQMLAEAAQRNVELGAQIIDINMGCPAKKVCNKAAGSALMRD
CCCCCCCCCCEEEEECCCHHHHHHHHHCCHHCCCEEEEECCCCCHHHHHHHHHHHHHHHH
ERLVADILEAVVAAVDVPVTLKIRTGWDRQNKNGATIARIAEQSGIAALAVHGRTRADLY
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCEE
TGEAEYDTIAAIKQAVSIPVFANGDIDSPHKAARVLQVTGADGLLIGRAAQGRPWIFREV
CCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHEEEEEECCCCEEEECCCCCCCHHHHHH
EHYLRTGELLAAPTLGEVESTLLEHVQALHEFYGEVMGVRIARKHVGWYLATLPGARDFR
HHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHH
ARFNHLEDRDAQGSSIRQFFAEQRSGPGSVDGTEVAA
HHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAVRIGPYTLTNRLILAPMAGVTDQPFRQLCRRFGAALEVSEMLTSDVRLWNSRKSRLR
CCEEECCHHHHCCEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHCC
MPHADEPEPRSVQIAGGDPQMLAEAAQRNVELGAQIIDINMGCPAKKVCNKAAGSALMRD
CCCCCCCCCCEEEEECCCHHHHHHHHHCCHHCCCEEEEECCCCCHHHHHHHHHHHHHHHH
ERLVADILEAVVAAVDVPVTLKIRTGWDRQNKNGATIARIAEQSGIAALAVHGRTRADLY
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCEE
TGEAEYDTIAAIKQAVSIPVFANGDIDSPHKAARVLQVTGADGLLIGRAAQGRPWIFREV
CCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHEEEEEECCCCEEEECCCCCCCHHHHHH
EHYLRTGELLAAPTLGEVESTLLEHVQALHEFYGEVMGVRIARKHVGWYLATLPGARDFR
HHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHH
ARFNHLEDRDAQGSSIRQFFAEQRSGPGSVDGTEVAA
HHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]