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The map label for this gene is lolB

Identifier: 146283512

GI number: 146283512

Start: 3432588

End: 3433208

Strand: Direct

Name: lolB

Synonym: PST_3185

Alternate gene names: 146283512

Gene position: 3432588-3433208 (Clockwise)

Preceding gene: 146283511

Following gene: 146283513

Centisome position: 75.15

GC content: 64.41

Gene sequence:

>621_bases
ATGTCACTGTTGAAAAACCTCCTTGCTCCCTGCCTGGCCCTCCTGCTTGCTGGCTGTGCCGCCCTGGGCCCACACGAATC
GGTTGAAGGCCCCGGCAATACCGCGGCCTGGAAGGAGCACCGTAGCCATGTAGCAACGCTTGATGGCTGGCAGATCAGCG
GCAAGATCGGCATTCGCGCACCACAGGAATCCGGCAGCGGCACGCTGTTCTGGCTGCAACGCCAAGACTACTTTGACATC
CGCCTGTCCGGCCCCCTAGGCCGCGGCGCCACGAGACTCACCGGCCGACCGGATGCCGTGTCGCTGGAAGTTGCCGGCCA
GGGTCGCTTCGAAGCGGAGTCCCCCGAAGCGTTGGTCGAAGCTCAACTCGGCTGGCAGTTGCCGGTCTCCCACCTGCTCT
GGTGGGTGCGCGGGCTGCCAGCACCGGACAGCCGCAGTCGCTTGAGCATAGACAGCGAGAGCCGCCTGGCGCGTCTTGAG
CAGGACGGCTGGCAGGTTGAGTATCTAGCCTATGCCGAACACAACGGTTTCGTCCTGCCCGAGCGCATCCGGCTACAAGG
GCACGATCTGCAGATCACCCTGGTGATCAAGGACTGGCAACCCCGCCAGCTGGGCCGTTGA

Upstream 100 bases:

>100_bases
GGCCCGCGCGGTCTGGTCGAAGGCACTCAAACTACAACCGGACAGCCCGATCTTGCTCGAGACGATCAAGCGGCTGACCG
GATCGGAGAAGCTCTGACCC

Downstream 100 bases:

>100_bases
ATGCTGACGCTACCAGCGCCCGCCAAACTCAATCTGATGCTGCACATAGTCGGGCGCCGCGCCGACGGTTACCACGAGTT
GCAGACGCTATTCCAGTTTC

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MSLLKNLLAPCLALLLAGCAALGPHESVEGPGNTAAWKEHRSHVATLDGWQISGKIGIRAPQESGSGTLFWLQRQDYFDI
RLSGPLGRGATRLTGRPDAVSLEVAGQGRFEAESPEALVEAQLGWQLPVSHLLWWVRGLPAPDSRSRLSIDSESRLARLE
QDGWQVEYLAYAEHNGFVLPERIRLQGHDLQITLVIKDWQPRQLGR

Sequences:

>Translated_206_residues
MSLLKNLLAPCLALLLAGCAALGPHESVEGPGNTAAWKEHRSHVATLDGWQISGKIGIRAPQESGSGTLFWLQRQDYFDI
RLSGPLGRGATRLTGRPDAVSLEVAGQGRFEAESPEALVEAQLGWQLPVSHLLWWVRGLPAPDSRSRLSIDSESRLARLE
QDGWQVEYLAYAEHNGFVLPERIRLQGHDLQITLVIKDWQPRQLGR
>Mature_205_residues
SLLKNLLAPCLALLLAGCAALGPHESVEGPGNTAAWKEHRSHVATLDGWQISGKIGIRAPQESGSGTLFWLQRQDYFDIR
LSGPLGRGATRLTGRPDAVSLEVAGQGRFEAESPEALVEAQLGWQLPVSHLLWWVRGLPAPDSRSRLSIDSESRLARLEQ
DGWQVEYLAYAEHNGFVLPERIRLQGHDLQITLVIKDWQPRQLGR

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family

Homologues:

Organism=Escherichia coli, GI1787460, Length=200, Percent_Identity=27.5, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LOLB_PSEU5 (A4VPC2)

Other databases:

- EMBL:   CP000304
- RefSeq:   YP_001173665.1
- ProteinModelPortal:   A4VPC2
- SMR:   A4VPC2
- STRING:   A4VPC2
- GeneID:   5096146
- GenomeReviews:   CP000304_GR
- KEGG:   psa:PST_3185
- eggNOG:   COG3017
- HOGENOM:   HBG645031
- OMA:   WLQRQDY
- PhylomeDB:   A4VPC2
- ProtClustDB:   PRK00022
- BioCyc:   PSTU379731:PST_3185-MONOMER
- HAMAP:   MF_00233
- InterPro:   IPR004565
- TIGRFAMs:   TIGR00548

Pfam domain/function: PF03550 LolB

EC number: NA

Molecular weight: Translated: 22797; Mature: 22666

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLKNLLAPCLALLLAGCAALGPHESVEGPGNTAAWKEHRSHVATLDGWQISGKIGIRA
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCEEEEECCEEEECEEEEEC
PQESGSGTLFWLQRQDYFDIRLSGPLGRGATRLTGRPDAVSLEVAGQGRFEAESPEALVE
CCCCCCEEEEEEECCCEEEEEECCCCCCCCEECCCCCCEEEEEECCCCCCCCCCCHHHHH
AQLGWQLPVSHLLWWVRGLPAPDSRSRLSIDSESRLARLEQDGWQVEYLAYAEHNGFVLP
HHCCCCCCHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHCCCCEEEEEEEECCCCEECC
ERIRLQGHDLQITLVIKDWQPRQLGR
HHHEECCCCEEEEEEEECCCCCCCCC
>Mature Secondary Structure 
SLLKNLLAPCLALLLAGCAALGPHESVEGPGNTAAWKEHRSHVATLDGWQISGKIGIRA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCEEEEECCEEEECEEEEEC
PQESGSGTLFWLQRQDYFDIRLSGPLGRGATRLTGRPDAVSLEVAGQGRFEAESPEALVE
CCCCCCEEEEEEECCCEEEEEECCCCCCCCEECCCCCCEEEEEECCCCCCCCCCCHHHHH
AQLGWQLPVSHLLWWVRGLPAPDSRSRLSIDSESRLARLEQDGWQVEYLAYAEHNGFVLP
HHCCCCCCHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHCCCCEEEEEEEECCCCEECC
ERIRLQGHDLQITLVIKDWQPRQLGR
HHHEECCCCEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA