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The map label for this gene is uraA [H]

Identifier: 146283503

GI number: 146283503

Start: 3423568

End: 3424827

Strand: Direct

Name: uraA [H]

Synonym: PST_3176

Alternate gene names: 146283503

Gene position: 3423568-3424827 (Clockwise)

Preceding gene: 146283502

Following gene: 146283504

Centisome position: 74.96

GC content: 60.95

Gene sequence:

>1260_bases
ATGTCGCACACGGATGAGCCGCTTGGCCAACAGGTACTGGCCGGGGCGCAGATGCTATTTGTCGCCTTCGGCGCACTGGT
ACTGATGCCGCTGATTACCGGCATGGACCCTAACGTCGCATTGTTTACTGCAGGGTTGGGCACGCTGTTGTTCCAAGTGG
TCACGCGGCGCCAGGTTCCGGTTTTTCTGGCATCCAGCTTCGCTTTCATCGCGCCGATCATCGCTGCCAAGGGCGAGTTC
GGCTTACCCGCCGTCATGGGCGGCGTGGTAGCGGCTGGCTTCGTCTACATCCTTCTCAGCTTTGCTGTACGCCTGAAGGG
GCCGGGATTCATCGACCGCTTGTTGCCACCGGTGGTGATCGCTCCGGTGATCATTTCCATTGGCCTGGCACTCTCGCCAG
TGGCAGTAAACATGGCCATGGGCAAAGCCGGCGACGGCAGCGCACAACTGATCCCTTATCAGACGGCGATGCTCATCTCC
ATGCCCGCTCTGGTCACTACCTTGCTGGTAGCCGTACTGGGCAAGGGCTTGTTTCGTCTGGTGCCGATTCTTGCGGGCGT
TGCGGTCGGCTATGGGCTCTCTTTCGCGTTTGGCGTAGTCGACACCAGCGGAATTGCCGCGGCGCCCTGGCTGGCGATAC
CCAACTTCGTCGCGCCCGAATTCCACATTGGCGCCATTCTGTTCATGGTCCCGGTGGCACTCGCACCGGCCATCGAGCAC
ATCGGCGGCGTGGTTGCCATCGGCAGCGTCACGGGCAACAACTACGTTAAGAATCCCGGCCTGCATCGCACGTTGCTGGG
CGATGGGGTGGCGACCTCGGCGGCCGGCCTGTTCGGTGGCCCACCGAACACCACCTATGCCGAAGTGACCGGCGCCGTCA
TGCTGACCAAGAACTACAACCCGAAGATCATGACGTGGGCGGCGGTTTTCGCCATCACGCTTGCCTTCATCGGCAAGTTC
GGCGCTGGGCTGCTGAGCATTCCGATCCCGGTGATGGGCGGCATACTCTGCCTACTGTTCGGCTCGATCGCCGTTGTCGG
TCTGAGCACACTTATCCGCCATCAGGTGGACCTCTCCGAAGCACGCAACCTCATCATCGTCTCGGTGACACTGGTGTTCG
GCATCGGAGGCATGGCCATCGGCCATGGCGAGTTCACCTTGTCCGGCATTTCGCTATGCGCGATCGTCGCTCTATTGCTC
AACCTGCTGCTGCCTGGCGGTCAGGGTTGGCGCAACAAACAATTGAACGACGAGCTTTGA

Upstream 100 bases:

>100_bases
CCTCCATCGACGAGCGTCTCGACGAGCACGGATACATCGTTCCAGGCCTGGGCGATGCAGGCGACAAGATCTTCGGCACC
AAACAGAAGGACATCTGAGC

Downstream 100 bases:

>100_bases
AGCACGGGGCACCAGCTGTGGCTGATGCCCCGCCACCTGCTGTCAGCGCCGCTCCCTCCCGGTTGATCATATCGACTAGA
CTGCGGCGATGAACGACTCG

Product: uracil permease

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSHTDEPLGQQVLAGAQMLFVAFGALVLMPLITGMDPNVALFTAGLGTLLFQVVTRRQVPVFLASSFAFIAPIIAAKGEF
GLPAVMGGVVAAGFVYILLSFAVRLKGPGFIDRLLPPVVIAPVIISIGLALSPVAVNMAMGKAGDGSAQLIPYQTAMLIS
MPALVTTLLVAVLGKGLFRLVPILAGVAVGYGLSFAFGVVDTSGIAAAPWLAIPNFVAPEFHIGAILFMVPVALAPAIEH
IGGVVAIGSVTGNNYVKNPGLHRTLLGDGVATSAAGLFGGPPNTTYAEVTGAVMLTKNYNPKIMTWAAVFAITLAFIGKF
GAGLLSIPIPVMGGILCLLFGSIAVVGLSTLIRHQVDLSEARNLIIVSVTLVFGIGGMAIGHGEFTLSGISLCAIVALLL
NLLLPGGQGWRNKQLNDEL

Sequences:

>Translated_419_residues
MSHTDEPLGQQVLAGAQMLFVAFGALVLMPLITGMDPNVALFTAGLGTLLFQVVTRRQVPVFLASSFAFIAPIIAAKGEF
GLPAVMGGVVAAGFVYILLSFAVRLKGPGFIDRLLPPVVIAPVIISIGLALSPVAVNMAMGKAGDGSAQLIPYQTAMLIS
MPALVTTLLVAVLGKGLFRLVPILAGVAVGYGLSFAFGVVDTSGIAAAPWLAIPNFVAPEFHIGAILFMVPVALAPAIEH
IGGVVAIGSVTGNNYVKNPGLHRTLLGDGVATSAAGLFGGPPNTTYAEVTGAVMLTKNYNPKIMTWAAVFAITLAFIGKF
GAGLLSIPIPVMGGILCLLFGSIAVVGLSTLIRHQVDLSEARNLIIVSVTLVFGIGGMAIGHGEFTLSGISLCAIVALLL
NLLLPGGQGWRNKQLNDEL
>Mature_418_residues
SHTDEPLGQQVLAGAQMLFVAFGALVLMPLITGMDPNVALFTAGLGTLLFQVVTRRQVPVFLASSFAFIAPIIAAKGEFG
LPAVMGGVVAAGFVYILLSFAVRLKGPGFIDRLLPPVVIAPVIISIGLALSPVAVNMAMGKAGDGSAQLIPYQTAMLISM
PALVTTLLVAVLGKGLFRLVPILAGVAVGYGLSFAFGVVDTSGIAAAPWLAIPNFVAPEFHIGAILFMVPVALAPAIEHI
GGVVAIGSVTGNNYVKNPGLHRTLLGDGVATSAAGLFGGPPNTTYAEVTGAVMLTKNYNPKIMTWAAVFAITLAFIGKFG
AGLLSIPIPVMGGILCLLFGSIAVVGLSTLIRHQVDLSEARNLIIVSVTLVFGIGGMAIGHGEFTLSGISLCAIVALLLN
LLLPGGQGWRNKQLNDEL

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680145, Length=433, Percent_Identity=25.8660508083141, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI44680143, Length=437, Percent_Identity=25.629290617849, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI44680148, Length=394, Percent_Identity=24.6192893401015, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI40316845, Length=394, Percent_Identity=24.6192893401015, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1788843, Length=397, Percent_Identity=38.7909319899244, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI87081818, Length=418, Percent_Identity=38.755980861244, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI87082181, Length=433, Percent_Identity=29.3302540415704, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87082178, Length=320, Percent_Identity=26.25, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17558856, Length=203, Percent_Identity=29.064039408867, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17542262, Length=224, Percent_Identity=26.7857142857143, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21356175, Length=296, Percent_Identity=29.7297297297297, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 43069; Mature: 42938

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCEEEEHHHHHHCCCCCEECCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SHTDEPLGQQVLAGAQMLFVAFGALVLMPLITGMDPNVALFTAGLGTLLFQVVTRRQVP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCC
VFLASSFAFIAPIIAAKGEFGLPAVMGGVVAAGFVYILLSFAVRLKGPGFIDRLLPPVVI
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
APVIISIGLALSPVAVNMAMGKAGDGSAQLIPYQTAMLISMPALVTTLLVAVLGKGLFRL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPILAGVAVGYGLSFAFGVVDTSGIAAAPWLAIPNFVAPEFHIGAILFMVPVALAPAIEH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IGGVVAIGSVTGNNYVKNPGLHRTLLGDGVATSAAGLFGGPPNTTYAEVTGAVMLTKNYN
CCCEEEEEEECCCCCCCCCCCCHHHHCCCHHHHHCCCCCCCCCCCHHHHHEEEEEECCCC
PKIMTWAAVFAITLAFIGKFGAGLLSIPIPVMGGILCLLFGSIAVVGLSTLIRHQVDLSE
CCHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
ARNLIIVSVTLVFGIGGMAIGHGEFTLSGISLCAIVALLLNLLLPGGQGWRNKQLNDEL
CCCEEEEHHHHHHCCCCCEECCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]