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The map label for this gene is uao [H]

Identifier: 146283421

GI number: 146283421

Start: 3328535

End: 3329050

Strand: Direct

Name: uao [H]

Synonym: PST_3094

Alternate gene names: 146283421

Gene position: 3328535-3329050 (Clockwise)

Preceding gene: 146283420

Following gene: 146283422

Centisome position: 72.88

GC content: 65.5

Gene sequence:

>516_bases
ATGACCCCGTTCAAGACGATCAAGCCCTCGACCCTCGACCGCGATGCCTTCGTCGCCGCCTTCGCCGATGTCTACGAGCA
TTCGCCCTGGGTCGCCGAGACGGTCTATGGCGCTGGCGTCGACGAGACGCTGGACTACGTCGAGGTGATGCACACCCGCC
TGTCCCAGGCGATGCTCGCCGCCGACCACGACACCCAGCTCGCGCTGGTCAATGCGCACCCGGATCTGGCCGGCAAGGCG
GCCGTGCGCGGCGAGCTGACTGCCGCCAGCACCTCCGAACAGGCAGGCGCCGGCATCCATGAGTGCACGCCCGAGGAGTT
CGCCCGTTTCACCGAACTCAACGACGCCTACAAGGCGAAATTCGGCTTCCCCTTCATCATGGCCGTCAAGGGCAGCAACC
GGCACCAGATCCTCGCCGCGTTCGAGGAGCGCATCCACAACAGCCCGGAACAGGAATTCGCCCGGGCACTGGCGGAAATC
AACAAGATCGCCCTGTTCCGCCTGCAGGCCATGTAG

Upstream 100 bases:

>100_bases
TCGAATACGCCAAGGGGCATGACAAGGTCTGGTTTGCCCGCCGCGTCGATATCGCCCGCCACTGGCACGCCACTCACCCC
ATGACCGCGGAGCGCAATTG

Downstream 100 bases:

>100_bases
CCGGAAAGGGACCGGCAACACCGCACTACAGGCTGCAAGGGCAGAACGCTCCACTCCATTTCAAGGCAGATCGCGATGAA
AGCTTACGCCGTACCCTTCG

Product: hypothetical protein

Products: NA

Alternate protein names: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase; OHCU decarboxylase; Uricase; Urate oxidase [H]

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MTPFKTIKPSTLDRDAFVAAFADVYEHSPWVAETVYGAGVDETLDYVEVMHTRLSQAMLAADHDTQLALVNAHPDLAGKA
AVRGELTAASTSEQAGAGIHECTPEEFARFTELNDAYKAKFGFPFIMAVKGSNRHQILAAFEERIHNSPEQEFARALAEI
NKIALFRLQAM

Sequences:

>Translated_171_residues
MTPFKTIKPSTLDRDAFVAAFADVYEHSPWVAETVYGAGVDETLDYVEVMHTRLSQAMLAADHDTQLALVNAHPDLAGKA
AVRGELTAASTSEQAGAGIHECTPEEFARFTELNDAYKAKFGFPFIMAVKGSNRHQILAAFEERIHNSPEQEFARALAEI
NKIALFRLQAM
>Mature_170_residues
TPFKTIKPSTLDRDAFVAAFADVYEHSPWVAETVYGAGVDETLDYVEVMHTRLSQAMLAADHDTQLALVNAHPDLAGKAA
VRGELTAASTSEQAGAGIHECTPEEFARFTELNDAYKAKFGFPFIMAVKGSNRHQILAAFEERIHNSPEQEFARALAEIN
KIALFRLQAM

Specific function: Catalyzes two steps in the degradation of uric acid, i.e. the oxidation of uric acid to 5-hydroxyisourate (HIU) and the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5- ureidoimidazoline (OHCU) to (S)-allantoin [H]

COG id: COG3195

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the uricase family [H]

Homologues:

Organism=Homo sapiens, GI157743280, Length=161, Percent_Identity=33.5403726708075, Blast_Score=94, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018020
- InterPro:   IPR017580
- InterPro:   IPR002042
- InterPro:   IPR019842 [H]

Pfam domain/function: PF09349 OHCU_decarbox; PF01014 Uricase [H]

EC number: =1.7.3.3 [H]

Molecular weight: Translated: 18827; Mature: 18696

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPFKTIKPSTLDRDAFVAAFADVYEHSPWVAETVYGAGVDETLDYVEVMHTRLSQAMLA
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ADHDTQLALVNAHPDLAGKAAVRGELTAASTSEQAGAGIHECTPEEFARFTELNDAYKAK
CCCCCEEEEEECCCCCCCCHHHCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHH
FGFPFIMAVKGSNRHQILAAFEERIHNSPEQEFARALAEINKIALFRLQAM
CCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECC
>Mature Secondary Structure 
TPFKTIKPSTLDRDAFVAAFADVYEHSPWVAETVYGAGVDETLDYVEVMHTRLSQAMLA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ADHDTQLALVNAHPDLAGKAAVRGELTAASTSEQAGAGIHECTPEEFARFTELNDAYKAK
CCCCCEEEEEECCCCCCCCHHHCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHH
FGFPFIMAVKGSNRHQILAAFEERIHNSPEQEFARALAEINKIALFRLQAM
CCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8907179 [H]