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The map label for this gene is rlmF

Identifier: 146283395

GI number: 146283395

Start: 3297398

End: 3298381

Strand: Direct

Name: rlmF

Synonym: PST_3066

Alternate gene names: 146283395

Gene position: 3297398-3298381 (Clockwise)

Preceding gene: 146283394

Following gene: 146283399

Centisome position: 72.19

GC content: 66.67

Gene sequence:

>984_bases
ATGCCACGCAAAACCTCGTCCCAGCCGCGCCCTGCCGAGCCGAAGGCCGTGCTGCATCCACGCAACCGCCACAGCGGCCG
CTACGACTTTCCCAAGCTGATCGCCGCCTGCCCGGAGCTGGGCGAGTACGTCATCCTCAATCCCTACGGCAAGCAGAGCA
TCGACTTCGCCAATCCGGATGCGGTACGGGTGTTCAACCGCGCACTGCTTCGGCAGTTCTACGGCATCCAGCACTGGGAC
ATTCCTGCCGGCTACCTCTGCCCGCCGGTGCCGGGGCGGGCGGATTACCTGCACGGCCTGGCCGACCTGCTGGCCGCCGA
TAACGCCGGCGTCATCCCGCGCGGGGCCGCGGTCCGGGTGCTCGATATCGGCACCGGCGCCAACTGCATCTACCCGCTGA
TCGGCCATCGCGAATATGGCTGGCGCTTCACTGGCAGCGACATCGACGCCACGGCGCTGGCCTCGGCGCGCACCATCGTC
GCGGCCAACAGGCTAGACAAGGTCATCGAATTGCGCCGGCAGGACACCTCGACGCAGCTCTTCAAGGGCATCCTGGCGGC
GGACGAGCGCTTCGAGTTGACCCTGTGCAATCCGCCATTCCACGCCTCGCAGGCCGAGGCTGCCAGCGGCAGTCAGCGCA
AGTGGCGCAATCTCGGCAAGCTGGACCCGAGCCGCAAGCTGCCGAAGCTGAACTTCGGCGGCCAGGCGGCAGAGCTCTGG
TGCGAGGGTGGCGAGGCGGCATTTGTTGCGCGCATGGCGGACGAGAGCGTGGCGTACGCCAAGCAGGTCTACCGGTTCAG
TACGCTGGTATCGAAGGGTGGCAACGTGGCACCGCTGATGGTGCGCTTGCAACGCCTGGGTGCGCTGCAGGTTCAGGCGC
TGGATATGGCCCAGGGGCAAAAGAAAAGTCGCTTCGTCACCTGGACTTTTCTCGATGCAGCCGAGCAGGCTGCCTGGCGC
ACCGAGCGCTGGCCGCGGTCCTGA

Upstream 100 bases:

>100_bases
CTGGGGGCTACTGTGGATGCTGATGGTGCCGCGCCGGCACTAGCCAGCCGAGGCGGTTGTGGGACAATGCGCGCCCAGCC
GCCAACCTCCAGTCGATCCG

Downstream 100 bases:

>100_bases
CCGCGGCGACGCTCAACGCCGTAGATGCATCAGACGGCAATATCCCAGACCTGGCGGTCTTTGAGCGCCAGCGTACGGAT
GTAGCGCGGCTCGCCGTTGA

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MPRKTSSQPRPAEPKAVLHPRNRHSGRYDFPKLIAACPELGEYVILNPYGKQSIDFANPDAVRVFNRALLRQFYGIQHWD
IPAGYLCPPVPGRADYLHGLADLLAADNAGVIPRGAAVRVLDIGTGANCIYPLIGHREYGWRFTGSDIDATALASARTIV
AANRLDKVIELRRQDTSTQLFKGILAADERFELTLCNPPFHASQAEAASGSQRKWRNLGKLDPSRKLPKLNFGGQAAELW
CEGGEAAFVARMADESVAYAKQVYRFSTLVSKGGNVAPLMVRLQRLGALQVQALDMAQGQKKSRFVTWTFLDAAEQAAWR
TERWPRS

Sequences:

>Translated_327_residues
MPRKTSSQPRPAEPKAVLHPRNRHSGRYDFPKLIAACPELGEYVILNPYGKQSIDFANPDAVRVFNRALLRQFYGIQHWD
IPAGYLCPPVPGRADYLHGLADLLAADNAGVIPRGAAVRVLDIGTGANCIYPLIGHREYGWRFTGSDIDATALASARTIV
AANRLDKVIELRRQDTSTQLFKGILAADERFELTLCNPPFHASQAEAASGSQRKWRNLGKLDPSRKLPKLNFGGQAAELW
CEGGEAAFVARMADESVAYAKQVYRFSTLVSKGGNVAPLMVRLQRLGALQVQALDMAQGQKKSRFVTWTFLDAAEQAAWR
TERWPRS
>Mature_326_residues
PRKTSSQPRPAEPKAVLHPRNRHSGRYDFPKLIAACPELGEYVILNPYGKQSIDFANPDAVRVFNRALLRQFYGIQHWDI
PAGYLCPPVPGRADYLHGLADLLAADNAGVIPRGAAVRVLDIGTGANCIYPLIGHREYGWRFTGSDIDATALASARTIVA
ANRLDKVIELRRQDTSTQLFKGILAADERFELTLCNPPFHASQAEAASGSQRKWRNLGKLDPSRKLPKLNFGGQAAELWC
EGGEAAFVARMADESVAYAKQVYRFSTLVSKGGNVAPLMVRLQRLGALQVQALDMAQGQKKSRFVTWTFLDAAEQAAWRT
ERWPRS

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family

Homologues:

Organism=Homo sapiens, GI122114654, Length=297, Percent_Identity=31.986531986532, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI87081786, Length=299, Percent_Identity=55.5183946488294, Blast_Score=340, Evalue=6e-95,
Organism=Caenorhabditis elegans, GI32564931, Length=304, Percent_Identity=30.9210526315789, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32564933, Length=304, Percent_Identity=27.9605263157895, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI281363415, Length=297, Percent_Identity=28.2828282828283, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI19922302, Length=297, Percent_Identity=28.2828282828283, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMF_PSEU5 (A4VP05)

Other databases:

- EMBL:   CP000304
- RefSeq:   YP_001173548.1
- ProteinModelPortal:   A4VP05
- STRING:   A4VP05
- GeneID:   5096028
- GenomeReviews:   CP000304_GR
- KEGG:   psa:PST_3066
- eggNOG:   COG3129
- HOGENOM:   HBG747880
- OMA:   AELWCEG
- PhylomeDB:   A4VP05
- ProtClustDB:   PRK11727
- BioCyc:   PSTU379731:PST_3066-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01848
- InterPro:   IPR016909
- InterPro:   IPR010286
- PANTHER:   PTHR13393
- PIRSF:   PIRSF029038

Pfam domain/function: PF05971 Methyltransf_10

EC number: =2.1.1.181

Molecular weight: Translated: 36200; Mature: 36069

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRKTSSQPRPAEPKAVLHPRNRHSGRYDFPKLIAACPELGEYVILNPYGKQSIDFANPD
CCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCH
AVRVFNRALLRQFYGIQHWDIPAGYLCPPVPGRADYLHGLADLLAADNAGVIPRGAAVRV
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEE
LDIGTGANCIYPLIGHREYGWRFTGSDIDATALASARTIVAANRLDKVIELRRQDTSTQL
EEECCCCCHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
FKGILAADERFELTLCNPPFHASQAEAASGSQRKWRNLGKLDPSRKLPKLNFGGQAAELW
HHHHHHCCCCEEEEECCCCCCCCCCHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHEEE
CEGGEAAFVARMADESVAYAKQVYRFSTLVSKGGNVAPLMVRLQRLGALQVQALDMAQGQ
ECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHEEHHHHHCCC
KKSRFVTWTFLDAAEQAAWRTERWPRS
CCCEEEEEEEHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PRKTSSQPRPAEPKAVLHPRNRHSGRYDFPKLIAACPELGEYVILNPYGKQSIDFANPD
CCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCH
AVRVFNRALLRQFYGIQHWDIPAGYLCPPVPGRADYLHGLADLLAADNAGVIPRGAAVRV
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEE
LDIGTGANCIYPLIGHREYGWRFTGSDIDATALASARTIVAANRLDKVIELRRQDTSTQL
EEECCCCCHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
FKGILAADERFELTLCNPPFHASQAEAASGSQRKWRNLGKLDPSRKLPKLNFGGQAAELW
HHHHHHCCCCEEEEECCCCCCCCCCHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHEEE
CEGGEAAFVARMADESVAYAKQVYRFSTLVSKGGNVAPLMVRLQRLGALQVQALDMAQGQ
ECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHEEHHHHHCCC
KKSRFVTWTFLDAAEQAAWRTERWPRS
CCCEEEEEEEHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA