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The map label for this gene is holC [H]

Identifier: 146283388

GI number: 146283388

Start: 3288335

End: 3288754

Strand: Direct

Name: holC [H]

Synonym: PST_3059

Alternate gene names: 146283388

Gene position: 3288335-3288754 (Clockwise)

Preceding gene: 146283387

Following gene: 146283389

Centisome position: 72.0

GC content: 63.81

Gene sequence:

>420_bases
ATGACACGCATCGAGTTCTACGTGCTGCCGGACGACAACCCGCTCGGGCGGCTGCGCGCGGCCTGCCAGCTGGCAGCCAA
AGGCTGGCAACATGGCCTGCAGGTATTCGTCCGCTGCAGTGACGAGGACCAGTCGACCCGGCTCGACGAGCTGCTCTGGA
GCTTTCGCGCGGAACGTTTCATCCCGCACGAGCAGTACGCCGAGGAGGTGCAGGCGCCAGTGGTCATCGGGCTCGAACAG
GAACCACCGACCGCCCAGGGCCTGCTGATCAACCTGGCACCGACACTCTCGGCGCATATCGACCGTTTCAGCCGTGTCAT
CGAGATCGTCAACCAGGAGCCGCAGCTGCTGACCGCCTGCCGGGAGAATTTTCGCCTGTATCGGCGCCAGGGCTATGATC
CACAAAGGGTCGAGCTCTAA

Upstream 100 bases:

>100_bases
ACCCAGTATCTGCTGGACCGGATCAACGGATGAGCCCGATCACCGCGCGTGACAGCGGCCGACACGGCCCCGCGCCCGCC
GGGCCGGCGGGCGCACGTCC

Downstream 100 bases:

>100_bases
CCGCTCCAATCTTTAGACAGCAAGGGATACCCCCTGGCAAACAGCCGGGATGCACCAGCCCGGAACCACGCAGATGACAC
CGAAAGCCCCGAACAAACCC

Product: DNA polymerase III, chi subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 139; Mature: 138

Protein sequence:

>139_residues
MTRIEFYVLPDDNPLGRLRAACQLAAKGWQHGLQVFVRCSDEDQSTRLDELLWSFRAERFIPHEQYAEEVQAPVVIGLEQ
EPPTAQGLLINLAPTLSAHIDRFSRVIEIVNQEPQLLTACRENFRLYRRQGYDPQRVEL

Sequences:

>Translated_139_residues
MTRIEFYVLPDDNPLGRLRAACQLAAKGWQHGLQVFVRCSDEDQSTRLDELLWSFRAERFIPHEQYAEEVQAPVVIGLEQ
EPPTAQGLLINLAPTLSAHIDRFSRVIEIVNQEPQLLTACRENFRLYRRQGYDPQRVEL
>Mature_138_residues
TRIEFYVLPDDNPLGRLRAACQLAAKGWQHGLQVFVRCSDEDQSTRLDELLWSFRAERFIPHEQYAEEVQAPVVIGLEQE
PPTAQGLLINLAPTLSAHIDRFSRVIEIVNQEPQLLTACRENFRLYRRQGYDPQRVEL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG2927

COG function: function code L; DNA polymerase III, chi subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790709, Length=140, Percent_Identity=33.5714285714286, Blast_Score=80, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007459 [H]

Pfam domain/function: PF04364 DNA_pol3_chi [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 16119; Mature: 15987

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIEFYVLPDDNPLGRLRAACQLAAKGWQHGLQVFVRCSDEDQSTRLDELLWSFRAERF
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHC
IPHEQYAEEVQAPVVIGLEQEPPTAQGLLINLAPTLSAHIDRFSRVIEIVNQEPQLLTAC
CCHHHHHHHHCCCEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCHHHHHHH
RENFRLYRRQGYDPQRVEL
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TRIEFYVLPDDNPLGRLRAACQLAAKGWQHGLQVFVRCSDEDQSTRLDELLWSFRAERF
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHC
IPHEQYAEEVQAPVVIGLEQEPPTAQGLLINLAPTLSAHIDRFSRVIEIVNQEPQLLTAC
CCHHHHHHHHCCCEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCHHHHHHH
RENFRLYRRQGYDPQRVEL
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9864319; 10984043 [H]